Cytokines

Interleukin-4 (IL-4), also known as B cell-stimulatory factor-1, is a monomeric, approximately 13 kDa-18 kDa Th2 cytokine that shows pleiotropic effects during immune responses. It is a glycosylated polypeptide that contains three intrachain disulfide bridges and adopts a bundled four alpha -helix structure. Rhesus IL-4 is synthesized with a 24 amino acid (aa) signal sequence. Mature rhesus IL-4 shares 97%, 93%, and 93% aa sequence identity with baboon, chimpanzee, and human IL-4, respectively, and 39% -50% aa sequence identity with bovine, mouse, and rat IL-4. IL-4 exerts its effects through two receptor complexes. The type I receptor, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 R alpha and the common gamma chain (a shared subunit of the receptors for IL-2, -7, -9, -15, and -21). The type II receptor on nonhematopoietic cells consists of IL-4 R alpha and IL-13 R alpha 1. The type II receptor also transduces IL-13 mediated signals. IL-4 is primarily expressed by Th2-biased CD4+ T cells, mast cells, basophils, and eosinophils. It promotes cell proliferation, survival, and immunoglobulin class switch to IgE in B cells, acquisition of the Th2 phenotype by naïve CD4+ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells. IL-4 plays a dominant role in the development of allergic inflammation and asthma. Product Properties   Synonyms BSF-1, Lymphocyte Stimulatory Factor 1 Source E.coli-derived Rhesus Macaque IL-4, His25-Ser153. AA sequence HNCHIALREI IETLNSLTEQ KTLCTKLTIT DILAASKNTT EKETFCRAAT VLRQFYSHHE KDTRCLGATA QQFHRHKQLI RFLKRLDRNL WGLAGLNSCP VKEANQSTLE DFLERLKTIM REKYSKCSS Endotoxin < 1.0 EU per μg by the LAL method. Purity > 96% by SDS-PAGE and HPLC analyses. Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 2 × PBS, pH 7.4, 5% trehalose. Applications ELISA, Kinetics (BLI), Kinetics (SPR), Immunization Dilution Dilute with sterile distilled water or aqueous buffer containing 0.1% BSA.   Storage   The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.   Cautions   1. Please operate with lab coats and disposable gloves,for your safety.        2. This product is for research use only.  

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Interleukin-1 alpha (IL1a) is also known as IL-1A, IL1, and is a cytokine of the interleukin-1 family. Among various species, the amino acid sequence of mature IL-1 alpha is conserved 60% to 70% and human IL-1 has been found to be biologically active on murine cell lines. It possesses metabolic, physiological, haematopoietic activities, and plays one of the central roles in the regulation of the immune responses. IL-1 is a major immunoregulatory/proinflammatory cytokine which also affects fibroblast proliferation and function and therefore it was of interest to investigate whether its constitutive expression influences the in vivo tumorigenic potential of transformed fibroblastoid cell lines. Product Properties   Synonyms BAF, IL-1F1, LAF, LEM, preinterleukin 1 alpha, pro-interleukin-1-alpha Source E.coli-derived Rhesus Macaque IL-1α, Ser113-Ala271. AA sequence SAPFSFLSNM TYHFIRIIKH EFILNDTLNQ TIIRANDQHL TAAAIHNLDE AVKFDMGAYT SSKDDTKVPV ILRISKTQLY VSAQDEDQPV LLKEMPEINK TITGSETNFL FFWETHGTKN YFISVAHPNL FIATKHDNWV CLAKGLPSIT DFQILENQA Endotoxin < 1.0 EU per μg by the LAL method. Purity > 97% by SDS-PAGE and HPLC analyses. Formulation Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. Applications ELISA, Kinetics (BLI), Kinetics (SPR), Immunization Dilution Dilute with sterile distilled water or aqueous buffer containing 0.1% BSA.   Storage   The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.   Cautions   1. Please operate with lab coats and disposable gloves,for your safety.        2. This product is for research use only.  

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Interleukin-1 beta (IL-1β) is a pro inflammatory cytokine, which is secreted by immune cells to trigger inflammation and this has been found profoundly in the lesions caused by Leishmania pathogens. IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 21% amino acid (aa) identity in human. Both IL-1α and IL-1β binds to the same receptor and has similar but not identical biological properties; The 17 kDa mature rhesus IL-1 beta shares 96% aa sequence identity with human and 67% - 78% with canine, cotton rat, equine, feline, mouse, porcine, and rat IL-1 beta, are known to modulate effects of neurotoxic neurotransmitters discharged during excitation or inflammation in the central nervous system (CNS). Product Properties   Synonyms Catabolin, Leukocyte Endogenous Mediator, LEM, Lymphocyte-activating factor , LAF, Mononuclear Cell Factor, MCF , Endogenous Pyrogen, EP Source E.coli-derived Rhesus Macaque IL-1β, Ala117-Ser269. AA sequence APVRSLHCTL RDAQLKSLVM SGPYELKALH LQGQDLEQQV VFSMSFVQGE ESNDKIPVAL GLKAKNLYLS CVLKDDKPTL QLESVDPKNY PKKKMEKRFV FNKIEINNKL EFESAQFPNW YISTSQAENM PVFLGGTRGG QDITDFTMQF VSS Endotoxin < 1.0 EU per μg by the LAL method. Purity > 98% by SDS-PAGE and HPLC analyses. Formulation Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. Applications ELISA, Kinetics (BLI), Kinetics (SPR), Immunization Dilution Dilute with sterile distilled water or aqueous buffer containing 0.1% BSA.   Storage   The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.   Cautions   1. Please operate with lab coats and disposable gloves,for your safety.        2. This product is for research use only.  

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Interleukin 3 is a pleiotropic factor produced primarily by activated T cells that can stimulate the proliferation. At the amino acid sequence level, mature human and murine IL-3 share only 29% sequence identity. Consistent with this lack of homology, IL-3 activity is highly species-specific and human IL-3 does not show activity on murine cells. Cytokines are actively involved in the host-defense system to coordinate the functional activity and the generation of effector cells. Some of these molecules function as inflammatory mediators and hematopoietic growth factors at the same time such as IL-3. IL-3 (or multi-CSF) is known as a pleiotropic cytokine with a broad spectrum of target cells and functions. IL-3 regulates the proliferation and differentiation of normal hematopoietic progenitor cells in the early stages of hematopoiesis. And IL-3 inhibits apoptosis and promotes the autonomous growth of blast cells. Product Properties   Synonyms Hematopoietic Growth Factor, MCGF, Multipotential Colony-stimulating Factor, P-cell-stimulating Factor Source E.coli-derived Rhesus Macaque IL-3, Ala20-Gln143. AA sequence APMTQTTSLK TSWAKCSNMI DEIITHLNQP PLPSPDFNNL NEEDQTILVE KNLRRSNLEA  FSKAVKSLQN ASAIESILKN LPPCLPMATAAPTRPPIRIT NGDRNDFRRK LKFYLKTLEN EQAQ Endotoxin < 1.0 EU per μg by the LAL method. Purity > 98% by SDS-PAGE and HPLC analyses. Formulation Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4, 5 % trehalose. Applications ELISA, Kinetics (BLI), Kinetics (SPR), Immunization Dilution Dilute with sterile distilled water or aqueous buffer containing 0.1% BSA.   Storage   The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.   Cautions   1. Please operate with lab coats and disposable gloves,for your safety.        2. This product is for research use only.  

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Interleukin-5 (IL-5) is a secreted glycoprotein that belongs to the alpha -helical group of cytokines (1-3). Unlike other family members, it is present as a covalently linked antiparallel dimer. Mature rhesus IL‑5 shares 98%, 95%, 70%, 71%, 66%, 70%, 61% and 64% aa sequence identity with mature human, mangabey, mouse, rat, feline, equine, canine and bovine IL-5, respectively. IL-5 is primarily produced by CD4+ Th2 cells, but also by activated eosinophils, mast cells, EBV-transformed B cells, Reed-Sternberg cells in Hodgkin’s disease, and IL‑2‑stimulated invariant natural killer T cells (iNKT). IL-5 increases production and mobilization of eosinophils and CD34+ progenitors from the bone marrow and causes maturation of eosinophil precursors outside the bone marrow. The receptor for human IL-5, mainly expressed by eosinophils, but also found on basophils and mast cells, consists of a unique ligand-binding subunit (IL-5 R alpha ) and a shared signal-transducing subunit, beta c. IL‑5 R alpha first binds IL-5 at low affinity, then associates with preformed beta c dimers, forming a high-affinity receptor. IL-5 also binds proteoglycans, potentially enhancing its activity. Soluble forms of IL‑5 R alpha antagonize IL-5 and can be found in vivo. In humans, IL-5 primarily affects cells of the eosinophilic lineage, and promotes their differentiation, maturation, activation, migration and survival, while in mice IL‑5 also enhances Ig class switching and release from B1 cells. IL-5 also promotes differentiation of basophils and primes them for histamine and leukotriene release. Product Properties   Synonyms Eosinophil differentiation factor, TRF Source E.coli-derived Rhesus Macaque IL-5, Ile20-Ser134. AA sequence IPTEIPASAL VKETLALLST HRTLLIANET LRIPVPVHKN HQLCTEEIFQ GIGTLESQTV QGGTVERLFK NLSLIKKYIG GQKKKCGEER RRVNQFLDYL QEFLGVMNTE WIIES Endotoxin < 1.0 EU per μg by the LAL method. Purity > 98% by SDS-PAGE and HPLC analyses. Formulation Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4, 5 % trehalose. Applications ELISA, Kinetics (BLI), Kinetics (SPR), Immunization Dilution Dilute with sterile distilled water or aqueous buffer containing 0.1% BSA.   Storage   The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.   Cautions   1. Please operate with lab coats and disposable gloves,for your safety.        2. This product is for research use only.  

