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Mouse IL-12p70 ELISA (Enzyme-Linked Immunosorbent Assay) Kit is an in vitro enzyme-linked immunosorbent assay kit used for quantitative determination of IL-12p70 in serum, plasma, and cell culture supernatants. Specific anti-IL-12p70 antibodies are pre-coated onto high-affinity enzyme plates. Standards and samples are added to the wells of the enzyme plate, and after incubation, IL-12p70 present in the sample binds to the solid-phase antibody. After washing to remove unbound substances, a detection antibody is added for binding and incubated, followed by washing, and then the enzyme complex (Streptavidin-HRP) is added for binding and incubated. After washing, a colorimetric substrateTMB is added for color development, avoiding light. The intensity of the color reaction is proportional to the concentration of IL-12p70 in the sample. The reaction is terminated by adding a stop solution, and the absorbance is measured at 450 nm wavelength (with a reference wavelength of 570 - 630 nm). Interleukin-12 (IL-12) is a heterodimeric pro-inflammatory cytokine best known for inducing IFN-γ production in T cells and NK cells. IL-12 consists of two disulfide-linked subunits (p35 and p40) that regulate the biosynthesis of IL-12p70. The effects of IL-12 are mediated through its high-affinity receptor that contains two subunits: IL-12Rβ1 and IL-12Rβ2. IL-12 is produced by macrophages and B cells and has been shown to have multiple effects on T cells and natural killer (NK) cells. While mouse IL-12 is active on both human and mouse cells, human IL-12 is not active on mouse cells. Specification Item Number P162016S / P162016E Specification 48 T / 96 T Detection Range 31.25-2000 pg/mL Detection Method Sandwich ELISA Detection Species Mouse Detection Time 4.5 hours Sensitivity 7.19 pg/mL Dilution Linearity 96 - 124% Recovery Rate 82 - 122% Intra-assay Variability 3.2% Components Component Number Component Name Storage Temperature P162016S P162016E P162016-A Plate 2~8℃ 48 T 96 T P162016-B Standard 2~8℃ 1 tube 2 tubes P162016-C Detection Antibody 2~8℃ 120 μL 240 μL P162016-D Enzyme Conjugate 2~8℃(Avoid Light) 30 μL 60 μL P162016-E 5× Dilution Buffer 2~8℃ 8 mL 15 mL P162016-F 20× Wash Buffer 2~8℃ 15 mL 30 mL P162016-G Substrate Solution 2~8℃(Avoid Light) 8 mL 15 mL P162016-H Stop Solution Room Temperature 5 mL 10 mL P162016-I Plate Sealers Room Temperature 3 pieces 5 pieces Storage The assay kit can be stored at 2~8℃. Alternatively, the reagents can be stored according to the storage conditions provided in the component information to avoid contamination and repeated freeze-thaw cycles. Diluted working solutions should be used immediately and not reused. The shelf life is 1 year. Table 1 Reagent Storage Table After First Use  Material Name Storage Conditions Plate   Unused strips can be returned to the aluminum foil bag, tightly sealed, and stored at 2~8°C to avoid moisture absorption. Standard Use within 48 hours after dissolution, store at 2~8°C to avoid contamination. Detection Antibody Use within 48 hours after dilution, store at 2~8°C to avoid contamination. Enzyme conjugate Use within 48 hours after dilution, store at 2~8°C to avoid contamination. 5×Dilution Buffer Store at 2~8°C for 1 month, avoiding contamination. 20×Wash Buffer Store at 2~8°C for 1 month, avoiding contamination. Substrate solution Store at 2~8°C for 1 month, avoiding light exposure. Stop Solution Can be stored at room temperature. Plate Sealers Can be stored at room temperature.                                    Documents: Manuals P162016-EN-Manual.pdf

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Mouse interleukin 1β (Mouse IL-1β/IL-1F2), as a major member of the IL-1 family, is primarily produced by activated macrophages. It stimulates thymocyte proliferation through induction of IL-2 release, promotes B-cell maturation and proliferation, and activates fibroblast growth factor to stimulate thymocyte proliferation. It can also stimulate synovial cells to release prostaglandins and various collagenases. IL-1β plays a central role in physiological phenomena such as immunity and inflammation, bone remodeling, fever, and carbohydrate metabolism. Aberrant IL-1β signaling drives tumorigenesis by promoting epithelial-to-mesenchymal transition, increasing production of inflammatory cytokines and chemokines, inducing immune suppression and resistance to cell apoptosis, and increasing leukocyte adhesion. IL-1β-induced inflammation-related diseases include diabetes, arthritis, and atherosclerosis. Moreover, abnormal activation of IL-1β is also associated with poor prognosis in most cancer types, including colon cancer, lung cancer, and breast cancer, among others. The Arcegen Mouse IL-1β/IL-1F2 ELISA (Enzyme-Linked Immunosorbent Assay) Kit is an in vitro enzyme-linked immunosorbent assay kit used for quantitative determination of Mouse interleukin 1β (Mouse IL-1β/IL-1F2) in serum and plasma. Specific antibodies against Mouse interleukin 1β are pre-coated on a high-affinity ELISA plate. Standard samples and test samples are added to the wells of the ELISA plate, and after incubation, Mouse interleukin 1β present in the samples binds to the solid-phase antibody. After washing to remove unbound substances, a detection antibody is added for incubation and binding, followed by washing and addition of enzyme conjugate (Streptavidin-HRP) for incubation and binding. After washing, a colorimetric substrate TMB is added for color development under light-shielding conditions. The intensity of the color reaction is directly proportional to the concentration of Mouse interleukin 1β in the samples. The reaction is terminated by adding stop solution, and the absorbance is measured at 450 nm wavelength (reference wavelength 570~630 nm). Specification Catalog Number P162004S / P162004E Specification 48 T / 96 T Detection Range 15.63~1000 pg/mL Detection Method Sandwich ELISA Detection Time 4.5 hours Sensitivity 11.46 pg/mL Dilution Linearity 89~123% Recovery Rate 78~107% Intra-assay Variability 4.0% Inter-assay Variability 3.9% Components Component Number Component Name Storage Temperature P162004S P162004E P162004-A ELISA Plate 2~8℃ 48 T 96 T P162004-B Standard 2~8℃ 1 tube 2 tubes P162004-C Detection Antibody 2~8℃ 120 μL 240 μL P162004-D Enzyme Conjugate 2~8℃(Avoid Light) 30 μL 60 μL P162004-E Sample Dilution Buffer 2~8℃ 8 mL 15 mL P162004-F Antibody/Enzyme Dilution Buffer 2~8℃ 15 mL 30 mL P162004-G 20x Wash Buffer 2~8℃ 25 mL 50 mL P162004-H Substrate Solution 2~8℃(Avoid Light) 8 mL 15 mL P162004-I Stop Solution Room Temperature 5 mL 10 mL P162004-J Plate Sealant Film Room Temperature 3 pieces 5 pieces Shipping and Storage Reagent Kit can be stored at 2~8°C or according to the storage conditions provided for each component to prevent contamination and repeated freeze-thaw cycles. Dilute reagents to working concentrations immediately before use and discard them afterward; they should not be reused. The shelf life is 1 year. Table 1 Reagent Storage Table After Initial Use  Material Name Storage Conditions Enzyme-linked immunosorbent assay (ELISA)    Unused strips can be returned to the aluminum foil bag, tightly sealed, and stored at 2~8°C to avoid moisture absorption. plate Standard sample Use within 48 hours after dissolution, store at 2~8°C to avoid contamination. Detecting antibody Use within 48 hours after dilution, store at 2~8°C to avoid contamination. Enzyme conjugate Use within 48 hours after dilution, store at 2~8°C to avoid contamination. Sample diluent Store at 2~8°C for 1 month, avoiding contamination. 20×Wash solution Store at 2~8°C for 1 month, avoiding contamination. Antibody/enzyme diluent Store at 2~8°C for 1 month, avoiding light exposure. Substrate solution Store at 2~8°C for 1 month, avoiding light exposure. Stop solution Can be stored at room temperature. Plate seal film Can be stored at room temperature.                                    Documents: Manuals P162004-EN-Manual.pdf

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Mouse IL-20 ELISA (Enzyme-Linked Immunosorbent Assay) Kit is an in vitro enzyme-linked immunosorbent assay kit used for quantitative determination of IL-20 in serum, plasma, and cell culture supernatants. Specific anti-IL-20 antibodies are pre-coated onto high-affinity enzyme plates. Standards and samples are added to the wells of the enzyme plate, and after incubation, IL-20 present in the sample binds to the solid-phase antibody. After washing to remove unbound substances, a detection antibody is added for binding and incubated, followed by washing, and then the enzyme complex (Streptavidin-HRP) is added for binding and incubated. After washing, a colorimetric substrateTMB is added for color development, avoiding light. The intensity of the color reaction is proportional to the concentration of IL-20 in the sample. The reaction is terminated by adding a stop solution, and the absorbance is measured at 450 nm wavelength (with a reference wavelength of 570 - 630 nm). IL-20 belongs to the TGF-β superfamily. IL-20 binds two heterodimeric receptor complexes, IL-20 R alpha/IL-20 R beta and IL-22 R/IL-20 R beta. IL-20 is reported to enhances tissue remodeling and wound-healing activities and restores the homeostasis of epithelial layers during infection and inflammatory responses to maintain tissue integrity. Specification Item Number P162017S / P162017E Specification 48 T / 96 T Detection Range 31.25-2000 pg/mL Detection Method Sandwich ELISA Detection Species Mouse Detection Time 4.5 hours Sensitivity 8.22 pg/mL Dilution Linearity 91 - 120% Recovery Rate 91 - 115% Intra-assay Variability 3.7% Intra-assay Variability 6.1% Components Component Number Component Name Storage Temperature P162017S P162017E P162017-A ELISA Plate 2~8℃ 48 T 96 T P162017-B Standard 2~8℃ 1 tube 2 tubes P162017-C Detection Antibody 2~8℃ 60 μL 120 μL P162017-D Enzyme Conjugate 2~8℃(Avoid Light) 30 μL 60 μL P162017-E 5x Dilution Buffer 2~8℃ 8 mL 15 mL P162017-F 20x Wash Buffer 2~8℃ 25 mL 50 mL P162017-G Substrate Solution 2~8℃(Avoid Light) 8 mL 15 mL P162017-H Stop Solution Room Temperature 5 mL 10 mL P162017-I Plate Sealers Room Temperature 3 pieces 5 pieces Storage The assay kit can be stored at 2~8℃. Alternatively, the reagents can be stored according to the storage conditions provided in the component information to avoid contamination and repeated freeze-thaw cycles. Diluted working solutions should be used immediately and not reused. The shelf life is 1 year. Table 1 Reagent Storage Table After First Use  Material Name Storage Conditions Plate   Unused strips can be returned to the aluminum foil bag, tightly sealed, and stored at 2~8°C to avoid moisture absorption. Standard Use within 48 hours after dissolution, store at 2~8°C to avoid contamination. Detection Antibody Use within 48 hours after dilution, store at 2~8°C to avoid contamination. Enzyme conjugate Use within 48 hours after dilution, store at 2~8°C to avoid contamination. 5×Dilution Buffer Store at 2~8°C for 1 month, avoiding contamination. 20×Wash Buffer Store at 2~8°C for 1 month, avoiding contamination. Substrate solution Store at 2~8°C for 1 month, avoiding light exposure. Stop Solution Can be stored at room temperature. Plate Sealers Can be stored at room temperature.                                    Documents: Manuals P162017-EN-Manual.pdf

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Interleukin-6 (IL-6) was initially identified as a B cell differentiation factor and is now known to be a multifunctional cytokine that regulates immune responses, hematopoiesis, acute-phase reactions, and inflammation. It has three receptor binding sites, including one specific receptor IL-6R binding site, and two gp130 binding sites. IL-6 is a pleiotropic, alpha-helical, 22~28 kDa glycoprotein that plays important roles in acute-phase reactions, inflammation, hematopoiesis, bone metabolism, and cancer progression. Human IL-6 shares 39% amino acid sequence identity with mouse and rat IL-6. It is a multifunctional cytokine that not only affects the immune system but also acts on various biological systems and physiological events in various organs, while also inducing the growth of bone marrow and plasma cell tumors. The Arcegen Mouse IL-6 ELISA (Enzyme-Linked Immunosorbent Assay) Kit is an in vitro enzyme-linked immunosorbent assay kit used for the quantitative determination of mouse interleukin-6 (Mouse IL-6) in serum and plasma. Specific antibodies against mouse interleukin-6 are pre-coated on a high-affinity enzyme immunoassay plate. Standard samples and test samples are added to the wells of the plate, and after incubation, the mouse interleukin-6 present in the samples binds to the solid-phase antibody. After washing to remove unbound substances, a detection antibody is added and incubated for binding. After washing, enzyme conjugate (Streptavidin-HRP) is added and incubated for binding. After washing, a color substrate TMB is added for color development in the dark. The intensity of the color reaction is proportional to the concentration of mouse interleukin-6 in the sample. The reaction is terminated by adding a stop solution, and the absorbance is measured at 450 nm wavelength (with a reference wavelength of 570~630 nm). Specification Catalog Number P162003S/P162003E Specification 48 T / 96 T Detection Range 15.63~1000 pg/mL Detection Method ELISA Detection Time 4.5 hours Sensitivity 9.37 pg/mL Dilution Linearity 74~107% Recovery Rate 77~111% Intra-assay Variability 3.1% Inter-assay Variability 5.7% Components Component Number Component Name Storage Temperature P162003S P162003E P162003-A ELISA Plate 2~8℃ 48 T 96 T P162003-B Standard 2~8℃ 1 tube 2 tubes P162003-C Detection Antibody 2~8℃ 120 μL 240 μL P162003-D Enzyme Conjugate 2~8℃(Avoid Light) 30 μL 60 μL P162003-E 5×Dilution Buffer 2~8℃ 8 mL 15 mL P162003-F 20×Wash Buffer 2~8℃ 25 mL 50 mL P162003-G Substrate Solution 2~8℃(Avoid Light) 8 mL 15 mL P162003-H Stop Solution Room Temperature 5 mL 10 mL P162003-I Plate Sealant Film Room Temperature 3 pieces 5 pieces Shipping and Storage Reagent Kit can be stored at 2~8°C or according to the storage conditions provided for each component to prevent contamination and repeated freeze-thaw cycles. Dilute reagents to working concentrations immediately before use and discard them afterward; they should not be reused. The shelf life is 1 year. Table 1 Reagent Storage Table After Initial Use  Material Name Storage Conditions Enzyme Plate  Unused strips can be returned to aluminum foil pouch, tightly sealed, and stored at 2~8°C to avoid moisture absorption. Standard Use within 48 hours after dissolution, store at 2~8°C to avoid contamination. Detection Antibody Use within 48 hours after dilution, store at 2~8°C to avoid contamination. Enzyme Conjugate Use within 48 hours after dilution, store at 2~8°C to avoid contamination. 5×Dilution Buffer Store at 2~8°C for 1 month, avoid contamination 20×Wash Solution Store at 2~8°C for 1 month, avoid contamination Substrate Solution Store at 2~8°C for 1 month, protect from light. Stop Solution Can be stored at room temperature Sealing Film Can be stored at room temperature                                    Documents: Manuals P162003-EN-Manual.pdf

