DNA Library Prep

NGS PreMix RNA Library Prep Kit(dNTP) is a total RNA sequencing library preparation kit compatible with both IlluminaTM and MGITM platforms. This product is available in standard tube format and pre-loaded plate format. The pre-loaded plate format eliminates the need for manual reagent preparation or aliquoting, and can be directly adapted for automated library preparation, offering greater convenience. The kit includes all necessary reagents for RNA fragmentation, reverse transcription, strand-specific ds-cDNA synthesis, and library amplification. It is compatible with upstream workflows involving mRNA enrichment or rRNA depletion. Engineered for enhanced user safety and data quality, the kit features an optimized reverse transcription system that achieves high strand specificity without the use of actinomycin D. Every component undergoes stringent quality control and functional testing to ensure robust library prep performance, batch-to-batch consistency, and high reproducibility. Specifications Cat.No. N210105S / N210105M / N210105MP Size 24 T / 96 T / 96 T (plate) Components Components No. Name N210105S N210105M N210105MP** N210105-A Frag/Primer Buffer 450 μL 2×900 μL 8×266 μL N210105-B 1st Reaction Module 2.0 192 μL 768 μL 8×120 μL N210105-C 2nd Reaction Module(dNTP) 840 μL 3×1120 μL 8×480 μL N210105-D Ligation Reaction Module 840 μL 3×1120 μL 8×480 μL N210105-E 2×Super CanaceTM II High-Fidelity Mix 600 μL 2×1200 μL 8×340 μL * Primer Mix* / / / [Note]: * This component is not included in the kit. It is not required when using short or incomplete adapters. However, if full-length or complete adapters are used during library preparation, this component must be added separately. This kit is compatible with both IlluminaTM and MGITM platforms. Platform-specific Primer mixes must be purchased separately: Cat# N210782 — RNA Library Prep Primer Mix for MGITM (compatible with complete adapters) Cat# N210701 — Primer Mix for IlluminaTM ** For the layout of reagents in the pre-loaded plate format, please refer to the figure below. The left image shows the reagent layout of the pre-loaded plate format library preparation kit, with each component occupying one column, and each well containing sufficient reagent for 12 library preparation reactions. When using the pre-loaded plate kit with automated instruments, the empty plate positions (columns 6-12) can be filled with beads, adapters, or other reagents according to experimental needs prior to the experiment. It is recommended that each well of the pre-loaded plate format reagent be used up in a single use.  Figure 1. Layout of Reagent Components for Plate-based Library Preparation Kit Storage This product should be stored at -25~-15℃ for 1 year. Documents： Manual

Read more less

NGS PreMix RNA Library Prep Kit(Strand-specific) is a strand-specific RNA sequencing library preparation kit compatible with both IlluminaTM and MGITM platforms. This product is available in standard tube format and pre-loaded plate format. The pre-loaded plate format eliminates the need for manual reagent preparation or aliquoting, and can be directly adapted for automated library preparation, offering greater convenience. The kit includes all necessary reagents for RNA fragmentation, reverse transcription, strand-specific ds-cDNA synthesis, and library amplification. It is compatible with upstream workflows involving mRNA enrichment or rRNA depletion. Engineered for enhanced user safety and data quality, the kit features an optimized reverse transcription system that achieves high strand specificity without the use of actinomycin D. Every component undergoes stringent quality control and functional testing to ensure robust library prep performance, batch-to-batch consistency, and high reproducibility. Specifications Cat.No. N210104S / N210104M / N210104MP Size 24 T / 96 T / 96 T (plate) Components Components No. Name N210104S N210104M N210104MP** N210104-A Frag/Primer Buffer 450 μL 2×900 μL 8×266 μL N210104-B 1st Reaction Module 2.0 192 μL 768 μL 8×120 μL N210104-C 2nd Reaction Module(dUTP) 840 μL 3×1120 μL 8×480 μL N210104-D Ligation Reaction Module 840 μL 3×1120 μL 8×480 μL N210104-E 2×Super CanaceTM II High-Fidelity Mix 600 μL 2×1200 μL 8×340 μL * Primer Mix* / / / [Note]:  * This component is not included in the kit. It is not required when using short or incomplete adapters. However, if full-length or complete adapters are used during library preparation, this component must be added separately. This kit is compatible with both IlluminaTM and MGITM platforms. Platform-specific primer mixes must be purchased separately: Cat# N210782 — RNA Library Prep Primer Mix for MGITM (compatible with complete adapters) Cat# N210701 — Primer Mix for IlluminaTM ** For the layout of reagents in the pre-loaded plate format, please refer to the figure below. The left image shows the reagent layout of the pre-loaded plate format library preparation kit, with each component occupying one column, and each well containing sufficient reagent for 12 library preparation reactions. When using the pre-loaded plate kit with automated instruments, the empty plate positions (columns 6-12) can be filled with beads, adapters, or other reagents according to experimental needs prior to the experiment. It is recommended that each well of the pre-loaded plate format reagent be used up in a single use.  Figure 1. Layout of Reagent Components for Plate-based Library Preparation Kit Storage This product should be stored at -25~-15℃ for 1 year. Documents： Manual