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Interleukin-6 (IL-6) is a pleiotropic, alpha -helical, phosphorylated and variably glycosylated cytokine that plays important roles in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression. Alternative splicing generates several isoforms with internal deletions, some of which exhibit antagonistic properties. IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL-6 R alpha) and a signal transducing subunit (gp130). IL-6 binds to IL-6 R alpha, triggering IL-6 R alpha association with gp130 and gp130 dimerization. gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM. Soluble forms of IL-6 R alpha are generated by both alternative splicing and proteolytic cleavage. In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R alpha elicit responses from gp130-expressing cells that lack cell surface IL-6 R alpha. Trans-signaling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous, while that of IL-6 R alpha is predominantly restricted to hepatocytes, monocytes, and resting lymphocytes. Soluble splice forms of gp130 block trans-signaling from IL-6/IL-6 R alpha but not from other cytokines that use gp130 as a co-receptor. IL-6, along with TNF-alpha and IL-1, function to drive the acute inflammatory response and the transition from acute inflammation to either acquired immunity or chronic inflammatory disease. Product Properties   Synonyms interleukin BSF-2; interleukin-6; MGI-2A Source E.coli-derived Rhesus Macaque IL-16, Ala28-Met212. AA sequence MAPVLPGEDS KNVAAPHSQP LTSSERIDKH IRYILDGISA LRKETCNRSN MCESSKEALA ENNLNLPKMA EKDGCFQSGF NEDTCLVKII TGLLEFEVYL EYLQNRFESS EEQARAVQMS TKVLIQFLQK KAKNLDAITT PEPTTNASLL TKLQAQNQWL QDMTTHLILR SFKEFLQSNL RALRQM Endotoxin < 1.0 EU per μg by the LAL method. Purity > 97% by SDS-PAGE and HPLC analyses. Formulation Lyophilized from a 0.2 µm filtered concentrated solution in 50 mM Tris-HCl, pH9.0, 600 mM NaCl, with 0.02 % Tween-20. Applications ELISA, Kinetics (BLI), Kinetics (SPR), Immunization Dilution Dilute with sterile distilled water or aqueous buffer containing 0.1% BSA.   Storage   The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.   Cautions   1. Please operate with lab coats and disposable gloves,for your safety.        2. This product is for research use only.  

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IL-13 is a 17 kDa immunoregulatory cytokine that plays a key role in the pathogenesis of allergic asthma and atopy. It is secreted by Th1 and Th2 CD4+ T cells, NK cells, visceral smooth muscle cells, eosinophils, mast cells, and basophils. IL-13 circulates as a monomer with two internal disulfide bonds that contribute to a bundled four alpha -helix configuration. Mature rhesus IL-13 shares 94%, 58%, and 60% amino acid sequence identity with human, mouse, and rat IL-13, respectively. Despite the low homology, it exhibits cross-species activity between human, mouse, and rat. IL-13 has diverse activities on numerous cell types. On macrophages, IL-13 suppresses the production of proinflammatory cytokines and other cytotoxic substances. On B cells, IL-13 induces immunoglobulin class switching to IgE, upregulates the expression of MHC class II, CD71, CD72, and CD23, and costimulates proliferation. IL-13 upregulates IL-6 while downregulating IL-1 and TNF-alpha production by fibroblasts and endothelial cells. IL-13 binds with low affinity to IL-13 R alpha 1, triggering IL-13 R alpha 1 association with IL-4 R alpha. This high affinity receptor complex also functions as the type 2 IL-4 receptor complex. Additionally, IL-13 binds with high affinity to IL-13 R alpha 2 which is expressed intracellularly, on the cell surface, and as a soluble molecule. Product Properties   Synonyms BHR1interleukin-13, IL13, interleukin 13, MGC116786, NC30, P600 Source E.coli-derived Rhesus Macaque IL-13, Ser19-Asn132. AA sequence SPSPVPRSTA LKELIEELVN ITQNQKAPLC NGSMVWSINL TAGVYCAALE SLINVSGCSA IEKTQRMLNG FCPHKVSAGQ FSSLRVRDTK IEVAQFVKDL LVHLKKLFRE GRFN Endotoxin < 1.0 EU per μg by the LAL method. Purity > 98% by SDS-PAGE and HPLC analyses. Formulation Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4, 3% trehalose. Applications ELISA, Kinetics (BLI), Kinetics (SPR), Immunization Dilution Dilute in 20 mM HCl to a concentration of 0.1-1.0 mg/mL.   Storage   The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.   Cautions   1. Please operate with lab coats and disposable gloves,for your safety.        2. This product is for research use only.  

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Activin A is a member of the TGF-β family and possesses a wide range of biological activities, including the regulation of cell proliferation and differentiation, as well as the promotion of neuronal survival. Elevated levels of Activin A in human colorectal cancer and postmenopausal women are associated with colorectal cancer and breast cancer, respectively. The biological activity of Activin A can be neutralized by inhibins and diffusible TGF-β antagonists such as Follistatin. Activin A binds to two forms of Activin type I receptors (ActRI-A and ActRI-B) and two forms of Activin type II receptors (ActRII-A and ActRII-B). Activins are homo- or heterodimers of different β subunits. They are produced as precursor proteins with an N-terminal propeptide that is cleaved to release the C-terminal bioactive ligand. The product is provided in the form of lyophilized powder, derived from HEK293 cells, with high activity and high purity. Recombinant human/mouse/rat Activin A is a 26.0kDa homodimer of two βA chains linked by disulfide bonds, each containing 116 amino acid residues.   Product Properties Source Human Activin A Protein is expressed from HEK293 without tag. It contains Gly311-Ser426. Accession P08467 Molecular Weight The protein has a predicted MW of 12.97 kDa. Due to glycosylation, the protein migrates to 14-15 kDa under reduced (R) condition, and 23-25 kDa under Non reducing (N) condition based on Tris-Bis PAGE result. Endotoxin Less than 0.1EU per μg by the LAL method. Purity > 95% as determined by Tris-Bis PAGE Activity Measured by its ability to inhibit proliferation of MPC-11 cells. The ED50 for this effect is < 7 ng/mL. Formulation Lyophilized from 0.22μm filtered solution in 4mM HCI. Normally 8% trehalose is added as protectant before lyophilization.   Shipping and Storage Store at -25 to -15°C, and the shelf life is 1 year after receipt of the goods. After reconstitution, store at 2 to 8°C, and the shelf life is 7 days. After reconstitution, store at -85 to -65°C, and the shelf life is 3 months. It is recommended to divide the protein equally upon first use to avoid repeated freeze-thaw cycles.   Reconstitution conditions Before opening the vial, centrifuge it first. Reconstitute with 4 mM HCl to a concentration no lower than 0.1 mg/mL.   Cautions 1. For your safety and health, please wear a lab coat and use disposable gloves when handling.  2.This product is intended for research purposes only.

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Description Granulocyte-macrophage colony-stimulating factor (GM-CSF), is a monomer glycoprotein that stimulates the growth and differentiation of hematopoietic progenitor cells of different lineages, including granulocytes, macrophages, eosinophils, and erythrocytes.    Product Properties  Synonyms Granulocyte-Macrophage Colony-Stimulating Factor, GM-CSF, CSF-2, MGI-1GM, Pluripoietin-α Uniprot No. P04141 Source Recombinant Human GM-CSF Protein is expressed from HEK293 Cells with His tag at the N-terminal. It contains Ala18-Glu144. Molecular Weight The protein has a predicted MW of 16.41 kDa. And it migrates as 20-30 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. Purity > 95% as determined by SDS-PAGE. Endotoxin <0.01 EU per 1μg of the protein by the LAL method. Formulation Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Reconstitution Centrifuge tubes before opening. Dissolve lyophilized protein with sterile water to ensure the concentration is greater than 100 ug/mL.   Shipping and Storage The product should be stored at -25~-15℃ for 1 year from date of receipt. 2-7 days, 2 ~8 °C under sterile conditions after reconstitution. 3 months, -25~-15℃ under sterile conditions after reconstitution. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.    Cautions 1.Please operate with lab coats and disposable gloves,for your safety. 2.This product is for research use only.

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Description IL-6 is similar to other interleukins in that it has a compact globular folding structure and is held in place by two disulfide bonds. The cysteine involved in forming these two disulfide bonds is highly conserved. IL-6, expressed by a variety of cells, regulates cell growth and differentiation in various tissues and is an important pro-inflammatory and immunomodulatory cytokine.    Product Properties  Synonyms Interleukin-6,BSF2,HSF,IFNB2 Uniprot No. P05231 Source Recombinant Human IL-6 Protein is expressed from HEK293 Cells with His tag at the C-terminal. It contains Val30-Met212. Molecular Weight The protein has a predicted MW of 22.75 kDa. And it migrates as 25-30 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. Purity > 95% as determined by SDS-PAGE. Biological Activity The ED50 as determined by a cell proliferation assay using human TF-1 cells is 0.23-0.48 ng/mL, corresponding to a specific activity of > 2.0 × 106 IU/mg. Endotoxin < 1 EU per 1μg of the protein by the LAL method. Formulation Lyophilized from a 0.2 μm filtered concentrated solution in PBS. Reconstitution Centrifuge tubes before opening. Dissolve lyophilized protein with PBS. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.     Shipping and Storage The product should be stored at -25~-15℃ for 1 year from date of receipt. 2-7 days, 2 ~8 °C under sterile conditions after reconstitution. 3 months, -25~-15℃ under sterile conditions after reconstitution. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.    Cautions 1.Please operate with lab coats and disposable gloves,for your safety. 2.This product is for research use only.

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Description GDF-15,Placental Transforming Growth Factor beta, Prostate-derived Factor, and Placental Bone Morphogenetic Protein, is a divergent member of the Transforming Growth Factor beta (TGF-beta ) superfamily   Product Properties Synonyms Growth Differentiation Factor 15,MIC-1 Uniprot No. Q99988 Source Human GDF-15 Protein is expressed from E. coli.It contains Ala197-Ile308 Molecular Weight Predicted molecular mass of approximately 12 kDa in SDS-PAGE under reducing condition. Biological Activity Measured by its binding ability in a functional ELISA. When rHuGDF-15/MIC-1 is used at 0.5 µg/mL, the concentration of Recombinant Human Activin RIB/ALK-4 Fc Chimera that produces 50% of the optimal binding response is approximately 0.5-3 μg/mL. Purity > 95% as determined by SDS-PAGE. Endotoxin <0.1 EU per 1μg of the protein by the LAL method. Formulation Lyophilized from 0.2 µm filtered concentrated solution in 4 mM HCl. Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile 4 mM HCl to a concentration of 0.5 mg/mL. Further dilutions should be made in appropriately buffered solutions.   Shipping and Storage The product should be stored at -25~-15℃ for 1 year from date of receipt. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.   Cautions 1.Please operate with lab coats and disposable gloves,for your safety. 2.This product is for research use only.