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Product description The kit is equipped with a powerful lysis buffer, which can quickly lyse samples (e.g. mouse tail, mouse ear, mouse toe, muscle, etc.) to release genomic DNA, and the lysate can be directly added to the PCR reaction system without purification, which is convenient for operation. In addition, this kit requires low sample input, 5 mg of mouse tissue or 1-5 mm of mouse tail can be used for experiments. Components Components No. Name N132028E (50 T) N132028S  (200 T) N132028-A Buffer ML 5×1 mL 20 mL N132028-B Buffer MT 0.6 mL 2×1.25 mL 1) Buffer ML is a lysis buffer containing strong protein denaturants, please wear gloves. 2) Buffer MT is a stop buffer used to stop the lysis function of Buffer ML. Storage 1. Component A: The product should be stored at 2-8℃ for one year. For multiple use for a long time, please avoid cross-contamination. 2. Component B: The product should be stored at -25 ~ -15℃ for one year. Please avoid repeated freeze-thaw. Notes 1. This product is for research use only. 2. Please operate with lab coats and disposable gloves，for your safety. 3. In order to avoid cross contamination between samples, it is necessary to immerse the edge of the sampling device or the part in direct contact with the sample in 2% sodium hypochlorite solution after each sampling, wash it several times, and then dry the residual liquid with a clean paper towel before using it again. For the convenience of the test, several sampling devices can be prepared and cleaned uniformly after use to ensure that each individual sample is sampled with a contamination-free sampling device. 4. It is recommended to use freshly taken animal tissues. In case of long-term frozen tissues, repeated freezing and thawing should be avoided as much as possible, otherwise it will lead to degradation of the template and affect the PCR efficiency. Instructions Mouse Genomic DNA Release 1. Clip 5-10 mg of animal tissue or 1-5 mm mouse tail in a 1.5 mL centrifuge tube*. 【Note】: * Tissue should be cut up as much as possible to allow the lysis reaction to proceed more smoothly. 2. Add 90 μL of Buffer ML to the above centrifuge tube, vortex gently to completely infiltrate the sample with the lysate, and centrifuge briefly. 3. Incubate at 95°C for 15 min in a thermostatic incubator**. 【Note】: **Incubation at 95°C for 15 min is generally sufficient for most PCR needs. If a larger amount of DNA is required or the sample is difficult to lyse, the time can be extended to 30 min. The tissue block does not need to be completely lysed, and the residue can be removed in a subsequent centrifugation step. 4. Add 10 μL of Buffer MT, flick to mix, and terminate lysis. 5. Optional step: centrifugation at 12000 rpm for 2 min. 6. Transfer the supernatant to a new centrifuge tube and store at 4°C or -20°C or take the supernatant directly for subsequent PCR amplification. Cell Genomic DNA Release After culturing transfected cells in a 48-well-plate for 3 days and reaching a cell density of about 70,000 cells/well, remove the medium as completely as possible. Then add 90 μL of ML buffer directly per well and pipette each well. Transfer all to the 96 well-PCR plate. After treating with a PCR machine at 95 °C for 5-15 min and cooling down, add 10 μL MT buffer and blow evenly and thoroughly. Using high-fidelity enzyme (Cat# N132016) or Taq enzyme, add 0.5-2 μL cell lysate to every 50 uL of PCR reaction system and perform PCR.

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Tumor necrosis factor-alpha (TNF-α), also known as cachexin or TNFSF1A, is a multifunctional cytokine that plays a central role in inflammation, apoptosis, and immune system development. It is produced by various immune cells, epithelial cells, endothelial cells, and tumor cells. TNF-α exerts its effects through receptors TNFR1 and TNFR2, activating signaling pathways involving caspase 8, transcription factor NF-κB, and kinase JNK. Human TNF-α shares 79% amino acid homology with mouse TNF-α, suggesting that its biological functions may not exhibit significant species specificity. TNF-α has been shown to confer resistance against certain types of infections while paradoxically inducing inflammation in pathological processes. It may also affect glucose uptake and insulin resistance. Additionally, TNF-α plays a critical role in tumor proliferation, migration, invasion, and angiogenesis. The Arcegen Mouse TNF-α ELISA assay kit is an in vitro enzyme-linked immunosorbent assay (ELISA) kit designed to quantitatively measure mouse TNF-α in serum and plasma samples. The assay utilizes high-affinity anti-mouse TNF-α antibodies pre-coated onto an ELISA plate. Standard samples and test samples are added to the plate wells and incubated, allowing mouse TNF-α present in the samples to bind to the solid-phase antibodies. After washing to remove unbound substances, a detection antibody is added for incubation, followed by addition of enzyme conjugate (Streptavidin-HRP). After washing again, a colorimetric substrate (TMB) is added for color development, and the intensity of the color reaction is proportional to the concentration of mouse TNF-α in the samples. The reaction is terminated, and absorbance is measured at 450 nm wavelength (with a reference wavelength of 570-630 nm). Specification Item Number P162006S / P162006E Specification 48 T / 96 T Detection Range 31.25-2000 pg/mL Detection Method Sandwich ELISA Species Detected Mouse Detection Time 4.5 hours Sensitivity 7.82 pg/mL Dilution Linearity 84 - 124% Recovery Rate 81 - 119% Intra-assay Variability 3.8% Intra-assay Variability 5.3% Components Component Number Component Name Storage Temperature P162006S P162006E P162006-A ELISA Plate 2~8℃ 48 T 96 T P162006-B Standard sample 2~8℃ 1 tube 2 tubes P162006-C Detection antibody 2~8℃ 120 μL 240 μL P162006-D Enzyme conjugate 2~8℃(Avoid Light) 30 μL 60 μL P162006-E 5× dilution buffer 2~8℃ 8 mL 15 mL P162006-F 20× wash buffer 2~8℃ 25 mL 50 mL P162006-G Substrate solution 2~8℃(Avoid Light) 8 mL 15 mL P162006-H Stop solution Room Temperature 5 mL 10 mL P162006-I Plate sealing film Room Temperature 3 pieces 5 pieces Storage The kit can be stored at 2~8°C, or according to the storage conditions of individual components to avoid contamination and repeated freeze-thaw cycles. Diluted reagents prepared to working concentration should be used immediately and discarded; they should not be reused. The shelf life is 1 year. Table 1 Reagent Storage Table After Initial Use  Component Name Storage Conditions ELISA Plate   Unused strips can be returned to the aluminum foil bag, tightly sealed, and stored at 2~8°C to avoid moisture absorption. Standard sample Use within 48 hours after dissolution, store at 2~8°C to avoid contamination. Detection antibody Use within 48 hours after dilution, store at 2~8°C to avoid contamination. Enzyme conjugate Use within 48 hours after dilution, store at 2~8°C to avoid contamination. 5× dilution buffer Store at 2~8°C for 1 month, avoiding contamination. 20× wash buffer Store at 2~8°C for 1 month, avoiding contamination. Substrate solution Store at 2~8°C for 1 month, avoiding light exposure. Stop solution Can be stored at room temperature. Plate sealing film Can be stored at room temperature.                                    Documents: Manuals P162006-EN-Manual.pdf

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Vascular Endothelial Growth Factor (VEGF) is the most specific regulator of angiogenesis, providing a morphological basis for endothelial cell migration and tumor cell metastasis. As a foundation for tumor stroma and capillary network formation, VEGF can inhibit apoptosis of tumor cells, although the mechanism remains unclear. The Arcegen Mouse VEGF ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay kit used for quantitative measurement of Mouse VEGF in mouse serum, plasma, and cell culture supernatants. High-affinity anti-VEGF antibodies are precoated on the enzyme-linked immunosorbent assay plate. Standards and test samples are added to the plate wells, and after incubation, VEGF present in the samples binds to the solid-phase antibodies. After washing to remove unbound substances, detection antibodies (biotin-labeled) are added and incubated to bind. After washing, enzyme conjugate (HRP-labeled streptavidin) is added and incubated to bind. Following washing, a colorimetric substrate, TMB, is added for color development in the absence of light. The intensity of the color reaction is directly proportional to the concentration of VEGF in the sample. The reaction is terminated by adding stop solution, and absorbance values are measured at 450 nm as the primary wavelength and 630 nm as the secondary wavelength. Specification Catalog Number P162005S / P162005E Specification 48 T / 96 T Detection Range 6.25~400 pg/mL Detection Method Sandwich ELISA Species Detected Mouse Detection Time 4 hours 30 minutes Sensitivity 0.11 pg/mL Dilution Linearity 96.7~126.9% Recovery Rate 77.1~124% Intra-assay Variability 4.4% Intra-assay Variability 6.33% Components Component Number Component Name Storage Temperature P162005S P162005E P162005-A ELISA Plate 2~8℃ 48 T 96 T P162005-B Standard 2~8℃ 1 tube 2 tubes P162005-C Detection Antibody 2~8℃ 120 μL 240 μL P162005-D Enzyme Conjugate 2~8℃(Avoid Light) 30 μL 60 μL P162005-E 5×Dilution Buffer 1 2~8℃ 12 mL 25 mL P162005-F 5×Dilution Buffer 2 2~8℃ 8 mL 15 mL P162005-G 20×Wash Buffer 2~8℃ 25 mL 50 mL P162005-H Substrate Solution 2~8℃(Avoid Light) 8 mL 15 mL P162005-I Stop Solution Room Temperature 5 mL 10 mL P162005-J Plate Sealing Film Room Temperature 3 pieces 5 pieces Storage The kit can be stored at 2~8°C or according to the storage conditions provided for each component to avoid contamination and repeated freeze-thaw cycles. Diluted working concentration reagents should be used immediately and discarded; they should not be reused. The shelf life is 1 year. Table 1 Reagent Storage Table After Initial Use Component Name Storage Conditions ELISA Plate   Unused strips can be returned to the aluminum foil bag, tightly sealed, and stored at 2~8°C to avoid moisture absorption. Standard Use within 48 hours after dissolution, store at 2~8°C to avoid contamination. Detection Antibody Use within 48 hours after dilution, store at 2~8°C to avoid contamination. Enzyme Conjugate Use within 48 hours after dilution, store at 2~8°C to avoid contamination. 5×Dilution Buffer 1 Store at 2~8°C for 1 month, avoiding contamination. 5×Dilution Buffer 2 Store at 2~8°C for 1 month, avoiding contamination. 20×Wash Buffer Store at 2~8°C for 1 month, avoiding contamination. Substrate Solution Store at 2~8°C for 1 month, avoiding light exposure. Stop solution Can be stored at room temperature. Plate Sealing Film Can be stored at room temperature.                                    Documents: Manuals P162005-EN-Manual.pdf

