Recombinant Canine Interleukin-3 (Canine IL-3)_C230269

Description

Interleukin-3 is a pleiotrophic factor produced primarily by activated T cells that can stimulate the proliferation and differentiation of pluripotent hematopoietic stem cells as well as various lineage committed progenitors. IL-3 exerts its biological activities via a
heterodimeric receptor composed of an IL-3 specific alpha chain and common beta chain that is shared with the IL-5 and GM-CSF high-affinity receptors. Receptors for IL-3 are present on bone marrow progenitors, and several mature myeloid cell types. Mature
canine IL-3 shares <40% amino acid sequence identity with IL-3 of other mammals.

Product Properties

Synonyms

Hematopoietic Growth Factor, MCGF, Multipotential Colony-stimulating Factor, P-cell-stimulating Factor
Accession
Q9BDX4
GeneID
481497
Source
E.coli-derived Canine IL-3, Arg24-Pro143.
Molecular Weight

Approximately 14.0 kDa.
AA Sequence
RPFSTDLPKQ YFTMINEIME MLNKSPSPSE EPLDSNEKET LLEDTLLRPN LDVFLNASSK FHKNGLLIWN NLKEFLPLLP TPTPRGEPIS IMENNWGDFQ RKLKKYLEAL DNFLNFKNKP
Tag
None
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
> 97% by SDS-PAGE and HPLC analyses.
Biological Activity
The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 0.2 ng/mL, corresponding to a specific activity of > 5.0 × 10 6 IU/mg. Fully biologically active when compared to
standard.
Endotoxin
< 1.0 EU per 1μg of the protein by the LAL method.
Formulation
Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4
Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions.



Shipping and Storage

The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

Cautions

1. Avoid repeated freeze-thaw cycles.
2. For your safety and health, please wear lab coats and disposable gloves for operation.
3. For research use only!

Recombinant Canine Interleukin-3 (Canine IL-3)_C230269

Product form

SKU: C230269E

$155.00

      • Tell a unique detail about this product

      Description

      Interleukin-3 is a pleiotrophic factor produced primarily by activated T cells that can stimulate the proliferation and differentiation of pluripotent hematopoietic stem cells as well as various lineage committed progenitors. IL-3 exerts its biological activities via a
      heterodimeric receptor composed of an IL-3 specific alpha chain and common beta chain that is shared with the IL-5 and GM-CSF high-affinity receptors. Receptors for IL-3 are present on bone marrow progenitors, and several mature myeloid cell types. Mature
      canine IL-3 shares <40% amino acid sequence identity with IL-3 of other mammals.

      Product Properties

      Synonyms

      Hematopoietic Growth Factor, MCGF, Multipotential Colony-stimulating Factor, P-cell-stimulating Factor
      Accession
      Q9BDX4
      GeneID
      481497
      Source
      E.coli-derived Canine IL-3, Arg24-Pro143.
      Molecular Weight

      Approximately 14.0 kDa.
      AA Sequence
      RPFSTDLPKQ YFTMINEIME MLNKSPSPSE EPLDSNEKET LLEDTLLRPN LDVFLNASSK FHKNGLLIWN NLKEFLPLLP TPTPRGEPIS IMENNWGDFQ RKLKKYLEAL DNFLNFKNKP
      Tag
      None
      Physical Appearance
      Sterile Filtered White lyophilized (freeze-dried) powder.
      Purity
      > 97% by SDS-PAGE and HPLC analyses.
      Biological Activity
      The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 0.2 ng/mL, corresponding to a specific activity of > 5.0 × 10 6 IU/mg. Fully biologically active when compared to
      standard.
      Endotoxin
      < 1.0 EU per 1μg of the protein by the LAL method.
      Formulation
      Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4
      Reconstitution

      We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions.



      Shipping and Storage

      The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.
      Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

      Cautions

      1. Avoid repeated freeze-thaw cycles.
      2. For your safety and health, please wear lab coats and disposable gloves for operation.
      3. For research use only!

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