rRNA Depletion

Product description The Globin mRNA Depletion Probe (Human) is a probe specifically designed to target human globin mRNAs, enabling efficient removal of globin mRNA transcripts derived from adults, infants, and embryos, including HBA1/2, HBB, HBD, HBM, HBG1/2, HBE1, HBQ1, and HBZ. When used in combination with the NGS rRNA Depletion Kit (Human/Mouse/Rat) (Arcegen #N210402), this product effectively removes both rRNA and globin mRNA from total RNA while preserving other messenger RNAs (mRNAs) and non-coding RNAs. This kit performs well on both intact and partially degraded total RNA samples. The RNA samples obtained after rRNA & globin mRNA depletion can be used for high-throughput sequencing analysis of mRNA and non-coding RNA, significantly increasing the proportion of usable sequencing data. They may also be used for cDNA synthesis or other downstream applications. Application Scope Suitable for human-derived total RNA samples in the range of 100 ng to 1 μg; suitable for both intact and partially degraded RNA samples. Specifications Cat.No. N210404S / N210404M Size 24 T / 96 T Components Components NO. Name N210404S N210404M N210404 Globin Probe (Human) 24 μL 96 μL Storage Shipped on dry ice, store at –25°C ~ –15°C, Valid for 1 year. Notes 1. Use RNase-free consumables and regularly clean the experimental area. We recommend using Thermo Fisher’s RNAZap™ efficient nucleic acid removal spray to eliminate RNase contamination. 2. RNA samples must be free of genomic DNA contamination. If gDNA is present, perform DNase I digestion followed by purification before using this kit. 3. The maximum input volume for RNA samples is 10 μL. If the sample volume exceeds this, it should be concentrated first. 4. For safety and health, wear a lab coat and disposable gloves during operation. 5. This product is intended for research use only. Documents： Manual

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Product description NGS rRNA Depletion Kit ( Human/Mouse/Rat) utilizes an RNase H digestion method to remove ribosomal RNA (rRNA) and the 45S & ITS/ETS regions from total RNA isolated from human, mouse, and rat samples, thereby enriching messenger RNA (mRNA) and other non-coding RNAs. This kit effectively removes rRNA from both intact and partially degraded total RNA (such as FFPE-derived RNA). Since degraded FFPE samples typically contain a higher proportion of ITS/ETS fragments compared to fresh tissue samples, the inclusion of specific probes targeting the human, mouse, and rat 45S & ITS/ETS regions in this kit significantly increases the proportion of usable sequencing data after depletion. Specifications Cat NO. N210402E / N210402S / N210402M Size 12 T/24 T /96 T Components Components No. Name N210402E N210402S N210402M N210402-A Hybridization Buffer 36 μL 72 μL 288 μL N210402-B Human Probe Mix (rRNA & ITS/ETS) 24 μL 48 μL 192 μL N210402-C RNase H Buffer 36 μL 72 μL 288 μL N210402-D Thermostable RNase H 24 μL 48 μL 192 μL N210402-E DNase I Buffer 330 μL 660 μL 2×1320 μL N210402-F DNase l 30 μL 60 μL 240 μL Storage This product should be stored at -25~-15℃ for 1 year. Notes 1. Use RNase-free consumables and regularly clean work areas. It is recommended to use Thermo Fisher’s RNAZapTM efficient nucleic acid removal spray to eliminate RNase contamination. 2. RNA samples should be free of genomic DNA (gDNA) contamination. If gDNA is present, perform DNase I digestion followed by purification prior to using this kit. 3. The maximum input volume for RNA samples is 10 μL. If the sample volume exceeds this, concentrate the sample before use. 4. If preparing a mix for the RNase H digestion step, prepare it fresh and use immediately. 5. For your safety and health, wear a lab coat and disposable gloves during operation. 6. This product is intended for research use only! Documents： Manual

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.Product description NGS rRNA Depletion Kit (Plant) utilizes RNase H digestion to remove ribosomal RNA (including cytoplasmic 18S, 25S rRNA, mitochondrial 18S, 26S rRNA, and chloroplast 16S, 23S rRNA) from total RNA of plant origin, thereby preserving messenger RNA (mRNA) and other non-coding RNA. This kit provides effective rRNA removal for both intact and partially degraded total RNA samples. The RNA samples obtained after rRNA depletion can be used for high-throughput sequencing to analyze mRNA and non-coding RNA, significantly increasing the proportion of usable sequencing data. They can also be used for cDNA synthesis or other downstream applications. Application Suitable for total RNA samples from various plant sources ranging from 100 ng to 1 μg; suitable for both intact and partially degraded RNA samples. Specifications Cat.No. N210401E / N210401S / N210401M Size 12 T / 24 T / 96 T Components Components No. Name N210401E N210401S N210401M N210401-A Hybridization Buffer 36 μL 72 μL 288 μL N210401-B Probe Mix (Plant) 36 μL 72μL 288 μL N210401-C RNase H Buffer 36 μL 72 μL 288 μL N210401-D Thermostable RNase H 24 μL 48 μL 192 μL N210401-E DNase I Buffer 330 μL 660 μL 2×1320 μL N210401-F DNase I 30 μL 60 μL 240 μL Storage Shipped on dry ice, store at -25~-15 °C.Valid for one year. Notes 1. Please use RNase-free consumables and regularly clean the work area. Thermo Fisher Scientific’s RNAZap™ High-Efficiency Nuclease Removal Spray is recommended for RNase contamination removal. 2. RNA samples should be free of genomic DNA contamination. If gDNA is present, perform DNase I digestion followed by purification before using this kit. 3. The maximum input volume for RNA samples is 10 μL. If the sample volume exceeds this, concentrate the RNA before use. 4. If the RNase H digestion step requires a reaction mix, please prepare it freshly before use. 5. For personal safety and health, wear a lab coat and disposable gloves during operation. 6. This product is for research use only. Documents： Manual

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