D-Luciferin, Potassium Salt_C331501

Description

Product description

D-Luciferin is a commonly used substrate for luciferase in the field of biotechnology, particularly in in vivo live imaging techniques. When fluorescein is in excess, the quantum number of light produced is equal to Luciferase concentrations were positively correlated (see figure below). Plasmids carrying luciferase encoding gene (Luc) were transfected into cells and introduced into study animals such as rats and mice. Subsequently, D-luciferin is injected, and changes in light intensity are detected using bioluminescence imaging (BLI) to monitor disease progression or therapeutic efficacy of drugs in real-time. Alternatively, the impact of ATP on this reaction system can be utilized to indicate changes in energy or vital signs based on variations in bioluminescence intensity.


D-Luciferin is also commonly used in in vitro research, including luciferase and ATP level analysis; reporter gene analysis; high-throughput sequencing; and various contamination detections. Currently, there are three forms of the product: D-luciferin (free acid), D-luciferin salt (sodium salt and potassium salt). The main difference lies in their dissolution properties: the former has weaker water solubility and solubility in buffer systems, unless dissolved in weak bases such as low concentration NaOH and KOH solutions. It can be dissolved in methanol and DMSO; the latter can be easily dissolved in water or buffer solutions, making it convenient to use, with non-toxic solvents, especially suitable for in vivo experiments. After being prepared into solutions, these three forms of the product have no substantial differences in most applications.


Specifications

English synonym

(S)-4,5-Dihydro-2-(6-hydroxy-2-benzothiazolyl)-4-thiazolecarboxylic acid potassium salt; D-Luciferin firefly, potassium salt

CAS NO.

115144-35-9

Formula

C11H7N2O3S2K

Molecular weight

318.42 g/mol

Appearance

Light yellow powder

Solubility

Soluble in water(60 mg/mL)


Components

Components No.

C331501E

C331501S

C331501M

C331501L

Size

100 mg

500 mg

1 g

5 g


Storage

Store at -20°C in a dry and dark place. Shelf life is 1 year.

Documents:

D-Luciferin, Potassium Salt_C331501

Product form

SKU: C331501E

$150.00

    • Tell a unique detail about this product

    Description

    Product description

    D-Luciferin is a commonly used substrate for luciferase in the field of biotechnology, particularly in in vivo live imaging techniques. When fluorescein is in excess, the quantum number of light produced is equal to Luciferase concentrations were positively correlated (see figure below). Plasmids carrying luciferase encoding gene (Luc) were transfected into cells and introduced into study animals such as rats and mice. Subsequently, D-luciferin is injected, and changes in light intensity are detected using bioluminescence imaging (BLI) to monitor disease progression or therapeutic efficacy of drugs in real-time. Alternatively, the impact of ATP on this reaction system can be utilized to indicate changes in energy or vital signs based on variations in bioluminescence intensity.


    D-Luciferin is also commonly used in in vitro research, including luciferase and ATP level analysis; reporter gene analysis; high-throughput sequencing; and various contamination detections. Currently, there are three forms of the product: D-luciferin (free acid), D-luciferin salt (sodium salt and potassium salt). The main difference lies in their dissolution properties: the former has weaker water solubility and solubility in buffer systems, unless dissolved in weak bases such as low concentration NaOH and KOH solutions. It can be dissolved in methanol and DMSO; the latter can be easily dissolved in water or buffer solutions, making it convenient to use, with non-toxic solvents, especially suitable for in vivo experiments. After being prepared into solutions, these three forms of the product have no substantial differences in most applications.


    Specifications

    English synonym

    (S)-4,5-Dihydro-2-(6-hydroxy-2-benzothiazolyl)-4-thiazolecarboxylic acid potassium salt; D-Luciferin firefly, potassium salt

    CAS NO.

    115144-35-9

    Formula

    C11H7N2O3S2K

    Molecular weight

    318.42 g/mol

    Appearance

    Light yellow powder

    Solubility

    Soluble in water(60 mg/mL)


    Components

    Components No.

    C331501E

    C331501S

    C331501M

    C331501L

    Size

    100 mg

    500 mg

    1 g

    5 g


    Storage

    Store at -20°C in a dry and dark place. Shelf life is 1 year.

    Documents:

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