Recombinant Human TGF-beta 3 Protein, His Tag_C230258

Description

TGFβ3 is a member of the TGF-β superfamily subgroup, defined by its structural and functional similarities. TGF-β3, along with β1 and β2, acts as a cellular switch to regulate immune function, cell proliferation, and epithelial-mesenchymal transition. The human TGF-β3 cDNA encodes a 412 amino acid (aa) precursor, which contains a 20 aa signal peptide and a 392 aa protein. Mature human TGF-β3 shares 100%, 99%, and 98% homology with mouse/dog/horse, rat, and pig TGF-β3, respectively. TGF-β3 has a high affinity for TGF-βRII, a type II serine/threonine kinase receptor. This receptor phosphorylates and activates the type I serine/threonine kinase receptor TGF-βRI or ALK-1 to regulate transcription through Smad phosphorylation.

The product is provided in the form of a lyophilized (freeze-dried) powder, characterized by high activity, high purity, and low endotoxin levels.


Product Properties

Synonyms
TGF-β3; ARVD; ARVD1; FLJ16571; LDS5; RNHF; TGFB3; TGFbeta 3; TGF-beta 3; TGF-beta3; TGF-beta-3; transforming growth factor beta-3; transforming growth factor beta 3
Uniprot No.
P10600
Expression System and Range
CHO-derived Human TGF-β3, Ala 301-Ser 412
Molecular Weight
Approximately 13 kDa
AA Sequence
MALDTNYCFRN LEENCCVRPL YIDFRQDLGW KWVHEPKGYY ANFCSGPCPY LRSADTTHST VLGLYNTLNP EASASPCCVP QDLEPLTILY YVGRTPKVEQ LSNMVVKSCK CS
Appearance
White powder, Colorless clear liquid after reconstitution
Purity
≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining.
Biological Activity
Measured in a cell inhibition assay using TF-1 cells at IL4 presence. The ED50 for this effect is ≤0.2ng/mL.
Endotoxin
< 10 EU/mg of the protein by the LAL method
Formulation
Lyophilized from a 0.22 μm-filtered solution containing 100 mM Glycine, 150 mM NaCl, 5% mannitol and 0.01% Tween 80, pH 4.0

 

Method of Use

It is recommended to centrifuge before opening the cap to bring the contents to the bottom, and then resuspend with sterile deionized water.

Transportation and Storage Methods

Store at -20°C, with a one-year shelf life upon receipt. After resuspension, store at 2~8°C for a shelf life of 7-10 days. After resuspension, store at -85 to -65°C for a shelf life of six months. It is recommended to aliquot and freeze for the first use to avoid repeated freeze-thaw cycles.

Cautions

1.For your safety and health, please wear a lab coat and use disposable gloves when operating.

2.This product is intended for scientific research purposes only.

Recombinant Human TGF-beta 3 Protein, His Tag_C230258

Product form

SKU: C230258E

$74.00

    • Shipped today? Order within: Jan 17, 2025 17:00:00 -0500
    • Tell a unique detail about this product

    Description

    TGFβ3 is a member of the TGF-β superfamily subgroup, defined by its structural and functional similarities. TGF-β3, along with β1 and β2, acts as a cellular switch to regulate immune function, cell proliferation, and epithelial-mesenchymal transition. The human TGF-β3 cDNA encodes a 412 amino acid (aa) precursor, which contains a 20 aa signal peptide and a 392 aa protein. Mature human TGF-β3 shares 100%, 99%, and 98% homology with mouse/dog/horse, rat, and pig TGF-β3, respectively. TGF-β3 has a high affinity for TGF-βRII, a type II serine/threonine kinase receptor. This receptor phosphorylates and activates the type I serine/threonine kinase receptor TGF-βRI or ALK-1 to regulate transcription through Smad phosphorylation.

    The product is provided in the form of a lyophilized (freeze-dried) powder, characterized by high activity, high purity, and low endotoxin levels.


    Product Properties

    Synonyms
    TGF-β3; ARVD; ARVD1; FLJ16571; LDS5; RNHF; TGFB3; TGFbeta 3; TGF-beta 3; TGF-beta3; TGF-beta-3; transforming growth factor beta-3; transforming growth factor beta 3
    Uniprot No.
    P10600
    Expression System and Range
    CHO-derived Human TGF-β3, Ala 301-Ser 412
    Molecular Weight
    Approximately 13 kDa
    AA Sequence
    MALDTNYCFRN LEENCCVRPL YIDFRQDLGW KWVHEPKGYY ANFCSGPCPY LRSADTTHST VLGLYNTLNP EASASPCCVP QDLEPLTILY YVGRTPKVEQ LSNMVVKSCK CS
    Appearance
    White powder, Colorless clear liquid after reconstitution
    Purity
    ≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining.
    Biological Activity
    Measured in a cell inhibition assay using TF-1 cells at IL4 presence. The ED50 for this effect is ≤0.2ng/mL.
    Endotoxin
    < 10 EU/mg of the protein by the LAL method
    Formulation
    Lyophilized from a 0.22 μm-filtered solution containing 100 mM Glycine, 150 mM NaCl, 5% mannitol and 0.01% Tween 80, pH 4.0

     

    Method of Use

    It is recommended to centrifuge before opening the cap to bring the contents to the bottom, and then resuspend with sterile deionized water.

    Transportation and Storage Methods

    Store at -20°C, with a one-year shelf life upon receipt. After resuspension, store at 2~8°C for a shelf life of 7-10 days. After resuspension, store at -85 to -65°C for a shelf life of six months. It is recommended to aliquot and freeze for the first use to avoid repeated freeze-thaw cycles.

    Cautions

    1.For your safety and health, please wear a lab coat and use disposable gloves when operating.

    2.This product is intended for scientific research purposes only.

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