NGS Library Prep

Concanavalin A (ConA) Magnetic Beads are superparamagnetic polymer microspheres with Concanavalin A covalently coupled to their surface. These beads are characterized by uniform dispersion and strong magnetic responsiveness. In the presence of Ca²⁺ and Mg²⁺ ions, ConA magnetic beads enable rapid and efficient isolation and purification of biomolecules such as polysaccharides, glycoproteins, and glycolipids through the specific affinity of ConA for terminal α-D-mannosyl and α-D-glucosyl groups. Additionally, ConA magnetic beads offer a convenient method for cell isolation or immobilization, minimizing cell loss during subsequent washing steps. They can also be used for nuclear isolation and immobilization, and are directly applicable in CUT & RUN and CUT & TAG (innovative techniques derived from ChIP-seq) assays. Specifications Cat.No. N210601E / N210601S Size 200 μL / 1 mL Properties Matrix Magnetic polymer microspheres Ligand Concanavalin A (Con A) Binding Capacity 105 Cell/nuclei isolation, glycoprotein purification, Cut-Run, Cut-Tag Particle Size 1 μm Bead Concentration 10 mg/mL Applications Cell/nuclei isolation, glycoprotein purification, Cut-Run, Cut-Tag Storage Buffer PBS (pH7.4), 0.01% Tween-20, 0.05% Proclin-300 Storage This product should be stored at 2~8℃ for 2 years. Documents： Manual

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Nanobody-Tn5 is a fusion protein combining nanobodies with Tn5 transposase, designed to generate anti-mouse Tn5 and anti-rabbit Tn5 that specifically bind to primary antibodies from two different species (mouse and rabbit). When these Nanobody-Tn5 complexes are pre-loaded with adapter sequences containing distinct indexes, the Tn5 enzyme cleaves chromatin and simultaneously inserts the indexed adapters into the fragmented DNA. This allows differentiation between genomic regions targeted by mouse primary antibodies and those targeted by rabbit primary antibodies based on the unique index sequences. This product is a "naked" enzyme and requires user assembly with adapter sequences. Specifications Cat.No. N210605S / N210605M Size 10 μL / 100 μL Components Components No. Name N210605S N210605M N210605-A Anti-mouse Tn5 10 μL 100 μL N210605-B Anti-rabbit Tn5 10 μL 100 μL N210605-C Assemble Buffer 10 μL 100 μL Storage This product should be stored at -85℃~-65℃ for 1 year. Documents： Manual

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Anti-rabbit Nanobody-Tn5 is a fusion protein combining a nanobody with Tn5 transposase, designed for use in Multi-CUT&Tag assays. This product is suitable for CUT&Tag experiments utilizing rabbit primary antibodies. This is a "naked" enzyme and requires user assembly with adapter sequences. Specifications Cat.No. N210604S / N210604M Size 12 T / 48 T Components Name N210604S N210604M Anti-rabbit Tn5 12 μL 48 μL Storage This product should be stored at -25~-15℃ for 1 year. Documents： Manual

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NGS dsDNA Methyl Library Prep Kit for IlluminaTM is a double-stranded DNA methylation library preparation kit developed specifically for the IlluminaTM high-throughput sequencing platform. This kit enables the construction of methylation libraries compatible with IlluminaTM sequencing through ligation of specific methylated adapters, followed by bisulfite or enzymatic conversion, and a single amplification step. All components of the kit have undergone rigorous quality control and functional validation to ensure consistent library output. It supports various types of DNA samples, including gDNA, cfDNA, and FFPE-derived DNA. The recommended input DNA amount ranges from 10 ng to 1000 ng, and the kit is compatible with mainstream conversion methods available on the market. Specifications Cat.No. N210602E / N210602S / N210602M Size 8 T / 24 T / 96 T Components Components No. Name N210602E N210602S N210602M N210602-A Endprep Buffer 56 μL 168 μL 672 μL N210602-B Endprep Enzyme 24 μL 72 μL 288 μL N210602-C Ligation Enhancer 240 μL 720 μL 3×960 μL N210602-D Rapid T4 DNA Ligase 80 μL 240 μL 960 μL N210602-E Methyl Stubby Adapter 40 μL 120 μL 480 μL N210602-F 2 × CanaceTM Uracil + PCR Mix 2.0 200 μL 600 μL 2×1200 μL Storage This product should be stored at -25~-15℃ for 1 year. Documents： Manual