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Product description This kit can directly and quickly conduct PCR amplification of mouse tissue (such as mouse tail, mouse ear, mouse toe, muscle, etc.), and has strong sample compatibility. Equipped with a powerful lysis buffer, this kit can rapidly lyse samples and release genomic DNA. The lysate can be directly added to the PCR reaction system without purification, and the operation is convenient. In addition, this kit requires low sample input, and 5 mg mouse tissue or 1-5 mm mouse tail can be used for experiments. The 2×Mouse Direct PCR Mix provided by this kit is a hot-start PCR reaction solution with a 2-fold concentration. It contains all the components used for PCR amplification except the template and primers, which greatly simplifies the operation process and reduces the chance of contamination. The kit can be used for transgene identification, mouse genotyping, etc. Components Components No. Name N132021E (50 T) N132021M (200 T) N132021-A Buffer ML 5×1 mL 20×1 mL N132021-B Buffer MT 0.6 mL 2×1.25 mL N132021-C 2×Mouse Direct PCR Mix 500 μL 2×1 mL 【Note】: 1) Buffer ML is a lysis buffer containing strong protein denaturants, please wear gloves. 2) Buffer MT is a stop buffer used to stop the lysis function of Buffer ML. 3) 2×Mouse Direct PCR Mix: Contains hot-start Taq DNA polymerase, dNTP mix, MgCl2, reaction buffer, PCR reaction enhancer, optimizer, stabilizer, electrophoresis indicator dye, etc. Specifications Product specification Kit Hot Start Built-in Hot Start Overhang Blunt Conditions for transportation Ice Packs Product Type Direct PCR Kit Apply to (application) Mouse tail, Mouse ear, Rat toe, Viscera, Skin, etc. Storage 1. Component A: The product should be stored at 2-8℃ for one year. For multiple use for a long time, please avoid cross-contamination. 2. Component B/C: The product should be stored at -25 ~ -15℃ for one year. Please avoid repeated freeze-thaw. Applications This product is primarily applied to the genotyping of mice. Notes 1. To avoid sample to sample cross contamination, the edge of the sampling equipment or the site in direct  contact with the sample was immersed in 2% sodium hypochlorite solution after each sampling session,  washed repeatedly several times, and then blotted with clean paper towels to dry the residual liquid before use. For the convenience of the test, multiple sampling equipment can also be prepared and cleaned uniformly after use to ensure that each individual sample uses a non-polluting sampling equipment. 2. It is recommended to use freshly collected animal tissue. If it is a long-term frozen tissue, repeated freezing and thawing should be avoided as much as possible, otherwise the template will be degraded and the PCR efficiency will be affected. 3. It is recommended to amplify the fragment length within 1 kb for the best amplification efficiency. 4. For your safety and health, please wear lab coats and disposable gloves for operation. 5. This product is for research use ONLY! Instructions 1. Sample genomic DNA release 1.1 Cut 5-10 mg of animal tissue or 1-5 mm of rat tail and put them in a 1.5 mL centrifuge tube. 1.2 Add 90 μL of Buffer ML to the above centrifuge tube, vortex gently so that the sample is completely infiltrated by the lysate, and centrifuge briefly. 1.3 Incubate at 95℃ for 15 min in a constant temperature incubator. 1.4 Add 10 μL Buffer MT, flick and mix to stop lysis. 1.5 Optional steps: centrifugation at 12,000 rpm for 2 min. 1.6 Transfer the supernatant to a new centrifuge tube and store at 4℃ or -20℃ or directly take the supernatant for subsequent PCR amplification. 【Note】: *The tissue should be chopped as little as possible to allow the lysis reaction to proceed more smoothly. **Incubate at 95℃, usually 15 min to meet most PCR needs. If a larger amount of DNA is required or the sample is difficult to lyse, the time can be extended to 30 min. The tissue mass does not need to be completely lysed. The residual fraction can be removed in a subsequent centrifugation step. 2. Reaction System Table 1  Reaction system Components Volume (μL) Final concentration 2×Mouse Direct PCR Mix 10 1× Forward Primer (10 μmol/L) 0.5 0.2-0.4 μmol/L Reverse Primer (10 μmol/L) 0.5 0.2-0.4 μmol/L Lysate product (DNA template) 1 - ddH2O to 20 - *All components should be thoroughly mixed before use. 2.1 Template usage: It is recommended to use 1-10% of the total system amount of template, and 1 μL supernatant is recommended as template for 20 μL system. 2.2 Primer final concentration: it is recommended to use 0.2-0.4 μmol/L of primer final concentration to get better results. When the reaction performance is poor, the primer concentration can be adjusted in the range of 0.1-0.5 μmol/L. 2.3 Reaction system: 20 μL is recommended, and the volume of the system can also be adjusted according to usage habits. 2.4 System preparation: Prepare the PCR reaction system, place it on a vortexer, vortex and mix, and centrifuge briefly to collect the reaction solution at the bottom of the tube. 3. Reaction Program Table 2  Reaction Program Cycle steps Temperature (°C) Time Cycles Predenaturation 94 5 min 1 Denaturation 94 10 sec 35 Annealing 60 20 sec Extension 72 30 sec/kb Final extension 72 5 min 1 3.1 Annealing temperature: please refer to the theoretical Tm value of the primer. The annealing temperature can be set 2-5℃ lower than the theoretical value of the primer. 3.2 Extension time: please set as 30 sec/kb. 3.3 Number of amplification cycles: 35 cycles can amplify enough products. 3.4 Sample loading by electrophoresis: take 3-5 μL of amplification product and load it. 4.  Control response In the analysis of PCR results, whether positive or negative, the reliability of the results cannot be determined without a control reaction. In order to facilitate the analysis of subsequent experimental results, it is recommended to set positive and negative PCR control reactions during PCR in order to exclude false positive or false negative interference.

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Product description This product is a closed-tube fluorescent quantitative RT-qPCR system based on RNA templates for simultaneous detection of multiple targets. It integrates the reverse transcription and quantitative amplification processes, significantly simplifying operational steps and maximizing control of cross-contamination risks. The kit features two core enzyme technologies: a high-performance thermostable reverse transcriptase for efficient first-strand cDNA synthesis, and a high-performance Taq DNA polymerase modified for hot-start to ensure precise quantitative amplification. The premixed system includes a specially optimized multiplex reaction buffer system that ensures independent amplification efficiency for each primer pair while enabling parallel detection of multiple targets. This product is designed for research needs such as multiplex pathogen detection, gene expression profiling, and simultaneous quantitative analysis of multiple indicators in complex samples. Specifications Catalog Number N132052E N132052S N132052M Specifications 50 T 100 T 1000 T Components Component Identification Component Name N132041E N132041S N132041M N132052-A 2×RT-qPCR Buffer 750 μL 1.5 mL 15 mL N132052-B 2×RT-qPCR Enzyme Mix 60 μL 120 μL 1.2 mL 【Notes】 1) The Buffer mainly contains dNTPs, dUTP, Mg², stabilizers, etc. 2) The Enzyme Mix mainly contains a high-performance thermostable reverse transcriptase, Taq enzyme, RNase inhibitor, and UDG enzyme. Storage Store at -25 to -15℃ in the dark. Valid for 18 months. Notes 1. Please prepare the reaction mixture inside a laminar flow cabinet and use nuclease-free pipette tips and reaction tubes. Filter-tipped pipette tips are recommended to avoid cross-contamination and aerosol contamination. 2. For your safety and health, please wear a lab coat and disposable gloves when operating. 3. For Research Use Only. Instructions 1. Recommended Reaction System Component Volume（μL） Final concentration 2×RT-qPCR Buffer 15 1× 2×RT-qPCR Enzyme Mix 1.2 - Primer/Probe mix (2.5 μM) 3 0.25 μM Template RNA 1-10 - RNase Free H2O Up to 30 - 【Notes】 1) Ensure thorough mixing before use to avoid excessive bubbles from vigorous shaking. 2) The Primer/Probe Mix contains multiple pairs of primers and probes. The final concentration of each primer can be adjusted between 0.1-1.0 μM depending on the situation. The final concentration of the probes can also be adjusted within the range of 0.05-0.5 μM based on the requirements for the fluorescence curve and signal peak. 3) Due to the high sensitivity of qPCR, it is recommended to dilute the template. Aim to keep the Ct values between 20 and 35 for optimal results. 2.  Reference Amplification Program Recycling procedure Temperature Time Cycle Number Reverse transcription 50℃ 20 min 1 Reverse transcription 95℃ 5 min 1 Amplification reaction 95℃ 15 sec 40-45 60℃ 30 sec 【Notes】 1) Reverse transcription can be performed at either 42℃ or 55℃. 2) The amplification reaction temperature should be adjusted according to the Tm value of the designed primers. 3) The required fluorescence signal acquisition time varies among different qPCR instruments. Please set it according to the shortest time limit. 3. Compatible Instruments Applied Biosystems: 5700, 7000, 7300, 7700, 7900HT Fast, StepOne™ , StepOne Plus™ , 7500, 7500 Fast, ViiA™ 7, QuantStudio™ 3 and 5, QuantStudio™ 6,7,12k Flex; Bio-Rad: CFX96, CFX384, iCycler iQ, iQ5, MyiQ, MiniOpticon, Opticon, Opticon 2, Chromo4; Eppendorf: Mastercycler ep realplex, realplex 2 s; Qiagen: Corbett Rotor-Gene Q, Rotor-Gene 3000, Rotor-Gene 6000; Roche Applied Science: LightCycler 480, LightCycler 2.0; Lightcycler 96; Stratagene: MX3000P™, MX3005P™, MX4000P™; Thermo Scientific: PikoReal Cycler; Cepheid: SmartCycler; Illumina: Eco qPCR; SLAN: SLAN-96S,SLAN-96P.  

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Product description This reagent is a 2× qPCR premix system that supports the completion of up to four-channel fluorescence quantitative PCR detection in a single reaction well. The core of the product features a high-performance antibody-mediated hot-start Taq DNA polymerase, which has been genetically engineered to significantly enhance the detection sensitivity and target specificity of the amplification reaction. Specifically optimized for multiplex PCR reactions, the buffer system formulation effectively enhances nucleic acid amplification efficiency, particularly improving the amplification success rate of low-concentration samples. This kit is suitable for molecular detection fields such as gene polymorphism analysis and multiplex target quantitative detection. Specifications Catalog Number N132041E N132041S N132041M Specifications 80 T（12.5 μL/rxn） 400 T（12.5 μL/rxn） 1600 T（12.5 μL/rxn） Components Component Identification Component Name N132041E N132041S N132041M N132041 2×Multiplex qPCR Probe Master Mix 1 mL 5×1 mL 20 mL Storage Store at -25 to -15℃. Valid for 2 years. Notes 1. Please prepare the reaction system inside a laminar flow hood and use pipette tips and reaction tubes that are free of nucleic acid residues. It is recommended to use pipette tips with filters to avoid cross-contamination and aerosol contamination. 2. For your safety and health, please wear a lab coat and disposable gloves when operating. 3. This product is for research use only. Instructions 1. Reaction System   Component Volume（μL） Final concentration 2×Multiplex qPCR Probe Master Mix 12.5 1× Primer mix (10 μM) X 0.1 μM-0.5 μM Probe mix (10 μM) X 50 nM-250 nM Rox reference dye 0.5 1× Template DNA/cDNA 1-10 - ddH2O Up to 25 - 【Notes】 1) Mix thoroughly before use to avoid generating excessive bubbles from vigorous shaking. 2) The Primer Mix contains multiple pairs of primers, with each primer typically at a final concentration of 0.2 µM, which can also be adjusted between 0.1-0.5 µM as needed. 3) The Probe Mix contains multiple probes with different fluorescent signals, and the concentration of each probe can be adjusted between 50-250 nM as required. 4) This product does not contain Rox reference dye. If needed, it is recommended to use the product with catalog number N132049. 5) qPCR is highly sensitive. It is recommended to dilute the template before use. If the template is undiluted cDNA, the template volume should not exceed 1/10 of the total reaction volume. 6) Reaction System: It is recommended to use a reaction volume of 25 µL, 30 µL, or 50 µL to ensure the effectiveness and reproducibility of target gene amplification. 2. Recommended Amplification Protocol Recycling procedure Temperature Time Cycle Number Pre-denaturation 95℃ 5 min 1 Denaturation 95℃ 15 sec 45 Annealing/Extension 60℃ 30 sec 【Note】 1) Amplification Reaction: The amplification reaction temperature should be adjusted according to the Tm value of the designed primers. 2) Fluorescence Signal Acquisition: The required fluorescence signal acquisition time varies among different qPCR instruments. Please set according to the shortest time limit.   Preparation of Quantitative PCR Reaction Mixture Component Volume（μL） Volume（μL） Final concentration 2×miRNA qPCR Master Mix 10 μL 25 μL 1 × Forward Primer（self-provided） X X 400 nM Reverse Primer (10 μM) 0.8 μL 2 μL 400 nM cDNA X X - RNase-free H2O Up to 20 μL Up to 50 μL - 【Note】a. The volume of miRNA first-strand cDNA added should not exceed 1/10 of the total qRT-PCR reaction volume. High concentrations of cDNA may lead to non-specific amplification; it is recommended to dilute the cDNA 10-1000 times as appropriate. b. The Reverse Primer (10 μM) is sourced from Kit N132068. Due to patent confidentiality, the downstream primer sequence for miRNA poly(A) tailing reverse transcription may vary between manufacturers. It is recommended to use Kit N132068 to ensure reliable experimental results. 3.  PCR Reaction Program Setup You can refer to the following two programs for quantitative PCR reactions. 1) Conventional Fluorescent Quantitative PCR Amplification Program (Two-Step Method) Recycling procedure Temperature Time Cycle Number Pre-denaturation 95℃ 10 min 1 Denaturation 95℃ 15 sec 35-40 Annealing/extension 60℃ 20 sec Melting Curve Analysis Default Settings 1 2) Fluorescent Quantitative PCR Fast Amplification Program (Two-Step Method) Recycling procedure Temperature Time Cycle Number Pre-denaturation 95℃ 10 sec 1 Denaturation 95℃ 5 sec 40 Annealing/extension 60℃ 20 sec Melting Curve Analysis Default Settings 1 【Note】The annealing/extension temperature and time can be adjusted according to experimental requirements.The choice between the conventional and fast programs depends on the PCR instrument being used. For example, instruments like the ABI QuantStudio 5 can be set to a fast program, while instruments like the Bio-Rad CFX96 do not have a fast program setting and require the conventional program to be used. 4. Compatible Instruments Applied Biosystems: 5700, 7000, 7300, 7700, 7900HT Fast, StepOne™ , StepOne Plus™ , 7500, 7500 Fast, ViiA™ 7, QuantStudio™ 3 and 5, QuantStudio™ 6,7,12k Flex; Bio-Rad: CFX96, CFX384, iCycler iQ, iQ5, MyiQ, MiniOpticon, Opticon, Opticon 2, Chromo4; Eppendorf: Mastercycler ep realplex, realplex 2 s; Qiagen: Corbett Rotor-Gene Q, Rotor-Gene 3000, Rotor-Gene 6000; Roche Applied Science: LightCycler 480, LightCycler 2.0; Lightcycler 96; Stratagene: MX3000P™, MX3005P™, MX4000P™; Thermo Scientific: PikoReal Cycler; Cepheid: SmartCycler; Illumina: Eco qPCR; SLAN: SLAN-96S,SLAN-96P.

