Description
Activin A is a member of the TGF-β family and possesses a wide range of biological activities, including the regulation of cell proliferation and differentiation, as well as the promotion of neuronal survival. Elevated levels of Activin A in human colorectal cancer and postmenopausal women are associated with colorectal cancer and breast cancer, respectively. The biological activity of Activin A can be neutralized by inhibins and diffusible TGF-β antagonists such as Follistatin. Activin A binds to two forms of Activin type I receptors (ActRI-A and ActRI-B) and two forms of Activin type II receptors (ActRII-A and ActRII-B). Activins are homo- or heterodimers of different β subunits. They are produced as precursor proteins with an N-terminal propeptide that is cleaved to release the C-terminal bioactive ligand.
The product is provided in the form of lyophilized powder, derived from HEK293 cells, with high activity and high purity. Recombinant human/mouse/rat Activin A is a 26.0kDa homodimer of two βA chains linked by disulfide bonds, each containing 116 amino acid residues.
Product Properties
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Source
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Human Activin A Protein is expressed from HEK293 without tag.
It contains Gly311-Ser426.
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Accession
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P08467
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Molecular Weight
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The protein has a predicted MW of 12.97 kDa. Due to glycosylation, the protein migrates to 14-15 kDa under reduced (R) condition, and 23-25 kDa under Non reducing (N) condition based on Tris-Bis PAGE result. |
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Endotoxin
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Less than 0.1EU per μg by the LAL method.
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Purity
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> 95% as determined by Tris-Bis PAGE
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Activity
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Measured by its ability to inhibit proliferation of MPC-11 cells. The ED50 for this effect is < 7 ng/mL.
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Formulation
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Lyophilized from 0.22μm filtered solution in 4mM HCI. Normally 8% trehalose is added as protectant before lyophilization.
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Shipping and Storage
Store at -25 to -15°C, and the shelf life is 1 year after receipt of the goods.
After reconstitution, store at 2 to 8°C, and the shelf life is 7 days. After reconstitution, store at -85 to -65°C, and the shelf life is 3 months.
It is recommended to divide the protein equally upon first use to avoid repeated freeze-thaw cycles.
Reconstitution conditions
Before opening the vial, centrifuge it first. Reconstitute with 4 mM HCl to a concentration no lower than 0.1 mg/mL.
Cautions
1. For your safety and health, please wear a lab coat and use disposable gloves when handling.
2.This product is intended for research purposes only.
