Description
T4 DNA Polymerase catalyzes the synthesis of DNA in a 5'→3' direction in the presence of a template and primer. It possesses 3'→5' exonuclease activity but lacks 5'→3' exonuclease activity.
Specifications
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Cat.No.
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N210507E / N210507S / N210507M / N210507L
|
|
Size
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100 U / 500 U / 2000 U / 5000 U
|
Components
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Components No.
|
Name
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N210507E
|
N210507S
|
N210507M
|
N210507L
|
|
N210507-A
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T4 DNA polymerase (5 U/μL)
|
20 μL
|
100 μL
|
400 μL
|
1 mL
|
|
N210507-B
|
10 × Blue Buffer
|
100 μL
|
250 μL
|
500 μL
|
1.25 mL
|
Unit Definition
One unit (U) of enzyme is defined as the amount required to catalyze the incorporation of 10 nmol of deoxyribonucleotides (dNTPs) into polynucleotides in 30 minutes at 37°C.
Product Applications
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Blunting of DNA 5' or 3' overhangs;
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Synthesis of labeled DNA probes through displacement reactions;
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Second strand synthesis during site-directed mutagenesis;
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PCR product cloning without the need for ligation reactions.
Storage
Shipped with dry ice.This product should be stored at -25℃ ~-15℃ for 1 year.
Notes
1. For your safety and health, wear lab coats and disposable gloves during operation.
2. This product is for research use only!
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