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Description Interleukin 5 (IL-5) is a member of the hematopoietic Th2 cytokine family and consists of 115 amino acids. Unlike other members of the cytokine family (IL-3 and GM-CSF), this glycoprotein is activated as a homologous dimer. IL-5 is secreted by Th2 cells and mast cells. Its biological activity on B cells and other cell types suggests that IL-5 is functionally important for Th2 cell activity. By binding to its receptor, IL-5 stimulates B cell growth and enhances immunoglobulin secretion. It is also a key factor in the activation of eosinophils. IL-5 has been associated with a variety of diseases, including allergic rhinitis and asthma, with substantial increases seen in circulating tissue, tracheal tissue, and a decrease in sputum eosinophils.   Product Properties Synonyms TRF,Interleukin-5 Uniprot No. P05113-1 Source Recombinant Human IL-5 Protein is expressed from HEK293 Cells with His tag at the N-terminal and Fc tag at the C-terminal. It contains Ile20-Ser134. Molecular Weight The protein has a predicted MW of 45.33 kDa. Purity > 95% as determined by SDS-PAGE. Endotoxin <0.1 EU per 1μg of the protein by the LAL method. Formulation Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Reconstitution Centrifuge tubes before opening. The concentration of protein solution used for lyophilization is generally 1mg/mL. Dissolve lyophilized protein with sterile water to ensure the concentration is greater than 100 ug/mL.   Shipping and Storage The product should be stored at -25~-15℃ for 1 year from date of receipt. 2-7 days, 2 ~8 °C under sterile conditions after reconstitution. 3 months, -25~-15℃ under sterile conditions after reconstitution. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.   Cautions 1.Please operate with lab coats and disposable gloves,for your safety. 2.This product is for research use only.

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Description IL-10 is an anti-inflammatory cytokine. IL-10 is produced primarily by monocytes, but also secreted by T cells, macrophages, mast cells, and other cell lines. IL-10 can inhibit the secretion of cytokines such as IFN- γ, IL-2, IL-3, TNF and GM-CSF, which plays an important role in the fight against human transitional immunity. In addition, IL-10 may play a role in the clinical inhibition of organ transplantation rejection due to its immunosuppressive properties.   Product Properties Synonyms CSIF;IL10 Uniprot No. P05231 Source Recombinant Human IL-10 Protein is expressed from HEK293 Cells with His tag at the C-terminal. It contains Ser19-Asn178. Molecular Weight The protein has a predicted MW of 19.79 kDa. Purity > 95% as determined by SDS-PAGE. Endotoxin <0.1 EU per 1μg of the protein by the LAL method. Formulation Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Reconstitution Centrifuge tubes before opening. The concentration of protein solution used for lyophilization is generally 1mg/mL. Dissolve lyophilized protein with sterile water to ensure the concentration is greater than 100 ug/mL.   Shipping and Storage The product should be stored at -25~-15℃ for 1 year from date of receipt. 2-7 days, 2 ~8 °C under sterile conditions after reconstitution. 3 months, -25~-15℃ under sterile conditions after reconstitution. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.   Cautions 1.Please operate with lab coats and disposable gloves,for your safety. 2.This product is for research use only.

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Description NGF was discovered as a molecule that promoted the survival and differentiation of sympathetic and sensory neurons in the peripheral nervous system.NGF is the first member discovered in the Neurotrophin family, which includes brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3), and neurotrophin-4 (NT-4).   Product Properties Synonyms beta Nerve Growth Factor; Ngfb Uniprot No. NP_001106168.1 Source Recombinant Mouse NGF Protein is expressed from CHO Stable Cells Cells with an initial Met at the C-terminus. It contains Ser 122-Gly 241. Molecular Weight The recombinant mouse NGF has a calculated molecular mass of 13.5 kDa as estimated in SDS-PAGE under reducing conditions. Biological Activity Measured in a cell proliferation assay using  TF-1  human  erythroleukemic  cells.  The  ED50 for this effect is 1-6 ng/mL. Purity > 95% as determined by SDS-PAGE. Endotoxin <1 EU per 1μg of the protein by the LAL method. Formulation Lyophilized from sterile 150mM NaCl, 50mM NaAc, pH 5.5.Normally 5% - 8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring  the  contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.   Shipping and Storage The product should be stored at -25~-15℃ for 1 year from date of receipt. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.   Cautions 1.Please operate with lab coats and disposable gloves,for your safety. 2.This product is for research use only.

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Description Bone morphogenetic proteins (BMPs) constitute a subfamily of structurally related signaling proteins within the TGF-β superfamily. Members of this superfamily are widely distributed throughout the body and are involved in various physiological processes both prenatally and postnatally. Like BMP-7, BMP-4 is also involved in the development and maintenance of bone and cartilage. Reduced expression of BMP4 is associated with many skeletal diseases, including the hereditary condition progressive diaphyseal dysplasia. Mature mouse and human BMP-4 have 98% amino acid (aa) homology. This product features high activity, high purity, and low endotoxin levels. It has been validated and tested for bioactivity, endotoxin levels, and SDS-PAGE to ensure the consistency of biological activity and batch-to-batch uniformity. Our product is provided in a carrier-free form, making it highly suitable for research and production of cell culture, ELISA, or immunoblotting standards.   Product Properties Synonyms Bone Morphogenetic Protein 4;BMP4;BMP2B; BMP-2B; BMP2B1; Uniprot No. P21275 Source E.coil-derived Mouse BMP-4 protein,Lys303-Arg408 with His tag at the C-terminus Amino Acid MKKNKNCRRHSLYVDFSDVGWND WIVAPPGYQAFYCHGDCPFPLADHL NSTNHAIVQTLVNSVNSSIPKACCVP TELSAISMLYLDEYDKVVLKNYQEMV VEGCGCR Molecular Weight Approximately 14 kDa. Biological Activity Measure by its ability to induce alkaline phosphatase production by ATDC5 cells. The ED50 for this effect is 10 ng/mL. The specific activity of recombinant mouse BMP-4 is > 1 x 100000 IU/mg. Purity > 98% as determined by SDS-PAGE. Endotoxin <0.1 EU per 1μg of the protein by the LAL method. Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 1×PBS, pH4.5. Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring  the  contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.   Shipping and Storage Ice pack shipping. Store at -20°C with a one-year shelf life. Recommended to aliquot and freeze upon first use to avoid repeated freezing and thawing.   Cautions 1.Please operate with lab coats and disposable gloves,for your safety. 2.This product is for research use only.   Usage 1. It is recommended to reconstitute the lyophilized powder with sterile water, ensuring that the solution concentration is not less than 100 μg/mL, and let it stand for at least 20 minutes to fully dissolve. 2. After reconstitution, the solution can be further diluted and aliquoted, stored at 2-8°C with a shelf life of 1 month, and at -20°C with a shelf life of 3 months; avoid repeated freezing and thawing. 3. When further diluting and aliquoting the reconstituted solution, a certain amount of carrier protein should be added (0.1% BSA, 10% FBS, or 5% HSA). For experiments that require serum-free conditions, it can be replaced with a 5% trehalose solution as the carrier.

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Description CXCL11, also known as I-TAC, SCYB9B, H174 and beta -R1, is a non-ELR CXC chemokine. CXCL11 cDNA encodes a 94 amino acid (aa) residue precursor protein with a 21 aa residue putative signal sequence, which is cleaved to form the mature 73 aa residue protein. CXCL11 shares 36% and 37% amino acid sequence homology with IP-10 and MIG (two other known human non-ELR CXC chemokines), respectively. CXCL11 is expressed at low levels in normal tissues including thymus, spleen and pancreas. The expression of CXCL11 mRNA is radically up regulated in IFN-gamma and IL-1 stimulated astrocytes. Moderate increase in expression is also observed in stimulated monocytes. CXCL11 has potent chemoattractant activity for IL-2 activated T cells and transfected cell lines expressing CXCR3, but not freshly isolated T cells, neutrophils or monocytes.   Product Properties Synonyms Beta-R1, H174, IP-9, Small-inducible Cytokine B11 Uniprot No. O14625; Gene ID 6373 Source E.coli-derived Human CXCL11,Phe22-Phe94. Amino Acid FPMFKRGRCL CIGPGVKAVK VADIEKASIM YPSNNCDKIE VIITLKENKG QRCLNPKSKQARLIIKKVER KNF Molecular Weight Approximately 8.3 kDa Biological Activity Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human IL-2 activated human T-lymphocytes is in a concentration range of 0.1-10 ng/mL. Purity > 97% by SDS-PAGE and HPLC analyses. Endotoxin < 1 EU per 1μg of the protein by the LAL method. Formulation Lyophilized from a 0.2 µm filtered concentrated solution in 20 mM PB, pH 7.4, 100 mM NaCl. Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.   Shipping and Storage The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.   Cautions 1. Avoid repeated freeze-thaw cycle. 2. For your safety and health, please wear lab coats and disposable gloves for operation. 3. For research use only.   Usage 1. It is recommended to reconstitute the lyophilized powder with sterile water, ensuring that the solution concentration is not less than 100 μg/mL, and let it stand for at least 20 minutes to fully dissolve. 2. After reconstitution, the solution can be further diluted and aliquoted, stored at 2-8°C with a shelf life of 1 month, and at -20°C with a shelf life of 3 months; avoid repeated freezing and thawing. 3. When further diluting and aliquoting the reconstituted solution, a certain amount of carrier protein should be added (0.1% BSA, 10% FBS, or 5% HSA). For experiments that require serum-free conditions, it can be replaced with a 5% trehalose solution as the carrier.