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phi29 DNA polymerase is derived from the Bacillus subtilis bacteriophage. After genetic engineering, it exhibits excellent strand displacement and processive synthesis capabilities, enabling the synthesis of DNA fragments up to 70 kb in length. Additionally, it possesses strong 3’→5’ exonuclease proofreading activity. Primer 3’ end modifications are recommended in the recommended reaction system to prevent degradation. This enzyme is commonly used in in vitro plasmid synthesis and whole genome amplification. Specifications Cat.No. N210508S / N210508M / N210508L Size 250 U / 1000 U / 5000 U Unit Definition The amount of enzyme required to incorporate 0.5 pmol of dNTP into acid-insoluble material in 10 minutes at 30°C. Heat Inactivation 65°C for 10 min. Components Components No. Name N210508S N210508M N210508L N210508-A phi29 DNA Polymerase (10 U/μL） 25 μL 100 μL 500 μL N210508-B 10×phi29 Reaction Buffer 100 μL 400 μL 2×1 mL Storage This product should be stored at -25~-15℃ for 2 years. Documents： Manual

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DNA Polymerase I synthesizes DNA in the 5′→3′ direction complementary to a template in the presence of a primer (DNA or RNA) and dNTPs as substrates. This enzyme has a molecular weight of approximately 109,000 and possesses both double-stranded specific 5′→3′ exonuclease activity and single-stranded specific 3′→5′ exonuclease activity. It can be used alongside DNase I for nick translation and with RNase H for second-strand cDNA synthesis. Specifications Cat.No. N210511S / N210511M / N210511L Size 500 U / 2500 U / 10000 U Components Components No. Name N210511S N210511M N210511L N210511-A DNA polymerase I (10 U/μL) 50 μL 250 μL 1 mL N210511-B 10 × DNA Polymerase I Reaction Buffer 1 mL 1 × 5 mL 4 × 5 mL Storage This product should be stored at -25~-15℃ for 2 years. Documents： Manual

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T4 Polynucleotide Kinase is a polynucleotide 5'-hydroxyl kinase capable of catalyzing the transfer of the γ-phosphate group from ATP to the 5'-hydroxyl termini of oligonucleotide chains (double-stranded or single-stranded DNA or RNA) and to 3'-monophosphate nucleotides. This reaction is reversible. It also has 3'-phosphatase activity, removing 3'-phosphate groups from oligonucleotides' 3'-phosphorylated ends, deoxy-3'-monophosphate nucleotides, and deoxy-3'-diphosphate nucleotides. In the presence of ADP, T4 PNK exhibits 5'-phosphatase activity, facilitating the exchange of terminal 5'-phosphate groups between 5'-phosphorylated oligo/polynucleotides and ATP. Suitable for constructing DNA libraries and end-labeling to make probes. Specifications Cat.No. N210510S / N210510M / N210510L Size 500 U / 2500 U / 10000 U Concentration 10 U/µL Source Recombinantly expressed in E. coli Enzyme Activity Definition The amount of enzyme required to catalyze the transfer of 1 nmol [γ-³²P] ATP in 30 minutes at 37°C. Components Components No. Name N210510S N210510M N210510L N210510-A T4 polynucleotide Kinase (10 U/μL) 50 μL 250 μL 1 mL N210510-B 10×T4 PNK Buffer 500 μL 1 mL 2×1 mL [Note]: The 10×T4 PNK Buffer does not contain ATP; Users need to add it themselves with a recommended final concentration of 1 mM or use T4 DNA ligase buffer. Storage This product should be stored at -25~-15℃ for 1 year. Documents： Manual

