Description
Description
Bone morphogenetic proteins (BMPs) constitute a subfamily of structurally related signaling proteins within the TGF-β superfamily. Members of this superfamily are widely distributed throughout the body and are involved in various physiological processes both prenatally and postnatally. Like BMP-7, BMP-4 is also involved in the development and maintenance of bone and cartilage. Reduced expression of BMP4 is associated with many skeletal diseases, including the hereditary condition progressive diaphyseal dysplasia. Mature mouse and human BMP-4 have 98% amino acid (aa) homology.
This product features high activity, high purity, and low endotoxin levels. It has been validated and tested for bioactivity, endotoxin levels, and SDS-PAGE to ensure the consistency of biological activity and batch-to-batch uniformity. Our product is provided in a carrier-free form, making it highly suitable for research and production of cell culture, ELISA, or immunoblotting standards.
Product Properties
|
Synonyms
|
Bone Morphogenetic Protein 4;BMP4;BMP2B; BMP-2B; BMP2B1;
|
|
Uniprot No.
|
P21275
|
|
Source
|
E.coil-derived Mouse BMP-4 protein,Lys303-Arg408 with His tag at the C-terminus
|
|
Amino Acid
|
MKKNKNCRRHSLYVDFSDVGWND WIVAPPGYQAFYCHGDCPFPLADHL NSTNHAIVQTLVNSVNSSIPKACCVP TELSAISMLYLDEYDKVVLKNYQEMV VEGCGCR
|
|
Molecular Weight
|
Approximately 14 kDa.
|
|
Biological Activity
|
Measure by its ability to induce alkaline phosphatase production by ATDC5 cells. The ED50 for this effect is 10 ng/mL. The specific activity of recombinant mouse BMP-4 is > 1 x 100000 IU/mg.
|
|
Purity
|
> 98% as determined by SDS-PAGE.
|
|
Endotoxin
|
<0.1 EU per 1μg of the protein by the LAL method.
|
|
Formulation
|
Lyophilized from a 0.2 μm filtered concentrated solution in 1×PBS, pH4.5.
|
|
Reconstitution
|
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.
|
Shipping and Storage
Ice pack shipping. Store at -20°C with a one-year shelf life.
Recommended to aliquot and freeze upon first use to avoid repeated freezing and thawing.
Cautions
1.Please operate with lab coats and disposable gloves,for your safety.
2.This product is for research use only.
Usage
1. It is recommended to reconstitute the lyophilized powder with sterile water, ensuring that the solution concentration is not less than 100 μg/mL, and let it stand for at least 20 minutes to fully dissolve.
2. After reconstitution, the solution can be further diluted and aliquoted, stored at 2-8°C with a shelf life of 1 month, and at -20°C with a shelf life of 3 months; avoid repeated freezing and thawing.
3. When further diluting and aliquoting the reconstituted solution, a certain amount of carrier protein should be added (0.1% BSA, 10% FBS, or 5% HSA). For experiments that require serum-free conditions, it can be replaced with a 5% trehalose solution as the carrier.