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Nanobody-Tn5 is a fusion protein combining nanobodies with Tn5 transposase, designed to generate anti-mouse Tn5 and anti-rabbit Tn5 that specifically bind to primary antibodies from two different species (mouse and rabbit). When these Nanobody-Tn5 complexes are pre-loaded with adapter sequences containing distinct indexes, the Tn5 enzyme cleaves chromatin and simultaneously inserts the indexed adapters into the fragmented DNA. This allows differentiation between genomic regions targeted by mouse primary antibodies and those targeted by rabbit primary antibodies based on the unique index sequences. This product is a "naked" enzyme and requires user assembly with adapter sequences. Specifications Cat.No. N210605S / N210605M Size 10 μL / 100 μL Components Components No. Name N210605S N210605M N210605-A Anti-mouse Tn5 10 μL 100 μL N210605-B Anti-rabbit Tn5 10 μL 100 μL N210605-C Assemble Buffer 10 μL 100 μL Storage This product should be stored at -85℃~-65℃ for 1 year. Documents： Manual

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NGS cDNA Synthesis Module (gDNA digester plus) is a cDNA synthesis reagent kit for RNA sequencing library preparation compatible with IlluminaTM/MGITM sequencing platforms. It includes reagents for gDNA removal, RNA reverse transcription, and ds-cDNA synthesis. All reagents in this kit have undergone rigorous quality control and functional validation to ensure maximum stability and reproducibility in library preparation. Specifications Cat.No. N210425E / N210425S / N210425M Size 8 T / 24 T / 96 T Components Components No. Name N210425E N210425S N210425M N210425-A DNase Ⅰ Buffer 16 μL 48 μL 192 μL N210425-B DNase I Mix 16 μL 48 μL 192 μL N210425-C 1st Reaction Buffer 48 μL 144 μL 580 μL N210425-D 1st Stand Enzyme Mix 16 μL 48 μL 192 μL N210425-E 2nd Reaction Buffer 56 μL 168 μL 672 μL N210425-F 2nd Strand Enzyme Mix 24 μL 72 μL 288 μL Storage This product should be stored at -25~-15℃ for 1 year. Documents： Manual

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NGS cDNA Synthesis Module is a cDNA synthesis reagent kit for RNA sequencing library preparation compatible with IlluminaTM/MGITM sequencing platforms. It includes highly efficient reagents for RNA reverse transcription and standard double-stranded (ds) cDNA synthesis. The resulting ds-cDNA can be directly used with NGS Flash DNA Library Prep Kit (Enzymatic)(Arcegen Cat#N210004) for downstream library preparation. All reagents in this kit have undergone rigorous quality control and functional validation to ensure maximum stability and reproducibility in library preparation. Specifications Cat.No. N210423E / N210423S / N210423M Size 8 T / 24 T / 96 T Components Components No. Name N210423E N210423S N210423M N210423-A Random Primer 8 μL 24 μL 212 μL N210423-B 1st Reaction Buffer 64 μL 192 μL 1267 μL N210423-C 1st Strand Enzyme Mix 16 μL 48 μL 320 μL N210423-D 2nd Reaction Buffer 56 μL 168 μL 1034 μL N210423-E 2nd Strand Enzyme Mix 24 μL 72 μL 473 μL Storage This product should be stored at -25~-15℃ for 1 year. Documents： Manual

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The NGS DNA Library Prep Kit (Enzymatic) is a next-generation enzymatic library preparation kit compatible with both IlluminaTM and MGITM high-throughput sequencing platforms. Compared to traditional methods, this kit eliminates the need for cumbersome sonication by using high-quality fragmentation enzymes. The fragmentation and end-repair with dA-tailing modules are combined into a single step, significantly reducing the time and cost of library preparation. It is suitable for input amounts ranging from 1 ng to 1 μg of standard plant and animal genomic DNA, microbial genomic DNA, etc. The enzyme produces uniform fragmentation across different species with minimal species-specific variation. DNA fragmentation, end-repair and dA-tailing are all performed in a single tube. The kit is compatible with both IlluminaTM and MGITM adapters and primers for sequencing on both platforms. Applicable to genomic DNA and full-length cDNA input amounts of 1 ng to 1 μg High-quality fragmentation enzyme enables random dsDNA cleavage with minimal bias Fragmentation, end repair, and dA-tailing in a single step High-fidelity enzyme with strong amplification efficiency significantly improves library quality and yield Suitable for a wide range of species Strict batch performance and stability control Specifications Cat.No. N210001E / N210001S / N210001M /N210001L Size 8 T / 24 T / 96 T /96 T(Plate-Packaging） Components Components No. Name N210001E N210001S N210001M N210001L* N210001-A SmearaseTM Buffer 4.0 80 μL 240 μL 960 μL 8×150 μL N210001-B SmearaseTM Enzyme 4.0 80 μL 240 μL 960 μL 8×150 μL N210001-C Ligation Enhancer 4.0 240 μL 720 μL 3×960 μL 8×420 μL N210001-D Rapid DNA Ligase 4.0 80 μL 240 μL 2×480 μL 8×140 μL N210001-E 2×Ultima HF Amplification Mix 200 μL 600 μL 3×800 μL 8×340 μL 【Note】: When using incomplete adapters, no primer mix is required. However, for full-length adapters, a primer mix is essential and must be purchased separately. This kit is compatible with both Illumina™ and MGI™ platforms, but requires platform-specific primer mixes: Cat# N210701 for Illumina™; Cat# N210781 for MGI™. *For plate format (automated 96T), refer to the layout diagram below:      Storage This product should be stored at -25~-15℃ for 1 year. Workflow Documents： Manual Related Blog: Enzymatic Fragmentation DNA Library Prep Kits: Empowering Multi-Disciplinary Research Applications

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NGS DNA Library Prep Kit (Mechanical) is a next-generation library preparation kit specifically developed for IlluminaTM and MGITM high-throughput sequencing platforms. This product utilizes high-quality enzymatic components, improving upon its predecessor by enhancing the efficiency of DNA fragment end repair and A-tailing, as well as optimizing adapter ligation efficiency. The kit employs a novel high-fidelity enzyme, significantly boosting amplification uniformity and fidelity. It can be applied to various samples including routine plant and animal genomes, microbial genomes, FFPE, cfDNA, ChIP DNA, and more, aiding in obtaining excellent sequencing data. Applicable to all DNA samples ranging from 100 pg to 1 μg, including cfDNA and FFPE. Offers high conversion efficiency, achieving library conversion rates above 70%. Verified across multiple samples for excellent library and sequencing outcomes. Undergoes stringent batch performance and stability quality control. Specifications Cat.No. N210005E / N210005S / N210005M Size 8 T / 24 T / 96 T Components Components No. Name N210001E N210001S N210001M N210005-A Endprep Buffer 2.0  56 μL 168 μL 672 μL N210005-B Endprep Enzyme 2.0  24 μL 72 μL 288 μL N210005-C Ligation Enhancer 2.0  240 μL 720 μL 3×960 μL N210005-D Rapid T4 DNA Ligase 2.0 80 μL 240 μL 2×480 μL N210005-E CanaceTM Pro Amplification Mix   200 μL 600 μL 3×800 μL 【Note】: When using short adapters(Incomplete adapter), no primer mix is required. However, for Full-length adapters, a primer mix is essential and must be purchased separately. This kit is compatible with both Illumina™ and MGI™ platforms, but requires platform-specific primer mixes: Cat# N210701 for Illumina™; Cat# N210781 for MGI™. Storage This product should be stored at -25~-15℃ for 1 year. Workflow Documents： Manual

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NGS DNA Selection Beads are based on the SPRI (Solid Phase Reverse Immobilization) principle and, in combination with a carefully optimized buffer system, can be used for DNA fragment size selection and purification during library construction in next-generation sequencing. This product is compatible with DNA and RNA library preparation kits from various brands and has the same usage method as the widely used AMPure XP Beads. Both the fragment recovery efficiency and the library size distribution are highly consistent with those of AMPure XP Beads. Specifications Cat.No. N210362E / N210362S / N210362M / N210362L Size 1 mL / 5 mL / 60 mL / 450 mL Components Name N210362E N210362S N210362M N210362L NGS DNA Selection Beads 1 mL 5 mL 60 mL 450 mL Storage Store at 2~8 °C. Valid for 18 months. Documents： Manual

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NGS dsDNA Methyl Library Prep Kit for IlluminaTM is a double-stranded DNA methylation library preparation kit developed specifically for the IlluminaTM high-throughput sequencing platform. This kit enables the construction of methylation libraries compatible with IlluminaTM sequencing through ligation of specific methylated adapters, followed by bisulfite or enzymatic conversion, and a single amplification step. All components of the kit have undergone rigorous quality control and functional validation to ensure consistent library output. It supports various types of DNA samples, including gDNA, cfDNA, and FFPE-derived DNA. The recommended input DNA amount ranges from 10 ng to 1000 ng, and the kit is compatible with mainstream conversion methods available on the market. Specifications Cat.No. N210602E / N210602S / N210602M Size 8 T / 24 T / 96 T Components Components No. Name N210602E N210602S N210602M N210602-A Endprep Buffer 56 μL 168 μL 672 μL N210602-B Endprep Enzyme 24 μL 72 μL 288 μL N210602-C Ligation Enhancer 240 μL 720 μL 3×960 μL N210602-D Rapid T4 DNA Ligase 80 μL 240 μL 960 μL N210602-E Methyl Stubby Adapter 40 μL 120 μL 480 μL N210602-F 2 × CanaceTM Uracil + PCR Mix 2.0 200 μL 600 μL 2×1200 μL Storage This product should be stored at -25~-15℃ for 1 year. Documents： Manual

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NGS Dual Barcode Fast-Pace DNA Cyclization Kit for MGI is a single-stranded DNA cyclization kit specifically developed and designed for the MGI high-throughput sequencing platform. Composed of high-quality enzymes and an optimized buffer system, this product significantly improves reaction efficiency, enabling the entire cyclization and digestion process to be completed within 30 minutes. This kit is suitable for all PCR-amplified library products with dual barcodes connected according to BGI's standard. Except for limitations of the library construction reagents themselves, this cyclization kit is compatible with all MGI sequencing platforms. Specifications Cat.No. N210420S / N210420M Size 16 T / 96 T Components Components No. Name N210420S N210420M N210420-A DB Splint Oligo 96 μL 576 μL N210420-B Splint Buffer 240 μL 2×720 μL N210420-C Ligase 80 μL 480 μL N210420-D Digestion Buffer 128 μL 768 μL N210420-E Digestion Enzyme 32 μL 192 μL Storage This product should be stored at -25~-15℃ for 1 year. Documents： Manual

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NGS FFPE DNA Repair Reagent is a specialized repair solution designed for FFPE samples, capable of repairing various degrees of damage in low-quality FFPE samples, thereby improving library yield and sequencing quality. This product can be directly used with Arcegen NGS DNA Library Preparation Kits (Mechanical) (Cat#N210005) and the A-tailing module (Cat#N210417). The system is fully compatible and suitable for repairing 5 ng – 1 μg of fragmented FFPE DNA. Additionally, this product is also compatible with the Arcegen enzymatic fragmentation-based NGS DNA Library Prep Kit (Enzymatic)(Cat#N210001).  Specifications Cat.No. N210416S / N210416M Size 24 T / 96 T Components Components No. Name N210416S N210416M N210416-A FFPE DNA Repair Mix 96 μL 384 μL N210416-B Endprep Buffer 2.0 144 μL 576 μL Storage This product should be stored at -25~-15℃ for 2 years. Notes 1. For your safety and health, wear a lab coat and disposable gloves when handling this product. 2. Place all kit components on ice for thawing before use. After thawing, invert the tubes several times to ensure thorough mixing, briefly centrifuge, and then keep on ice until use. 3. When preparing reaction mixtures, gently pipette or vortex to mix. Avoid vigorous vortexing, which may reduce library yield. 4. To prevent cross-contamination between samples, use filter tips and change pipette tips when handling different samples. 5. It is recommended to perform all reaction steps in a thermal cycler with a heated lid. Pre-warm the thermal cycler to the reaction temperature prior to use. 6. This product is intended for research use only! Documents： Manual

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The NGS Flash DNA Library Prep Kit(Enzymatic) is a fast enzymatic library preparation kit. This product utilizes high-quality fragmentation enzymes that integrate the fragmentation with the end repair & A-tailing module, streamlining the workflow and significantly reducing the time and cost of library preparation. It is suitable for input amounts of 100 pg–500 ng of conventional plant and animal genomic DNA, microbial genomic DNA, etc., and allows rapid fragmentation, end repair, and A-tailing reactions to be completed in a single tube. The kit is compatible with both IlluminaTM and MGITM adapters and primers for sequencing on both platforms. Suitable for 100 pg–500 ng genomic DNA samples Compatible with IlluminaTM and MGITM high-throughput sequencing platforms Fragmentation and end repair completed within 5 minutes High library conversion rate and amplification efficiency Specifications Cat.No. N210004S / N210001 M Size 24 T / 96 T Components Components No. Name N210004E N210004S N210004-A SmearaseTM Mix 240 μL 960 μL N210004-B Ligation Enhancer 720 μL 4×720 μL N210004-C Fast T4 DNA Ligase 120 μL 480 μL N210004-D 2×Ultima HF Amplification Mix 600 μL 4×600 μL * Primer Mix* 120 μL 480 μL 【Note】: When using short adapters(Incomplete adapter), no primer mix is required. However, for Full-length adapters, a primer mix is essential and must be purchased separately. This kit is compatible with both Illumina™ and MGI™ platforms, but requires platform-specific primer mixes: Cat# N210701 for Illumina™; Cat# N210781 for MGI™. Storage This product should be stored at -25~-15℃ for 1 year. Workflow Documents： Manual

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The NGS Fragmentation, End repair and dA-Tailing Module is a next-generation enzyme-based library preparation kit designed for high-throughput sequencing platforms from IlluminaTM and MGITM. This product utilizes high-quality fragmentation enzymes to eliminate the cumbersome ultrasonication process, simplifying the operation procedure by combining the fragmentation module with end-repair/dA-tailing into one. It significantly reduces library preparation time and costs. Suitable for 500 pg-1 μg of typical plant and animal genomic DNA, microbial genomes, etc., it enables DNA fragmentation, end repair, and dA tailing reactions in a single tube. The reaction products can be directly used for adapter ligation using the NGS Novel DNA Ligation Module(Cat#N210426). Specifications Cat.No. N210418S / N210418M Size 24 T / 96 T Components Components No. Name N210418S N210418M N210418-A SmearaseTM Buffer 240 μL 960 μL N210418-B SmearaseTM Enzyme 120 μL 480 μL Storage Shipped with dry ice.This product should be stored at -25~-15℃ for 18 months. Documents： Manual

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NGS Library Amplification Mix for Library Amplification is a ready-to-use 2× premixed solution containing High-Fidelity DNA Polymerase (with fidelity six times higher than ordinary pfu DNA polymerase and an amplification speed of 15 sec/kb), dNTPs, and a buffer system meticulously optimized for high-throughput sequencing library amplification. It offers rapid and simple operation, high sensitivity, strong specificity, and excellent stability. During the library amplification reaction, only primers and templates need to be added to the mix, simplifying experimental steps, reducing human error, and improving experimental throughput and reproducibility. Additionally, this product contains a special stabilizer that maintains activity even after repeated freeze-thaw cycles. This product has been validated through successful use with the NGS End repair and dA-Tailing Module(Cat#N210417) and NGS Novel DNA Ligation Module(Cat#N210426) for DNA library construction, followed by sequencing on the IlluminaTM high-throughput platform. All reagents provided in this product undergo strict quality control to ensure optimal performance and batch-to-batch consistency. Specifications Cat.No. N210501S / N210501M Size 24 T / 96 T Product Applications Gene cloning; amplification of complex DNA templates; high-throughput library amplification. Storage Shipped with ice packs.This product should be stored at -25℃~-15℃ for 18 months. Notes 1. For your safety and health, wear lab coats and disposable gloves during operation. 2. This product is for research use only! Documents： Manual