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T4 DNA Polymerase catalyzes the synthesis of DNA in a 5'→3' direction in the presence of a template and primer. It possesses 3'→5' exonuclease activity but lacks 5'→3' exonuclease activity. Specifications Cat.No. N210507E / N210507S / N210507M / N210507L Size 100 U / 500 U / 2000 U / 5000 U Components Components No. Name N210507E N210507S N210507M N210507L N210507-A T4 DNA polymerase (5 U/μL) 20 μL 100 μL 400 μL 1 mL N210507-B 10 × Blue Buffer 100 μL 250 μL 500 μL 1.25 mL Unit Definition One unit (U) of enzyme is defined as the amount required to catalyze the incorporation of 10 nmol of deoxyribonucleotides (dNTPs) into polynucleotides in 30 minutes at 37°C. Product Applications Blunting of DNA 5' or 3' overhangs; Synthesis of labeled DNA probes through displacement reactions; Second strand synthesis during site-directed mutagenesis; PCR product cloning without the need for ligation reactions. Storage Shipped with dry ice.This product should be stored at -25℃ ~-15℃ for 1 year. Notes 1. For your safety and health, wear lab coats and disposable gloves during operation. 2. This product is for research use only! Documents： Manual

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This kit utilizes a unique magnetic bead and buffer system that specifically binds RNA, effectively removing proteins, salt ions, and other contaminants. It is commonly used for the purification of total RNA samples after rRNA depletion, in vitro transcribed RNA, labeled RNA products, and synthetic RNA. The purified RNA is suitable for downstream applications such as RNA library construction, RT-PCR, qRT-PCR, microarray analysis, Northern blot, and RNA interference (RNAi). Specifications Cat.No. N210363E / N210363S / N210363M / N210363L Size 1 mL / 5 mL / 60 mL Components Name N210363E N210363S N210363M N210363L NGS RNA Purification Beads 1 mL 5 mL 60 mL 450 mL Storage Store at 2–8 °C. Valid for 2 years. Documents： Manual

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NGS DNA Selection Beads are based on the SPRI (Solid Phase Reverse Immobilization) principle and, in combination with a carefully optimized buffer system, can be used for DNA fragment size selection and purification during library construction in next-generation sequencing. This product is compatible with DNA and RNA library preparation kits from various brands and has the same usage method as the widely used AMPure XP Beads. Both the fragment recovery efficiency and the library size distribution are highly consistent with those of AMPure XP Beads. Specifications Cat.No. N210362E / N210362S / N210362M / N210362L Size 1 mL / 5 mL / 60 mL / 450 mL Components Name N210362E N210362S N210362M N210362L NGS DNA Selection Beads 1 mL 5 mL 60 mL 450 mL Storage Store at 2~8 °C. Valid for 18 months. Documents： Manual

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The NGS mRNA Isolation Master Kit is an upgraded magnetic bead-based reagent kit specifically designed for the purification of mRNA. The mRNA Capture Beads in the kit are micrometer-sized superparamagnetic microspheres conjugated with Oligo(dT), which specifically bind to the poly(A) tails of mRNA molecules. This enables the efficient isolation and purification of mRNA from 10 ng to 4 μg of high-quality total RNA. Specifications Cat.No. N210361S / N210361M / N210361L Size 8T / 24 T / 96 T Components Components No. Name N210361S N210361M N210361L N210361-A mRNA Capture Beads 2.0 400 μL 1.2 mL 4.8 mL N210361-B Beads Binding Buffer 2.0 400 μL 1.2 mL 4.8 mL N210361-C Beads Wash Buffer 2.0 5 mL 15 mL 60 mL N210361-D Tris Buffer 2.0 400 μL 1.2 mL 4.8 mL N210361-E Nuclease-free water 1 mL 1 mL 4 mL Storage Store at 2–8 °C.Valid for 1 year. Documents： Manual

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Specifications Cat.No. N210305S / N210305M Size 500 tubes/box / 10 boxes/carton Material 0.5 mL thin-wall PCR tubes made from high-quality imported polypropylene. Storage Shipped at room temperature. Store at room temperature, valid for 3 years. Features 1. Designed for use with QubitTM fluorometers. 2. Thin and uniformly thick walls ensure optimal thermal conductivity. 3. Excellent sealing performance between cap and tube body prevents evaporation and contamination; easy to open. 4. Pyrogen-free, and free of RNase and DNase. Notes 1. This product is for research use only. 2. Please operate with lab coats and disposable gloves，for your safety. Documents： Manual  