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VEGF 165, also known as Vascular Endothelial Growth Factor 165, is a protein that plays a critical role in angiogenesis, the process of forming new blood vessels from pre-existing ones. It belongs to the vascular endothelial growth factor family and is one of the most well-studied isoforms of VEGF.    Product Properties Synonyms   VEGF-165；VEGF; VEGFA; MVCD1;  VPF; RP1-261G23.1;   Uniprot No. P15692-4 Source Recombinant Human VEGF165 Protein is expressed from HEK293 without tag. It contains Ala27-Arg191 Molecular Weight The protein has a predicted MW of 19.2 kDa. Due to glycosylation, the protein migrates to 20-30 kDa based on Tris-Bis PAGE result. Biological Activity ED50 < 3 ng/ml, measured in a cell proliferation assay using HUVEC cells. Purity > 95% by SDS-PAGE and HPLC Endotoxin <1.0 EU per 1μg of the protein by the LAL method. Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Reconstitution It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.   Shipping and Storage The product should be stored at -25~-15℃ for 1 year from date of receipt. 2-7 days, 2 ~8 °C under sterile conditions after reconstitution. 3 -6 months, -85~-65℃ under sterile conditions after reconstitution. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.   Cautions 1.Please operate with lab coats and disposable gloves,for your safety. 2.This product is for research use only.

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Product Properties Synonyms   Wingless-type MMTV Integration Site Family, Member 5a; wingless-type MMTV integration site family, member 5A; Wnt5a   Source HEK293 Cells, Gln38-Lys380 with a His tag at the C-terminal UniProt P41221 Molecular Weight The protein has a predicted MW of 40kDa. The protein migrates as 60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation Purity > 90% by SDS-PAGE. Endotoxin < 1.0 EU per 1μg of the protein by the LAL method. Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 5% trehalose and 0.01 % Tween 80 were added as protectant before lyophilization Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.   Shipping and Storage The product should be stored at -85~-65℃ for 1 year. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.   Cautions 1.Please operate with lab coats and disposable gloves,for your safety. 2.This product is for research use only.

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The platelet-derived growth factor (PDGF) family of heparin-binding growth factors consists of five known members, denoted PDGF-AA, PDGF-BB, PDGF-AB, PDGF-CC and PDGF-DD.The PDGFs interact with two related protein tyrosine kinase receptors, PDGFR-α and PDGFR-β, and are potent mitogens for a variety of cell types. They play an important role in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubules' epithelial cell development.   Product Properties Synonyms   PDGFCC; PDGF-CC   Source E.coli Molecular Weight Approximately 27 kDa, a disulfide-linked homodimer of two 117 amino acid, C-terminal polyhistidine tagged proteins. AA Sequence Accession :NP_057289 Val235-Gly345,  with an N-terminal Met and 6-His tag; Biological Activity Measured in a cell proliferation assay using NR6R3T3 mouse fibroblast cells. The ED50 for this effect is 70-350 ng/mL. Purity > 97% by SDS-PAGE. Endotoxin <0.1EU per 1μg of the protein by the LAL method. Formulation Lyophilized from 0.2 µm filtered concentrated solution in 30 % Acetonitrile and 0.1 % TFA. Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 4 mM HCl to a concentration of 0.1 mg/mL. Further dilutions should be made in appropriately buffered solutions.   Shipping and Storage The product should be stored at -25~-15℃ for 1 year from date of receipt. 1 month, 2 ~8 °C under sterile conditions after reconstitution. 3 months, -25~-15℃ under sterile conditions after reconstitution. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.   Cautions 1.Please operate with lab coats and disposable gloves,for your safety. 2.This product is for research use only.

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Human hepatocyte growth factor (HGF) protein is a naturally occurring protein that plays a key role in cell growth and repair. It is primarily produced and released by cells in the liver, and it acts as a signaling molecule in various tissues throughout the body. human HGF protein is a crucial protein that regulates cell growth and repair processes. Its role in promoting tissue regeneration and maintaining organ health makes it a promising candidate for medical interventions and regenerative therapies.   Product Properties Synonyms   Scatter Factor (SF), Hepatopoietin (HPTA)   Uniprot No.  P14210 Source HEK293 cells -derived human HGF protein,Gln32-Ser728, with N-terminal Flag Tag. Molecular Weight 90~100 kDa (alpha & beta chain), 66~70 kDa (alpha chain),30~34 kDa (beta chain), on SDS-PAGE under reducing conditions. Purity > 50% as determined by SDS-PAGE. Endotoxin < 1 EU per 1μg of the protein by the LAL method. Formulation Liquid in PBS，the concentration is 0.04 mg/ml.     Shipping and Storage The product should be stored at -25~-15℃ for 1 year from date of receipt. Cautions 1.Please operate with lab coats and disposable gloves,for your safety. 2.This product is for research use only.

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Human Wnt-3a protein is a signaling protein that belongs to the Wnt family. It plays a crucial role in various developmental processes, including cell fate determination, tissue patterning, and organogenesis. Wnt-3a protein is secreted by cells and acts as a ligand, binding to specific receptors on the surface of target cells to activate intracellular signaling pathways.   Product Properties Synonyms   Wingless-type MMTV Integration Site Family, Member 3a   Uniprot No.  P56704 Source HEK293 cells-derived human Wnt-3a protein, Ser19-Lys352, with C-terminal His tag. Molecular Weight The protein has a predicted MW of 39.4 kDa. And it migrates as 43 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. Purity > 50% as determined by SDS-PAGE. Endotoxin < 1 EU per 1μg of the protein by the LAL method. Formulation Liquid in PBS，the concentration is 0.015 mg/ml.   Shipping and Storage The product should be stored at -25~-15℃ for 1 year from date of receipt. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles. Cautions 1.Please operate with lab coats and disposable gloves, for your safety. 2.This product is for research use only.

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Human RSPO1 protein, also known as R-Spondin 1, is a naturally occurring protein in the human body that plays a significant role in the Wnt signaling pathway. This pathway regulates various cellular functions, including cell growth, differentiation, and tissue regeneration. RSPO1 acts as an agonist, meaning it enhances the activity of Wnt proteins by binding to their receptors and activating them. This activation leads to downstream signaling events that impact cell behavior and tissue development.   Product Properties Synonyms   R-Spondin 1；Roof Plate-specific Spondin 1   Uniprot No.  Q2MKA7 Source E.coli-derived human RSPO1 protein, Arg21-Ala263，with N-terminal His tag. Molecular Weight Approximately 27.6 kDa. Purity > 50% as determined by SDS-PAGE. Endotoxin < 1 EU per 1μg of the protein by the LAL method. Formulation Liquid in 20 mM Tris，1 M NaCl，pH8.0，the concentration is 0.05 mg/ml.   Shipping and Storage The product should be stored at -25~-15℃ for 1 year from date of receipt. Cautions 1.Please operate with lab coats and disposable gloves, for your safety. 2.This product is for research use only.

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CXC chemokine ligand 16 (CXCL16) is a type I membrane protein containing a non-ELR motif-containing CXC chemokine domain in its extracellular region. Functional CXCL16 can be shed from the cell surface as an approximately 35 kDa soluble protein.   Product Properties Synonyms   CXCLG16, SR-PSOX，SRPSOX   Uniprot No.  Q9H2A7-1 Source Recombinant Human CXCL16 Protein is expressed from E.coli Cells with tag free. It contains Asn 30 - Thr 205. Molecular Weight The protein has a predicted MW of 19.5kDa. Purity > 95% as determined by SDS-PAGE. Endotoxin <1 EU per 1μg of the protein by the LAL method. Formulation Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Reconstitution Centrifuge tubes before opening. The concentration of protein solution used for lyophilization is generally 1mg/mL. Dissolve lyophilized protein with sterile water to ensure the concentration is greater than 100 ug/mL.   Shipping and Storage The product should be stored at -25~-15℃ for 1 year from date of receipt. 2-7 days, 2 ~8 °C under sterile conditions after reconstitution. 3 months, -25~-15℃ under sterile conditions after reconstitution. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles. Cautions 1.Please operate with lab coats and disposable gloves,for your safety.

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Laminin 521 is a defined matrix for feeder-free culture of human embryonic (hES) and induced pluripotent (hiPS) stem cells. When used in combination with defined maintenance media, Laminin 521 reliably facilitates self-renewal of human hES and hiPS cells without spontaneous differentiation.   Product Properties Synonyms   LN521，LAMB2,LN-11   Source Mammalian cells Purity > 95% Endotoxin <1 EU/ ml TSE/BSE Animal-Free Mycoplasma Negative Concentration 0.1 mg/ml Formulation Clear colorless, buffered solution with a pH of 7.4 with PBS. Form Liquid   Shipping and Storage This product should be stored at -85~-65℃ for 2 years from date of receipt. 3 months at 2-8°C after thaw. Cautions 1. This product is for research use only.  2. Please operate with lab coats and disposable gloves，for your safety.

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Product Properties Synonyms   EPO; Erythropoietin; EP; MVCD2; Epoetin   Source HEK293 Cells Molecular Weight The recombinant mouse Epo predicts a molecular mass of 18.6 kDa. AA Sequence NP_031968.1 ((Met1-Arg192) Biological Activity Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 2-10 ng/mL. Purity > 95% by SDS-PAGE. Endotoxin <1.0 EU per 1μg of the protein by the LAL method. Formulation Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C.   Shipping and Storage The product should be stored at -25~-15℃ for 1 year from date of receipt. 1 month, 2 ~8 °C under sterile conditions after reconstitution. 3 months, -25~-15℃ under sterile conditions after reconstitution. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles. Cautions 1.Please operate with lab coats and disposable gloves,for your safety. 2. This product is for research use only.

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IL-12 is a cytokine protein that plays a key role in the immune system's response to infection and cancer. It stimulates the activation and proliferation of T cells and natural killer cells, and also stimulates the production of interferon-gamma, an important immune signaling molecule. IL-12 has been studied for its potential use in cancer immunotherapy and vaccine development.   Product Properties   Synonyms Interleukin-12, IL-12, NKSF, CTL Maturation Factor (TCMF), Cytotoxic Lymphocyte Maturation Factor (CLMF), TSF Source Recombinant Human IL-12 Protein is expressed from HEK293 Cells with His tag at the N-terminal. IL-12A contains Arg23-Ser219. IL-12B contains Ile23-Ser328. Endotoxin < 1.0 EU per μg by the LAL method. Purity > 95% by SDS-PAGE and HPLC analyses. Formulation Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. Applications ELISA, Kinetics (BLI), Kinetics (SPR), Immunization Dilution Dilute with PBS.   Storage   The products are shipped with ice pack and can be stored at -25~-15℃ for 1 year. 2-7 days, 2~8 °C under sterile conditions after reconstitution.&nbsp; 3 months,&nbsp; -25~-15℃ under sterile conditions after reconstitution. Recommend to aliquot the protein into smaller quantities when first used&nbsp;and avoid repeated freeze-thaw cycles. Cautions   1. Please operate with lab coats and disposable gloves,for your safety.        2. This product is for research use only.  