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The NGS mRNA Isolation Master Kit is an upgraded magnetic bead-based reagent kit specifically designed for the purification of mRNA. The mRNA Capture Beads in the kit are micrometer-sized superparamagnetic microspheres conjugated with Oligo(dT), which specifically bind to the poly(A) tails of mRNA molecules. This enables the efficient isolation and purification of mRNA from 10 ng to 4 μg of high-quality total RNA. Specifications Cat.No. N210361S / N210361M / N210361L Size 8T / 24 T / 96 T Components Components No. Name N210361S N210361M N210361L N210361-A mRNA Capture Beads 2.0 400 μL 1.2 mL 4.8 mL N210361-B Beads Binding Buffer 2.0 400 μL 1.2 mL 4.8 mL N210361-C Beads Wash Buffer 2.0 5 mL 15 mL 60 mL N210361-D Tris Buffer 2.0 400 μL 1.2 mL 4.8 mL N210361-E Nuclease-free water 1 mL 1 mL 4 mL Storage Store at 2–8 °C.Valid for 1 year. Documents： Manual

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NGS PreMix RNA Library Prep Kit(Strand-specific) is a strand-specific RNA sequencing library preparation kit compatible with both IlluminaTM and MGITM platforms. This product is available in standard tube format and pre-loaded plate format. The pre-loaded plate format eliminates the need for manual reagent preparation or aliquoting, and can be directly adapted for automated library preparation, offering greater convenience. The kit includes all necessary reagents for RNA fragmentation, reverse transcription, strand-specific ds-cDNA synthesis, and library amplification. It is compatible with upstream workflows involving mRNA enrichment or rRNA depletion. Engineered for enhanced user safety and data quality, the kit features an optimized reverse transcription system that achieves high strand specificity without the use of actinomycin D. Every component undergoes stringent quality control and functional testing to ensure robust library prep performance, batch-to-batch consistency, and high reproducibility. Specifications Cat.No. N210104S / N210104M / N210104MP Size 24 T / 96 T / 96 T (plate) Components Components No. Name N210104S N210104M N210104MP** N210104-A Frag/Primer Buffer 450 μL 2×900 μL 8×266 μL N210104-B 1st Reaction Module 2.0 192 μL 768 μL 8×120 μL N210104-C 2nd Reaction Module(dUTP) 840 μL 3×1120 μL 8×480 μL N210104-D Ligation Reaction Module 840 μL 3×1120 μL 8×480 μL N210104-E 2×Super CanaceTM II High-Fidelity Mix 600 μL 2×1200 μL 8×340 μL * Primer Mix* / / / [Note]:  * This component is not included in the kit. It is not required when using short or incomplete adapters. However, if full-length or complete adapters are used during library preparation, this component must be added separately. This kit is compatible with both IlluminaTM and MGITM platforms. Platform-specific primer mixes must be purchased separately: Cat# N210782 — RNA Library Prep Primer Mix for MGITM (compatible with complete adapters) Cat# N210701 — Primer Mix for IlluminaTM ** For the layout of reagents in the pre-loaded plate format, please refer to the figure below. The left image shows the reagent layout of the pre-loaded plate format library preparation kit, with each component occupying one column, and each well containing sufficient reagent for 12 library preparation reactions. When using the pre-loaded plate kit with automated instruments, the empty plate positions (columns 6-12) can be filled with beads, adapters, or other reagents according to experimental needs prior to the experiment. It is recommended that each well of the pre-loaded plate format reagent be used up in a single use.  Figure 1. Layout of Reagent Components for Plate-based Library Preparation Kit Storage This product should be stored at -25~-15℃ for 1 year. Documents： Manual Related Blog: The Ultimate Guide to NGS mRNA Library Prep: From mRNA Purification to Key Tips

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NGS PreMix RNA Library Prep Kit(dNTP) is a total RNA sequencing library preparation kit compatible with both IlluminaTM and MGITM platforms. This product is available in standard tube format and pre-loaded plate format. The pre-loaded plate format eliminates the need for manual reagent preparation or aliquoting, and can be directly adapted for automated library preparation, offering greater convenience. The kit includes all necessary reagents for RNA fragmentation, reverse transcription, strand-specific ds-cDNA synthesis, and library amplification. It is compatible with upstream workflows involving mRNA enrichment or rRNA depletion. Engineered for enhanced user safety and data quality, the kit features an optimized reverse transcription system that achieves high strand specificity without the use of actinomycin D. Every component undergoes stringent quality control and functional testing to ensure robust library prep performance, batch-to-batch consistency, and high reproducibility. Specifications Cat.No. N210105S / N210105M / N210105MP Size 24 T / 96 T / 96 T (plate) Components Components No. Name N210105S N210105M N210105MP** N210105-A Frag/Primer Buffer 450 μL 2×900 μL 8×266 μL N210105-B 1st Reaction Module 2.0 192 μL 768 μL 8×120 μL N210105-C 2nd Reaction Module(dNTP) 840 μL 3×1120 μL 8×480 μL N210105-D Ligation Reaction Module 840 μL 3×1120 μL 8×480 μL N210105-E 2×Super CanaceTM II High-Fidelity Mix 600 μL 2×1200 μL 8×340 μL * Primer Mix* / / / [Note]: * This component is not included in the kit. It is not required when using short or incomplete adapters. However, if full-length or complete adapters are used during library preparation, this component must be added separately. This kit is compatible with both IlluminaTM and MGITM platforms. Platform-specific Primer mixes must be purchased separately: Cat# N210782 — RNA Library Prep Primer Mix for MGITM (compatible with complete adapters) Cat# N210701 — Primer Mix for IlluminaTM ** For the layout of reagents in the pre-loaded plate format, please refer to the figure below. The left image shows the reagent layout of the pre-loaded plate format library preparation kit, with each component occupying one column, and each well containing sufficient reagent for 12 library preparation reactions. When using the pre-loaded plate kit with automated instruments, the empty plate positions (columns 6-12) can be filled with beads, adapters, or other reagents according to experimental needs prior to the experiment. It is recommended that each well of the pre-loaded plate format reagent be used up in a single use.  Figure 1. Layout of Reagent Components for Plate-based Library Preparation Kit Storage This product should be stored at -25~-15℃ for 1 year. Documents： Manual

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This kit utilizes a unique magnetic bead and buffer system that specifically binds RNA, effectively removing proteins, salt ions, and other contaminants. It is commonly used for the purification of total RNA samples after rRNA depletion, in vitro transcribed RNA, labeled RNA products, and synthetic RNA. The purified RNA is suitable for downstream applications such as RNA library construction, RT-PCR, qRT-PCR, microarray analysis, Northern blot, and RNA interference (RNAi). Specifications Cat.No. N210363E / N210363S / N210363M / N210363L Size 1 mL / 5 mL / 60 mL Components Name N210363E N210363S N210363M N210363L NGS RNA Purification Beads 1 mL 5 mL 60 mL 450 mL Storage Store at 2–8 °C. Valid for 2 years. Documents： Manual

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NGS rRNA Depletion Kit ( Human/Mouse/Rat) utilizes an RNase H digestion method to remove ribosomal RNA (rRNA) and the 45S & ITS/ETS regions from total RNA isolated from human, mouse, and rat samples, thereby enriching messenger RNA (mRNA) and other non-coding RNAs. This kit effectively removes rRNA from both intact and partially degraded total RNA (such as FFPE-derived RNA). Since degraded FFPE samples typically contain a higher proportion of ITS/ETS fragments compared to fresh tissue samples, the inclusion of specific probes targeting the human, mouse, and rat 45S & ITS/ETS regions in this kit significantly increases the proportion of usable sequencing data after depletion. Specifications Cat NO. N210402E / N210402S / N210402M Size 12 T/24 T /96 T Components Components No. Name N210402E N210402S N210402M N210402-A Hybridization Buffer 36 μL 72 μL 288 μL N210402-B Human Probe Mix (rRNA & ITS/ETS) 24 μL 48 μL 192 μL N210402-C RNase H Buffer 36 μL 72 μL 288 μL N210402-D Thermostable RNase H 24 μL 48 μL 192 μL N210402-E DNase I Buffer 330 μL 660 μL 2×1320 μL N210402-F DNase l 30 μL 60 μL 240 μL Storage This product should be stored at -25~-15℃ for 1 year. Notes 1. Use RNase-free consumables and regularly clean work areas. It is recommended to use Thermo Fisher’s RNAZapTM efficient nucleic acid removal spray to eliminate RNase contamination. 2. RNA samples should be free of genomic DNA (gDNA) contamination. If gDNA is present, perform DNase I digestion followed by purification prior to using this kit. 3. The maximum input volume for RNA samples is 10 μL. If the sample volume exceeds this, concentrate the sample before use. 4. If preparing a mix for the RNase H digestion step, prepare it fresh and use immediately. 5. For your safety and health, wear a lab coat and disposable gloves during operation. 6. This product is intended for research use only! Documents： Manual

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Product description NGS rRNA Depletion Kit (Plant) utilizes RNase H digestion to remove ribosomal RNA (including cytoplasmic 18S, 25S rRNA, mitochondrial 18S, 26S rRNA, and chloroplast 16S, 23S rRNA) from total RNA of plant origin, thereby preserving messenger RNA (mRNA) and other non-coding RNA. This kit provides effective rRNA removal for both intact and partially degraded total RNA samples. The RNA samples obtained after rRNA depletion can be used for high-throughput sequencing to analyze mRNA and non-coding RNA, significantly increasing the proportion of usable sequencing data. They can also be used for cDNA synthesis or other downstream applications. Application Suitable for total RNA samples from various plant sources ranging from 100 ng to 1 μg; suitable for both intact and partially degraded RNA samples. Specifications Cat.No. N210401E / N210401S / N210401M Size 12 T / 24 T / 96 T Components Components No. Name N210401E N210401S N210401M N210401-A Hybridization Buffer 36 μL 72 μL 288 μL N210401-B Probe Mix (Plant) 36 μL 72μL 288 μL N210401-C RNase H Buffer 36 μL 72 μL 288 μL N210401-D Thermostable RNase H 24 μL 48 μL 192 μL N210401-E DNase I Buffer 330 μL 660 μL 2×1320 μL N210401-F DNase I 30 μL 60 μL 240 μL Storage Shipped on dry ice, store at -25~-15 °C.Valid for one year. Notes 1. Please use RNase-free consumables and regularly clean the work area. Thermo Fisher Scientific’s RNAZap™ High-Efficiency Nuclease Removal Spray is recommended for RNase contamination removal. 2. RNA samples should be free of genomic DNA contamination. If gDNA is present, perform DNase I digestion followed by purification before using this kit. 3. The maximum input volume for RNA samples is 10 μL. If the sample volume exceeds this, concentrate the RNA before use. 4. If the RNase H digestion step requires a reaction mix, please prepare it freshly before use. 5. For personal safety and health, wear a lab coat and disposable gloves during operation. 6. This product is for research use only. Documents： Manual

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Product description NGS Single Barcode Fast-Pace DNA Cyclization Kit for MGI is a single-stranded DNA cyclization kit specifically designed for the MGI high-throughput sequencing platform. Composed of high-quality enzymes and an optimized buffer system, this product significantly enhances reaction efficiency, enabling the entire cyclization and digestion process to be completed within 30 minutes. This kit is suitable for amplification products of all single-index PCR libraries constructed according to BGI's standard protocols. Except for limitations imposed by the library construction reagents themselves, this cyclization kit is not restricted to any specific MGI sequencing platform. Specifications Cat.No. N210421S / N210421M Size 16 T / 96 T Components Components No. Name N210421S N210421M N210421-A Splint Oligo 96 μL 576 μL N210421-B Splint Buffer 240 μL 2×720 μL N210421-C Ligase 80 μL 480 μL N210421-D Digestion Buffer 128 μL 768 μL N210421-E Digestion Enzyme 32 μL 192 μL Storage This product should be stored at -25~-15℃ for 1 year. Documents： Manual

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NGS T4 DNA Ligase (400 U/μL) is a single enzyme product suitable for the ligation of DNA fragments and adapters during NGS library preparation. This product has been validated through high-throughput sequencing, demonstrating superior quality. For users who do not require adjustments to the system, it is recommended to choose the NGS Novel DNA Ligation Module (Cat#N210426). Specifications Cat.No. N210504S / N210504M Size 40 KU / 400 KU Components Components No. Name N210504S N210504M N210504-A Quick T4 DNA Ligase (400 U/µL) 100 µL 1 mL N210504-B 10 × T4 DNA Ligase Buffer 250 µL 2×1250 µL Storage Shipped with ice packs.This product should be stored at -25℃~-15℃ for 2 years. Unit Definition One unit (U) is defined as the amount of enzyme required to catalyze the ligation of over 50% of 6 μg λDNA-Hind Ⅲ fragments in a 20 μL reaction volume at 16°C within 30 minutes. Notes 1. A small amount of sediment may appear normal when buffers are thawed. Mix thoroughly by inversion before use. 2. For your safety and health, wear lab coats and disposable gloves while operating. 3. This product is for research purposes only! Documents： Manual

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NGS Total RNA Library Prep Kit is a total RNA sequencing library preparation kit compatible with both Illumina and MGI platforms. It includes reagents for RNA fragmentation, reverse transcription, conventional and strand-specific double-stranded cDNA (ds-cDNA) synthesis, and library amplification. It can be used in conjunction with mRNA purification kits or rRNA depletion kits to prepare sequencing libraries. The second-strand synthesis module contains two types of buffers, allowing users to choose between conventional or strand-specific library preparation. In the strand-specific second-strand synthesis buffer, dTTP is replaced with dUTP, enabling dUTP incorporation into the second cDNA strand. The high-fidelity DNA polymerase used in this kit cannot amplify DNA templates containing uracil, thereby achieving strand specificity. All reagents included in the kit have undergone rigorous quality control and functional validation to ensure maximum library preparation stability and reproducibility. Specifications Cat.No. N210102S / N210102M Size 24 T / 96 T Components Components No. Name N210102S N210102M N210102-A 2× Frag/Prime Buffe 250 μL 930 μL N210102-B 1st Strand Enzyme Mix 48 μL 192 μL N210102-C Strand Specificity Reagent 150 μL 580 μL N210102-D 2nd Strand Buffer (dNTP) 720 μL 2×1440 μL N210102-E 2nd Strand Buffer (dUTP) 720 μL 2×1440 μL N210102-F 2nd Strand Enzyme Master Mix 120 μL 480 μL N210102-G Ligation Enhancer 720 μL 2×1440 μL N210102-H Novel T4 DNA Ligase 120 μL 480 μL N210102-I 2 × Super CanaceTM II High-Fidelity Mix 600 μL 2×1200 μL N210102-K Nuclease Free H2O 300 μL 1000 μL N210102-* Primer mix NA NA 【Note】: When using short adapters(Incomplete adapter), no primer mix is required. However, for Full-length adapters, a primer mix is essential and must be purchased separately. This kit is compatible with both Illumina™ and MGI™ platforms, but requires platform-specific primer mixes: Cat# N210701 for Illumina™; Cat# N210781 for MGI™. Storage This product should be stored at -25~-15℃ for 1 year. Documents： Manual