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The ssDNA Assay Kit is a simple, sensitive, and accurate fluorescent quantification kit for single-stranded DNA (ssDNA), exhibiting excellent linearity in the range of 1–200 ng. The kit includes a fluorescent detection reagent, buffer, and ssDNA standards. Before use, dilute the fluorescent reagent with the provided buffer to prepare the working solution. Then add the ssDNA sample, and measure fluorescence using a fluorescence microplate reader or a QubitTM fluorometer. Although the kit does not show higher specificity for ssDNA over dsDNA, it demonstrates good tolerance to common contaminants such as proteins, salts, and detergents. Specifications Cat.No. N210304S / N210304M Size 100 T / 500 T Components Components No. Name Concentration N210304S N210304M N210304-A ssDNA Reagent 200× in DMSO 250 μL 1.25 mL N210304-B ssDNA Buffer Not applicable 50 mL 250 mL N210304-C ssDNA Standard 1 0 ng/μL in TE buffer 1 mL 5×1 mL N210304-D ssDNA Standard 2 20 ng/μL in TE buffer 1 mL 5×1 mL Storage Shipped on ice packs. Store at 2–8 °C in the dark.Valid for 1 year.Avoid repeated freeze-thaw cycles. Documents： Manual

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The dsDNA BR Assay Kit is a simple, sensitive, accurate, and wide-range fluorescent quantification kit for double-stranded DNA (dsDNA). This kit exhibits excellent linearity in the range of 2–1000 ng, making it suitable for a broad range of applications. The kit includes fluorescent detection reagent, buffer, and dsDNA BR standards. Prior to use, dilute the fluorescent detection reagent with the provided buffer to prepare the working solution. Then add the dsDNA sample, generate a standard curve, and measure fluorescence using a fluorescence microplate reader or a QubitTM fluorometer. The kit shows good tolerance to common contaminants such as proteins and salts. Specifications Cat.No. N210303S / N210303M Size 100 T /500 T Components Components No. Name Concentration N210303S N210303M N210303-A dsDNA BR Reagent 200×concentrate in DMSO 250 μL 1.25 mL N210303-B dsDNA BR Buffer Not applicable 50 mL 250 mL N210303-C dsDNA BR Standard 1 0 ng/μL in TE buffer 1 mL 5×1 mL N210303-D dsDNA BR Standard 2 100 ng/μL in TE buffer 1 mL 5×1 mL Storage Store at 2–8 °C in the dark for up to 6 months. Shipped on ice packs.Avoid repeated freeze-thaw cycles. Documents： Manual

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The 1×dsDNA HS Assay Kit is a fast, sensitive, and accurate fluorescent quantification kit for double-stranded DNA (dsDNA). This kit offers high specificity for dsDNA and exhibits excellent linearity in the range of 0.2–100 ng. The assay is simple and convenient—just mix the sample with the working solution and read the fluorescence signal using a Qubit fluorometer or a fluorescence microplate reader. With its ease of use and reliable results, this kit is an ideal choice for high-throughput DNA quantification in next-generation sequencing (NGS) applications, such as input DNA quantification and DNA library quantification. The kit is also highly tolerant to common contaminants such as proteins and salts. Specifications Cat.No. N210302S / N210302M Size 100 T/500 T Components Components No. Name Concentration N210302S N210302M N210302-A 1×dsDNA HS Working Solution 1×concentrate in DMSO 50 mL 250 mL N210302-B dsDNA Standard 1 0 ng/μL in TE buffer 1 mL 5×1 mL N210302-C dsDNA Standard 2 10 ng/μL in TE buffer 1 mL 5×1 mL Storage Shipped on ice packs. Store at 2–8 °C in the dark, valid for 1 year. Documents： Manual

