Biochemical Reagents

Biochemical Reagents

5 products

  • D-Luciferin, Potassium Salt_C331501

    D-Luciferin, Potassium Salt_C331501

    Product description D-Luciferin is a commonly used substrate for luciferase in the field of biotechnology, particularly in in vivo live imaging techniques. When fluorescein is in excess, the quantum number of light produced is equal to Luciferase concentrations were positively correlated (see figure below). Plasmids carrying luciferase encoding gene (Luc) were transfected into cells and introduced into study animals such as rats and mice. Subsequently, D-luciferin is injected, and changes in light intensity are detected using bioluminescence imaging (BLI) to monitor disease progression or therapeutic efficacy of drugs in real-time. Alternatively, the impact of ATP on this reaction system can be utilized to indicate changes in energy or vital signs based on variations in bioluminescence intensity. D-Luciferin is also commonly used in in vitro research, including luciferase and ATP level analysis; reporter gene analysis; high-throughput sequencing; and various contamination detections. Currently, there are three forms of the product: D-luciferin (free acid), D-luciferin salt (sodium salt and potassium salt). The main difference lies in their dissolution properties: the former has weaker water solubility and solubility in buffer systems, unless dissolved in weak bases such as low concentration NaOH and KOH solutions. It can be dissolved in methanol and DMSO; the latter can be easily dissolved in water or buffer solutions, making it convenient to use, with non-toxic solvents, especially suitable for in vivo experiments. After being prepared into solutions, these three forms of the product have no substantial differences in most applications. Specifications English synonym (S)-4,5-Dihydro-2-(6-hydroxy-2-benzothiazolyl)-4-thiazolecarboxylic acid potassium salt; D-Luciferin firefly, potassium salt CAS NO. 115144-35-9 Formula C11H7N2O3S2K Molecular weight 318.42 g/mol Appearance Light yellow powder Solubility Soluble in water(60 mg/mL) Components Components No. C331501E C331501S C331501M C331501L Size 100 mg 500 mg 1 g 5 g Storage Store at -20°C in a dry and dark place. Shelf life is 1 year. Documents: Manuals C331501-EN-Manual.pdf

    $150.00 - $2,770.00

  • D-Luciferin, Sodium Salt_C331502

    D-Luciferin, Sodium Salt_C331502

    Product description D-luciferin is a common substrate for Luciferase and is widely used throughout biotechnology, especially in vivo imaging technology. Its mechanism of action involves oxidation and luminescence of luciferin (substrate) in the presence of ATP and luciferase enzyme. When luciferin is present in excess, the number of light photons produced is directly proportional to the concentration of luciferase enzyme (as illustrated in the diagram below). Plasmids carrying luciferase encoding gene (Luc) were transfected into cells and introduced into study animals such as rats and mice In vivo, Subsequent injection of luciferin allows for real-time monitoring of changes in light intensity using bioluminescence imaging (BLI), enabling the real-time monitoring of disease progression or the therapeutic efficacy of drugs. Additionally, the impact of ATP on this reaction system can be utilized, with changes in bioluminescence intensity indicating energy levels or vital signs. D-luciferin is also commonly used in vitro for various research purposes, including analysis of luciferase activity and ATP levels, reporter gene assays, high-throughput sequencing, and various contamination detection assays.D-luciferin (free acid) has weak solubility in water and in buffered solutions unless dissolved in weak bases such as NaOH and KOH solutions. It is soluble in methanol (10 mg/mL) and DMSO (50 mg/mL). However, D-luciferin sodium salt and potassium salt forms can be easily and rapidly dissolved in water or buffered solutions, making them convenient to use. These solvents are non-toxic and particularly suitable for in vivo experiments. In most applications, there are no substantial differences among these three forms of D-luciferin once they are dissolved in solution. Specifications English synonym (S)-4,5-Dihydro-2-(6-hydroxy-2-benzothiazolyl)-4-thiazolecarboxylic acid sodium salt; D-Luciferin firefly, sodium salt monohydrate; CAS NO. 103404-75-7 Formula NaC11H7N2O3S2·H2O Molecular weight 320.32 g/mol Appearance Light yellow powder Solubility Solube in water(100 mg/mL) Purity (HPLC) ≥95% Components Components No. C331502E C331502S C331502M C331502L C331502T Size 100 mg 500 mg 1 g 5 g 10 g Storage Dry ice shipping. -20℃ storage, valid for one year. Documents: Manuals C331502-EN-Manual.pdf