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NGS Uracil+ Library Amplification Mix is a ready-to-use 2× premix solution containing Hieff CanaceTM Uracil+ High-Fidelity DNA polymerase, dNTPs, and an optimized buffer system specifically tailored for high-throughput sequencing library amplification. It offers high sensitivity, strong specificity, and excellent fidelity. During amplification reactions, users only need to add primers and template to the mixture, simplifying experimental procedures, reducing human error, and improving throughput and reproducibility of results. All reagents are rigorously quality-controlled to ensure superior performance and batch-to-batch consistency. Specifications Cat.No. N210419E / N210419S / N210419M Size 1 mL / 5 mL / 25 mL Storage This product should be stored at -25~-15℃ for 18 months. Documents： Manual

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The NTP Set Solution is a convenient set of 100 mM aqueous solutions of each of ATP, CTP, UTP, GTP, supplied in separate vials. They can be used in a variety of molecular biology applications, such as in vitro transcription, RNA amplification, siRNA synthesis, etc, additionally, used as a reaction substrate or coenzyme for a variety of enzymes. This product is a clear colorless solution prepared from trisodium salts of ATP, UTP, GTP and CTP with a purity of ≥99%, pH = 7.0±0.1 (25°C), concentration of 100 mM, and is DNase-free and RNase-free.   Components Component Number  Components  Size N120006-A ATP (100 mM) 1 mL N120006-B UTP (100 mM) 1 mL N120006-C CTP (100 mM) 1 mL N120006-D GTP (100 mM) 1 mL Shipping and Storage Shipped on dry ice. Store at -15℃ ~ -25℃ for two years.  

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Product description This nucleic acid stain has been validated by the Ames test to be completely non-mutagenic at gel staining concentrations, serving as a novel, non-toxic nucleic acid dye. It is a safe alternative to ethidium bromide (EB), retaining EB-equivalent thermal stability, sensitivity, and UV spectral properties. Detection can be performed under 300 nm UV excitation, and it is suitable for staining dsDNA, ssDNA, and RNA in both agarose and polyacrylamide gel electrophoresis (PAGE). Compatible with in-gel staining or solution-based staining methods, it offers flexible application and delivers high-resolution detection. Components Components No. N132109S Size 500 μL Shipping and Storage Store at room temperature protected from light, valid for five years. Notes 1. If high molecular weight bands exhibit smearing or poor resolution, it is recommended to reduce the loading amount of DNA marker or nucleic acid samples. 2. In-gel staining is not suitable for precast polyacrylamide gels. For polyacrylamide gels, use solution-based staining methods. 3. For your safety and health, please wear a lab coat and disposable gloves. 4. For research use only！ Instructions 1. In-Gel Staining (similar to EB, pre-electrophoresis staining) 1）Prepare agarose gel at the desired concentration and heat in a microwave until fully melted. 2）Add nucleic acid stain at a final concentration of 1× (i.e., add 5 μL of 10,000× aqueous nucleic acid stain per 50 mL agarose solution). Mix gently. 3）Pour the agarose solution containing the stain into a gel-casting tray, insert a comb, and allow to solidify at room temperature for 30-60 min. 4）Load samples and perform electrophoresis using standard protocols. 5）Image under UV light. 【Note】This stain exhibits excellent thermal stability. The dye may also be added directly to the electrophoresis buffer containing agarose powder, followed by heating via microwave or conventional methods to prepare the gel. 2. Post-Electrophoresis Staining (solution-based staining) 1）Prepare agarose gel at the desired concentration and heat in a microwave until fully melted. 2）Pour the agarose solution into a gel-casting tray, insert a comb, and allow to solidify at room temperature for 30-60 min. 3）Load samples and perform electrophoresis using standard protocols. 4）Dilute the 10,000×aqueous nucleic acid stain to a 3×working solution using 0.1 M NaCl (i.e., add 15 μL of 10,000×stain to 50 mL of 0.1 M NaCl). This working solution can be reused up to 3 times and stored at room temperature protected from light. 5）Place the gel in a suitable container, submerge it in the 3×staining solution, and stain with gentle agitation at room temperature for about 30 min. 【Note】Optimal staining time depends on gel thickness and concentration. For 3.5-10% polyacrylamide gels, staining typically requires 30 min to 1 h, with longer times needed for higher polyacrylamide content. 6）Image under UV light.

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Product description This kit is a new generation homologous recombinant cloning kit. The carefully optimized 2nd generation 2 × Clone Enzyme Premix combines the recombinant enzyme and the buffer required for the recombinant reaction with the addition of a unique recombinant enhancer to significantly improve the efficiency of recombinant cloning. The kit can be directed to clone PCR products to any site of any vector, compatible with unpurified PCR products, directly recovered PCR products, low concentration of rubber recovered products, this product can reassemble the homologous arm GC content of 30%-70% of the joint fragment. The vector was completely linearized, and homologous sequences of the end of the linearized vector of 15-25 bp were introduced into the 5 'end of the positive and reverse PCR primers of the inserted fragment, so that the 5' and 3 'ends of the PCR products of the inserted fragment had exactly the same sequence corresponding to the two ends of the linearized vector, respectively. Under the action of recombinant enzyme, the recombinant reaction of PCR product and linearized vector can be completed in as little as 5 min at 50℃. The positive rate of cloning can reach more than 95%. Components Components No. Name N132081S (20 T) N132081M (50 T) N132081-A 2 × Clone Enzyme Premix 200 μL 500 μL N132081-B 500 bp control insert (25 ng/μL) 5 μL 5 μL N132081-C pUC 19 control vector, linearized (50 ng/μL) 5 μL 5 μL Shipping and Storage Dry ice shipping. -15℃ ~ -25℃ storage, valid for one year. Notes 1. Materials to be prepared by oneself: 1) Sample: Prepare the linearized vector and insert fragments by oneself. 2) Self-prepared reagents (only some of them are listed) : a. super receptive cells: conversion efficiency > 108 cfu/μg, such as DH5α Fast Chemically Competent Cell. b. High fidelity enzyme: 2×High-Fidelity Fast PCR Master Mix (With Dye)（Cat#N132016） or other equivalent. c. Colony PCR mix: 2×HotStart Fast PCR Master Mix (With Dye)（Cat#N132001）or other equivalent product. d. Nucleic Acid dye: Nucleic Acid Gel Stain (10,000× in Water)（Cat#N132109） or other equivalent products. 3) Self-supplied instrument consumables (only listed part): PCR instrument, horizontal electrophoresis tank, glue cutting instrument, EP tube, etc. 2. For your safety and health, please wear lab coats and disposable gloves for operation. 3. This product is for research use ONLY! Instructions 1. Preparation of target vector and insert fragment 1) Preparation of linearized vector Select the appropriate cloning site and linearize the vector. This product is not affected by GC content in the homologous arm region. Linearized vectors can be obtained by restriction enzyme digestion or reverse PCR amplification. a. Enzyme digestion preparation of linearized vector * Double enzyme digestion linearization: complete linearization, low conversion background. Recommended. Single digestion linearization: the degree of linearization is poor. The time of enzyme digestion can be extended appropriately to reduce the conversion background. 【Note】:* False-positive clones without inserted fragments may be formed by the transformation of incomplete linearized circular vectors. If the proportion of such false-positive clones is high, it is recommended to prepare the linearized vectors again and cut the glue for recovery. b. Linearized vector was prepared by reverse PCR amplification Vector amplification using a high-fidelity polymerase (such as 2×High-Fidelity Fast PCR Master Mix (With Dye), Cat#N132016) is recommended to reduce the introduction of amplification mutations. The PCR amplification template should use pre-linearized plasmids as much as possible to prevent the influence of residual ring plasmids on the positive rate of cloning. The recombinant reaction system is compatible with almost all enzyme digestion reaction systems and conventional PCR reaction systems. When the purity of vector enzyme digestion products or reverse PCR amplified products is high, the recombinant reaction can be directly carried out without purification. However, when the purity is low and may contain unlinearized ring plasmids, it is recommended to use a high-quality kit for rubber recovery and purification of the linearized vector to improve the purity of the product and remove part of the unlinearized ring vector, which is conducive to improving the recombinant efficiency. 2) Primer design for a single insert fragment Primer design method: The 5 'and 3' ends of the PCR products of the inserted fragment have identical sequences corresponding to the two ends of the linearized vector, respectively, by introducing 15-25 bp homologous sequences of the 5 'ends of the positive and reverse PCR primers of the inserted fragment. l Insertion fragment forward amplification primer design method: 5 '-upstream vector end homologous sequence + enzyme restriction site (can be retained or deleted) + gene specific forward amplification primer sequence -3' l Insertion fragment reverse amplification primer design method: 3 '- gene-specific reverse amplification primer sequence + cleavage site (can be retained or deleted) + downstream vector terminal homologous sequence -5'   It is recommended to use mature cloning software for primer design. The final primer length is more than 40 bp, and PAGE purification method is recommended for primer synthesis. When calculating the annealing temperature of the amplification primer, only the Tm value of the gene-specific amplification sequence should be calculated, and the homologous sequence at the end of the vector should not be involved in the calculation. In order to obtain high efficiency cloning, it is recommended that Tm≥48℃. 3) Primer design for multiple insertion fragments Primer design method: The 5 'and 3' ends of the PCR products of the inserted fragment have identical sequences corresponding to the two ends of the linearized vector, respectively, by introducing 15-25 bp homologous sequences of the 5 'ends of the positive and reverse PCR primers of the inserted fragment. It is recommended to use mature cloning software to automatically generate insert fragment amplification primer. For manual design, refer to the following example. Take the primer design of three gene segments inserted between the EcoR I and Hind III cleavage sites of pUC18 vector as an example, the specific design scheme of the primer is as follows: a. First design the forward amplification primers of the first fragment and the reverse amplification primers of the third fragment (two inserted fragments adjacent to the vector). l First fragment forward amplification primer design: 5 '-upstream vector terminal homologous sequence + enzyme restriction site (can be retained or deleted) + first fragment gene specific forward amplification sequence -3' l Third fragment reverse amplification primer design method: 3 '-third fragment gene specific reverse amplification sequence + enzyme cut site (can be retained or deleted) + downstream vector terminal homologous sequence -5' b. Secondly, the reverse amplification primers of the first fragment and the forward amplification primers of the second fragment were designed. Homologous sequences for recombination between fragments may be added to the reverse amplification primer of the anterior fragment, to the forward amplification primer of the posterior fragment, or to a part of each fragment. Take the example of adding a homologous sequence to a reverse amplification primer of the front fragment (the first fragment) : l First fragment reverse amplification primer design: 3 '-first fragment gene-specific reverse amplification sequence + second fragment 5' terminal homologous sequence -5 ' l Second fragment forward amplification primer design method: 5 '-second fragment gene specific forward amplification sequence -3' c. Finally, the reverse amplification primers of the second fragment and the forward amplification primers of the third fragment were designed. The design method is consistent with the reverse amplification primers of the first fragment and the forward amplification primers of the second fragment. 4) PCR amplification of the inserted fragment The insertion fragment amplification can be amplified by any PCR enzyme, regardless of whether there is an A-tail at the end of the product (which will be removed during the recombination process and will not be present in the final vector). However, in order to reduce the introduction of amplification mutations, it is recommended to amplify With a high-fidelity polymerase (e.g. 2×High-Fidelity Fast PCR Master Mix (With Dye)(Cat#N132016). It is recommended to use the mature cloning software to automatically generate insert fragment amplification primer. After PCR amplification, a small amount of the product was taken for agarose gel electrophoresis to test the amplification yield and specificity. The recombinant reaction system is compatible with the conventional PCR reaction system. Therefore, if the amplification template is not a circular plasmid with the same resistance as the vector, and the electrophoresis band of the PCR product is single, the amplified product can be directly used in the recombination reaction without purification. However, when the purity of PCR amplification products is low, it is recommended to use high quality kit for rubber recovery and purification of PCR amplification products to improve the purity of products and improve the efficiency of recombinant. 2. Concentration measurement The method of band brightness comparison by agarose gel electrophoresis is recommended for DNA quantification. The linearized vector and the inserted fragment amplification product were subjected to several equal volume dilution gradients. The original product and the diluted product were each taken 1 μL for agar-gel electrophoresis. The band brightness was compared with DNA Marker to determine the approximate concentration (especially in the case of unpurified linearized vector and inserted fragment amplification product). Nucleic acid concentration meter can also be used to determine the linearized vector and the inserted fragment, record the concentration value (ng/μL) and OD260/OD280 values, and then calculate the amount of input.