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The dsDNA HS Assay Kit for QubitTM is a fast, sensitive, and accurate fluorescent quantification kit for double-stranded DNA (dsDNA). This kit offers high specificity for dsDNA and exhibits excellent linearity in the range of 0.2–100 ng. The assay is simple and convenient—just mix the sample with the working solution and read the fluorescence signal using a QubitTM fluorometer or a fluorescence microplate reader. With its ease of use and reliable results, this kit is an ideal choice for high-throughput DNA quantification in next-generation sequencing (NGS) applications, such as input DNA quantification and DNA library quantification. The kit is also highly tolerant to common contaminants such as proteins and salts. Specifications Cat.No. N210301S / N210301M Size 100 T / 500 T Components Components No. Name Concentration N210301S N210301M N210301-A dsDNA Reagent 200×concentrate in DMSO 250 μL 1.25 mL N210301-B dsDNA Buffer Not applicable 50 mL 250 mL N210301-C dsDNA Standard 1 0 ng/μL in TE buffer 1 mL 5×1 mL N210301-D dsDNA Standard 2 10 ng/μL in TE buffer 1 mL 5×1 mL Storage Shipped on ice packs. Store at 2–8 °C in the dark, valid for 1 year. Documents： Manual

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NGS PreMix RNA Library Prep Kit(dNTP) is a total RNA sequencing library preparation kit compatible with both IlluminaTM and MGITM platforms. This product is available in standard tube format and pre-loaded plate format. The pre-loaded plate format eliminates the need for manual reagent preparation or aliquoting, and can be directly adapted for automated library preparation, offering greater convenience. The kit includes all necessary reagents for RNA fragmentation, reverse transcription, strand-specific ds-cDNA synthesis, and library amplification. It is compatible with upstream workflows involving mRNA enrichment or rRNA depletion. Engineered for enhanced user safety and data quality, the kit features an optimized reverse transcription system that achieves high strand specificity without the use of actinomycin D. Every component undergoes stringent quality control and functional testing to ensure robust library prep performance, batch-to-batch consistency, and high reproducibility. Specifications Cat.No. N210105S / N210105M / N210105MP Size 24 T / 96 T / 96 T (plate) Components Components No. Name N210105S N210105M N210105MP** N210105-A Frag/Primer Buffer 450 μL 2×900 μL 8×266 μL N210105-B 1st Reaction Module 2.0 192 μL 768 μL 8×120 μL N210105-C 2nd Reaction Module(dNTP) 840 μL 3×1120 μL 8×480 μL N210105-D Ligation Reaction Module 840 μL 3×1120 μL 8×480 μL N210105-E 2×Super CanaceTM II High-Fidelity Mix 600 μL 2×1200 μL 8×340 μL * Primer Mix* / / / [Note]: * This component is not included in the kit. It is not required when using short or incomplete adapters. However, if full-length or complete adapters are used during library preparation, this component must be added separately. This kit is compatible with both IlluminaTM and MGITM platforms. Platform-specific Primer mixes must be purchased separately: Cat# N210782 — RNA Library Prep Primer Mix for MGITM (compatible with complete adapters) Cat# N210701 — Primer Mix for IlluminaTM ** For the layout of reagents in the pre-loaded plate format, please refer to the figure below. The left image shows the reagent layout of the pre-loaded plate format library preparation kit, with each component occupying one column, and each well containing sufficient reagent for 12 library preparation reactions. When using the pre-loaded plate kit with automated instruments, the empty plate positions (columns 6-12) can be filled with beads, adapters, or other reagents according to experimental needs prior to the experiment. It is recommended that each well of the pre-loaded plate format reagent be used up in a single use.  Figure 1. Layout of Reagent Components for Plate-based Library Preparation Kit Storage This product should be stored at -25~-15℃ for 1 year. Documents： Manual

