Description
Transforming Growth Factor-alpha (TGF-α) , also known as sarcoma growth factor, TGF-type I and ETGF, is a member of the EGF family of cytokines. TGF-α is an EGF-related polypeptide growth factor that signals through the EGF receptor, and stimulates the
proliferation of a wide range of epidermal and epithelial cells.Mature TGF-alpha shows approximately 93% amino acid sequence identity with mouse or rat TGF-alpha and is not species specific in its biological effects.
Product Properties
Synonyms
|
Transforming Growth Factor alpha; TGF-a; TGF-type I; ETGF
|
Accession
|
|
|
7039
|
Source
|
E.coli-derived Human TGF-a protein, Vla40-Ala89
|
|
Approximately 5.8 kDa, on SDS-PAGE under reducing conditions
|
AA Sequence
|
V VSHFNDCPDS HTQFCFHGTC RFLVQEDKPA CVCHSGYVGA RCEHADLLA
|
Tag
|
|
Physical Appearance
|
Sterile Filtered White lyophilized (freeze-dried) powder
|
|
> 95% as determined by SDS-PAG
|
|
Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. The ED50 for this effect is < 0.4 ng/mL.
|
|
< 1.0 EU per 1μg of the protein by the LAL method
|
|
Lyophilized after extensive dialysis against PBS.
|
|
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O or PBS up to 100 μg/ml.For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles.
|
Shipping and Storage
The products are shipped with ice pack and can be stored at -80℃ for 1 year from date of receipt.
1week, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 °C under sterile conditions after reconstitution.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
Cautions
1. For your safety and health, please wear lab coats and disposable gloves for operation.
2. For research use only!