Recombinant Mouse IL-18 Protein, His Tag_C230251

Description

Interleukin-18 (IL-18) is a potent pro-inflammatory cytokine that can induce the production of interferon-gamma in Th1 cells, NK cells, and activated macrophages, especially in the presence of IL-12. IL-18 also plays a role in developmental regulation. This product features high activity, high purity, and low endotoxin levels. The product is verified and tested for biological activity, endotoxin levels, and through SDS-PAGE to ensure the biological activity and batch-to-batch consistency; our product is provided in a carrier-free form, making it highly suitable for research and production in cell culture, ELISA, or as a standard for immunoblotting.

Product Properties

Aliases

Interleukin 18; Interleukin-18

Uniprot No.

P70380

Expression Range and System

E.coli-derived Mouse IL-18 protein, Asn36-Ser192 with a His tag at the C-terminus.

Molecular Weight

Approximately 18 kDa.

Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Purity

Greater than 98% as determined by SDS-PAGE.

Activity

Measured by its ability to induce IFN gamma secretion in KG-1 cells. The ED50 for this effect is 0.5 μg/mL.

Endotoxin

Less than 0.1 EU per 1μg of the protein by the LAL method.

Formulation

Lyophilized (freeze-dried) from a solution containing 1×PBS, pH 8.0.

 

Usage Instructions

1.It is recommended to reconstitute the lyophilized powder with sterile water to a concentration of no less than 100 μg/mL and let it stand for at least 20 minutes to ensure complete dissolution.

2.After reconstitution, the solution can be further diluted and aliquoted for storage at 2-8°C with a shelf life of 1 month, or at -20°C with a shelf life of 3 months; avoid repeated freeze-thaw cycles.

3.When further diluting and aliquoting the reconstituted solution, it is necessary to add a certain amount of carrier protein (0.1% BSA, 10% FBS, or 5% HSA). For serum-free experimental requirements, it can be replaced with a 5% trehalose solution as the carrier.

 

Shipping and Storage

Ice Pack Shipping:Store at -20°C, with a one-year shelf life.

Recommendation for Initial Use: It is advised to aliquot and freeze the product for storage upon the first use to prevent repeated freezing and thawing.

 

Cautions

1. For your safety and health, please wear a lab coat and use disposable gloves when handling.

2.This product is for research use only.

Recombinant Mouse IL-18 Protein, His Tag_C230251

Product form

SKU: C230251E

$74.00

    • Shipped today? Order within: Jul 17, 2024 17:00:00 -0400
    • Tell a unique detail about this product

    Description

    Interleukin-18 (IL-18) is a potent pro-inflammatory cytokine that can induce the production of interferon-gamma in Th1 cells, NK cells, and activated macrophages, especially in the presence of IL-12. IL-18 also plays a role in developmental regulation. This product features high activity, high purity, and low endotoxin levels. The product is verified and tested for biological activity, endotoxin levels, and through SDS-PAGE to ensure the biological activity and batch-to-batch consistency; our product is provided in a carrier-free form, making it highly suitable for research and production in cell culture, ELISA, or as a standard for immunoblotting.

    Product Properties

    Aliases

    Interleukin 18; Interleukin-18

    Uniprot No.

    P70380

    Expression Range and System

    E.coli-derived Mouse IL-18 protein, Asn36-Ser192 with a His tag at the C-terminus.

    Molecular Weight

    Approximately 18 kDa.

    Appearance

    Sterile Filtered White lyophilized (freeze-dried) powder.

    Purity

    Greater than 98% as determined by SDS-PAGE.

    Activity

    Measured by its ability to induce IFN gamma secretion in KG-1 cells. The ED50 for this effect is 0.5 μg/mL.

    Endotoxin

    Less than 0.1 EU per 1μg of the protein by the LAL method.

    Formulation

    Lyophilized (freeze-dried) from a solution containing 1×PBS, pH 8.0.

     

    Usage Instructions

    1.It is recommended to reconstitute the lyophilized powder with sterile water to a concentration of no less than 100 μg/mL and let it stand for at least 20 minutes to ensure complete dissolution.

    2.After reconstitution, the solution can be further diluted and aliquoted for storage at 2-8°C with a shelf life of 1 month, or at -20°C with a shelf life of 3 months; avoid repeated freeze-thaw cycles.

    3.When further diluting and aliquoting the reconstituted solution, it is necessary to add a certain amount of carrier protein (0.1% BSA, 10% FBS, or 5% HSA). For serum-free experimental requirements, it can be replaced with a 5% trehalose solution as the carrier.

     

    Shipping and Storage

    Ice Pack Shipping:Store at -20°C, with a one-year shelf life.

    Recommendation for Initial Use: It is advised to aliquot and freeze the product for storage upon the first use to prevent repeated freezing and thawing.

     

    Cautions

    1. For your safety and health, please wear a lab coat and use disposable gloves when handling.

    2.This product is for research use only.

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