Description
Product description
DfCell Mycoplasma qPCR Detection Kit is a rapid qualitative test based on Nucleic Acid Amplification Techniques (NAT) designed to detect potential Mycoplasma contamination in raw materials, cell banks, virus seeds, virus or cell harvests, and therapeutic cell products. This assay kit utilizes quantitative PCR technology employing a multiplex PCR approach with two fluorescent probes, FAM and VIC, to respectively detect the target sequence and internal reference. It covers over 100 Mycoplasma DNA sequences and has undergone rigorous validation for specificity, detection limit, and durability according to EP 2.6.7 standards. The detection limit meets the requirement of ≤10 CFU/mL, demonstrating high sensitivity, excellent specificity, and safety.
Specifications
|
Product Number
|
C230106E / C230106S
|
|
Specifications
|
25 T / 100 T
|
Components
|
Component Number
|
Component Name
|
C230106E
|
C230106S
|
|
C230106-A
|
4×qPCR Reaction Buffer
|
250 μL
|
1 mL
|
|
C230106-B
|
Primer & Probe Mix
|
25 μL
|
100 μL
|
|
C230106-C
|
Internal Control (IC)
|
25 μL
|
100 μL
|
|
C230106-D*
|
Positive Control (PC)
|
500 μL
|
2 mL
|
|
C230106-E**
|
DNA Dilution Buffer
|
1 mL
|
4×1 mL
|
|
C230106-F***
|
ddH2O
|
500 μL
|
2×1 mL
|
*PC：Positive control solution, with a concentration of 1, 000 copies/µL;
**DNA Dilution Buffer: Used for sample and IC dilution, as well as template for NTC and NC;
***ddH2O: Used for preparing qPCR Mix system.
Storage
Store at temperatures ranging from -15°C to -25°C with a shelf life of one year.
Please note that component C230106-B should be stored protected from light.