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phi29 DNA polymerase is derived from the Bacillus subtilis bacteriophage. After genetic engineering, it exhibits excellent strand displacement and processive synthesis capabilities, enabling the synthesis of DNA fragments up to 70 kb in length. Additionally, it possesses strong 3’→5’ exonuclease proofreading activity. Primer 3’ end modifications are recommended in the recommended reaction system to prevent degradation. This enzyme is commonly used in in vitro plasmid synthesis and whole genome amplification. Specifications Cat.No. N210508S / N210508M / N210508L Size 250 U / 1000 U / 5000 U Unit Definition The amount of enzyme required to incorporate 0.5 pmol of dNTP into acid-insoluble material in 10 minutes at 30°C. Heat Inactivation 65°C for 10 min. Components Components No. Name N210508S N210508M N210508L N210508-A phi29 DNA Polymerase (10 U/μL） 25 μL 100 μL 500 μL N210508-B 10×phi29 Reaction Buffer 100 μL 400 μL 2×1 mL Storage This product should be stored at -25~-15℃ for 2 years. Documents： Manual

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Product description Plant tissue direct PCR kit is a kit that can directly amplify different types of plant leaves by PCR, with wide adaptability and strong stability. The kit uses a unique lysis buffer system, which can quickly lyse a variety of plant samples and release genomic DNA. The released genomic DNA can be used directly as a template without removing protein, RNA, or secondary metabolites in PCR reaction. In addition, the kit requires a small amount of sample, as low as 1 mm plant leaves can be used for experiments. The 2×Plant Master Mix provided in this kit has strong amplification compatibility and can directly use the lysate of the sample as a template for efficient and specific amplification. This reagent is a 2-fold concentrated PCR reaction mixture, which contains all the components used for PCR amplification except the template and primers, which greatly simplifies the operation process and reduces the chance of contamination. The kit can be used for identification of transgenic plants, plant genotyping, etc. Components Components No. Name N132023E (50 T) N132023S  (200 T) Storage N132023-A Buffer P1 2×1.25 mL 2×5 mL 2-8℃ N132023-B Buffer P2 500 μL 2×1 mL 2-8℃ N132023-C 2×Plant Master Mix* 500 μL 2×1 mL -25 ~ -15℃ 【Note】: *2×Plant Master Mix: Contains hot-start Taq DNA polymerase, dNTP mix, MgCl2, reaction buffer, PCR reaction enhancer, optimizer and stabilizer, etc. It also includes electrophoresis Loading Buffer, which can be directly used in electrophoresis after PCR. Shipping and Storage 1. Component A: The product should be stored at 2-8℃ for one year. For multiple use for a long time. 2. Component B: The product should be stored at 2-8℃ for one year. It is used for neutralizing lysate, which is beneficial to store the sample for a longer time. 3. Component C: The product should be stored at -25 ~ -15℃ for one year. Please avoid repeated freeze-thaw. Notes 1) When doing leaf experiments, it is recommended to use freshly collected leaf tissue. If it is a long-term frozen tissue, it needs to be stored at -80℃. Repeated freezing and thawing should be avoided as much as possible to avoid template degradation and affect PCR efficiency. The leaf tissue is suitable for young leaves. If it is a mature leaf, avoid using the tissue of the main vein of the leaf. 2) It is recommended to amplify the fragment within 1 kb in length for the best amplification efficiency. 3) When sampling, use a hole punch or knife to take a sample of suitable size. When the samples are different, the hole punch or knife needs to be cleaned every time before processing the sample. 4) For leaf tissue, it is recommended to take 1-10 mm leaves, too small leaf length will lead to low PCR amplification yield, too much will inhibit the PCR reaction, use the method of thermal cracking, mashing with a pipette tip, and crushing with a grinder to treat plant leaves. After treatment, it needs to be shaken and centrifuged. Be sure to take the supernatant for testing. Precipitation will seriously inhibit the PCR reaction. 5) For your safety and health, please wear lab coats and disposable gloves for operation. 6) This product is for research use ONLY! Instructions 1. Plant leaves 1.1 Grinding and cracking method: 1) Crushing with a grinder: Put the leaves with a diameter of about 5 mm in 50 μL Buffer P1, add steel beads (about 3 mm in diameter, 2 in total) in grinder to break the leaves (45 Hz, 1 min), and the solution with broken leaves will appear green. Centrifuge briefly, keep the supernatant at 4℃ for later use, and take 1 μL for PCR amplification. 2) Mashing with pipette tip: It is recommended to use young leaves. Place leaves with a diameter of about 5 mm in 50 μL Buffer P1, mash the leaves with a pipette tip, the solution turns green after mashing. Centrifuge briefly, keep the supernatant at 4℃ for later use, and take 1 μL for PCR amplification. 1.2 Thermal cracking method: It is recommended to use young leaves. Place leaves with a diameter of about 5 mm in 50 μL Buffer P1 and heat at 95°C for 5-10 min (to ensure that the lysate is completely submerged in the leaves), for leaves that are difficult to lyse (old leaves), the time can be appropriately extended (10-20 min). The solution turns green after heating and lysing. Shake and mix well, centrifuge briefly, keep the supernatant at 4℃ for later use, and take 1 μL for PCR amplification. 1.3 Direct method: It is recommended to use young leaves. Use a hole punch or a knife to directly add leaves with a diameter of about 1 mm into the PCR reaction system; for complex samples or amplification of long fragments, it is recommended to use leaves with a diameter of less than 1 mm. 2. Reaction System Table 1 Amplification system Components Volume (μL) Volume (μL) Final concentration 2× Plant Master Mix 10 25 1× Forward Primer (10 μmol/L) 0.5 1 0.2-0.4 μmol/L Reverse Primer (10 μmol/L) 0.5 1 0.2-0.4 μmol/L Cleavage product (DNA template) 1 2 - ddH2O to 20 to 50 - 【Note】: *All components should be thoroughly mixed before use. a. The amount of template added: less than 5% of the PCR reaction system. Excessive amount will seriously inhibit the PCR reaction. It is strongly recommended to add 1 μL template. For direct amplification of leaves, the grinder cracking method is preferred. b. Final primer concentration: Using 0.2-0.25 μmol/L primer to get better results. When the reaction performance is poor, the primer concentration can be adjusted in the range of 0.1-0.5 μmol/L. c. Reaction system: 20 μL or 50 μL is recommended to ensure the validity and repeatability of target gene amplification. d. System preparation: Prepare the PCR reaction system, place it on a vortexer, vortex and mix, and centrifuge briefly to collect the reaction solution at the bottom of the tube. e. Control reaction: It is recommended to set positive and negative PCR control reactions in order to eliminate the interference of false positives or false negatives when performing PCR. f. In order to store the lysed template more stably, mix the transferred supernatant according to the ratio of lysis product (DNA template): Buffer P2 = 5:1, and store at -20℃ after mixing. Stable preservation varies with time and sample status. If the treated plant leaf supernatant is used for PCR amplification within one week, without adding Buffer P2, the supernatant should be stored at -20℃. 3. Reaction Program             Table 2 Amplification protocol Cycle steps Temperature (℃) Time Cycles Predenaturation 94 5 min 1 Denaturation 94 10 sec 35 Annealing 50-65 20 sec Extension 72 1 min Final extension 72 5 min 1 【Note】： 1) Annealing temperature: Please refer to the theoretical Tm value of the primer. The annealing temperature can be set 2-5℃ lower than the theoretical value of the primer. 2) Extension time: It needs to be determined according to the length of the fragment. For DNA fragments within 1 kb, the recommended extension time is 1 min.  

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IFN gamma, also known as IFNG, is a secreted protein that belongs to the type II interferon family. IFN gamma is produced predominantly by natural killer and natural killer T cells as part of the innate immune response, and by CD4 and CD8 cytotoxic T lymphocyte effector T cells once antigen-specific immunity develops. IFN gamma has antiviral, immunoregulatory, and anti-tumor properties. IFNG, in addition to having antiviral activity, has important immunoregulatory functions, it is a potent activator of macrophages and has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. The IFNG monomer consists of a core of six α-helices and an extended unfolded sequence in the C-terminal region. IFN gamma is critical for innate and adaptive immunity against viral and intracellular bacterial infections and tumor control. Aberrant IFN gamma expression is associated with some autoinflammatory and autoimmune diseases. The importance of IFN gamma in the immune system stems in part from its ability to inhibit viral replication directly, and most importantly from its immunostimulatory and immunomodulatory effects. IFNG also promotes NK cell activity.   Product Properties Synonyms Interferon gamma; IFN-gamma Accession P42161 GeneID 403801 Source E.coli-derived canine Interferon-gamma protein, Gln24-Lys166 Molecular Weight Approximately 16.9 kDa. AA Sequence QAMFFKEIEN LKEYFNASNP DVSDGGSLFV DILKKWREES DKTIIQSQIV SFYLKLFDNF KDNQIIQRSM DTIKEDMLGK FLNSSTSKRE DFLKLIQIPV NDLQVQRKAI NELIKVMNDL SPRSNLRKRK RSQNLFRGRR ASK Tag None Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 97 % by SDS-PAGE and HPLC analyses. Biological Activity Fully biologically active when compared to standard. The ED50 as determined by an anti-viral assay using A-72 canine fibroma cells infected with vesicular stomatitis virus (VSV) is less than 2.0 ng/ml, corresponding to a specific activity of > 5.0 × 105 IU/mg. Endotoxin < 0.1 EU per 1μg of the protein by the LAL method. Formulation Lyophilized from a 0.2 µm filtered concentrated solution in 25 mM Sodium Succinate, pH 5.0, 60 mM NaCl, with 0.1 % Tween-80. Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.   Shipping and Storage The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.   Cautions 1. Avoid repeated freeze-thaw cycles. 2. For your safety and health, please wear lab coats and disposable gloves for operation. 3. For research use only!

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R-Spondin 1 (RSPO1, Roof plate-specific Spondin 1), also known as cysteine-rich and single thrombospondin domain containing protein 3, is a 27 kDa secreted protein that shares ~40% aa identity with three other R-Spondin family members . All R-Spondins regulate Wnt/ beta-Catenin signaling but have distinct expression patterns. In humans, rare disruptions of the R-Spondin 1 gene are associated with tendencies for XX sex reversal (phenotypic male) or hermaphroditism, indicating a role for R-Spondin 1 in gender- Injection of recombinant R-Spondin 1 in mice causes activation of beta-catenin and proliferation of intestinal crypt epithelial cells, and ameliorates experimental colitis. Interest in R-Spondin 1 as a cell culture supplement has grown with the expansion of the organoid field. R-Spondin 1 is widely used in organoid cell culture workflows as a vital component that promotes both growth and survival of 3D organoids . Product Properties Synonyms R-Spondin 1 ；Roof Plate-specific Spondin 1 Accession Q2MKA7 Source CHO Stable Cells-derived human RSPO1 protein, Met1-Ala 263. Molecular Weight Approximately 26.8 kDa. As a result of glycosylation, the apparent molecular mass of it is approximately 40 and 31 kDa in SDS-PAGE under reducing conditions. AA Sequence MRLGLCVVAL VLSWTHLTIS SRGIKGKRQR RISAEGSQAC AKGCELCSEV NGCLKCSPKL FILLERNDIR QVGVCLPSCP PGYFDARNPD MNKCIKCKIE HCEACFSHNF CTKCKEGLYL HKGRCYPACP EGSSAANGTM ECSSPAQCEM SEWSPWGPCS KKQQLCGFRR GSEERTRRVL HAPVGDHAAC SDTKETRRCT VRRVPCPEGQ KRRKGGQGRR ENANRNLARK ESKEAGAGSR RRKGQQQQQQ QGTVGPLTSA GPA     Tag None Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 95% by SDS-PAGE. Biological Activity Measured by its ability to induce activation of ßcatenin response in a Topflash Luciferase assay using HEK293T human embryonic kidney cells. The ED50 for this effect is typically 20-120 ng/mL in the presence of 5 ng/mL recombinant mouse wnt3a. Endotoxin < 1.0 EU per 1μg of the protein by the LAL method. Formulation Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions. Shipping and Storage The products are shipped with ice pack and can be stored at -20℃ for 1 year. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles. Cautions 1.Avoid repeated freeze-thaw cycles. 2.For your safety and health, please wear lab coats and disposable gloves for operation. 3.For research use only.  

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FGF basic is a member of the FGF family, currently comprised of seven related mitogenic proteins which show 35 - 55% amino acid conservation. FGF basic has been isolated from a number of sources, including neural tissue, pituitary, adrenal cortex, corpus luteum and placenta. This factor contains four cysteine residues but reduced FGF basic retains full biological activity, indicating that disulfide bonds are not required for this activity. Several reports indicate that a variety of forms of FGF basic are produced as a result of N-terminal extensions. These extensions apparently affect localization of FGF basic in cellular compartments but do not affect biological activity. Studies indicate that binding of FGF to heparin or cell surface heparan sulfate proteoglycans is necessary for binding of FGF to high affinity FGF receptors. FGF acidic and basic appear to bind to the same high affinity receptors and show a similar range of biological activities. FGF basic stimulates the proliferation of all cells of mesodermal origin, and many cells of neuroectodermal, ectodermal and endodermal origin. FGF basic is chemotactic and mitogenic for endothelial cells in vitro. FGF basic induces neuron differentiation, survival and regeneration. FGF basic has also been shown to be crucial in modulating embryonic development and differentiation. These observed in vitro functions of FGF basic suggest FGF basic may play a role in vivo in the modulation of such normal processes as angiogenesis, wound healing and tissue repair, embryonic development and differentiation, and neuronal function and neural degeneration. Product Properties Synonyms FGF-2, HBGF-2 Accession P03969 GeneID 281161 Source E.coli-derived Bovine bFGF, Pro10-Ser155, with an N-terminal Met. Molecular Weight Approximately 16.5 kDa. AA Sequence MPALPEDGGS GAFPPGHFKD PKRLYCKNGG FFLRIHPDGR VDGVREKSDP HIKLQLQAEE RGVVSIKGVC ANRYLAMKED GRLLASKCVT DECFFFERLE SNNYNTYRSR KYSSWYVALK RTGQYKLGPK TGPGQKAILF LPMSAKS Tag None Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 97% by SDS-PAGE and HPLC analyses. Biological Activity The ED50 as determined by a cell proliferation assay using murine balb/c 3T3 cells is less than 0.1 ng/mL, corresponding to a specific activity of > 1.0 × 107 IU/mg. Fully biologically active when compared to standard. Endotoxin < 1.0 EU per 1μg of the protein by the LAL method. Formulation Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4. Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions Shipping and Storage The products are shipped with ice pack and can be stored at -20 ℃ for 1 year. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 °C under sterile conditions after reconstitution. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles. Cautions Avoid repeated freeze-thaw cycles. For your safety and health, please wear lab coats and disposable gloves for operation. For research use only!

