qPCR/RT-qPCR

Product description The ROX Reference Dye is used on real-time PCR amplification instruments from companies such as ABI and Stratagene to correct inter-well errors caused by pipetting. The concentration of this product is 25 µM. Components Components No. N132049E N132049S N132049M Size 100 μL 500 μL 1 mL Shipping and Storage Dry ice shipping. Packaging and storage in the dark to avoid repeated freezing and thawing.-15℃ ~ -25℃ storage, valid for two years.For short-term use, it can be kept at 4°C. Notes 1. For your safety and health, please wear lab coats and disposable gloves for operation. 2. This product is for research use ONLY! Instructions 1. 50× ROX Reference Dye For the quantitative system preparation: Add 1 µL of 50× ROX Reference Dye to a 50 µL qPCR system. 2. 50× ROX Reference Dye II 1) Preparation method for 50× ROX Reference Dye II: Dilute the 50× ROX Reference Dye by 10 times*. ROX Dilution Solution: 20 mM Tris-HCl (pH 8.4), 0.1 mM EDTA, 0.01% Tween 20. 2) For the quantitative system preparation: Add 1 µL of 50× ROX Reference Dye II to a 50 µL qPCR system**. 【Note】: *It can be diluted and added proportionally according to the size of the system; **For multi-well systems, it is recommended to prepare a pre-mixed solution, and use it after thorough mixing. Applicable Models 1. 50× ROX Reference Dye applicable models ABI 5700, 7000, 7300, 7700, 7900HT Fast, StepOne, StepOne Plus. 2. 50× ROX Reference Dye II applicable models ABI 7500, 7500 Fast, ViiA7, QuantStudio 3 and 5, QuantStudio 6, 7, 12k Flex. Stratagene MX3000P, MX3005P, MX4000P 3. Instrument models that do not require Rox correction Bio-Rad CFX96, CFX384, iCycler iQ, iQ 5, MyiQ, MiniOpticon, Opticon, Opticon 2, Chromo4. Roche Applied Science LightCycler 480, LightCycler 2.0; LightCycler 96. Eppendorf Mastercycler ep realplex, realplex 2 s. Qiagen Corbett Rotor-Gene Q, Rotor-Gene 3000, Rotor-Gene 6000. Thermo Scientific PikoReal Cycler. Cepheid SmartCycler. Illumina Eco qPCR.

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