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NGS PreMix RNA Library Prep Kit(Strand-specific) is a strand-specific RNA sequencing library preparation kit compatible with both IlluminaTM and MGITM platforms. This product is available in standard tube format and pre-loaded plate format. The pre-loaded plate format eliminates the need for manual reagent preparation or aliquoting, and can be directly adapted for automated library preparation, offering greater convenience. The kit includes all necessary reagents for RNA fragmentation, reverse transcription, strand-specific ds-cDNA synthesis, and library amplification. It is compatible with upstream workflows involving mRNA enrichment or rRNA depletion. Engineered for enhanced user safety and data quality, the kit features an optimized reverse transcription system that achieves high strand specificity without the use of actinomycin D. Every component undergoes stringent quality control and functional testing to ensure robust library prep performance, batch-to-batch consistency, and high reproducibility. Specifications Cat.No. N210104S / N210104M / N210104MP Size 24 T / 96 T / 96 T (plate) Components Components No. Name N210104S N210104M N210104MP** N210104-A Frag/Primer Buffer 450 μL 2×900 μL 8×266 μL N210104-B 1st Reaction Module 2.0 192 μL 768 μL 8×120 μL N210104-C 2nd Reaction Module(dUTP) 840 μL 3×1120 μL 8×480 μL N210104-D Ligation Reaction Module 840 μL 3×1120 μL 8×480 μL N210104-E 2×Super CanaceTM II High-Fidelity Mix 600 μL 2×1200 μL 8×340 μL * Primer Mix* / / / [Note]:  * This component is not included in the kit. It is not required when using short or incomplete adapters. However, if full-length or complete adapters are used during library preparation, this component must be added separately. This kit is compatible with both IlluminaTM and MGITM platforms. Platform-specific primer mixes must be purchased separately: Cat# N210782 — RNA Library Prep Primer Mix for MGITM (compatible with complete adapters) Cat# N210701 — Primer Mix for IlluminaTM ** For the layout of reagents in the pre-loaded plate format, please refer to the figure below. The left image shows the reagent layout of the pre-loaded plate format library preparation kit, with each component occupying one column, and each well containing sufficient reagent for 12 library preparation reactions. When using the pre-loaded plate kit with automated instruments, the empty plate positions (columns 6-12) can be filled with beads, adapters, or other reagents according to experimental needs prior to the experiment. It is recommended that each well of the pre-loaded plate format reagent be used up in a single use.  Figure 1. Layout of Reagent Components for Plate-based Library Preparation Kit Storage This product should be stored at -25~-15℃ for 1 year. Documents： Manual Related Blog: The Ultimate Guide to NGS mRNA Library Prep: From mRNA Purification to Key Tips

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NGS Total RNA Library Prep Kit is a total RNA sequencing library preparation kit compatible with both Illumina and MGI platforms. It includes reagents for RNA fragmentation, reverse transcription, conventional and strand-specific double-stranded cDNA (ds-cDNA) synthesis, and library amplification. It can be used in conjunction with mRNA purification kits or rRNA depletion kits to prepare sequencing libraries. The second-strand synthesis module contains two types of buffers, allowing users to choose between conventional or strand-specific library preparation. In the strand-specific second-strand synthesis buffer, dTTP is replaced with dUTP, enabling dUTP incorporation into the second cDNA strand. The high-fidelity DNA polymerase used in this kit cannot amplify DNA templates containing uracil, thereby achieving strand specificity. All reagents included in the kit have undergone rigorous quality control and functional validation to ensure maximum library preparation stability and reproducibility. Specifications Cat.No. N210102S / N210102M Size 24 T / 96 T Components Components No. Name N210102S N210102M N210102-A 2× Frag/Prime Buffe 250 μL 930 μL N210102-B 1st Strand Enzyme Mix 48 μL 192 μL N210102-C Strand Specificity Reagent 150 μL 580 μL N210102-D 2nd Strand Buffer (dNTP) 720 μL 2×1440 μL N210102-E 2nd Strand Buffer (dUTP) 720 μL 2×1440 μL N210102-F 2nd Strand Enzyme Master Mix 120 μL 480 μL N210102-G Ligation Enhancer 720 μL 2×1440 μL N210102-H Novel T4 DNA Ligase 120 μL 480 μL N210102-I 2 × Super CanaceTM II High-Fidelity Mix 600 μL 2×1200 μL N210102-K Nuclease Free H2O 300 μL 1000 μL N210102-* Primer mix NA NA 【Note】: When using short adapters(Incomplete adapter), no primer mix is required. However, for Full-length adapters, a primer mix is essential and must be purchased separately. This kit is compatible with both Illumina™ and MGI™ platforms, but requires platform-specific primer mixes: Cat# N210701 for Illumina™; Cat# N210781 for MGI™. Storage This product should be stored at -25~-15℃ for 1 year. Documents： Manual

