Description
R-Spondin 1 (RSPO1, Roof plate-specific Spondin 1), also known as cysteine-rich and single thrombospondin domain containing protein 3, is a 27 kDa secreted protein that shares ~40% aa identity with three other R-Spondin family members . All R-Spondins regulate Wnt/ beta-Catenin signaling but have distinct expression patterns. In humans, rare disruptions of the R-Spondin 1 gene are associated with tendencies for XX sex reversal (phenotypic male) or hermaphroditism, indicating a role for R-Spondin 1 in gender- Injection of recombinant R-Spondin 1 in mice causes activation of beta-catenin and proliferation of intestinal crypt epithelial cells, and ameliorates experimental colitis. Interest in R-Spondin 1 as a cell culture supplement has grown with the expansion of the organoid field. R-Spondin 1 is widely used in organoid cell culture workflows as a vital component that promotes both growth and survival of 3D organoids .
Product Properties
|
Synonyms
|
R-Spondin 1 ；Roof Plate-specific Spondin 1
|
|
Accession
|
Q2MKA7
|
|
Source
|
CHO Stable Cells-derived human RSPO1 protein, Met1-Ala 263.
|
|
Molecular Weight
|
Approximately 26.8 kDa. As a result of glycosylation, the apparent molecular mass of it is approximately 40 and 31 kDa in SDS-PAGE under reducing conditions.
|
|
AA Sequence
|
MRLGLCVVAL VLSWTHLTIS SRGIKGKRQR RISAEGSQAC AKGCELCSEV NGCLKCSPKL FILLERNDIR QVGVCLPSCP PGYFDARNPD MNKCIKCKIE HCEACFSHNF CTKCKEGLYL HKGRCYPACP EGSSAANGTM ECSSPAQCEM SEWSPWGPCS KKQQLCGFRR GSEERTRRVL HAPVGDHAAC SDTKETRRCT VRRVPCPEGQ KRRKGGQGRR ENANRNLARK ESKEAGAGSR RRKGQQQQQQ QGTVGPLTSA GPA
|
|
Tag
|
None
|
|
Physical Appearance
|
Sterile Filtered White lyophilized (freeze-dried) powder.
|
|
Purity
|
> 95% by SDS-PAGE.
|
|
Biological Activity
|
Measured by its ability to induce activation of ßcatenin response in a Topflash Luciferase assay using HEK293T human embryonic kidney cells. The ED50 for this effect is typically 20-120 ng/mL in the presence of 5 ng/mL recombinant mouse wnt3a.
|
|
Endotoxin
|
< 1.0 EU per 1μg of the protein by the LAL method.
|
|
Formulation
|
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
|
|
Reconstitution
|
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions.
|
Shipping and Storage
The products are shipped with ice pack and can be stored at -20℃ for 1 year.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
Cautions
1.Avoid repeated freeze-thaw cycles.
2.For your safety and health, please wear lab coats and disposable gloves for operation.
3.For research use only.