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GM-CSF is a powerful growth and differentiation factor which acts on hematopoietic progenitor cells and also activates differentiated granulocytes and macrophages.   Product Properties   Synonyms Granulocyte-Macrophage Colony-Stimulating Factor, GM-CSF, CSF-2, MGI-1GM, Pluripoietin-α Source Recombinant Mouse GM-CSF Protein is expressed from Yeast with N-HIS. It contains Ala18-Lys141. Endotoxin < 1.0 EU per μg by the LAL method. Purity > 95% by SDS-PAGE and HPLC analyses. Formulation Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4,with 1%BSA. Applications ELISA, Kinetics (BLI), Kinetics (SPR), Immunization Dilution Dilute with PBS.   Storage   The products are shipped with ice pack and can be stored at -25~-15℃ for 1 year. 2-7 days, 2~8 °C under sterile conditions after reconstitution.&nbsp; 3 months,&nbsp; -25~-15℃ under sterile conditions after reconstitution. Recommend to aliquot the protein into smaller quantities when first used&nbsp;and avoid repeated freeze-thaw cycles. Cautions   1. Please operate with lab coats and disposable gloves,for your safety.        2. This product is for research use only.  

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IL-23 is a proinflammatory, heterodimeric protein composed of two subunits: a unique p19 subunit, and a p40 subunit that is shared with IL-12. IL-23 is secreted by activated dendritic cells and macrophages, and signals though a receptor comprised of IL-23R complexed with IL-12Rβ1. IL-23 has been shown to enhance proliferation of memory T cells.   Product Properties   Synonyms Interleukin-23 Source Sf 21 (baculovirus) Endotoxin < 1.0 EU per μg by the LAL method. Purity > 95% by SDS-PAGE and HPLC analyses. Formulation Lyophilized from 0.2 μm filtered concentrated sterile solution in 20 mM MES, pH 6.0, 500 mM NaCl and 1 mM EDTA. Applications ELISA, Kinetics (BLI), Kinetics (SPR), Immunization Dilution Dilute with 20 mM MES, pH 6.0, 500 mM NaCl and 1 mM EDTA.   Storage    The products are shipped with ice pack and can be stored at -25~-15℃ for 1 year. 1 month, 2 ~8 °C under sterile conditions after reconstitution. 3 months, -25~-15℃ under sterile conditions after reconstitution. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.   Cautions   1. Please operate with lab coats and disposable gloves,for your safety.        2. This product is for research use only.  

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IL-23 is a proinflammatory, heterodimeric protein composed of two subunits: a unique p19 subunit, and a p40 subunit that is shared with IL-12. IL-23 is secreted by activated dendritic cells and macrophages, and signals though a receptor comprised of IL-23R complexed with IL-12Rβ1. IL-23 has been shown to enhance proliferation of memory T cells.   Product Properties   Synonyms Interleukin-23 Source HEK293 Cells Endotoxin < 1.0 EU per μg by the LAL method. Purity > 95% by SDS-PAGE and HPLC analyses. Formulation Lyophilized from sterile PBS, pH7.4. Normally 5% - 8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Applications ELISA, Kinetics (BLI), Kinetics (SPR), Immunization Dilution Dilute with PBS.   Storage    The products are shipped with ice pack and can be stored at -25~-15℃ for 1 year. 1 month, 2 ~8 °C under sterile conditions after reconstitution. 3 months, -25~-15℃ under sterile conditions after reconstitution. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.   Cautions   1. Please operate with lab coats and disposable gloves,for your safety.        2. This product is for research use only.  

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Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-3 (stromelysin-1), can degrade a broad range of substrates including collagen alpha chains, aggrecan, laminin, fibronectin, elastin, casein, alpha -1 antitrypsin, myelin basic protein, IL-1 beta, IGFBP-3, pro MMP-1, pro MMP-7, pro MMP-8, pro MMP-9 and pro MMP-13. The MMP-3 substrate repertoire extends beyond extracellular matrix proteins and implicates MMP-3 in roles other than direct tissue remodelling, for instance, enzyme cascades and cytokine regulation   Product Properties   Synonyms Matrix Metalloproteinase-3, Stromelysin-1, SL-1, Transin-1 Source Recombinant Human MMP-3 Protein is expressed from E.coli with His tag at the C-terminal. It contains Arg101-Cys477. Endotoxin < 1.0 EU per μg by the LAL method. Purity > 95% by SDS-PAGE and HPLC analyses. Formulation Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Applications ELISA, Kinetics (BLI), Kinetics (SPR), Immunization Dilution Dilute with PBS.   Storage    The products are shipped with ice pack and can be stored at -25~-15℃ for 1 year. 2-7 days, 2 ~8 °C under sterile conditions after reconstitution. 3 months, -25~-15℃ under sterile conditions after reconstitution. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.   Cautions   1. Please operate with lab coats and disposable gloves,for your safety.        2. This product is for research use only.  

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IL-4 is a protein that regulates the immune response by promoting the growth and differentiation of B cells. It also plays a role in the development of allergic reactions and asthma. IL-4 is produced by a variety of cells, including T cells and mast cells.   Product Properties   Synonyms Interleukin-4, IL-4, BSF-1, BCDF, IL4E12, Pitrakinra Source Human IL-4 Protein is expressed from HEK293 Cells with His tag at the C-terminal. It contains His25-Ser153. Endotoxin < 0.01 EU per μg by the LAL method. Purity > 95% by SDS-PAGE analyses. Formulation Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Applications ELISA, Kinetics (BLI), Kinetics (SPR), Immunization Dilution Dilute with PBS.   Storage    The products are shipped with ice pack and can be stored at -25~-15℃ for 1 year. 2-7 days, 2 ~8 °C under sterile conditions after reconstitution. 3 months, -25~-15℃ under sterile conditions after reconstitution. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.   Cautions   1. Please operate with lab coats and disposable gloves,for your safety.        2. This product is for research use only.  

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Fms-like tyrosine kinase 3 ligand (Flt-3 Ligand) is a cytokine that promotes differentiation of a variety of hematopoietic cells. Flt3L can increase the number of immune cells by activating hematopoietic progenitor cells.   Product Properties   Synonyms FLT3LG;FL;FLT3L Source Recombinant Human Flt3-Ligand Protein is expressed from HEK293 Cells with His tag at the N-terminal. It contains Thr27-Pro184. Endotoxin < 0.1 EU per μg by the LAL method. Purity > 95% by SDS-PAGE analyses. Formulation Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Applications ELISA, Kinetics (BLI), Kinetics (SPR), Immunization Dilution Dilute with PBS.   Storage    The products are shipped with ice pack and can be stored at -25~-15℃ for 1 year. 2-7 days, 2 ~8 °C under sterile conditions after reconstitution. 3 months, -25~-15℃ under sterile conditions after reconstitution. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.   Cautions   1. Please operate with lab coats and disposable gloves,for your safety.        2. This product is for research use only.  

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CNTF protein is a neurotrophic factor that promotes neuronal survival and differentiation. It is involved in the regulation of various cellular processes, such as inflammation, metabolism, and immune response. CNTF has potential therapeutic applications for the treatment of neurodegenerative diseases.   Product Properties   Synonyms HCNTF,ciliary neurotrophic factor Source Recombinant Human CNTF Protein is expressed from HEK293 Cells with His tag at the N-terminal. It contains Met1-Met200. Endotoxin < 0.01 EU per μg by the LAL method. Purity > 95% by SDS-PAGE analyses. Formulation Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Applications ELISA, Kinetics (BLI), Kinetics (SPR), Immunization Dilution Dilute with PBS.   Storage    The products are shipped with ice pack and can be stored at -25~-15℃ for 1 year. 2-7 days, 2 ~8 °C under sterile conditions after reconstitution. 3 months, -25~-15℃ under sterile conditions after reconstitution. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.   Cautions   1. Please operate with lab coats and disposable gloves,for your safety.        2. This product is for research use only.  

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Monocyte colony-stimulating factor (M-CSF) is a macrophage lineage-specific growth factor. M-CSF Induces vascular endothelial growth factor production and angiogenic activity from human monocytes. Whatmore, M-CSF and GM-CSF are mediators involved in regulating the numbers and function of macrophage lineage populations and have been shown to contribute to macrophage heterogeneity.   Product Properties   Synonyms CSF-1, MGI-IM, MCSF, M-CSF, MGC31930 Source Recombinant Mouse M-CSF is expressed from Yeast with N-His. It contains Lys33-Glu262. Endotoxin < 1.0 EU per μg by the LAL method. Purity > 95% by SDS-PAGE analyses. Formulation Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4), with 1%BSA. Applications ELISA, Kinetics (BLI), Kinetics (SPR), Immunization Dilution Dilute with PBS,with 1%BSA.   Storage    The products are shipped with ice pack and can be stored at -25~-15℃ for 1 year. 2-7 days, 2 ~8 °C under sterile conditions after reconstitution. 3 months, -25~-15℃ under sterile conditions after reconstitution. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.   Cautions   1. Please operate with lab coats and disposable gloves,for your safety.        2. This product is for research use only.  

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Activin A, a homodimer of activin βA subunits, is a member of the transforming growth factor (TGF)-β super-family. Activin A is produced by the placenta during human pregnancy, has been recognized as a multifunctional cytokine expressed in a wide range of tissues and cells with roles in regulation of wound repair, cell differentiation, apoptosis, embryogenesis, and inflammation.   Product Properties   Synonyms Activin A, activin AB alpha polypeptide, inhibin beta A chain Source Human/Murine/Rat Activin A Protein is expressed from CHO cell.It contains Gly311 - Ser426. Endotoxin < 0.01 EU per μg by the LAL method. Purity > 95% by SDS-PAGE analyses. Formulation Lyophilized from 0.2 μm filtered concentrated solution in 30% acetonitrile and 0.1 % TFA. Applications ELISA, Kinetics (BLI), Kinetics (SPR), Immunization Dilution Dilute with sterile 4 mM HCl to a concentration of 0.1-0.5 mg/ml.   Storage    The products are shipped with ice pack and can be stored at -25~-15℃ for 1 year. 1 month, 2 ~8 °C under sterile conditions after reconstitution. 3 months, -25~-15℃ under sterile conditions after reconstitution. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.   Cautions   1. Please operate with lab coats and disposable gloves,for your safety.        2. This product is for research use only.  

