Description
T4 Polynucleotide Kinase is a polynucleotide 5'-hydroxyl kinase capable of catalyzing the transfer of the γ-phosphate group from ATP to the 5'-hydroxyl termini of oligonucleotide chains (double-stranded or single-stranded DNA or RNA) and to 3'-monophosphate nucleotides. This reaction is reversible. It also has 3'-phosphatase activity, removing 3'-phosphate groups from oligonucleotides' 3'-phosphorylated ends, deoxy-3'-monophosphate nucleotides, and deoxy-3'-diphosphate nucleotides. In the presence of ADP, T4 PNK exhibits 5'-phosphatase activity, facilitating the exchange of terminal 5'-phosphate groups between 5'-phosphorylated oligo/polynucleotides and ATP. Suitable for constructing DNA libraries and end-labeling to make probes.
Specifications
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Cat.No.
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N210510S / N210510M / N210510L
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Size
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500 U / 2500 U / 10000 U
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Concentration
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10 U/µL
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Source
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Recombinantly expressed in E. coli
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Enzyme Activity Definition
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The amount of enzyme required to catalyze the transfer of 1 nmol [γ-³²P] ATP in 30 minutes at 37°C.
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Components
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Components No.
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Name
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N210510S
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N210510M
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N210510L
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N210510-A
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T4 polynucleotide Kinase (10 U/μL)
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50 μL
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250 μL
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1 mL
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N210510-B
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10×T4 PNK Buffer
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500 μL
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1 mL
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2×1 mL
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[Note]: The 10×T4 PNK Buffer does not contain ATP; Users need to add it themselves with a recommended final concentration of 1 mM or use T4 DNA ligase buffer.
Storage
This product should be stored at -25~-15℃ for 1 year.
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