NGS Enzymes

phi29 DNA polymerase is derived from the Bacillus subtilis bacteriophage. After genetic engineering, it exhibits excellent strand displacement and processive synthesis capabilities, enabling the synthesis of DNA fragments up to 70 kb in length. Additionally, it possesses strong 3’→5’ exonuclease proofreading activity. Primer 3’ end modifications are recommended in the recommended reaction system to prevent degradation. This enzyme is commonly used in in vitro plasmid synthesis and whole genome amplification. Specifications Cat.No. N210508S / N210508M / N210508L Size 250 U / 1000 U / 5000 U Unit Definition The amount of enzyme required to incorporate 0.5 pmol of dNTP into acid-insoluble material in 10 minutes at 30°C. Heat Inactivation 65°C for 10 min. Components Components No. Name N210508S N210508M N210508L N210508-A phi29 DNA Polymerase (10 U/μL） 25 μL 100 μL 500 μL N210508-B 10×phi29 Reaction Buffer 100 μL 400 μL 2×1 mL Storage This product should be stored at -25~-15℃ for 2 years. Documents： Manual

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DNA Polymerase I synthesizes DNA in the 5′→3′ direction complementary to a template in the presence of a primer (DNA or RNA) and dNTPs as substrates. This enzyme has a molecular weight of approximately 109,000 and possesses both double-stranded specific 5′→3′ exonuclease activity and single-stranded specific 3′→5′ exonuclease activity. It can be used alongside DNase I for nick translation and with RNase H for second-strand cDNA synthesis. Specifications Cat.No. N210511S / N210511M / N210511L Size 500 U / 2500 U / 10000 U Components Components No. Name N210511S N210511M N210511L N210511-A DNA polymerase I (10 U/μL) 50 μL 250 μL 1 mL N210511-B 10 × DNA Polymerase I Reaction Buffer 1 mL 1 × 5 mL 4 × 5 mL Storage This product should be stored at -25~-15℃ for 2 years. Documents： Manual

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T4 Polynucleotide Kinase is a polynucleotide 5'-hydroxyl kinase capable of catalyzing the transfer of the γ-phosphate group from ATP to the 5'-hydroxyl termini of oligonucleotide chains (double-stranded or single-stranded DNA or RNA) and to 3'-monophosphate nucleotides. This reaction is reversible. It also has 3'-phosphatase activity, removing 3'-phosphate groups from oligonucleotides' 3'-phosphorylated ends, deoxy-3'-monophosphate nucleotides, and deoxy-3'-diphosphate nucleotides. In the presence of ADP, T4 PNK exhibits 5'-phosphatase activity, facilitating the exchange of terminal 5'-phosphate groups between 5'-phosphorylated oligo/polynucleotides and ATP. Suitable for constructing DNA libraries and end-labeling to make probes. Specifications Cat.No. N210510S / N210510M / N210510L Size 500 U / 2500 U / 10000 U Concentration 10 U/µL Source Recombinantly expressed in E. coli Enzyme Activity Definition The amount of enzyme required to catalyze the transfer of 1 nmol [γ-³²P] ATP in 30 minutes at 37°C. Components Components No. Name N210510S N210510M N210510L N210510-A T4 polynucleotide Kinase (10 U/μL) 50 μL 250 μL 1 mL N210510-B 10×T4 PNK Buffer 500 μL 1 mL 2×1 mL [Note]: The 10×T4 PNK Buffer does not contain ATP; Users need to add it themselves with a recommended final concentration of 1 mM or use T4 DNA ligase buffer. Storage This product should be stored at -25~-15℃ for 1 year. Documents： Manual

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T4 DNA Polymerase catalyzes the synthesis of DNA in a 5'→3' direction in the presence of a template and primer. It possesses 3'→5' exonuclease activity but lacks 5'→3' exonuclease activity. Specifications Cat.No. N210507E / N210507S / N210507M / N210507L Size 100 U / 500 U / 2000 U / 5000 U Components Components No. Name N210507E N210507S N210507M N210507L N210507-A T4 DNA polymerase (5 U/μL) 20 μL 100 μL 400 μL 1 mL N210507-B 10 × Blue Buffer 100 μL 250 μL 500 μL 1.25 mL Unit Definition One unit (U) of enzyme is defined as the amount required to catalyze the incorporation of 10 nmol of deoxyribonucleotides (dNTPs) into polynucleotides in 30 minutes at 37°C. Product Applications Blunting of DNA 5' or 3' overhangs; Synthesis of labeled DNA probes through displacement reactions; Second strand synthesis during site-directed mutagenesis; PCR product cloning without the need for ligation reactions. Storage Shipped with dry ice.This product should be stored at -25℃ ~-15℃ for 1 year. Notes 1. For your safety and health, wear lab coats and disposable gloves during operation. 2. This product is for research use only! Documents： Manual

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