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Interleukin-18 (IL-18) is a potent pro-inflammatory cytokine that can induce the production of interferon-gamma in Th1 cells, NK cells, and activated macrophages, especially in the presence of IL-12. IL-18 also plays a role in developmental regulation. This product features high activity, high purity, and low endotoxin levels. The product is verified and tested for biological activity, endotoxin levels, and through SDS-PAGE to ensure the biological activity and batch-to-batch consistency; our product is provided in a carrier-free form, making it highly suitable for research and production in cell culture, ELISA, or as a standard for immunoblotting. Product Properties Aliases Interleukin 18; Interleukin-18 Uniprot No. P70380 Expression Range and System E.coli-derived Mouse IL-18 protein, Asn36-Ser192 with a His tag at the C-terminus. Molecular Weight Approximately 18 kDa. Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity Greater than 98% as determined by SDS-PAGE. Activity Measured by its ability to induce IFN gamma secretion in KG-1 cells. The ED50 for this effect is 0.5 μg/mL. Endotoxin Less than 0.1 EU per 1μg of the protein by the LAL method. Formulation Lyophilized (freeze-dried) from a solution containing 1×PBS, pH 8.0.   Usage Instructions 1.It is recommended to reconstitute the lyophilized powder with sterile water to a concentration of no less than 100 μg/mL and let it stand for at least 20 minutes to ensure complete dissolution. 2.After reconstitution, the solution can be further diluted and aliquoted for storage at 2-8°C with a shelf life of 1 month, or at -20°C with a shelf life of 3 months; avoid repeated freeze-thaw cycles. 3.When further diluting and aliquoting the reconstituted solution, it is necessary to add a certain amount of carrier protein (0.1% BSA, 10% FBS, or 5% HSA). For serum-free experimental requirements, it can be replaced with a 5% trehalose solution as the carrier.   Shipping and Storage Ice Pack Shipping:Store at -20°C, with a one-year shelf life. Recommendation for Initial Use: It is advised to aliquot and freeze the product for storage upon the first use to prevent repeated freezing and thawing.   Cautions 1. For your safety and health, please wear a lab coat and use disposable gloves when handling. 2.This product is for research use only.

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Vascular endothelial growth factor C (VEGF-C) and VEGF-D constitute a subfamily of the angiogenic VEGF angiogenic factors. VEGF-C is synthesized as a 58 kDa molecule that consists of a VEGF homolgy domain (VHD) flanked by N- and C-terminal propeptides. The proprotein undergoes covalent homodimerization and stepwise proteolytic processing to generate ligands with increasing affinity for VEGF R3/Flt-4. Fully processed VEGF-C containing just the 21 kDa VHD can additionally bind and activate VEGF R2/KDR/Flk-1. Fully processed human VEGF-C shares 98% amino acid sequence identity with mouse and rat VEGF-C. VEGF-C interactions with VEGF R3 are critical for lymphangiogenesis. VEGF-C and VEGF R3 are usually co-expressed at sites with lymphatic vessel sprouting, in the embryo, and in various pathological conditions. Over-expression of VEGF-C in tumor cells induces tumoral lymphatic hyperplasia, resulting in enhanced lymph flow and metastasis to regional lymph nodes. Product Properties Synonyms Flt4 ligand; Flt4-L; vascular endothelial growth factor C; Vascular endothelial growth factor-related protein; VEGFC; VEGF-C; VRPFLT4 ligand DHM Accession P49767 Source HEK293 Cells-derived human VEGF-C, Thr103-Arg227 with a C-terminal polyhistidine tag.  Molecular Weight The recombinant mature form of human VEGFC consists of 136 amino acids and has a predicted molecular mass of 15.5 kDa. In SDS-PAGE under reducing conditions, it migrates with an apparent molecular mass of 22.5 kDa due to glycosylation. Tag His Purity > 95% by SDS-PAGE. Biological Activity 1. Measured by its binding ability in a functional ELISA. Immobilized VEGF C-his at2μg/mL(100 μL/well) can bind VEGFR3 hFc, the EC50 of VEGFR3 hFc is 2-15 ng/mL. 2. Scatchard analysis showed the affinity constant (Kd) of recombinant human VEGF-C bound to recombinant human VEGFR3 was 1.4 nM. 3. Measured in a cell proliferation assay using human umbilical vein endothelial cells (HUVEC). The ED50 for this effect is typically 0.1-0.5 μg/mL. Endotoxin < 1.0 EU per 1μg of the protein by the LAL method. Concentration 0.95 mg/mL Formulation Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. Normally 5% - 8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in  appropriate buffered solutions.   Shipping and Storage The products are shipped with ice pack and can be stored at -20 ℃ for 1 year. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 °C under sterile conditions after reconstitution. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles. Cautions 1. Avoid repeated freeze-thaw cycles. 2. For your safety and health, please wear lab coats and disposable gloves for operation. 3. For research use only.

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Vascular endothelial growth factor D (VEGF-D), also known as c-fos-induced growth factor (FIGF), is a secreted glycoprotein of the VEGF/PDGF family. VEGFs regulate angiogenesis and lymphangiogenesis during development and tumor growth, and are  characterized by eight conserved cysteine residues that form a cystine knot structure. VEGF-C and VEGF-D, which share 23% amino acid (aa) sequence identity, are uniquely expressed as preproproteins that contain long N- and C-terminal propeptide extensions around the VEGF homology domain (VHD). Proteolytic processing of the 354 aa VEGF-D preproprotein creates a secreted proprotein. Further processing by extracellular serine proteases, such as plasmin or furin-like proprotein convertases, forms mature VEGF-D consisting of non- covalently linked 42 kDa homodimers of the 117 aa VHD. Mature human VEGF-D shares 94%, 95%, 99%, 97% and 93% aa identity with mouse, rat, equine, canine and bovine VEGF-D, respectively. It is expressed in adult lung, heart,  muscle, and small intestine, and is most abundantly expressed in fetal lungs and skin. Mouse and human VEGF-D are ligands for VEGF Receptor 3 (VEGF R3, also called Flt-4) that are active across species and show enhanced affinity when processed. Processed human VEGF-D is also a ligand for VEGF R2, also called Flk-1 or KDR. VEGF R3 is strongly expressed in lymphatic endothelial cells and is essential for regulation of the growth and differentiation of lymphatic endothelium. While VEGF-C is the critical ligand for VEGF R3 during embryonic lymphatic development, VEGF-D is most active in neonatal lymphatic maturation and bone growth. Both promote tumor lymphangiogenesis .  Product Properties Synonyms c-fos induced growth factor (vascular endothelial growth factor D); FIGF; vascular endothelial growth factor D; VEGFD; VEGF-D; VEGF-DVEGFDc-Fos-induced growth factor Accession O43915 Source HEK293 Cells-derived human VEGFD, Phe 93-Ser 201 with His tag at the C-terminu Molecular Weight The recombinant mature form of human VEGFD consists of 120 amino acids and has a predicted molecular mass of 13.6 kDa. In SDS-PAGE under reducing conditions, it migrates with an apparent molecular mass of 20-22 kDa due to glycosylation. Tag His Purity > 95% by SDS-PAGE. Biological Activity Measured in a cell proliferation assay using human umbilical vein endothelial cells (HUVEC). The ED50 for this effect is 0.3- 1.6 μg/mL Endotoxin < 1.0 EU per 1μg of the protein by the LAL method. Concentration 0.95 mg/mL Formulation Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions.   Shipping and Storage The products are shipped with ice pack and can be stored at -20 ℃ for 1 year. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 °C under sterile conditions after reconstitution. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles. Cautions 1. Avoid repeated freeze-thaw cycles. 2. For your safety and health, please wear lab coats and disposable gloves for operation. 3. For research use only

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Transforming Growth Factor-alpha (TGF-α) , also known as sarcoma growth factor, TGF-type I and ETGF, is a member of the EGF family of cytokines. TGF-α is an EGF-related polypeptide growth factor that signals through the EGF receptor, and stimulates the proliferation of a wide range of epidermal and epithelial cells.Mature TGF-alpha shows approximately 93% amino acid sequence identity with mouse or rat TGF-alpha and is not species specific in its biological effects. Product Properties Synonyms Transforming Growth Factor alpha; TGF-a; TGF-type I; ETGF Accession P01135  Gene ID 7039 Source E.coli-derived Human TGF-a protein, Vla40-Ala89 Molecular Weight Approximately 5.8 kDa, on SDS-PAGE under reducing conditions AA Sequence V VSHFNDCPDS HTQFCFHGTC RFLVQEDKPA CVCHSGYVGA RCEHADLLA Tag None Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder Purity > 95% as determined by SDS-PAG Biological Activity Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. The ED50 for this effect is < 0.4 ng/mL. Endotoxin < 1.0 EU per 1μg of the protein by the LAL method Formulation Lyophilized after extensive dialysis against PBS. Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O or PBS up to 100 μg/ml.For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.   Shipping and Storage The products are shipped with ice pack and can be stored at -80℃ for 1 year from date of receipt. 1week, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 °C under sterile conditions after reconstitution. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles. Cautions 1. For your safety and health, please wear lab coats and disposable gloves for operation. 2. For research use only!

