Recombinant Human Wnt -3a Protein_C230259

Description

Wnt3a, one of Wnt family members, plays key roles in regulating pleiotropic cellular functions, including self-renewal, proliferation, differentiation, and motility. The Wnt family comprises 19 human proteins, including Wnt1, Wnt2, Wnt2b (Wnt13), Wnt3, Wnt3a, Wnt4, Wnt5a, Wnt5b, Wnt6, Wnt7a, Wnt7b, Wnt8a, Wnt8b, Wnt9a (Wnt14), Wnt9b (Wnt14b), Wnt10a, Wnt10b, Wnt11, and Wnt16. These genes encode secreted glycoproteins that are rich in cysteine. Wnts can combine with cell membrane receptors that play a critical role in autocrine regulation and/or participate in paracrine modification by binding to adjacent cell membrane receptors. The signal transduction pathway mediated by Wnt genes is called the Wnt signaling pathway. Accumulating evidence has suggested that Wnt3a promotes or suppresses tumor progression via the canonical Wnt signaling pathway depending on cancer type. In addition, the roles of Wnt3a signaling can be inhibited by multiple proteins or chemicals. Human Wnt-3a shares 96% aa identity with mouse mouse, bovine and canine Wnt-3a, and 89%, 86% and 84% aa identity with chicken, Xenopus and zebrafish Wnt-3a, respectively. It also shares 87% aa identity with Wnt3. During embryonic development, Wnt-3a is necessary for proper development of the hippocampus, anterior-posterior patterning, somite development, and tailbud formation. Wnt-3a also promotes self-renewal of  hematopoietic stem cells, neural stem cells, and embryonic stem cells.

Product Properties

Synonyms

Wingless-type MMTV Integration Site Family, Member 3
Uniprot No.
P56704
GeneID
8978
Source
HEK293 cells-derived Human wnt-3a, Ser19-Lys352
Molecular Weight
Approximately 63.3 kDa
AA Sequence
SY PIWWSLAVGP QYSSLGSQPI LCASIPGLVP KQLRFCRNYV EIMPSVAEGI KIGIQECQHQ 
FRGRRWNCTT VHDSLAIFGP VLDKATRESA FVHAIASAGV AFAVTRSCAE GTAAICGCSS RHQGSPGKGW KWGGCSEDIE FGGMVSREFA
DARENRPDAR SAMNRHNNEA GRQAIASHMH LKCKCHGLSG
SCEVKTCWWS QPDFRAIGDF LKDKYDSASE MVVEKHRESR
GWVETLRPRY TYFKVPTERD LVYYEASPNF CEPNPETGSF GTRDRTCNVS
SHGIDGCDLL CCGRGHNARA ERRREKCRCV FHWCCYVSCQ ECTRVYDVHT CK
Tag
Fc
Purity
> 75% as determined by SDS-PAGE.
Activity
Measured by its binding ability in a ELISA method. Immobilized Human Wnt-3a at 10 μg/ml (100 μl/well) can bind bind Wnt-3a mouse monoclonal antibody.
Endotoxin
< 1.0 EU per 1μg of the protein by the LAL method.
Formulation
Lyophilized from a 0.2 µm filtered concentrated solution in PBS.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions.


Transportation and Storage Methods

Transport with ice packs. Store at -20°C to -80°C with a one-year shelf life. It is recommended to aliquot and freeze for the first use to avoid repeated freeze-thaw cycles.
Cautions

1.Avoid repeated freezing and thawing.

2.For your safety and health, please wear a lab coat and use disposable gloves during operations.

3.This product is for research use only!

Recombinant Human Wnt -3a Protein_C230259

Product form

SKU: C230259E

$4,073.00

      • Tell a unique detail about this product

      Description

      Wnt3a, one of Wnt family members, plays key roles in regulating pleiotropic cellular functions, including self-renewal, proliferation, differentiation, and motility. The Wnt family comprises 19 human proteins, including Wnt1, Wnt2, Wnt2b (Wnt13), Wnt3, Wnt3a, Wnt4, Wnt5a, Wnt5b, Wnt6, Wnt7a, Wnt7b, Wnt8a, Wnt8b, Wnt9a (Wnt14), Wnt9b (Wnt14b), Wnt10a, Wnt10b, Wnt11, and Wnt16. These genes encode secreted glycoproteins that are rich in cysteine. Wnts can combine with cell membrane receptors that play a critical role in autocrine regulation and/or participate in paracrine modification by binding to adjacent cell membrane receptors. The signal transduction pathway mediated by Wnt genes is called the Wnt signaling pathway. Accumulating evidence has suggested that Wnt3a promotes or suppresses tumor progression via the canonical Wnt signaling pathway depending on cancer type. In addition, the roles of Wnt3a signaling can be inhibited by multiple proteins or chemicals. Human Wnt-3a shares 96% aa identity with mouse mouse, bovine and canine Wnt-3a, and 89%, 86% and 84% aa identity with chicken, Xenopus and zebrafish Wnt-3a, respectively. It also shares 87% aa identity with Wnt3. During embryonic development, Wnt-3a is necessary for proper development of the hippocampus, anterior-posterior patterning, somite development, and tailbud formation. Wnt-3a also promotes self-renewal of  hematopoietic stem cells, neural stem cells, and embryonic stem cells.

      Product Properties

      Synonyms

      Wingless-type MMTV Integration Site Family, Member 3
      Uniprot No.
      P56704
      GeneID
      8978
      Source
      HEK293 cells-derived Human wnt-3a, Ser19-Lys352
      Molecular Weight
      Approximately 63.3 kDa
      AA Sequence
      SY PIWWSLAVGP QYSSLGSQPI LCASIPGLVP KQLRFCRNYV EIMPSVAEGI KIGIQECQHQ 
      FRGRRWNCTT VHDSLAIFGP VLDKATRESA FVHAIASAGV AFAVTRSCAE GTAAICGCSS RHQGSPGKGW KWGGCSEDIE FGGMVSREFA
      DARENRPDAR SAMNRHNNEA GRQAIASHMH LKCKCHGLSG
      SCEVKTCWWS QPDFRAIGDF LKDKYDSASE MVVEKHRESR
      GWVETLRPRY TYFKVPTERD LVYYEASPNF CEPNPETGSF GTRDRTCNVS
      SHGIDGCDLL CCGRGHNARA ERRREKCRCV FHWCCYVSCQ ECTRVYDVHT CK
      Tag
      Fc
      Purity
      > 75% as determined by SDS-PAGE.
      Activity
      Measured by its binding ability in a ELISA method. Immobilized Human Wnt-3a at 10 μg/ml (100 μl/well) can bind bind Wnt-3a mouse monoclonal antibody.
      Endotoxin
      < 1.0 EU per 1μg of the protein by the LAL method.
      Formulation
      Lyophilized from a 0.2 µm filtered concentrated solution in PBS.
      Reconstitution
      We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions.


      Transportation and Storage Methods

      Transport with ice packs. Store at -20°C to -80°C with a one-year shelf life. It is recommended to aliquot and freeze for the first use to avoid repeated freeze-thaw cycles.
      Cautions

      1.Avoid repeated freezing and thawing.

      2.For your safety and health, please wear a lab coat and use disposable gloves during operations.

      3.This product is for research use only!

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