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GCP-2 also known as CXCL6, is a CXC chemokine initially isolated as a neutrophil chemoattractant from the MG-63 osteosarcoma cell line. Among human CXC chemokines, GCP-2 is most closely related to ENA-78 (78% amino acid (aa) sequence identity in the mature peptide region and 86% identity in the signal sequence). The structure and sequence of the genes for human GCP-2 and ENA-78 also exhibit close similarity suggesting the two genes may have originated from a gene duplication. LIX (LPS-induced CXC chemokine) was initially cloned as a gene induced by LPS in mouse fibroblasts. The predicted LIX protein sequence is identical to a previously purified mouse protein designated mouse GCP-2 based on its amino sequence similarity (60% sequence identity) to human GCP-2. Mouse GCP-2/LIX is also 54% identical with human ENA-78 at the amino acid sequence level. Product Properties Synonyms Chemokine alpha 3, CXCL6, GCP-2, CXCL6, member b Accession P80221 GeneID 281735 Source E.coli-derived bovine GCP-2/CXCL6 protein, Gly37-Asn112 Molecular Weight Approximately 8.0 kDa. AA Sequence GPVAAVVREL RCVCLTTTPG IHPKTVSDLQ VIAAGPQCSK VEVIATLKNG REVCLDPEAP LIKKIVQKIL DSGKNN Tag None Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity >97% by SDS-PAGE and HPLC analyses Biological Activity The biological activity determined by a chemotaxis bioassay using human neutrophils is in a concentration range of 10-50 ng/mL. Fully biologically active when compared to standard. Endotoxin < 0.1 EU per 1μg of the protein by the LAL method. Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, 500 mM NaCl, pH 7.0 Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions. Shipping and Storage The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles. Cautions 1. Avoid repeated freeze-thaw cycles. 2. For your safety and health, please wear lab coats and disposable gloves for operation. 3. For research use only!

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Chemokine CXCL9 is a member of the CXC family and has an important role in the chemotaxis of immune cells. The mouse CXCL9 shares 75% and 88% a.a. sequence identity with human and rat CXCL9. Accumulated experimental evidence supports that monokine induced by interferon (IFN)-gamma (CXCL9), a member of CXC chemokine family and known to attract CXCR3- (A and B) T lymphocytes, is involved in the pathogenesis of physiologic diseases during their initiation and their maintenance. It is a cytokine that affects the growth, movement, or activation state of cells that participate in immune and inflammatory response and chemotactic for activated T-cells. Product Properties Synonyms C-X-C motif chemokine 9, CXCL9, Gamma-interferon-induced Monokine, Humig, MIG, Small-inducible Cytokine B9 Accession A9QWP9 GeneID 513990 Source E.coli-derived bovine MIG/CXCL9 protein, Val22-Thr125 . Molecular Weight Approximately 11.9 kDa. AA Sequence VPAIRNGRCS CINTSQGMIH PKSLKDLKQF APSPSCEKTE IIATMKNGNE ACLNPDLPEV KELIKEWEKQ VNQKKKQRKG KKYKKTKKVP KVKRSQRPSQ KKTT Tag None Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity >96% by SDS-PAGE and HPLC analyses. Biological Activity The biological activity determined by a chemotaxis bioassay using human lymphocytes is in a concentration range of 0.1-1.0 ng/mL. Fully biologically active when compared to standard. Endotoxin < 0.1 EU per 1μg of the protein by the LAL method. Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.0, 500 mM NaCl. Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions. Shipping and Storage The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles. Cautions 1. Avoid repeated freeze-thaw cycles. 2. For your safety and health, please wear lab coats and disposable gloves for operation. 3. For research use only!

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CXCL4, also called PF4, is a small cytokine belonging to the CXC chemokine family and it is also known as chemokine (C-X-C motif) ligand. Mature mouse CXCL4 shares 76%, 88%, 64%, 64% and 63% amino acid sequence identity with human, rat, ovine, porcine and bovine CXCL4, respectively. Recombinant mouse CXCL4 contains 76 amino acids which is a single non-glycosylated polypeptide chain. CXCL4 can be antiproliferative and antiangiogenic, at least in part via interfering with FGF-2 and VEGF heparin binding and thus inhibiting their signaling. Tumor tissue revealed up-regulation of CXCL14 in cancer-associated fibroblasts of a majority of prostate cancer. Fibroblasts overexpressing CXCL14 promoted the growth of prostate cancer xenografts, and increased tumor angiogenesis and macrophage infiltration. Product Properties Synonyms chemokine (C-X-C motif) ligand 4, C-X-C motif chemokine 4, CXCL4, CXCL4iroplact, Iroplact, MGC138298, Oncostatin-A, PF4, platelet factor 4 Accession P02777 Unigene Bt.11581. Source E.coli-derived bovine PF-4/CXCL4 protein, Glu1-Ser88. Molecular Weight Approximately 9.5 kDa. AA Sequence ESSFPATFVP LPADSEGGED EDLQCVCLKT TSGINPRHIS SLEVIGAGTH CPSPQLLATK KTGRKICLDQ QRPLYKKILK KLLDGDES Tag None Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity >95% by SDS-PAGE and HPLC analyses. Biological Activity The biological activity determined by a chemotaxis bioassay using human neutrophils is in a concentration of 10-100ng/ml. Fully biologically active when compared to standard. Endotoxin < 0.1 EU per 1μg of the protein by the LAL method. Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, 500 mM NaCl, pH 7.0. Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions. Shipping and Storage The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles. Cautions 1. Avoid repeated freeze-thaw cycles. 2. For your safety and health, please wear lab coats and disposable gloves for operation. 3. For research use only!

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GM-CSF is a hematopoietic growth factor that stimulates the development of neutrophils and macrophages, and promotes the proliferation and development of early erythroid megakaryocytic and eosinophilic progenitor cells. It is produced in endothelial cells, monocytes, fibroblasts and T-lymphocytes. GM-CSF inhibits neutrophil migration and enhances the functional activity of the mature end-cells. The human and murine molecules are species-specific and exhibit no cross-species reactivity. Recombinant Canine GM-CSF is a 14.5 kDa globular protein consisting of 127 amino acids, containing two intramolecular disulfide bonds and two potential N-linked glycosylation sites. Product Properties Synonyms Granulocyte/Macrophage Colony-Stimulating Factor, CSF-2, MGI-1GM, Pluripoietin-α Accession P48749 GeneID 403923 Source E.coli-derived Canine GM-CSF protein,Ala18-Glu144. Molecular Weight Approximately 14.5 kDa. AA Sequence APTRSPTLVT RPSQHVDAIQ EALSLLNNSN DVTAVMNKAV KVVSEVFDPE GPTCLETRLQ LYKEGLQGSL TSLKNPLTMM ANHYKQHCPP TPESPCATQN INFKSFKENL KDFLFNIPFD CWKPVKK Tag None Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 95% by SDS-PAGE and HPLC analyses. Biological Activity Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 5 ng/mL, corresponding to a specific activity of > 2.0 × 10 5 IU/mg. Endotoxin < 1 EU/μg of protein as determined by LAL method. Formulation Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. Shipping and Storage The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles. Cautions 1. Avoid repeated freeze-thaw cycles. 2. For your safety and health, please wear lab coats and disposable gloves for operation. 3. For research use only.

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Interleukin-3 is a pleiotrophic factor produced primarily by activated T cells that can stimulate the proliferation and differentiation of pluripotent hematopoietic stem cells as well as various lineage committed progenitors. IL-3 exerts its biological activities via a heterodimeric receptor composed of an IL-3 specific alpha chain and common beta chain that is shared with the IL-5 and GM-CSF high-affinity receptors. Receptors for IL-3 are present on bone marrow progenitors, and several mature myeloid cell types. Mature canine IL-3 shares <40% amino acid sequence identity with IL-3 of other mammals. Product Properties Synonyms Hematopoietic Growth Factor, MCGF, Multipotential Colony-stimulating Factor, P-cell-stimulating Factor Accession Q9BDX4 GeneID 481497 Source E.coli-derived Canine IL-3, Arg24-Pro143. Molecular Weight Approximately 14.0 kDa. AA Sequence RPFSTDLPKQ YFTMINEIME MLNKSPSPSE EPLDSNEKET LLEDTLLRPN LDVFLNASSK FHKNGLLIWN NLKEFLPLLP TPTPRGEPIS IMENNWGDFQ RKLKKYLEAL DNFLNFKNKP Tag None Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 97% by SDS-PAGE and HPLC analyses. Biological Activity The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 0.2 ng/mL, corresponding to a specific activity of > 5.0 × 10 6 IU/mg. Fully biologically active when compared to standard. Endotoxin < 1.0 EU per 1μg of the protein by the LAL method. Formulation Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4 Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions. Shipping and Storage The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles. Cautions 1. Avoid repeated freeze-thaw cycles. 2. For your safety and health, please wear lab coats and disposable gloves for operation. 3. For research use only!

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Interleukin-8 (IL-8), also known as CXCL8, GCP-1, and NAP-1, is a widely expressed proinflammatory member of the CXC family of chemokines. Near its N-terminus, this 8-9 kDa chemokine contains an ELR motif which is important for its angiogenic properties. CXCL8 can associate into a homodimer or a heterodimer with CXCL4/PF4, and it can also interact with matrix and cell surface glycosaminoglycans. Mature canine CXCL8 shares 87%, 69%, and 82% amino acid (aa) sequence identiity with feline, human, and porcine CXCL8. There is no CXCL8 gene counterpart in rodent. N-terminal truncation of CXCL8 by multiple proteases generates a range of shorter forms. The bioactivity of CXCL8 is regulated by these truncations, by CXCL8 citrullination at Arg5 (N-terminal to  the ELR motif), and by the decoy receptor DARC. CXCL8 effects are mediated through CXCR1/IL-8 RA, which is also used by CXCL6, and through CXCR2/IL-8 RB, which is used by multiple CXC chemokines. These receptors associate into functional homodimers and heterodimers with each other. Through both CXCR1 and CXCR2, CXCL8 promotes neutrophil adhesion to the vascular endothelium and migration to sites of inflammation. It triggers the antimicrobial activation of neutrophils through CXCR1. CXCL8 also binds to Serpin A1/alpha-1 Antitrypsin, and this prevents CXCL8 interaction with CXCR1. CXCL8 is upregulated in atherosclerotic lesions and other cardiac pathologies where it exacerbates inflammatory tissue damage. In addition, it induces VEGF expression, vascular endothelial cell proliferation, angiogenesis, and tumor cell invasiveness. Product Properties Synonyms (Ser-IL-8)72, GCP/IL-8 protein I, IL8/NAP1 form III, LYNAP, MDNCF-c, NAF Accession P41324 GeneID 403850 Source E.coli-derived Canine IL-8/CXCL8, Ala23-Pro101. Molecular Weight Approximately 9.1 kDa. AA Sequence AVLSRVSSEL RCQCIKTHST PFHPKYIKEL RVIDSGPHCE NSEIIVKLFN GNEVCLDPKE KWVQKVVQIF LKKAEKQDP Tag None Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 95% by SDS-PAGE and HPLC analyses. Biological Activity The biological activity determined by a chemotaxis bioassay using human CXCR2 transfected murine BaF3 cells is in a concentration range of 0.15-0.75 ng/mL. Fully biologically active when compared to standard. Endotoxin < 1.0 EU per 1μg of the protein by the LAL method. Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 2 × PBS, pH 7.4. Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions. Shipping and Storage The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles. Cautions 1. Avoid repeated freeze-thaw cycles. 2. For your safety and health, please wear lab coats and disposable gloves for operation. 3. For research use only!

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MCP-2 and MCP-3 are two monocyte chemotactic proteins produced by human MG-63 osteosarcoma cells. Both MCP-2 and MCP-3 are members of the C-C family of chemokines and share 62% and 71% amino acid sequence identity, respectively, with MCP-1. MCP-3 also shares 58% amino acid identity with MCP-2. Product Properties Synonyms CCL8 Accession Q68AY9 GeneID 44879 Source E.coli-derived Canine CCL8 protein,Gln24--Pro99. Molecular Weight Approximately 8.8 kDa AA Sequence QPDSVSIPIT CCFSMVKRKI PMQKLESYMR ITNSQCPQEA VIFKTKASRE ICADPKQKWV QDYMNHLDQK SQAQKP Tag None Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 98% by SDS-PAGE and HPLC analyses. Biological Activity Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 10-100 ng/ml Endotoxin <0.1 EU/μg of protein as determined by LAL method. Formulation Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions. Shipping and Storage The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles. Cautions 1. Avoid repeated freeze-thaw cycles. 2. For your safety and health, please wear lab coats and disposable gloves for operation. 3. For research use only.

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Stem Cell Factor (SCF) which binds to the c-Kit receptor is produced by fibroblasts and endothelial cells. The soluble and transmembrane forms of the protein are formed by alternative splicing of the same RNA transcript and the presence of both soluble and transmembrane SCF is required for normal hematopoietic function. SCF plays an important role in hematopoiesis, spermatogenesis and melanogenesis and it promotes mast cell adhesion, migration, proliferation, and survival. Soluble canine SCF shares 88 %, 93 %, 86 %, 83 %, 76 %, 76 %, 86 % and 88 % a.a. sequence identity with porcine, feline, bovine, human, mouse, rat, goat and equine SCF, respectively. Cells known to express SCF include endothelial cells, fibroblasts and keratinocytes .   Product Properties Synonyms Clo Protein, Con Protein, Gb Protein, Kitlg Protein, Mgf Protein, SCF Protein, SF Protein, Sl Protein, SLF Protein Accession Q06220 GeneID 403507 Source E.coli-derived canine Stem Cell Factor, Lys26-Ala190 Molecular Weight Approximately 18.4 kDa. AA Sequence KGICGKRVTD DVKDVTKLVA NLPKDYKIAL KYVPGMDVLP SHCWISVMVE QLSVSLTDLL DKFSNISEGL SNYSIIDKLV KIVDDLVECT EGYSFENVKK APKSPELRLF TPEEFFRIFN RSIDAFKDLE TVASKSSECV VSSTLSPDKD SRVSVTKPFM LPPVA Tag None Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 96 % by SDS-PAGE and HPLC analyses. Biological Activity Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 2.0 ng/ml, corresponding to a specific activity of > 5.0 × 105 IU/mg. Endotoxin < 1.0 EU per 1μg of the protein by the LAL method. Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 2 × PBS, pH 7.4. Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.   Shipping and Storage The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.   Cautions 1. Avoid repeated freeze-thaw cycles. 2. For your safety and health, please wear lab coats and disposable gloves for operation. 3. For research use only!

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