NGS module

Product description NGS Library Amplification Mix for Library Amplification is a ready-to-use 2× premixed solution containing High-Fidelity DNA Polymerase (with fidelity six times higher than ordinary pfu DNA polymerase and an amplification speed of 15 sec/kb), dNTPs, and a buffer system meticulously optimized for high-throughput sequencing library amplification. It offers rapid and simple operation, high sensitivity, strong specificity, and excellent stability. During the library amplification reaction, only primers and templates need to be added to the mix, simplifying experimental steps, reducing human error, and improving experimental throughput and reproducibility. Additionally, this product contains a special stabilizer that maintains activity even after repeated freeze-thaw cycles. This product has been validated through successful use with the NGS End repair and dA-Tailing Module(Cat#N210417) and NGS Novel DNA Ligation Module(Cat#N210426) for DNA library construction, followed by sequencing on the IlluminaTM high-throughput platform. All reagents provided in this product undergo strict quality control to ensure optimal performance and batch-to-batch consistency. Specifications Cat.No. N210501S / N210501M Size 24 T / 96 T Product Applications Gene cloning; amplification of complex DNA templates; high-throughput library amplification. Storage Shipped with ice packs.This product should be stored at -25℃~-15℃ for 18 months. Notes 1. For your safety and health, wear lab coats and disposable gloves during operation. 2. This product is for research use only! Documents： Manual

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Product description NGS cDNA Synthesis Module (gDNA digester plus) is a cDNA synthesis reagent kit for RNA sequencing library preparation compatible with IlluminaTM/MGITM sequencing platforms. It includes reagents for gDNA removal, RNA reverse transcription, and ds-cDNA synthesis. All reagents in this kit have undergone rigorous quality control and functional validation to ensure maximum stability and reproducibility in library preparation. Specifications Cat.No. N210425E / N210425S / N210425M Size 8 T / 24 T / 96 T Components Components No. Name N210425E N210425S N210425M N210425-A DNase Ⅰ Buffer 16 μL 48 μL 192 μL N210425-B DNase I Mix 16 μL 48 μL 192 μL N210425-C 1st Reaction Buffer 48 μL 144 μL 580 μL N210425-D 1st Stand Enzyme Mix 16 μL 48 μL 192 μL N210425-E 2nd Reaction Buffer 56 μL 168 μL 672 μL N210425-F 2nd Strand Enzyme Mix 24 μL 72 μL 288 μL Storage This product should be stored at -25~-15℃ for 1 year. Documents： Manual

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Product description NGS cDNA Synthesis Module is a cDNA synthesis reagent kit for RNA sequencing library preparation compatible with IlluminaTM/MGITM sequencing platforms. It includes highly efficient reagents for RNA reverse transcription and standard double-stranded (ds) cDNA synthesis. The resulting ds-cDNA can be directly used with NGS Flash DNA Library Prep Kit (Enzymatic)(Arcegen Cat#N210004) for downstream library preparation. All reagents in this kit have undergone rigorous quality control and functional validation to ensure maximum stability and reproducibility in library preparation. Specifications Cat.No. N210423E / N210423S / N210423M Size 8 T / 24 T / 96 T Components Components No. Name N210423E N210423S N210423M N210423-A Random Primer 8 μL 24 μL 212 μL N210423-B 1st Reaction Buffer 64 μL 192 μL 1267 μL N210423-C 1st Strand Enzyme Mix 16 μL 48 μL 320 μL N210423-D 2nd Reaction Buffer 56 μL 168 μL 1034 μL N210423-E 2nd Strand Enzyme Mix 24 μL 72 μL 473 μL Storage This product should be stored at -25~-15℃ for 1 year. Documents： Manual

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Product description NGS Single Barcode Fast-Pace DNA Cyclization Kit for MGI is a single-stranded DNA cyclization kit specifically designed for the MGI high-throughput sequencing platform. Composed of high-quality enzymes and an optimized buffer system, this product significantly enhances reaction efficiency, enabling the entire cyclization and digestion process to be completed within 30 minutes. This kit is suitable for amplification products of all single-index PCR libraries constructed according to BGI's standard protocols. Except for limitations imposed by the library construction reagents themselves, this cyclization kit is not restricted to any specific MGI sequencing platform. Specifications Cat.No. N210421S / N210421M Size 16 T / 96 T Components Components No. Name N210421S N210421M N210421-A Splint Oligo 96 μL 576 μL N210421-B Splint Buffer 240 μL 2×720 μL N210421-C Ligase 80 μL 480 μL N210421-D Digestion Buffer 128 μL 768 μL N210421-E Digestion Enzyme 32 μL 192 μL Storage This product should be stored at -25~-15℃ for 1 year. Documents： Manual