Read more less

NGS Total RNA Library Prep Kit is a total RNA sequencing library preparation kit compatible with both Illumina and MGI platforms. It includes reagents for RNA fragmentation, reverse transcription, conventional and strand-specific double-stranded cDNA (ds-cDNA) synthesis, and library amplification. It can be used in conjunction with mRNA purification kits or rRNA depletion kits to prepare sequencing libraries. The second-strand synthesis module contains two types of buffers, allowing users to choose between conventional or strand-specific library preparation. In the strand-specific second-strand synthesis buffer, dTTP is replaced with dUTP, enabling dUTP incorporation into the second cDNA strand. The high-fidelity DNA polymerase used in this kit cannot amplify DNA templates containing uracil, thereby achieving strand specificity. All reagents included in the kit have undergone rigorous quality control and functional validation to ensure maximum library preparation stability and reproducibility. Specifications Cat.No. N210102S / N210102M Size 24 T / 96 T Components Components No. Name N210102S N210102M N210102-A 2× Frag/Prime Buffe 250 μL 930 μL N210102-B 1st Strand Enzyme Mix 48 μL 192 μL N210102-C Strand Specificity Reagent 150 μL 580 μL N210102-D 2nd Strand Buffer (dNTP) 720 μL 2×1440 μL N210102-E 2nd Strand Buffer (dUTP) 720 μL 2×1440 μL N210102-F 2nd Strand Enzyme Master Mix 120 μL 480 μL N210102-G Ligation Enhancer 720 μL 2×1440 μL N210102-H Novel T4 DNA Ligase 120 μL 480 μL N210102-I 2 × Super CanaceTM II High-Fidelity Mix 600 μL 2×1200 μL N210102-K Nuclease Free H2O 300 μL 1000 μL N210102-* Primer mix NA NA 【Note】: When using short adapters(Incomplete adapter), no primer mix is required. However, for Full-length adapters, a primer mix is essential and must be purchased separately. This kit is compatible with both Illumina™ and MGI™ platforms, but requires platform-specific primer mixes: Cat# N210701 for Illumina™; Cat# N210781 for MGI™. Storage This product should be stored at -25~-15℃ for 1 year. Documents： Manual

Read more less

DNA Fragmentation Reagent for ONT is a library preparation kit specifically designed for third-generation Nanopore sequencing platforms. This kit uses high-quality fragmentation enzymes, eliminating the need for cumbersome ultrasonication processes and simplifying the workflow by combining the fragmentation module with end-repair and dA-tailing modules. It significantly reduces the time and cost of library preparation. Suitable for 50 ng to 1 μg of genomic DNA from common animals, plants, microorganisms, etc., it enables DNA fragmentation, end repair, and A-tailing in a single tube. Specifications Cat.No. N210008E / N210008S / N210008M Size 8 T / 24 T / 96 T Components Components No. Name N210008E N210008S N210008M N210008-A SmearaseTM Buffer 80 μL 240 μL 960 μL N210008-B SmearaseTM Enzyme 40 μL 120 μL 480 μL Storage This product should be stored at -25~-15℃ for 1 year. Documents： Manual

Read more less

The DNA Library Prep Kit for ONT is designed for third-generation Nanopore sequencing platforms. This kit features optimized enzymes and buffers that significantly enhance the adapter ligation efficiency of long fragments. Furthermore, after end-repair and A-tailing, no purification is needed before proceeding directly to barcode ligation, streamlining the workflow. Suitable for 100 ng - 1.5 μg gDNA or amplicons. Library preparation time is approximately 2 hours. Rigorous batch performance and stability quality control measures are in place. Specifications Cat.No. N210007S / N210007M Size 24 T / 96 T Components Components No. Name N210007S N210007M N210007-A Endprep Buffer 168 μL 672 μL N210007-B Endprep Enzyme 72 μL 288 μL N210007-C Rapid Ligase Master Mix 840 μL 4×840 μL N210007-D Rapid Ligation Reaction Buffer（5×） 480 μL 2×960 μL N210007-E Rapid T4 DNA Ligase 120 μL 480 μL N210007-F Elution Buffer 480 μL 2×960 μL Storage This product should be stored at -25~-15℃ for 1 year. Workflow Documents： Manual

