Description
phi29 DNA polymerase is derived from the Bacillus subtilis bacteriophage. After genetic engineering, it exhibits excellent strand displacement and processive synthesis capabilities, enabling the synthesis of DNA fragments up to 70 kb in length. Additionally, it possesses strong 3’→5’ exonuclease proofreading activity. Primer 3’ end modifications are recommended in the recommended reaction system to prevent degradation. This enzyme is commonly used in in vitro plasmid synthesis and whole genome amplification.
Specifications
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Cat.No.
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N210508S / N210508M / N210508L
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Size
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250 U / 1000 U / 5000 U
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Unit Definition
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The amount of enzyme required to incorporate 0.5 pmol of dNTP into acid-insoluble material in 10 minutes at 30°C.
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Heat Inactivation
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65°C for 10 min.
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Components
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Components No.
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Name
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N210508S
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N210508M
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N210508L
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N210508-A
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phi29 DNA Polymerase (10 U/μL）
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25 μL
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100 μL
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500 μL
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N210508-B
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10×phi29 Reaction Buffer
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100 μL
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400 μL
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2×1 mL
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Storage
This product should be stored at -25~-15℃ for 2 years.
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