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Product description NGS Dual Barcode Fast-Pace DNA Cyclization Kit for MGI is a single-stranded DNA cyclization kit specifically developed and designed for the MGI high-throughput sequencing platform. Composed of high-quality enzymes and an optimized buffer system, this product significantly improves reaction efficiency, enabling the entire cyclization and digestion process to be completed within 30 minutes. This kit is suitable for all PCR-amplified library products with dual barcodes connected according to BGI's standard. Except for limitations of the library construction reagents themselves, this cyclization kit is compatible with all MGI sequencing platforms. Specifications Cat.No. N210420S / N210420M Size 16 T / 96 T Components Components No. Name N210420S N210420M N210420-A DB Splint Oligo 96 μL 576 μL N210420-B Splint Buffer 240 μL 2×720 μL N210420-C Ligase 80 μL 480 μL N210420-D Digestion Buffer 128 μL 768 μL N210420-E Digestion Enzyme 32 μL 192 μL Storage This product should be stored at -25~-15℃ for 1 year. Documents： Manual

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Product description NGS Uracil+ Library Amplification Mix is a ready-to-use 2× premix solution containing Hieff CanaceTM Uracil+ High-Fidelity DNA polymerase, dNTPs, and an optimized buffer system specifically tailored for high-throughput sequencing library amplification. It offers high sensitivity, strong specificity, and excellent fidelity. During amplification reactions, users only need to add primers and template to the mixture, simplifying experimental procedures, reducing human error, and improving throughput and reproducibility of results. All reagents are rigorously quality-controlled to ensure superior performance and batch-to-batch consistency. Specifications Cat.No. N210419E / N210419S / N210419M Size 1 mL / 5 mL / 25 mL Storage This product should be stored at -25~-15℃ for 18 months. Documents： Manual

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Product description The NGS Fragmentation, End repair and dA-Tailing Module is a next-generation enzyme-based library preparation kit designed for high-throughput sequencing platforms from IlluminaTM and MGITM. This product utilizes high-quality fragmentation enzymes to eliminate the cumbersome ultrasonication process, simplifying the operation procedure by combining the fragmentation module with end-repair/dA-tailing into one. It significantly reduces library preparation time and costs. Suitable for 500 pg-1 μg of typical plant and animal genomic DNA, microbial genomes, etc., it enables DNA fragmentation, end repair, and dA tailing reactions in a single tube. The reaction products can be directly used for adapter ligation using the NGS Novel DNA Ligation Module(Cat#N210426). Specifications Cat.No. N210418S / N210418M Size 24 T / 96 T Components Components No. Name N210418S N210418M N210418-A SmearaseTM Buffer 240 μL 960 μL N210418-B SmearaseTM Enzyme 120 μL 480 μL Storage Shipped with dry ice.This product should be stored at -25~-15℃ for 18 months. Documents： Manual

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Product description NGS FFPE DNA Repair Reagent is a specialized repair solution designed for FFPE samples, capable of repairing various degrees of damage in low-quality FFPE samples, thereby improving library yield and sequencing quality. This product can be directly used with Arcegen NGS DNA Library Preparation Kits (Mechanical) (Cat#N210005) and the A-tailing module (Cat#N210417). The system is fully compatible and suitable for repairing 5 ng – 1 μg of fragmented FFPE DNA. Additionally, this product is also compatible with the Arcegen enzymatic fragmentation-based NGS DNA Library Prep Kit (Enzymatic)(Cat#N210001).  Specifications Cat.No. N210416S / N210416M Size 24 T / 96 T Components Components No. Name N210416S N210416M N210416-A FFPE DNA Repair Mix 96 μL 384 μL N210416-B Endprep Buffer 2.0 144 μL 576 μL Storage This product should be stored at -25~-15℃ for 2 years. Notes 1. For your safety and health, wear a lab coat and disposable gloves when handling this product. 2. Place all kit components on ice for thawing before use. After thawing, invert the tubes several times to ensure thorough mixing, briefly centrifuge, and then keep on ice until use. 3. When preparing reaction mixtures, gently pipette or vortex to mix. Avoid vigorous vortexing, which may reduce library yield. 4. To prevent cross-contamination between samples, use filter tips and change pipette tips when handling different samples. 5. It is recommended to perform all reaction steps in a thermal cycler with a heated lid. Pre-warm the thermal cycler to the reaction temperature prior to use. 6. This product is intended for research use only! Documents： Manual

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