Mouse IL-20 ELISA (Enzyme-Linked Immunosorbent Assay) Kit is an in vitro enzyme-linked immunosorbent assay kit used for quantitative determination of IL-20 in serum, plasma, and cell culture supernatants. Specific anti-IL-20 antibodies are pre-coated onto high-affinity enzyme plates. Standards and samples are added to the wells of the enzyme plate, and after incubation, IL-20 present in the sample binds to the solid-phase antibody. After washing to remove unbound substances, a detection antibody is added for binding and incubated, followed by washing, and then the enzyme complex (Streptavidin-HRP) is added for binding and incubated. After washing, a colorimetric substrateTMB is added for color development, avoiding light. The intensity of the color reaction is proportional to the concentration of IL-20 in the sample. The reaction is terminated by adding a stop solution, and the absorbance is measured at 450 nm wavelength (with a reference wavelength of 570 - 630 nm).
IL-20 belongs to the TGF-β superfamily. IL-20 binds two heterodimeric receptor complexes, IL-20 R alpha/IL-20 R beta and IL-22 R/IL-20 R beta. IL-20 is reported to enhances tissue remodeling and wound-healing activities and restores the homeostasis of epithelial layers during infection and inflammatory responses to maintain tissue integrity.
Specification
|
Item Number
|
P162017S / P162017E |
| Specification |
48 T / 96 T |
| Detection Range |
31.25-2000 pg/mL |
| Detection Method |
Sandwich ELISA |
| Detection Species |
Mouse |
| Detection Time |
4.5 hours |
| Sensitivity |
8.22 pg/mL |
| Dilution Linearity |
91 - 120% |
| Recovery Rate |
91 - 115% |
| Intra-assay Variability |
3.7% |
| Intra-assay Variability |
6.1% |
Components
| Component Number |
Component Name |
Storage Temperature |
P162017S |
P162017E |
| P162017-A |
ELISA Plate |
2~8℃ |
48 T |
96 T |
| P162017-B |
Standard |
2~8℃ |
1 tube |
2 tubes |
| P162017-C |
Detection Antibody |
2~8℃ |
60 μL |
120 μL |
| P162017-D |
Enzyme Conjugate |
2~8℃(Avoid Light) |
30 μL |
60 μL |
| P162017-E |
5x Dilution Buffer |
2~8℃ |
8 mL |
15 mL |
| P162017-F |
20x Wash Buffer |
2~8℃ |
25 mL |
50 mL |
| P162017-G |
Substrate Solution
|
2~8℃(Avoid Light) |
8 mL |
15 mL |
| P162017-H |
Stop Solution |
Room Temperature |
5 mL |
10 mL |
| P162017-I |
Plate Sealers |
Room Temperature |
3 pieces |
5 pieces |
Storage
The assay kit can be stored at 2~8℃. Alternatively, the reagents can be stored according to the storage conditions provided in the component information to avoid contamination and repeated freeze-thaw cycles. Diluted working solutions should be used immediately and not reused. The shelf life is 1 year.
Table 1 Reagent Storage Table After First Use
| Material Name
|
Storage Conditions |
| Plate |
Unused strips can be returned to the aluminum foil bag, tightly sealed, and stored at 2~8°C to avoid moisture absorption. |
| Standard |
Use within 48 hours after dissolution, store at 2~8°C to avoid contamination.
|
| Detection Antibody |
Use within 48 hours after dilution, store at 2~8°C to avoid contamination. |
| Enzyme conjugate |
Use within 48 hours after dilution, store at 2~8°C to avoid contamination. |
| 5×Dilution Buffer |
Store at 2~8°C for 1 month, avoiding contamination. |
| 20×Wash Buffer |
Store at 2~8°C for 1 month, avoiding contamination. |
| Substrate solution |
Store at 2~8°C for 1 month, avoiding light exposure. |
| Stop Solution |
Can be stored at room temperature. |
| Plate Sealers |
Can be stored at room temperature. |
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