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DNA Fragmentation Reagent for ONT is a library preparation kit specifically designed for third-generation Nanopore sequencing platforms. This kit uses high-quality fragmentation enzymes, eliminating the need for cumbersome ultrasonication processes and simplifying the workflow by combining the fragmentation module with end-repair and dA-tailing modules. It significantly reduces the time and cost of library preparation. Suitable for 50 ng to 1 μg of genomic DNA from common animals, plants, microorganisms, etc., it enables DNA fragmentation, end repair, and A-tailing in a single tube. Specifications Cat.No. N210008E / N210008S / N210008M Size 8 T / 24 T / 96 T Components Components No. Name N210008E N210008S N210008M N210008-A SmearaseTM Buffer 80 μL 240 μL 960 μL N210008-B SmearaseTM Enzyme 40 μL 120 μL 480 μL Storage This product should be stored at -25~-15℃ for 1 year. Documents： Manual

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The DNA Library Prep Kit for ONT is designed for third-generation Nanopore sequencing platforms. This kit features optimized enzymes and buffers that significantly enhance the adapter ligation efficiency of long fragments. Furthermore, after end-repair and A-tailing, no purification is needed before proceeding directly to barcode ligation, streamlining the workflow. Suitable for 100 ng - 1.5 μg gDNA or amplicons. Library preparation time is approximately 2 hours. Rigorous batch performance and stability quality control measures are in place. Specifications Cat.No. N210007S / N210007M Size 24 T / 96 T Components Components No. Name N210007S N210007M N210007-A Endprep Buffer 168 μL 672 μL N210007-B Endprep Enzyme 72 μL 288 μL N210007-C Rapid Ligase Master Mix 840 μL 4×840 μL N210007-D Rapid Ligation Reaction Buffer（5×） 480 μL 2×960 μL N210007-E Rapid T4 DNA Ligase 120 μL 480 μL N210007-F Elution Buffer 480 μL 2×960 μL Storage This product should be stored at -25~-15℃ for 1 year. Workflow Documents： Manual

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NGS DNA Library Prep Kit (Mechanical) is a next-generation library preparation kit specifically developed for IlluminaTM and MGITM high-throughput sequencing platforms. This product utilizes high-quality enzymatic components, improving upon its predecessor by enhancing the efficiency of DNA fragment end repair and A-tailing, as well as optimizing adapter ligation efficiency. The kit employs a novel high-fidelity enzyme, significantly boosting amplification uniformity and fidelity. It can be applied to various samples including routine plant and animal genomes, microbial genomes, FFPE, cfDNA, ChIP DNA, and more, aiding in obtaining excellent sequencing data. Applicable to all DNA samples ranging from 100 pg to 1 μg, including cfDNA and FFPE. Offers high conversion efficiency, achieving library conversion rates above 70%. Verified across multiple samples for excellent library and sequencing outcomes. Undergoes stringent batch performance and stability quality control. Specifications Cat.No. N210005E / N210005S / N210005M Size 8 T / 24 T / 96 T Components Components No. Name N210001E N210001S N210001M N210005-A Endprep Buffer 2.0  56 μL 168 μL 672 μL N210005-B Endprep Enzyme 2.0  24 μL 72 μL 288 μL N210005-C Ligation Enhancer 2.0  240 μL 720 μL 3×960 μL N210005-D Rapid T4 DNA Ligase 2.0 80 μL 240 μL 2×480 μL N210005-E CanaceTM Pro Amplification Mix   200 μL 600 μL 3×800 μL 【Note】: When using short adapters(Incomplete adapter), no primer mix is required. However, for Full-length adapters, a primer mix is essential and must be purchased separately. This kit is compatible with both Illumina™ and MGI™ platforms, but requires platform-specific primer mixes: Cat# N210701 for Illumina™; Cat# N210781 for MGI™. Storage This product should be stored at -25~-15℃ for 1 year. Workflow Documents： Manual

