Description
VEGF 165, also known as Vascular Endothelial Growth Factor 165, is a protein that plays a critical role in angiogenesis, the process of forming new blood vessels from pre-existing ones. It belongs to the vascular endothelial growth factor family and is one of the most well-studied isoforms of VEGF.
Product Properties
Synonyms
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VEGF-165;VEGF; VEGFA; MVCD1; VPF; RP1-261G23.1;
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Uniprot No.
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P15692-4
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Source
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Recombinant Human VEGF165 Protein is expressed from HEK293 without tag. It contains Ala27-Arg191 |
Molecular Weight
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The protein has a predicted MW of 19.2 kDa. Due to glycosylation, the protein migrates to 20-30 kDa based on Tris-Bis PAGE result.
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Biological Activity
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ED50 < 3 ng/ml, measured in a cell proliferation assay using HUVEC cells.
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Purity
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> 95% by SDS-PAGE and HPLC
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Endotoxin
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<1.0 EU per 1μg of the protein by the LAL method.
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Formulation
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Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
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Reconstitution
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It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
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Shipping and Storage
The product should be stored at -25~-15℃ for 1 year from date of receipt.
2-7 days, 2 ~8 °C under sterile conditions after reconstitution.
3 -6 months, -85~-65℃ under sterile conditions after reconstitution.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
Cautions
1.Please operate with lab coats and disposable gloves,for your safety.
2.This product is for research use only.