Description
Interleukin-18 (IL-18) is a potent pro-inflammatory cytokine that can induce the production of interferon-gamma in Th1 cells, NK cells, and activated macrophages, especially in the presence of IL-12. IL-18 also plays a role in developmental regulation. This product features high activity, high purity, and low endotoxin levels. The product is verified and tested for biological activity, endotoxin levels, and through SDS-PAGE to ensure the biological activity and batch-to-batch consistency; our product is provided in a carrier-free form, making it highly suitable for research and production in cell culture, ELISA, or as a standard for immunoblotting.
Product Properties
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Aliases
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Interleukin 18; Interleukin-18
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Uniprot No.
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P70380
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Expression Range and System
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E.coli-derived Mouse IL-18 protein, Asn36-Ser192 with a His tag at the C-terminus.
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Molecular Weight
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Approximately 18 kDa.
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Appearance
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Sterile Filtered White lyophilized (freeze-dried) powder.
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Purity
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Greater than 98% as determined by SDS-PAGE.
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Activity
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Measured by its ability to induce IFN gamma secretion in KG-1 cells. The ED50 for this effect is 0.5 μg/mL.
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Endotoxin
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Less than 0.1 EU per 1μg of the protein by the LAL method.
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Formulation
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Lyophilized (freeze-dried) from a solution containing 1×PBS, pH 8.0.
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Usage Instructions
1.It is recommended to reconstitute the lyophilized powder with sterile water to a concentration of no less than 100 μg/mL and let it stand for at least 20 minutes to ensure complete dissolution.
2.After reconstitution, the solution can be further diluted and aliquoted for storage at 2-8°C with a shelf life of 1 month, or at -20°C with a shelf life of 3 months; avoid repeated freeze-thaw cycles.
3.When further diluting and aliquoting the reconstituted solution, it is necessary to add a certain amount of carrier protein (0.1% BSA, 10% FBS, or 5% HSA). For serum-free experimental requirements, it can be replaced with a 5% trehalose solution as the carrier.
Shipping and Storage
Ice Pack Shipping:Store at -20°C, with a one-year shelf life.
Recommendation for Initial Use: It is advised to aliquot and freeze the product for storage upon the first use to prevent repeated freezing and thawing.
Cautions
1. For your safety and health, please wear a lab coat and use disposable gloves when handling.
2.This product is for research use only.