Read more less

NGS DNA Library Prep Kit (Mechanical) is a next-generation library preparation kit specifically developed for IlluminaTM and MGITM high-throughput sequencing platforms. This product utilizes high-quality enzymatic components, improving upon its predecessor by enhancing the efficiency of DNA fragment end repair and A-tailing, as well as optimizing adapter ligation efficiency. The kit employs a novel high-fidelity enzyme, significantly boosting amplification uniformity and fidelity. It can be applied to various samples including routine plant and animal genomes, microbial genomes, FFPE, cfDNA, ChIP DNA, and more, aiding in obtaining excellent sequencing data. Applicable to all DNA samples ranging from 100 pg to 1 μg, including cfDNA and FFPE. Offers high conversion efficiency, achieving library conversion rates above 70%. Verified across multiple samples for excellent library and sequencing outcomes. Undergoes stringent batch performance and stability quality control. Specifications Cat.No. N210005E / N210005S / N210005M Size 8 T / 24 T / 96 T Components Components No. Name N210001E N210001S N210001M N210005-A Endprep Buffer 2.0  56 μL 168 μL 672 μL N210005-B Endprep Enzyme 2.0  24 μL 72 μL 288 μL N210005-C Ligation Enhancer 2.0  240 μL 720 μL 3×960 μL N210005-D Rapid T4 DNA Ligase 2.0 80 μL 240 μL 2×480 μL N210005-E CanaceTM Pro Amplification Mix   200 μL 600 μL 3×800 μL 【Note】: When using short adapters(Incomplete adapter), no primer mix is required. However, for Full-length adapters, a primer mix is essential and must be purchased separately. This kit is compatible with both Illumina™ and MGI™ platforms, but requires platform-specific primer mixes: Cat# N210701 for Illumina™; Cat# N210781 for MGI™. Storage This product should be stored at -25~-15℃ for 1 year. Workflow Documents： Manual

Read more less

The NGS Flash DNA Library Prep Kit(Enzymatic) is a fast enzymatic library preparation kit. This product utilizes high-quality fragmentation enzymes that integrate the fragmentation with the end repair & A-tailing module, streamlining the workflow and significantly reducing the time and cost of library preparation. It is suitable for input amounts of 100 pg–500 ng of conventional plant and animal genomic DNA, microbial genomic DNA, etc., and allows rapid fragmentation, end repair, and A-tailing reactions to be completed in a single tube. The kit is compatible with both IlluminaTM and MGITM adapters and primers for sequencing on both platforms. Suitable for 100 pg–500 ng genomic DNA samples Compatible with IlluminaTM and MGITM high-throughput sequencing platforms Fragmentation and end repair completed within 5 minutes High library conversion rate and amplification efficiency Specifications Cat.No. N210004S / N210001 M Size 24 T / 96 T Components Components No. Name N210004E N210004S N210004-A SmearaseTM Mix 240 μL 960 μL N210004-B Ligation Enhancer 720 μL 4×720 μL N210004-C Fast T4 DNA Ligase 120 μL 480 μL N210004-D 2×Ultima HF Amplification Mix 600 μL 4×600 μL * Primer Mix* 120 μL 480 μL 【Note】: When using short adapters(Incomplete adapter), no primer mix is required. However, for Full-length adapters, a primer mix is essential and must be purchased separately. This kit is compatible with both Illumina™ and MGI™ platforms, but requires platform-specific primer mixes: Cat# N210701 for Illumina™; Cat# N210781 for MGI™. Storage This product should be stored at -25~-15℃ for 1 year. Workflow Documents： Manual

Read more less

The NGS DNA Library Prep Kit (Enzymatic) is a next-generation enzymatic library preparation kit compatible with both IlluminaTM and MGITM high-throughput sequencing platforms. Compared to traditional methods, this kit eliminates the need for cumbersome sonication by using high-quality fragmentation enzymes. The fragmentation and end-repair with dA-tailing modules are combined into a single step, significantly reducing the time and cost of library preparation. It is suitable for input amounts ranging from 1 ng to 1 μg of standard plant and animal genomic DNA, microbial genomic DNA, etc. The enzyme produces uniform fragmentation across different species with minimal species-specific variation. DNA fragmentation, end-repair and dA-tailing are all performed in a single tube. The kit is compatible with both IlluminaTM and MGITM adapters and primers for sequencing on both platforms. Applicable to genomic DNA and full-length cDNA input amounts of 1 ng to 1 μg High-quality fragmentation enzyme enables random dsDNA cleavage with minimal bias Fragmentation, end repair, and dA-tailing in a single step High-fidelity enzyme with strong amplification efficiency significantly improves library quality and yield Suitable for a wide range of species Strict batch performance and stability control Specifications Cat.No. N210001E / N210001S / N210001M /N210001L Size 8 T / 24 T / 96 T /96 T(Plate-Packaging） Components Components No. Name N210001E N210001S N210001M N210001L* N210001-A SmearaseTM Buffer 4.0 80 μL 240 μL 960 μL 8×150 μL N210001-B SmearaseTM Enzyme 4.0 80 μL 240 μL 960 μL 8×150 μL N210001-C Ligation Enhancer 4.0 240 μL 720 μL 3×960 μL 8×420 μL N210001-D Rapid DNA Ligase 4.0 80 μL 240 μL 2×480 μL 8×140 μL N210001-E 2×Ultima HF Amplification Mix 200 μL 600 μL 3×800 μL 8×340 μL 【Note】: When using incomplete adapters, no primer mix is required. However, for full-length adapters, a primer mix is essential and must be purchased separately. This kit is compatible with both Illumina™ and MGI™ platforms, but requires platform-specific primer mixes: Cat# N210701 for Illumina™; Cat# N210781 for MGI™. *For plate format (automated 96T), refer to the layout diagram below:      Storage This product should be stored at -25~-15℃ for 1 year. Workflow Documents： Manual

Read more less