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TGF-beta 2 (transforming growth factor beta 2) is one of three closely related mammalian members of the large TGF-beta superfamily that share a characteristic cysteine knot structure. TGF-beta 1, -2 and -3 are highly pleiotropic cytokines proposed to act as cellular switches that regulate processes such as immune function, proliferation and epithelial-mesenchymal transition. Each TGF-beta isoform has some non-redundant functions; for TGF-beta 2, mice with targeted deletion show defects in development of cardiac, lung, craniofacial, limb, eye, ear and urogenital systems . Human TGF-beta 2 cDNA encodes a 414 amino acid (aa) precursor that contains a 19 aa signal peptide and a 395 aa proprotein. A furin-like convertase processes the proprotein to generate an N-terminal 232 aa latency-associated peptide (LAP) and a C-terminal 112 aa mature TGF- beta 2. Disulfide-linked homodimers of LAP and TGF-beta 2 remain non-covalently associated after secretion, forming the small latent TGF-beta 1 complex. Covalent linkage of LAP to one of three latent TGF-beta binding proteins (LTBPs) creates a large  latent complex that may interact with the extracellular matrix. Mature human TGF-beta 2 shows 100% aa identity with porcine, canine, equine and bovine TGF-beta 2, and 97% aa identity with mouse and rat TGF-beta 2. It demonstrates cross-species activity. Product Properties Synonyms Transforming Growth Factor beta 2 Accession P61812 Gene ID 7042 Source NS0-derived Human TGF-β2 protein, Ala303-Ser414 Molecular Weight Approximately 24 kDa AA Sequence ALDAAYCF RNVQDNCCLR PLYIDFKRDL GWKWIHEPKG YNANFCAGAC PYLWSSDTQH SRVLSLYNTI NPEASASPCC VSQDLEPLTI LYYIGKTPKI EQLSNMIVKS CKCS Tag None Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder Purity > 97% as determined by SDS-PAGE. Biological Activity Measured by its ability to inhibit the IL-4-dependent proliferation of HT‑ 2 mouse T cells. The ED50 for this effect is 0.025-0.25 ng/mL. Endotoxin < 0.1 EU per 1μg of the protein by the LAL method Formulation Lyophilized from 0.2 µm filtered concentrated solution in 35 % Acetonitrile and 0.1 % TFA Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile 4 mM HCl to a concentration of 0.1 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriately buffered solutions.   Shipping and Storage The products are shipped with ice pack and can be stored at -20℃ to -70 °C for 1 year. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles. Cautions 1. Avoid repeated freeze-thaw cycles. 2. For your safety and health, please wear lab coats and disposable gloves for operation. 3. For research use only.

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TGF-beta 2 (transforming growth factor beta 2) is one of three closely related mammalian members of the large TGF-beta superfamily that share a characteristic cysteine knot structure. TGF-beta 1, -2 and -3 are highly pleiotropic cytokines proposed to act as cellular switches that regulate processes such as immune function, proliferation and epithelial-mesenchymal transition. Each TGF-beta isoform has some non-redundant functions; for TGF-beta 2, mice with targeted deletion show defects in development of cardiac, lung, craniofacial, limb, eye, ear and urogenital systems . Human TGF-beta 2 cDNA encodes a 414 amino acid (aa) precursor that contains a 19 aa signal peptide and a 395 aa proprotein. A furin-like convertase processes the proprotein to generate an N-terminal 232 aa latency-associated peptide (LAP) and a C-terminal 112 aa mature TGF- beta 2. Disulfide-linked homodimers of LAP and TGF-beta 2 remain non-covalently associated after secretion, forming the small latent TGF-beta 1 complex. Covalent linkage of LAP to one of three latent TGF-beta binding proteins (LTBPs) creates a large  latent complex that may interact with the extracellular matrix. Mature human TGF-beta 2 shows 100% aa identity with porcine, canine, equine and bovine TGF-beta 2, and 97% aa identity with mouse and rat TGF-beta 2. It demonstrates cross-species activity. Product Properties Synonyms Transforming Growth Factor beta 2 Accession P61812 Gene ID 7042 Source NS0-derived Human TGF-β2 protein, Ala303-Ser414 Molecular Weight Approximately 24 kDa AA Sequence ALDAAYCF RNVQDNCCLR PLYIDFKRDL GWKWIHEPKG YNANFCAGAC PYLWSSDTQH SRVLSLYNTI NPEASASPCC VSQDLEPLTI LYYIGKTPKI EQLSNMIVKS CKCS Tag None Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder Purity > 97% as determined by SDS-PAGE. Biological Activity Measured by its ability to inhibit the IL-4-dependent proliferation of HT‑ 2 mouse T cells. The ED50 for this effect is 0.025-0.25 ng/mL. Endotoxin < 0.1 EU per 1μg of the protein by the LAL method Formulation Lyophilized from 0.2 µm filtered concentrated solution in 35 % Acetonitrile and 0.1 % TFA Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile 4 mM HCl to a concentration of 0.1 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriately buffered solutions.   Shipping and Storage The products are shipped with ice pack and can be stored at -20℃ to -70 °C for 1 year. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles. Cautions 1. Avoid repeated freeze-thaw cycles. 2. For your safety and health, please wear lab coats and disposable gloves for operation. 3. For research use only.

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TGFβ3 is a member of the TGF-β superfamily subgroup, defined by its structural and functional similarities. TGF-β3, along with β1 and β2, acts as a cellular switch to regulate immune function, cell proliferation, and epithelial-mesenchymal transition. The human TGF-β3 cDNA encodes a 412 amino acid (aa) precursor, which contains a 20 aa signal peptide and a 392 aa protein. Mature human TGF-β3 shares 100%, 99%, and 98% homology with mouse/dog/horse, rat, and pig TGF-β3, respectively. TGF-β3 has a high affinity for TGF-βRII, a type II serine/threonine kinase receptor. This receptor phosphorylates and activates the type I serine/threonine kinase receptor TGF-βRI or ALK-1 to regulate transcription through Smad phosphorylation. The product is provided in the form of a lyophilized (freeze-dried) powder, characterized by high activity, high purity, and low endotoxin levels. Product Properties Synonyms TGF-β3; ARVD; ARVD1; FLJ16571; LDS5; RNHF; TGFB3; TGFbeta 3; TGF-beta 3; TGF-beta3; TGF-beta-3; transforming growth factor beta-3; transforming growth factor beta 3 Uniprot No. P10600 Expression System and Range CHO-derived Human TGF-β3, Ala 301-Ser 412 Molecular Weight Approximately 13 kDa AA Sequence MALDTNYCFRN LEENCCVRPL YIDFRQDLGW KWVHEPKGYY ANFCSGPCPY LRSADTTHST VLGLYNTLNP EASASPCCVP QDLEPLTILY YVGRTPKVEQ LSNMVVKSCK CS Appearance White powder, Colorless clear liquid after reconstitution Purity ≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining. Biological Activity Measured in a cell inhibition assay using TF-1 cells at IL4 presence. The ED50 for this effect is ≤0.2ng/mL. Endotoxin < 10 EU/mg of the protein by the LAL method Formulation Lyophilized from a 0.22 μm-filtered solution containing 100 mM Glycine, 150 mM NaCl, 5% mannitol and 0.01% Tween 80, pH 4.0   Method of Use It is recommended to centrifuge before opening the cap to bring the contents to the bottom, and then resuspend with sterile deionized water. Transportation and Storage Methods Store at -20°C, with a one-year shelf life upon receipt. After resuspension, store at 2~8°C for a shelf life of 7-10 days. After resuspension, store at -85 to -65°C for a shelf life of six months. It is recommended to aliquot and freeze for the first use to avoid repeated freeze-thaw cycles. Cautions 1.For your safety and health, please wear a lab coat and use disposable gloves when operating. 2.This product is intended for scientific research purposes only.

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Wnt3a, one of Wnt family members, plays key roles in regulating pleiotropic cellular functions, including self-renewal, proliferation, differentiation, and motility. The Wnt family comprises 19 human proteins, including Wnt1, Wnt2, Wnt2b (Wnt13), Wnt3, Wnt3a, Wnt4, Wnt5a, Wnt5b, Wnt6, Wnt7a, Wnt7b, Wnt8a, Wnt8b, Wnt9a (Wnt14), Wnt9b (Wnt14b), Wnt10a, Wnt10b, Wnt11, and Wnt16. These genes encode secreted glycoproteins that are rich in cysteine. Wnts can combine with cell membrane receptors that play a critical role in autocrine regulation and/or participate in paracrine modification by binding to adjacent cell membrane receptors. The signal transduction pathway mediated by Wnt genes is called the Wnt signaling pathway. Accumulating evidence has suggested that Wnt3a promotes or suppresses tumor progression via the canonical Wnt signaling pathway depending on cancer type. In addition, the roles of Wnt3a signaling can be inhibited by multiple proteins or chemicals. Human Wnt-3a shares 96% aa identity with mouse mouse, bovine and canine Wnt-3a, and 89%, 86% and 84% aa identity with chicken, Xenopus and zebrafish Wnt-3a, respectively. It also shares 87% aa identity with Wnt3. During embryonic development, Wnt-3a is necessary for proper development of the hippocampus, anterior-posterior patterning, somite development, and tailbud formation. Wnt-3a also promotes self-renewal of  hematopoietic stem cells, neural stem cells, and embryonic stem cells. Product Properties Synonyms Wingless-type MMTV Integration Site Family, Member 3 Uniprot No. P56704 GeneID 8978 Source HEK293 cells-derived Human wnt-3a, Ser19-Lys352 Molecular Weight Approximately 63.3 kDa AA Sequence SY PIWWSLAVGP QYSSLGSQPI LCASIPGLVP KQLRFCRNYV EIMPSVAEGI KIGIQECQHQ  FRGRRWNCTT VHDSLAIFGP VLDKATRESA FVHAIASAGV AFAVTRSCAE GTAAICGCSS RHQGSPGKGW KWGGCSEDIE FGGMVSREFA DARENRPDAR SAMNRHNNEA GRQAIASHMH LKCKCHGLSG SCEVKTCWWS QPDFRAIGDF LKDKYDSASE MVVEKHRESR GWVETLRPRY TYFKVPTERD LVYYEASPNF CEPNPETGSF GTRDRTCNVS SHGIDGCDLL CCGRGHNARA ERRREKCRCV FHWCCYVSCQ ECTRVYDVHT CK Tag Fc Purity > 75% as determined by SDS-PAGE. Activity Measured by its binding ability in a ELISA method. Immobilized Human Wnt-3a at 10 μg/ml (100 μl/well) can bind bind Wnt-3a mouse monoclonal antibody. Endotoxin < 1.0 EU per 1μg of the protein by the LAL method. Formulation Lyophilized from a 0.2 µm filtered concentrated solution in PBS. Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions. Transportation and Storage Methods Transport with ice packs. Store at -20°C to -80°C with a one-year shelf life. It is recommended to aliquot and freeze for the first use to avoid repeated freeze-thaw cycles. Cautions 1.Avoid repeated freezing and thawing. 2.For your safety and health, please wear a lab coat and use disposable gloves during operations. 3.This product is for research use only!

