Mouse TNF-α ELISA Kit_P162006

Description

Tumor necrosis factor-alpha (TNF-α), also known as cachexin or TNFSF1A, is a multifunctional cytokine that plays a central role in inflammation, apoptosis, and immune system development. It is produced by various immune cells, epithelial cells, endothelial cells, and tumor cells. TNF-α exerts its effects through receptors TNFR1 and TNFR2, activating signaling pathways involving caspase 8, transcription factor NF-κB, and kinase JNK.

Human TNF-α shares 79% amino acid homology with mouse TNF-α, suggesting that its biological functions may not exhibit significant species specificity. TNF-α has been shown to confer resistance against certain types of infections while paradoxically inducing inflammation in pathological processes. It may also affect glucose uptake and insulin resistance. Additionally, TNF-α plays a critical role in tumor proliferation, migration, invasion, and angiogenesis.

The Arcegen Mouse TNF-α ELISA assay kit is an in vitro enzyme-linked immunosorbent assay (ELISA) kit designed to quantitatively measure mouse TNF-α in serum and plasma samples. The assay utilizes high-affinity anti-mouse TNF-α antibodies pre-coated onto an ELISA plate. Standard samples and test samples are added to the plate wells and incubated, allowing mouse TNF-α present in the samples to bind to the solid-phase antibodies. After washing to remove unbound substances, a detection antibody is added for incubation, followed by addition of enzyme conjugate (Streptavidin-HRP). After washing again, a colorimetric substrate (TMB) is added for color development, and the intensity of the color reaction is proportional to the concentration of mouse TNF-α in the samples. The reaction is terminated, and absorbance is measured at 450 nm wavelength (with a reference wavelength of 570-630 nm).

Specification

Item Number P162006S / P162006E
Specification 48 T / 96 T
Detection Range 31.25-2000 pg/mL
Detection Method Sandwich ELISA
Species Detected Mouse
Detection Time 4.5 hours
Sensitivity 7.82 pg/mL
Dilution Linearity 84 - 124%
Recovery Rate 81 - 119%
Intra-assay Variability 3.8%
Intra-assay Variability 5.3%

Components

Component Number Component Name Storage Temperature P162006S P162006E
P162006-A ELISA Plate 2~8℃ 48 T 96 T
P162006-B Standard sample 2~8℃ 1 tube 2 tubes
P162006-C Detection antibody 2~8℃ 120 μL 240 μL
P162006-D Enzyme conjugate 2~8℃(Avoid Light) 30 μL 60 μL
P162006-E 5× dilution buffer 2~8℃ 8 mL 15 mL
P162006-F 20× wash buffer 2~8℃ 25 mL 50 mL
P162006-G Substrate solution 2~8℃(Avoid Light) 8 mL 15 mL
P162006-H Stop solution Room Temperature 5 mL 10 mL
P162006-I Plate sealing film Room Temperature 3 pieces 5 pieces

Storage

The kit can be stored at 2~8°C, or according to the storage conditions of individual components to avoid contamination and repeated freeze-thaw cycles. Diluted reagents prepared to working concentration should be used immediately and discarded; they should not be reused. The shelf life is 1 year.

Table 1 Reagent Storage Table After Initial Use

     Component Name Storage Conditions
    ELISA Plate   Unused strips can be returned to the aluminum foil bag, tightly sealed, and stored at 2~8°C to avoid moisture absorption.
    Standard sample Use within 48 hours after dissolution, store at 2~8°C to avoid contamination.
    Detection antibody Use within 48 hours after dilution, store at 2~8°C to avoid contamination.
    Enzyme conjugate Use within 48 hours after dilution, store at 2~8°C to avoid contamination.
    5× dilution buffer Store at 2~8°C for 1 month, avoiding contamination.
    20× wash buffer Store at 2~8°C for 1 month, avoiding contamination.
    Substrate solution Store at 2~8°C for 1 month, avoiding light exposure.
    Stop solution Can be stored at room temperature.
    Plate sealing film Can be stored at room temperature.