    $205.00 - $2,145.00

  • Dextran Sulfate Sodium Salt, MW36000~50000_C331601

    Dextran Sulfate Sodium Salt, MW36000~50000_C331601

    Product description Dextran sulfate sodium(DSS) is a polyanionic derivative of dextran, formed by the esterification reaction of dextran and chlorosulfonic acid. It is commonly used in colitis modeling research. DSS has several characteristics:1) It is a polyanionic compound soluble in water, forming a colorless aqueous solution; 2) It exhibits high purity and good stability; 3) It is naturally degradable. Inflammatory bowel disease (IBD) is a chronic, relapsing gastrointestinal infection that increases the risk of intestinal tumors, mainly including ulcerative colitis (UC) and Crohn’s disease (CD). Since its first reported use in 1985 for inducing ulcerative colitis-like symptoms in mice, using dextran sulfate sodium (DSS), numerous studies have confirmed the similarity of the DSS colitis model to human ulcerative colitis.The histological features, clinical manifestations, the disease site and cytokine proliferation of DSS colitis model are very similar to human ulcerative colitis (UC). The modeling conditions and operation methods of this model are simple, cost-effective, reproducible, easy to master, and can be easily adapted and standardized. Depending on the experimental objectives, the DSS concentration and administration timing can be adjusted to establish acute, chronic, or alternate acute-chronic models. Specifications English Synonym Dextran Sulfate Sodium Salt, DSS; Dextran Sodium sulfate CAS NO. 9011-18-1 Formula (C6H7Na3O14S3) n Molecular Weight 36, 000 ~50, 000 Da Appearance White or off-white powder Solubility Soluble in water, slightly soluble in ethanol. Components Components No. C331601E C331601S C331601M C331601L Size 25 g 100 g 500 g 1 kg Storage The product is shipped and stored at room temperature, valid for 2 years. Documents: Manuals C331601-EN-Manual.pdf

    $435.00 - $8,705.00

  • Caerulein_C331603


    Product description Caerulein is a decapeptide and an agonist of cholecystokinin (CCK) receptor. As a cholecystokinin analog, it can stimulate gastric, bile duct and pancreatic secretion, acting on pancreatic alveolar cells, leading to the secretion of large amounts of digestive enzymes and pancreatic juice, leading to acute edematous pancreatitis. Caerulein can be used to study signal transduction pathways mediated by NF-κB upregulated proteins such as intercellular adhesion molecule (ICAM-1), inflammation-related factors such as NADPH oxidase and Janus kinase. Cerulein can also be used to test gallbladder function and prevent gallbladder pain, renal colic and intermittent claudication pain. It is generally considered an antagonist of endogenous orphins and can be used in conjunction with LPS to establish animal models of pancreatitis. Cerulein has been successfully used to establish acute pancreatitis (AP) models in rats, mice, dogs, Syrian hamsters and other animals. The modeling mechanism is as follows: 1.Upregulate the expression of intercellular adhesion molecule (ICAM-1) in pancreatic acinar cells by stimulating intracellular NF-KB. Surface ICAM-1 in turn promotes neutrophil adhesion to acinar cells thereby enhancing pancreatic inflammatory effects; 2.Induction of pancreatic inflammation by disrupting digestive enzyme secretion and causing cytoplasmic vacuolization and acinar cell death, leading to pancreatic edema; 3.Activate inflammation-promoting factors. Specifications English Synonym Caerulein Sulfated; Cerulein, Ceruletide; [Tyr(SO3H)4]Caerulein CAS NO. 17650-98-5 Formula C58H73N13O21S2 Molecular Weight 1352.4 Appearance White powder Purity ≥97% Solubility Soluble in DMSO, insoluble in water Sequence pGlu-Gln-Asp-Tyr(SO3H)-Thr-Gly-Trp-Met-Asp-Phe-NH2 Components Components No. C331603E Size 1 mg Storage Dry ice shipping. Powder: -85℃~-65℃, valid for 2 years; -15℃~-25℃, valid for 1 year.Solution should be stored at -85°C to -65°C with a shelf life of 6 months; or at -15°C to -25°C with a shelf life of 1 month. Keep refrigerated and dry, avoiding repeated freeze-thaw cycles. Documents: Manuals C331603-EN-Manual.pdf


  • Streptozocin_C331605


    Product description Streptozocin (STZ) is an antibiotic produced by Streptomyces Aureobasidium pullulans. It has anti-tumor effects and is often used to treat pancreatic cancer. At the same time, STZ can selectively destroy pancreatic beta cells in certain species of animals and induce diabetes. It can be used for modeling research on diabetes models. Rats and mice are generally used to create animal models. The preparation of type Ⅰ diabetes and type Ⅱ diabetes animal models is related to the dose of STZ injection: when a high dose is injected, it can directly cause extensive destruction of pancreatic islet β cells, which can cause a type Ⅰ diabetes model; when a smaller amount of STZ is injected, it only destroys the function of a part of the pancreatic islet β cells, causing peripheral tissues to be insensitive to insulin. At the same time, high-calorie feed is given. The combination of the two can induce an animal model with pathological and physiological changes close to human type Ⅱ diabetes. Generally speaking, high doses of STZ can induce type I diabetes models, while low doses of STZ plus high-fat and high-sugar feed can induce type II diabetes models. In addition, STZ has also been widely studied in anti-leukemia, DNA methylation, anti-nephritis and other aspects. Specifications English Synonym Streptozotocin CAS NO. 18883-66-4 Formula C8H15N3O7 Molecular Weight 265.22 Appearance White or light yellow powder Purity ≥98% Solubility Soluble in water, lower alcohols, ketones, etc. Structure Components Components No. C331605E C331605S C331605M Size 100 mg 500 mg 1 g Storage Ice pack shipping. -15℃ ~-25℃ storage, away from moisture and light, valid for 2 years. Documents: Manuals C331605-EN-Manual.pdf

    $65.00 - $285.00

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