Mouse IL-20 ELISA Kit_P162017

Description

Mouse IL-20 ELISA (Enzyme-Linked Immunosorbent Assay) Kit is an in vitro enzyme-linked immunosorbent assay kit used for quantitative determination of IL-20 in serum, plasma, and cell culture supernatants. Specific anti-IL-20 antibodies are pre-coated onto high-affinity enzyme plates. Standards and samples are added to the wells of the enzyme plate, and after incubation, IL-20 present in the sample binds to the solid-phase antibody. After washing to remove unbound substances, a detection antibody is added for binding and incubated, followed by washing, and then the enzyme complex (Streptavidin-HRP) is added for binding and incubated. After washing, a colorimetric substrateTMB is added for color development, avoiding light. The intensity of the color reaction is proportional to the concentration of IL-20 in the sample. The reaction is terminated by adding a stop solution, and the absorbance is measured at 450 nm wavelength (with a reference wavelength of 570 - 630 nm).

IL-20 belongs to the TGF-β superfamily. IL-20 binds two heterodimeric receptor complexes, IL-20 R alpha/IL-20 R beta and IL-22 R/IL-20 R beta. IL-20 is reported to enhances tissue remodeling and wound-healing activities and restores the homeostasis of epithelial layers during infection and inflammatory responses to maintain tissue integrity.

Specification

Item Number P162017S / P162017E
Specification 48 T / 96 T
Detection Range 31.25-2000 pg/mL
Detection Method Sandwich ELISA
Detection Species Mouse
Detection Time 4.5 hours
Sensitivity 8.22 pg/mL
Dilution Linearity 91 - 120%
Recovery Rate 91 - 115%
Intra-assay Variability 3.7%
Intra-assay Variability 6.1%

Components

Component Number Component Name Storage Temperature P162017S P162017E
P162017-A ELISA Plate 2~8℃ 48 T 96 T
P162017-B Standard 2~8℃ 1 tube 2 tubes
P162017-C Detection Antibody 2~8℃ 60 μL 120 μL
P162017-D Enzyme Conjugate 2~8℃(Avoid Light) 30 μL 60 μL
P162017-E 5x Dilution Buffer 2~8℃ 8 mL 15 mL
P162017-F 20x Wash Buffer 2~8℃ 25 mL 50 mL
P162017-G

Substrate Solution

2~8℃(Avoid Light) 8 mL 15 mL
P162017-H Stop Solution Room Temperature 5 mL 10 mL
P162017-I Plate Sealers Room Temperature 3 pieces 5 pieces

Storage

The assay kit can be stored at 2~8℃. Alternatively, the reagents can be stored according to the storage conditions provided in the component information to avoid contamination and repeated freeze-thaw cycles. Diluted working solutions should be used immediately and not reused. The shelf life is 1 year.

Table 1 Reagent Storage Table After First Use

     Material Name Storage Conditions
    Plate   Unused strips can be returned to the aluminum foil bag, tightly sealed, and stored at 2~8°C to avoid moisture absorption.
    Standard Use within 48 hours after dissolution, store at 2~8°C to avoid contamination.
    Detection Antibody Use within 48 hours after dilution, store at 2~8°C to avoid contamination.
    Enzyme conjugate Use within 48 hours after dilution, store at 2~8°C to avoid contamination.
    5×Dilution Buffer Store at 2~8°C for 1 month, avoiding contamination.
    20×Wash Buffer Store at 2~8°C for 1 month, avoiding contamination.
    Substrate solution Store at 2~8°C for 1 month, avoiding light exposure.
    Stop Solution Can be stored at room temperature.
    Plate Sealers Can be stored at room temperature.

                                      

    Documents:

    Mouse IL-20 ELISA Kit_P162017

    Product form

    SKU: P162017E

    $900.00$375.00

      • Shipped today? Order within: Jul 17, 2024 17:00:00 -0400
      • Tell a unique detail about this product

      Description

      Mouse IL-20 ELISA (Enzyme-Linked Immunosorbent Assay) Kit is an in vitro enzyme-linked immunosorbent assay kit used for quantitative determination of IL-20 in serum, plasma, and cell culture supernatants. Specific anti-IL-20 antibodies are pre-coated onto high-affinity enzyme plates. Standards and samples are added to the wells of the enzyme plate, and after incubation, IL-20 present in the sample binds to the solid-phase antibody. After washing to remove unbound substances, a detection antibody is added for binding and incubated, followed by washing, and then the enzyme complex (Streptavidin-HRP) is added for binding and incubated. After washing, a colorimetric substrateTMB is added for color development, avoiding light. The intensity of the color reaction is proportional to the concentration of IL-20 in the sample. The reaction is terminated by adding a stop solution, and the absorbance is measured at 450 nm wavelength (with a reference wavelength of 570 - 630 nm).

      IL-20 belongs to the TGF-β superfamily. IL-20 binds two heterodimeric receptor complexes, IL-20 R alpha/IL-20 R beta and IL-22 R/IL-20 R beta. IL-20 is reported to enhances tissue remodeling and wound-healing activities and restores the homeostasis of epithelial layers during infection and inflammatory responses to maintain tissue integrity.

      Specification

      Item Number P162017S / P162017E
      Specification 48 T / 96 T
      Detection Range 31.25-2000 pg/mL
      Detection Method Sandwich ELISA
      Detection Species Mouse
      Detection Time 4.5 hours
      Sensitivity 8.22 pg/mL
      Dilution Linearity 91 - 120%
      Recovery Rate 91 - 115%
      Intra-assay Variability 3.7%
      Intra-assay Variability 6.1%

      Components

      Component Number Component Name Storage Temperature P162017S P162017E
      P162017-A ELISA Plate 2~8℃ 48 T 96 T
      P162017-B Standard 2~8℃ 1 tube 2 tubes
      P162017-C Detection Antibody 2~8℃ 60 μL 120 μL
      P162017-D Enzyme Conjugate 2~8℃(Avoid Light) 30 μL 60 μL
      P162017-E 5x Dilution Buffer 2~8℃ 8 mL 15 mL
      P162017-F 20x Wash Buffer 2~8℃ 25 mL 50 mL
      P162017-G

      Substrate Solution

      2~8℃(Avoid Light) 8 mL 15 mL
      P162017-H Stop Solution Room Temperature 5 mL 10 mL
      P162017-I Plate Sealers Room Temperature 3 pieces 5 pieces

      Storage

      The assay kit can be stored at 2~8℃. Alternatively, the reagents can be stored according to the storage conditions provided in the component information to avoid contamination and repeated freeze-thaw cycles. Diluted working solutions should be used immediately and not reused. The shelf life is 1 year.

      Table 1 Reagent Storage Table After First Use

         Material Name Storage Conditions
        Plate   Unused strips can be returned to the aluminum foil bag, tightly sealed, and stored at 2~8°C to avoid moisture absorption.
        Standard Use within 48 hours after dissolution, store at 2~8°C to avoid contamination.
        Detection Antibody Use within 48 hours after dilution, store at 2~8°C to avoid contamination.
        Enzyme conjugate Use within 48 hours after dilution, store at 2~8°C to avoid contamination.
        5×Dilution Buffer Store at 2~8°C for 1 month, avoiding contamination.
        20×Wash Buffer Store at 2~8°C for 1 month, avoiding contamination.
        Substrate solution Store at 2~8°C for 1 month, avoiding light exposure.
        Stop Solution Can be stored at room temperature.
        Plate Sealers Can be stored at room temperature.

                                          

        Documents:

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