Mycoplasma Detection and Elimination

Product description DfCell Mycoplasma Off Spray effectively inhibits the growth and contamination of fungi (including spores), bacteria (including spores), mycoplasma, and viruses (including HIV and Hepatitis B). Its active ingredient is a quaternary ammonium derivative. It is free from mercury, formaldehyde, phenol, alcohol, and other irritants, non-toxic, and biodegradable. It does not cause any damage to general work surfaces and equipment. This product is a ready-to-use solution, to be used directly. Specifications Catalog Number C230101E/C230101S/C230101M Specifications 500 mL /2×500 mL/10×500 mL Storage Store and transport at room temperature. Shelf life is 18 months. Notes 1. Please read the entire instruction manual carefully before using this reagent. 2. Standardized operations, including the preparation of the reaction system, sample handling, and sample addition, should be followed throughout the experiment. 3. For your safety and health, wear laboratory coats and disposable gloves during operation. 4. For research use only. Instructions 1. Spray the CO2 incubator once every two weeks. There is no need to empty the incubator before spraying. 2. Spray the laminar flow hood before each use and allow it to air dry naturally. Documents: Manuals C230101-EN-Manual.pdf

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Product description Regular monitoring during cell culture is effective in preventing mycoplasma contamination. However, if cells are already contaminated with mycoplasma, prompt treatment is necessary. The optimal treatment approach involves high-pressure sterilization of contaminated cells followed by disposal to prevent contamination of other clean cell lines. If contaminated cells are valuable, mycoplasma contamination must be removed. Since mycoplasma lacks a cell wall, traditional antibiotics are generally ineffective against them. This product is an improved mixture specifically designed for mycoplasma removal. It achieves excellent mycoplasma clearance by inhibiting the synthesis of essential proteins required for DNA and mycoplasma growth. It is non-toxic to cells, thus maximizing the preservation of your valuable cells and minimizing losses caused by mycoplasma contamination. Specifications Catalog Number C230102S/C230102M Specifications 1 mL/5×1 mL Storage Transportation with Ice Packs. Store in a dark place at -20°C. Shelf life is 18 months. If not in use for an extended period, store in a dark place. Notes 1. Before using this reagent, please read the instruction manual carefully. 2. Standardized operations, including the preparation of the reaction system, sample handling, and sample addition, should be followed throughout the experiment. 3. For your safety and health, wear laboratory coats and disposable gloves during operation. 4. For research use only. Instructions 1. Before use, ensure the bottle cap is tightly sealed, thaw the solution to room temperature, gently vortex to mix thoroughly, and wipe the surface of the bottle with 70% ethanol before placing it in a laminar flow hood. 2. Follow the instructions below when using DfCell reagent to treat cells 3. After splitting contaminated cells, add 10 µL of DfCell reagent solution to 10 mL of culture medium and culture for 7 days. During this period, when changing the medium, add DfCell reagent in the same proportion. 4. After 7 days of cell culture, switch to a medium without mycoplasma elimination reagent and culture for 2 days. Take 10 µL of cell culture supernatant for mycoplasma detection to assess the clearance effect. 【Note】 a. Due to variations in cell tolerance to this product (in most cases, low cytotoxicity), if the product exhibits toxicity to cells or slows cell growth during use, it can be diluted at a ratio of 1:2000 before use. b. If mycoplasma contamination is severe and 7 days of treatment with the elimination reagent does not completely clear the mycoplasma, the treatment time can be extended appropriately. c. After treatment, it is recommended to use the DfCell Mycoplasma LAMP Detection Kit(Cat#C230105) or DfCell Mycoplasma RT-qPCR Detection Kit (Cat#C230106) to assess the removal effect. Documents: Manuals C230102-EN-Manual.pdf

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Product description The DfCell  Mycoplasma LAMP Detection Kit is a rapid detection product developed using Arcegen's unique isothermal amplification technology for detecting mycoplasma contamination in cell culture fluids. The main principle is that if cell cultures are contaminated with mycoplasma, the conserved sequence of mycoplasma DNA will be amplified rapidly and abundantly, causing the reaction mixture to change from blue-purple to sky blue, which can be visually distinguished without the need for electrophoresis. The DfCell Mycoplasma LAMP Detection Kit can detect multiple types of mycoplasma, including the 8 common mycoplasma strains commonly found in cell culture. Traditional nested PCR mycoplasma detection methods are prone to false negative results due to the presence of inhibitors in the cell culture supernatant and require opening the lid for electrophoresis after the reaction, increasing the risk of false positive results due to contamination. The DfCell LAMP Kit completely eliminates these drawbacks and has high sensitivity and accuracy.  Specifications Catalog Number C230105S/C230105M Specifications 25 T/100 T Components Component Number Component Name C230105S C230105M C230105-A LAMP Mix 600 μL 600 μL×4 C230105-B LAMP Primer 25 μL 25 μL×4 C230105-C Positive Control 10 μL 10 μL×4 C230105-D Mineral Oil 500 μL 500 μL×4 Storage Transportation with Ice Packs. Store in a dark place at -20°C. Shelf life is 18 months. If not in use for an extended period, please store in a dark place. Documents: Manuals C230105-EN-Manual.pdf  

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Product description DfCell Mycoplasma qPCR Detection Kit is a rapid qualitative test based on Nucleic Acid Amplification Techniques (NAT) designed to detect potential Mycoplasma contamination in raw materials, cell banks, virus seeds, virus or cell harvests, and therapeutic cell products. This assay kit utilizes quantitative PCR technology employing a multiplex PCR approach with two fluorescent probes, FAM and VIC, to respectively detect the target sequence and internal reference. It covers over 100 Mycoplasma DNA sequences and has undergone rigorous validation for specificity, detection limit, and durability according to EP 2.6.7 standards. The detection limit meets the requirement of ≤10 CFU/mL, demonstrating high sensitivity, excellent specificity, and safety. Specifications Product Number C230106E / C230106S Specifications 25 T / 100 T Components Component Number Component Name C230106E C230106S C230106-A 4×qPCR Reaction Buffer 250 μL 1 mL C230106-B Primer & Probe Mix 25 μL 100 μL C230106-C Internal Control (IC) 25 μL 100 μL C230106-D* Positive Control (PC) 500 μL 2 mL C230106-E** DNA Dilution Buffer 1 mL 4×1 mL C230106-F*** ddH2O 500 μL 2×1 mL *PC：Positive control solution, with a concentration of 1, 000 copies/µL; **DNA Dilution Buffer: Used for sample and IC dilution, as well as template for NTC and NC; ***ddH2O: Used for preparing qPCR Mix system. Storage Store at temperatures ranging from -15°C to -25°C with a shelf life of one year. Please note that component C230106-B should be stored protected from light. Documents: Manuals C230106-EN-Manual.pdf

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