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The NGS Flash DNA Library Prep Kit(Enzymatic) is a fast enzymatic library preparation kit. This product utilizes high-quality fragmentation enzymes that integrate the fragmentation with the end repair & A-tailing module, streamlining the workflow and significantly reducing the time and cost of library preparation. It is suitable for input amounts of 100 pg–500 ng of conventional plant and animal genomic DNA, microbial genomic DNA, etc., and allows rapid fragmentation, end repair, and A-tailing reactions to be completed in a single tube. The kit is compatible with both IlluminaTM and MGITM adapters and primers for sequencing on both platforms. Suitable for 100 pg–500 ng genomic DNA samples Compatible with IlluminaTM and MGITM high-throughput sequencing platforms Fragmentation and end repair completed within 5 minutes High library conversion rate and amplification efficiency Specifications Cat.No. N210004S / N210001 M Size 24 T / 96 T Components Components No. Name N210004E N210004S N210004-A SmearaseTM Mix 240 μL 960 μL N210004-B Ligation Enhancer 720 μL 4×720 μL N210004-C Fast T4 DNA Ligase 120 μL 480 μL N210004-D 2×Ultima HF Amplification Mix 600 μL 4×600 μL * Primer Mix* 120 μL 480 μL 【Note】: When using short adapters(Incomplete adapter), no primer mix is required. However, for Full-length adapters, a primer mix is essential and must be purchased separately. This kit is compatible with both Illumina™ and MGI™ platforms, but requires platform-specific primer mixes: Cat# N210701 for Illumina™; Cat# N210781 for MGI™. Storage This product should be stored at -25~-15℃ for 1 year. Workflow Documents： Manual

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The NGS DNA Library Prep Kit (Enzymatic) is a next-generation enzymatic library preparation kit compatible with both IlluminaTM and MGITM high-throughput sequencing platforms. Compared to traditional methods, this kit eliminates the need for cumbersome sonication by using high-quality fragmentation enzymes. The fragmentation and end-repair with dA-tailing modules are combined into a single step, significantly reducing the time and cost of library preparation. It is suitable for input amounts ranging from 1 ng to 1 μg of standard plant and animal genomic DNA, microbial genomic DNA, etc. The enzyme produces uniform fragmentation across different species with minimal species-specific variation. DNA fragmentation, end-repair and dA-tailing are all performed in a single tube. The kit is compatible with both IlluminaTM and MGITM adapters and primers for sequencing on both platforms. Applicable to genomic DNA and full-length cDNA input amounts of 1 ng to 1 μg High-quality fragmentation enzyme enables random dsDNA cleavage with minimal bias Fragmentation, end repair, and dA-tailing in a single step High-fidelity enzyme with strong amplification efficiency significantly improves library quality and yield Suitable for a wide range of species Strict batch performance and stability control Specifications Cat.No. N210001E / N210001S / N210001M /N210001L Size 8 T / 24 T / 96 T /96 T(Plate-Packaging） Components Components No. Name N210001E N210001S N210001M N210001L* N210001-A SmearaseTM Buffer 4.0 80 μL 240 μL 960 μL 8×150 μL N210001-B SmearaseTM Enzyme 4.0 80 μL 240 μL 960 μL 8×150 μL N210001-C Ligation Enhancer 4.0 240 μL 720 μL 3×960 μL 8×420 μL N210001-D Rapid DNA Ligase 4.0 80 μL 240 μL 2×480 μL 8×140 μL N210001-E 2×Ultima HF Amplification Mix 200 μL 600 μL 3×800 μL 8×340 μL 【Note】: When using incomplete adapters, no primer mix is required. However, for full-length adapters, a primer mix is essential and must be purchased separately. This kit is compatible with both Illumina™ and MGI™ platforms, but requires platform-specific primer mixes: Cat# N210701 for Illumina™; Cat# N210781 for MGI™. *For plate format (automated 96T), refer to the layout diagram below:      Storage This product should be stored at -25~-15℃ for 1 year. Workflow Documents： Manual Related Blog: Enzymatic Fragmentation DNA Library Prep Kits: Empowering Multi-Disciplinary Research Applications

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