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HGF is a potent, mesenchymally-derived mitogen for mature parenchymal hepatocytes, and acts as a growth factor for a broad spectrum of tissues and cell types. HGF signals through a transmembrane tyrosine kinase receptor known as MET. Activities of HGF include the induction of cell proliferation, motility, morphogenesis, inhibition of cell growth, and enhancement of neuron survival. HGF is a crucial mitogen for liver regeneration processes, especially after partial hepatectomy and other liver injuries. HGF, also known as scatter factor and hepatopoietin A, is a pleiotropic protein in the plasminogen subfamily of S1 peptidases. It is a multidomain molecule that includes an N-terminal PAN/APPLE‑like domain, four Kringle domains, and a serine proteinase-like domain that has no detectable protease activity. Product Properties Synonyms Scatter Factor (SF), Hepatopoietin (HPTA) Accession P14210 Gene ID 3082 Source CHO-derived Human HGF protein, Gln32-Ser728 Molecular Weight 88~90 kDa (single chain), 59~61 kDa (alpha chain), 30~34 kDa (beta chain), on SDS-PAGE under reducing conditions. Tag None Purity > 95% by SDS-PAGE Biological Activity ED50 < 10.0 ng/ml, measured in a cell proliferation assay using 4MBr5 cells, corresponding to a specific activity of > 1.0 × 10 5 units/mg. Endotoxin < 0.2 EU per 1μg of the protein by the LAL method. Formulation Lyophilized after extensive dialysis against PBS Reconstitution It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O or PBS up to 100 μg/ml. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Shipping and Storage The products are shipped with ice pack and can be stored at -80℃ for 1 year. 1 week, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 °C under sterile conditions after reconstitution. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles. Cautions 1. Avoid repeated freeze-thaw cycles. 2. For your safety and health, please wear lab coats and disposable gloves for operation. 3. For research use only.

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TRANCE, also known as receptor activator of NF-kappa B ligand (RANKL), TNF-related activation-induced cytokine (TRAC), osteoprotegerin ligand (OPGL), and osteoclast differentiation factor (ODF), is a member of the tumor necrosis factor (TNF) family. Mouse TRANCE cDNA encodes a type II transmembrane protein consisting of 316 amino acids, with a predicted cytoplasmic domain of 48 amino acids and an extracellular domain of 247 amino acids. The extracellular domain contains two potential N-linked glycosylation sites. Mouse and human TRANCE proteins share 85% homology. TRANCE is primarily expressed in T cells and T cell-rich organs, such as the thymus and lymph nodes. This protein has functions including the induction of c-jun N-terminal kinase (JNK) activation, enhancement of T cell growth and dendritic cell function, induction of osteoclastogenesis, and lymph node organogenesis. Product Properties Synonyms soluble Receptor Activator of NF-κB Ligand, TNFSF11, TRANCE (TNF-related activation-induced cytokine), OPGL, ODF Uniprot No. O35235 Expression Range and Expression System E.coil-derived mouse RANKL Molecular Weight Approximately 19.4 kDa. A A Sequence MPAMMEGSWLDVAQRGKPEAQPFAHLTINAASIPSGSHKVTLSSWYHDRGWAKISNMTLSNG KLRVNQDGFYYLYANICFRHHETSGSVPTDYLQLMVYVVKTSIKIPSSHNLMKGGSTKNWSGN SEHFYSINVGGFFKLRAGEEISIQVSNPSLLDPDQDATYFGAFKVQDID Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 98% as determined by SDS-PAGE. Biological Activity Measure by its ability to induce osteoclast differentiation in RAW264.7 cells. The ED50 for this effect is 2 ng/mL. Endotoxin < 0.1 EU per 1μg ofthe protein by the LAL method. Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 1×PBS, pH 8.0. Instructions for Use 1.It is recommended to reconstitute the lyophilized powder with sterile water, ensuring the solution concentration is not less than 100 μg/mL, and let it stand for at least 20 minutes to fully dissolve. 2.After reconstitution, the solution can be further diluted and aliquoted, stored at 2-8°C with a shelf life of 1 month, and at -20°C with a shelf life of 3 months; avoid repeated freeze-thaw cycles. 3.When further diluting and aliquoting the reconstituted solution, a certain amount of carrier protein should be added (0.1% BSA, 10% FBS, or 5% HSA). For serum-free experimental requirements, it can be replaced with a 5% trehalose solution as the carrier. Shipping and Storage Transport with ice packs. Store at -20°C with a one-year shelf life.It is recommended to aliquot and freeze for the first use to avoid repeated freeze-thaw cycles. Cautions 1.For your safety and health, please wear a lab coat and use disposable gloves when handling. 2.This product is for research use only!

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GCP-2 also known as CXCL6, is a CXC chemokine initially isolated as a neutrophil chemoattractant from the MG-63 osteosarcoma cell line. Among human CXC chemokines, GCP-2 is most closely related to ENA-78 (78% amino acid (aa) sequence identity in the mature peptide region and 86% identity in the signal sequence). The structure and sequence of the genes for human GCP-2 and ENA-78 also exhibit close similarity suggesting the two genes may have originated from a gene duplication. LIX (LPS-induced CXC chemokine) was initially cloned as a gene induced by LPS in mouse fibroblasts. The predicted LIX protein sequence is identical to a previously purified mouse protein designated mouse GCP-2 based on its amino sequence similarity (60% sequence identity) to human GCP-2. Mouse GCP-2/LIX is also 54% identical with human ENA-78 at the amino acid sequence level. Product Properties Synonyms Chemokine alpha 3, CXCL6, GCP-2, CXCL6, member b Accession P80221 GeneID 281735 Source E.coli-derived bovine GCP-2/CXCL6 protein, Gly37-Asn112 Molecular Weight Approximately 8.0 kDa. AA Sequence GPVAAVVREL RCVCLTTTPG IHPKTVSDLQ VIAAGPQCSK VEVIATLKNG REVCLDPEAP LIKKIVQKIL DSGKNN Tag None Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity >97% by SDS-PAGE and HPLC analyses Biological Activity The biological activity determined by a chemotaxis bioassay using human neutrophils is in a concentration range of 10-50 ng/mL. Fully biologically active when compared to standard. Endotoxin < 0.1 EU per 1μg of the protein by the LAL method. Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, 500 mM NaCl, pH 7.0 Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions. Shipping and Storage The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles. Cautions 1. Avoid repeated freeze-thaw cycles. 2. For your safety and health, please wear lab coats and disposable gloves for operation. 3. For research use only!

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Chemokine CXCL9 is a member of the CXC family and has an important role in the chemotaxis of immune cells. The mouse CXCL9 shares 75% and 88% a.a. sequence identity with human and rat CXCL9. Accumulated experimental evidence supports that monokine induced by interferon (IFN)-gamma (CXCL9), a member of CXC chemokine family and known to attract CXCR3- (A and B) T lymphocytes, is involved in the pathogenesis of physiologic diseases during their initiation and their maintenance. It is a cytokine that affects the growth, movement, or activation state of cells that participate in immune and inflammatory response and chemotactic for activated T-cells. Product Properties Synonyms C-X-C motif chemokine 9, CXCL9, Gamma-interferon-induced Monokine, Humig, MIG, Small-inducible Cytokine B9 Accession A9QWP9 GeneID 513990 Source E.coli-derived bovine MIG/CXCL9 protein, Val22-Thr125 . Molecular Weight Approximately 11.9 kDa. AA Sequence VPAIRNGRCS CINTSQGMIH PKSLKDLKQF APSPSCEKTE IIATMKNGNE ACLNPDLPEV KELIKEWEKQ VNQKKKQRKG KKYKKTKKVP KVKRSQRPSQ KKTT Tag None Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity >96% by SDS-PAGE and HPLC analyses. Biological Activity The biological activity determined by a chemotaxis bioassay using human lymphocytes is in a concentration range of 0.1-1.0 ng/mL. Fully biologically active when compared to standard. Endotoxin < 0.1 EU per 1μg of the protein by the LAL method. Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.0, 500 mM NaCl. Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions. Shipping and Storage The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles. Cautions 1. Avoid repeated freeze-thaw cycles. 2. For your safety and health, please wear lab coats and disposable gloves for operation. 3. For research use only!

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CXCL4, also called PF4, is a small cytokine belonging to the CXC chemokine family and it is also known as chemokine (C-X-C motif) ligand. Mature mouse CXCL4 shares 76%, 88%, 64%, 64% and 63% amino acid sequence identity with human, rat, ovine, porcine and bovine CXCL4, respectively. Recombinant mouse CXCL4 contains 76 amino acids which is a single non-glycosylated polypeptide chain. CXCL4 can be antiproliferative and antiangiogenic, at least in part via interfering with FGF-2 and VEGF heparin binding and thus inhibiting their signaling. Tumor tissue revealed up-regulation of CXCL14 in cancer-associated fibroblasts of a majority of prostate cancer. Fibroblasts overexpressing CXCL14 promoted the growth of prostate cancer xenografts, and increased tumor angiogenesis and macrophage infiltration. Product Properties Synonyms chemokine (C-X-C motif) ligand 4, C-X-C motif chemokine 4, CXCL4, CXCL4iroplact, Iroplact, MGC138298, Oncostatin-A, PF4, platelet factor 4 Accession P02777 Unigene Bt.11581. Source E.coli-derived bovine PF-4/CXCL4 protein, Glu1-Ser88. Molecular Weight Approximately 9.5 kDa. AA Sequence ESSFPATFVP LPADSEGGED EDLQCVCLKT TSGINPRHIS SLEVIGAGTH CPSPQLLATK KTGRKICLDQ QRPLYKKILK KLLDGDES Tag None Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity >95% by SDS-PAGE and HPLC analyses. Biological Activity The biological activity determined by a chemotaxis bioassay using human neutrophils is in a concentration of 10-100ng/ml. Fully biologically active when compared to standard. Endotoxin < 0.1 EU per 1μg of the protein by the LAL method. Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, 500 mM NaCl, pH 7.0. Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions. Shipping and Storage The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles. Cautions 1. Avoid repeated freeze-thaw cycles. 2. For your safety and health, please wear lab coats and disposable gloves for operation. 3. For research use only!

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