IL-6 is similar to other interleukins in that it has a compact globular folding structure and is held in place by two disulfide bonds. The cysteine involved in forming these two disulfide bonds is highly conserved. IL-6, expressed by a variety of cells, regulates cell growth and differentiation in various tissues and is an important pro-inflammatory and immunomodulatory cytokine.
Product Properties
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Synonyms
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Interleukin-6,BSF2,HSF,IFNB2
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Uniprot No.
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P05231
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Source
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Recombinant Human IL-6 Protein is expressed from HEK293 Cells with His tag at the C-terminal. It contains Val30-Met212.
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Molecular Weight
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The protein has a predicted MW of 22.75 kDa. And it migrates as 25-30 kDa under reducing
(R) condition (SDS-PAGE) due to glycosylation.
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Purity
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> 95% as determined by SDS-PAGE.
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Biological Activity
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The ED50 as determined by a cell proliferation assay using human TF-1 cells is 0.23-0.48 ng/mL, corresponding to a specific activity of > 2.0 × 106 IU/mg.
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Endotoxin
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< 1 EU per 1μg of the protein by the LAL method.
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Formulation
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Lyophilized from a 0.2 μm filtered concentrated solution in PBS.
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Reconstitution
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Centrifuge tubes before opening. Dissolve lyophilized protein with PBS. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
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Shipping and Storage
The product should be stored at -25~-15℃ for 1 year from date of receipt. 2-7 days, 2 ~8 °C under sterile conditions after reconstitution.
3 months, -25~-15℃ under sterile conditions after reconstitution.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
Cautions
1.Please operate with lab coats and disposable gloves,for your safety.
2.This product is for research use only.