                                      

    Documents:

    Mouse TNF-α ELISA Kit_P162006

    Product form

    SKU: P162006E

    $900.00$375.00

        • Tell a unique detail about this product

        Description

        Tumor necrosis factor-alpha (TNF-α), also known as cachexin or TNFSF1A, is a multifunctional cytokine that plays a central role in inflammation, apoptosis, and immune system development. It is produced by various immune cells, epithelial cells, endothelial cells, and tumor cells. TNF-α exerts its effects through receptors TNFR1 and TNFR2, activating signaling pathways involving caspase 8, transcription factor NF-κB, and kinase JNK.

        Human TNF-α shares 79% amino acid homology with mouse TNF-α, suggesting that its biological functions may not exhibit significant species specificity. TNF-α has been shown to confer resistance against certain types of infections while paradoxically inducing inflammation in pathological processes. It may also affect glucose uptake and insulin resistance. Additionally, TNF-α plays a critical role in tumor proliferation, migration, invasion, and angiogenesis.

        The Arcegen Mouse TNF-α ELISA assay kit is an in vitro enzyme-linked immunosorbent assay (ELISA) kit designed to quantitatively measure mouse TNF-α in serum and plasma samples. The assay utilizes high-affinity anti-mouse TNF-α antibodies pre-coated onto an ELISA plate. Standard samples and test samples are added to the plate wells and incubated, allowing mouse TNF-α present in the samples to bind to the solid-phase antibodies. After washing to remove unbound substances, a detection antibody is added for incubation, followed by addition of enzyme conjugate (Streptavidin-HRP). After washing again, a colorimetric substrate (TMB) is added for color development, and the intensity of the color reaction is proportional to the concentration of mouse TNF-α in the samples. The reaction is terminated, and absorbance is measured at 450 nm wavelength (with a reference wavelength of 570-630 nm).

        Specification

        Item Number P162006S / P162006E
        Specification 48 T / 96 T
        Detection Range 31.25-2000 pg/mL
        Detection Method Sandwich ELISA
        Species Detected Mouse
        Detection Time 4.5 hours
        Sensitivity 7.82 pg/mL
        Dilution Linearity 84 - 124%
        Recovery Rate 81 - 119%
        Intra-assay Variability 3.8%
        Intra-assay Variability 5.3%

        Components

        Component Number Component Name Storage Temperature P162006S P162006E
        P162006-A ELISA Plate 2~8℃ 48 T 96 T
        P162006-B Standard sample 2~8℃ 1 tube 2 tubes
        P162006-C Detection antibody 2~8℃ 120 μL 240 μL
        P162006-D Enzyme conjugate 2~8℃(Avoid Light) 30 μL 60 μL
        P162006-E 5× dilution buffer 2~8℃ 8 mL 15 mL
        P162006-F 20× wash buffer 2~8℃ 25 mL 50 mL
        P162006-G Substrate solution 2~8℃(Avoid Light) 8 mL 15 mL
        P162006-H Stop solution Room Temperature 5 mL 10 mL
        P162006-I Plate sealing film Room Temperature 3 pieces 5 pieces

        Storage

        The kit can be stored at 2~8°C, or according to the storage conditions of individual components to avoid contamination and repeated freeze-thaw cycles. Diluted reagents prepared to working concentration should be used immediately and discarded; they should not be reused. The shelf life is 1 year.

        Table 1 Reagent Storage Table After Initial Use

           Component Name Storage Conditions
          ELISA Plate   Unused strips can be returned to the aluminum foil bag, tightly sealed, and stored at 2~8°C to avoid moisture absorption.
          Standard sample Use within 48 hours after dissolution, store at 2~8°C to avoid contamination.
          Detection antibody Use within 48 hours after dilution, store at 2~8°C to avoid contamination.
          Enzyme conjugate Use within 48 hours after dilution, store at 2~8°C to avoid contamination.
          5× dilution buffer Store at 2~8°C for 1 month, avoiding contamination.
          20× wash buffer Store at 2~8°C for 1 month, avoiding contamination.
          Substrate solution Store at 2~8°C for 1 month, avoiding light exposure.
          Stop solution Can be stored at room temperature.
          Plate sealing film Can be stored at room temperature.

                                            

          Documents:

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