D-Luciferin, Sodium Salt_C331502

Description

Product description

D-luciferin is a common substrate for Luciferase and is widely used throughout biotechnology, especially in vivo imaging technology. Its mechanism of action involves oxidation and luminescence of luciferin (substrate) in the presence of ATP and luciferase enzyme. When luciferin is present in excess, the number of light photons produced is directly proportional to the concentration of luciferase enzyme (as illustrated in the diagram below). Plasmids carrying luciferase encoding gene (Luc) were transfected into cells and introduced into study animals such as rats and mice In vivo, Subsequent injection of luciferin allows for real-time monitoring of changes in light intensity using bioluminescence imaging (BLI), enabling the real-time monitoring of disease progression or the therapeutic efficacy of drugs. Additionally, the impact of ATP on this reaction system can be utilized, with changes in bioluminescence intensity indicating energy levels or vital signs.

D-luciferin is also commonly used in vitro for various research purposes, including analysis of luciferase activity and ATP levels, reporter gene assays, high-throughput sequencing, and various contamination detection assays.D-luciferin (free acid) has weak solubility in water and in buffered solutions unless dissolved in weak bases such as NaOH and KOH solutions. It is soluble in methanol (10 mg/mL) and DMSO (50 mg/mL). However, D-luciferin sodium salt and potassium salt forms can be easily and rapidly dissolved in water or buffered solutions, making them convenient to use. These solvents are non-toxic and particularly suitable for in vivo experiments. In most applications, there are no substantial differences among these three forms of D-luciferin once they are dissolved in solution.


Specifications

English synonym

(S)-4,5-Dihydro-2-(6-hydroxy-2-benzothiazolyl)-4-thiazolecarboxylic acid sodium salt; D-Luciferin firefly, sodium salt monohydrate;

CAS NO.

103404-75-7

Formula

NaC11H7N2O3S2·H2O

Molecular weight

320.32 g/mol

Appearance

Light yellow powder

Solubility

Solube in water(100 mg/mL)

Purity (HPLC)

≥95%


Components

Components No.

C331502E

C331502S

C331502M

C331502L

C331502T

Size

100 mg

500 mg

1 g

5 g

10 g


Storage

Dry ice shipping. -20℃ storage, valid for one year.

Documents:

D-Luciferin, Sodium Salt_C331502

Product form

SKU: C331502E

$205.00

    • Tell a unique detail about this product

    Description

    Product description

    D-luciferin is a common substrate for Luciferase and is widely used throughout biotechnology, especially in vivo imaging technology. Its mechanism of action involves oxidation and luminescence of luciferin (substrate) in the presence of ATP and luciferase enzyme. When luciferin is present in excess, the number of light photons produced is directly proportional to the concentration of luciferase enzyme (as illustrated in the diagram below). Plasmids carrying luciferase encoding gene (Luc) were transfected into cells and introduced into study animals such as rats and mice In vivo, Subsequent injection of luciferin allows for real-time monitoring of changes in light intensity using bioluminescence imaging (BLI), enabling the real-time monitoring of disease progression or the therapeutic efficacy of drugs. Additionally, the impact of ATP on this reaction system can be utilized, with changes in bioluminescence intensity indicating energy levels or vital signs.

    D-luciferin is also commonly used in vitro for various research purposes, including analysis of luciferase activity and ATP levels, reporter gene assays, high-throughput sequencing, and various contamination detection assays.D-luciferin (free acid) has weak solubility in water and in buffered solutions unless dissolved in weak bases such as NaOH and KOH solutions. It is soluble in methanol (10 mg/mL) and DMSO (50 mg/mL). However, D-luciferin sodium salt and potassium salt forms can be easily and rapidly dissolved in water or buffered solutions, making them convenient to use. These solvents are non-toxic and particularly suitable for in vivo experiments. In most applications, there are no substantial differences among these three forms of D-luciferin once they are dissolved in solution.


    Specifications

    English synonym

    (S)-4,5-Dihydro-2-(6-hydroxy-2-benzothiazolyl)-4-thiazolecarboxylic acid sodium salt; D-Luciferin firefly, sodium salt monohydrate;

    CAS NO.

    103404-75-7

    Formula

    NaC11H7N2O3S2·H2O

    Molecular weight

    320.32 g/mol

    Appearance

    Light yellow powder

    Solubility

    Solube in water(100 mg/mL)

    Purity (HPLC)

    ≥95%


    Components

    Components No.

    C331502E

    C331502S

    C331502M

    C331502L

    C331502T

    Size

    100 mg

    500 mg

    1 g

    5 g

    10 g


    Storage

    Dry ice shipping. -20℃ storage, valid for one year.

    Documents:

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