RNA Library Prep

NGS Total RNA Library Prep Kit is a total RNA sequencing library preparation kit compatible with both Illumina and MGI platforms. It includes reagents for RNA fragmentation, reverse transcription, conventional and strand-specific double-stranded cDNA (ds-cDNA) synthesis, and library amplification. It can be used in conjunction with mRNA purification kits or rRNA depletion kits to prepare sequencing libraries. The second-strand synthesis module contains two types of buffers, allowing users to choose between conventional or strand-specific library preparation. In the strand-specific second-strand synthesis buffer, dTTP is replaced with dUTP, enabling dUTP incorporation into the second cDNA strand. The high-fidelity DNA polymerase used in this kit cannot amplify DNA templates containing uracil, thereby achieving strand specificity. All reagents included in the kit have undergone rigorous quality control and functional validation to ensure maximum library preparation stability and reproducibility. Specifications Cat.No. N210102S / N210102M Size 24 T / 96 T Components Components No. Name N210102S N210102M N210102-A 2× Frag/Prime Buffe 250 μL 930 μL N210102-B 1st Strand Enzyme Mix 48 μL 192 μL N210102-C Strand Specificity Reagent 150 μL 580 μL N210102-D 2nd Strand Buffer (dNTP) 720 μL 2×1440 μL N210102-E 2nd Strand Buffer (dUTP) 720 μL 2×1440 μL N210102-F 2nd Strand Enzyme Master Mix 120 μL 480 μL N210102-G Ligation Enhancer 720 μL 2×1440 μL N210102-H Novel T4 DNA Ligase 120 μL 480 μL N210102-I 2 × Super CanaceTM II High-Fidelity Mix 600 μL 2×1200 μL N210102-K Nuclease Free H2O 300 μL 1000 μL N210102-* Primer mix NA NA 【Note】: When using short adapters(Incomplete adapter), no primer mix is required. However, for Full-length adapters, a primer mix is essential and must be purchased separately. This kit is compatible with both Illumina™ and MGI™ platforms, but requires platform-specific primer mixes: Cat# N210701 for Illumina™; Cat# N210781 for MGI™. Storage This product should be stored at -25~-15℃ for 1 year. Documents： Manual

Read more less

NGS PreMix RNA Library Prep Kit(Strand-specific) is a strand-specific RNA sequencing library preparation kit compatible with both IlluminaTM and MGITM platforms. This product is available in standard tube format and pre-loaded plate format. The pre-loaded plate format eliminates the need for manual reagent preparation or aliquoting, and can be directly adapted for automated library preparation, offering greater convenience. The kit includes all necessary reagents for RNA fragmentation, reverse transcription, strand-specific ds-cDNA synthesis, and library amplification. It is compatible with upstream workflows involving mRNA enrichment or rRNA depletion. Engineered for enhanced user safety and data quality, the kit features an optimized reverse transcription system that achieves high strand specificity without the use of actinomycin D. Every component undergoes stringent quality control and functional testing to ensure robust library prep performance, batch-to-batch consistency, and high reproducibility. Specifications Cat.No. N210104S / N210104M / N210104MP Size 24 T / 96 T / 96 T (plate) Components Components No. Name N210104S N210104M N210104MP** N210104-A Frag/Primer Buffer 450 μL 2×900 μL 8×266 μL N210104-B 1st Reaction Module 2.0 192 μL 768 μL 8×120 μL N210104-C 2nd Reaction Module(dUTP) 840 μL 3×1120 μL 8×480 μL N210104-D Ligation Reaction Module 840 μL 3×1120 μL 8×480 μL N210104-E 2×Super CanaceTM II High-Fidelity Mix 600 μL 2×1200 μL 8×340 μL * Primer Mix* / / / [Note]:  * This component is not included in the kit. It is not required when using short or incomplete adapters. However, if full-length or complete adapters are used during library preparation, this component must be added separately. This kit is compatible with both IlluminaTM and MGITM platforms. Platform-specific primer mixes must be purchased separately: Cat# N210782 — RNA Library Prep Primer Mix for MGITM (compatible with complete adapters) Cat# N210701 — Primer Mix for IlluminaTM ** For the layout of reagents in the pre-loaded plate format, please refer to the figure below. The left image shows the reagent layout of the pre-loaded plate format library preparation kit, with each component occupying one column, and each well containing sufficient reagent for 12 library preparation reactions. When using the pre-loaded plate kit with automated instruments, the empty plate positions (columns 6-12) can be filled with beads, adapters, or other reagents according to experimental needs prior to the experiment. It is recommended that each well of the pre-loaded plate format reagent be used up in a single use.  Figure 1. Layout of Reagent Components for Plate-based Library Preparation Kit Storage This product should be stored at -25~-15℃ for 1 year. Documents： Manual Related Blog: The Ultimate Guide to NGS mRNA Library Prep: From mRNA Purification to Key Tips

Read more less

NGS PreMix RNA Library Prep Kit(dNTP) is a total RNA sequencing library preparation kit compatible with both IlluminaTM and MGITM platforms. This product is available in standard tube format and pre-loaded plate format. The pre-loaded plate format eliminates the need for manual reagent preparation or aliquoting, and can be directly adapted for automated library preparation, offering greater convenience. The kit includes all necessary reagents for RNA fragmentation, reverse transcription, strand-specific ds-cDNA synthesis, and library amplification. It is compatible with upstream workflows involving mRNA enrichment or rRNA depletion. Engineered for enhanced user safety and data quality, the kit features an optimized reverse transcription system that achieves high strand specificity without the use of actinomycin D. Every component undergoes stringent quality control and functional testing to ensure robust library prep performance, batch-to-batch consistency, and high reproducibility. Specifications Cat.No. N210105S / N210105M / N210105MP Size 24 T / 96 T / 96 T (plate) Components Components No. Name N210105S N210105M N210105MP** N210105-A Frag/Primer Buffer 450 μL 2×900 μL 8×266 μL N210105-B 1st Reaction Module 2.0 192 μL 768 μL 8×120 μL N210105-C 2nd Reaction Module(dNTP) 840 μL 3×1120 μL 8×480 μL N210105-D Ligation Reaction Module 840 μL 3×1120 μL 8×480 μL N210105-E 2×Super CanaceTM II High-Fidelity Mix 600 μL 2×1200 μL 8×340 μL * Primer Mix* / / / [Note]: * This component is not included in the kit. It is not required when using short or incomplete adapters. However, if full-length or complete adapters are used during library preparation, this component must be added separately. This kit is compatible with both IlluminaTM and MGITM platforms. Platform-specific Primer mixes must be purchased separately: Cat# N210782 — RNA Library Prep Primer Mix for MGITM (compatible with complete adapters) Cat# N210701 — Primer Mix for IlluminaTM ** For the layout of reagents in the pre-loaded plate format, please refer to the figure below. The left image shows the reagent layout of the pre-loaded plate format library preparation kit, with each component occupying one column, and each well containing sufficient reagent for 12 library preparation reactions. When using the pre-loaded plate kit with automated instruments, the empty plate positions (columns 6-12) can be filled with beads, adapters, or other reagents according to experimental needs prior to the experiment. It is recommended that each well of the pre-loaded plate format reagent be used up in a single use.  Figure 1. Layout of Reagent Components for Plate-based Library Preparation Kit Storage This product should be stored at -25~-15℃ for 1 year. Documents： Manual

Read more less