Recombinant Canine Granulocyte-Macrophage Colony Stimulating Factor (Canine GM-CSF)_C230267

Description

GM-CSF is a hematopoietic growth factor that stimulates the development of neutrophils and macrophages, and promotes the proliferation and development of early erythroid megakaryocytic and eosinophilic progenitor cells. It is produced in endothelial cells, monocytes, fibroblasts and T-lymphocytes. GM-CSF inhibits neutrophil migration and enhances the functional activity of the mature end-cells. The human and murine molecules are species-specific and exhibit no cross-species reactivity. Recombinant Canine GM-CSF is a 14.5 kDa globular protein consisting of 127 amino acids, containing two intramolecular disulfide bonds and two potential N-linked glycosylation sites.

Product Properties

Synonyms
Granulocyte/Macrophage Colony-Stimulating Factor, CSF-2, MGI-1GM, Pluripoietin-α
Accession
P48749
GeneID
403923
Source
E.coli-derived Canine GM-CSF protein,Ala18-Glu144.
Molecular Weight
Approximately 14.5 kDa.
AA Sequence
APTRSPTLVT RPSQHVDAIQ EALSLLNNSN DVTAVMNKAV KVVSEVFDPE GPTCLETRLQ LYKEGLQGSL TSLKNPLTMM ANHYKQHCPP TPESPCATQN INFKSFKENL KDFLFNIPFD CWKPVKK
Tag
None
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
> 95% by SDS-PAGE and HPLC analyses.
Biological Activity
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 5 ng/mL, corresponding to a specific activity of > 2.0 × 10 5 IU/mg.
Endotoxin
< 1 EU/μg of protein as determined by LAL method.
Formulation
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of
0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.



Shipping and Storage

The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

Cautions

1. Avoid repeated freeze-thaw cycles.
2. For your safety and health, please wear lab coats and disposable gloves for operation.
3. For research use only.

Recombinant Canine Granulocyte-Macrophage Colony Stimulating Factor (Canine GM-CSF)_C230267

Product form

SKU: C230267E

$82.00

    • Shipped today? Order within: Oct 04, 2024 17:00:00 -0400
    • Tell a unique detail about this product

    Description

    GM-CSF is a hematopoietic growth factor that stimulates the development of neutrophils and macrophages, and promotes the proliferation and development of early erythroid megakaryocytic and eosinophilic progenitor cells. It is produced in endothelial cells, monocytes, fibroblasts and T-lymphocytes. GM-CSF inhibits neutrophil migration and enhances the functional activity of the mature end-cells. The human and murine molecules are species-specific and exhibit no cross-species reactivity. Recombinant Canine GM-CSF is a 14.5 kDa globular protein consisting of 127 amino acids, containing two intramolecular disulfide bonds and two potential N-linked glycosylation sites.

    Product Properties

    Synonyms
    Granulocyte/Macrophage Colony-Stimulating Factor, CSF-2, MGI-1GM, Pluripoietin-α
    Accession
    P48749
    GeneID
    403923
    Source
    E.coli-derived Canine GM-CSF protein,Ala18-Glu144.
    Molecular Weight
    Approximately 14.5 kDa.
    AA Sequence
    APTRSPTLVT RPSQHVDAIQ EALSLLNNSN DVTAVMNKAV KVVSEVFDPE GPTCLETRLQ LYKEGLQGSL TSLKNPLTMM ANHYKQHCPP TPESPCATQN INFKSFKENL KDFLFNIPFD CWKPVKK
    Tag
    None
    Physical Appearance
    Sterile Filtered White lyophilized (freeze-dried) powder.
    Purity
    > 95% by SDS-PAGE and HPLC analyses.
    Biological Activity
    Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 5 ng/mL, corresponding to a specific activity of > 2.0 × 10 5 IU/mg.
    Endotoxin
    < 1 EU/μg of protein as determined by LAL method.
    Formulation
    Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
    Reconstitution

    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of
    0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.



    Shipping and Storage

    The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.
    Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

    Cautions

    1. Avoid repeated freeze-thaw cycles.
    2. For your safety and health, please wear lab coats and disposable gloves for operation.
    3. For research use only.

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