Mouse IL-12 p70 ELISA Kit_P162016

Description

Mouse IL-12p70 ELISA (Enzyme-Linked Immunosorbent Assay) Kit is an in vitro enzyme-linked immunosorbent assay kit used for quantitative determination of IL-12p70 in serum, plasma, and cell culture supernatants. Specific anti-IL-12p70 antibodies are pre-coated onto high-affinity enzyme plates. Standards and samples are added to the wells of the enzyme plate, and after incubation, IL-12p70 present in the sample binds to the solid-phase antibody. After washing to remove unbound substances, a detection antibody is added for binding and incubated, followed by washing, and then the enzyme complex (Streptavidin-HRP) is added for binding and incubated. After washing, a colorimetric substrateTMB is added for color development, avoiding light. The intensity of the color reaction is proportional to the concentration of IL-12p70 in the sample. The reaction is terminated by adding a stop solution, and the absorbance is measured at 450 nm wavelength (with a reference wavelength of 570 - 630 nm).

Interleukin-12 (IL-12) is a heterodimeric pro-inflammatory cytokine best known for inducing IFN-γ production in T cells and NK cells. IL-12 consists of two disulfide-linked subunits (p35 and p40) that regulate the biosynthesis of IL-12p70. The effects of IL-12 are mediated through its high-affinity receptor that contains two subunits: IL-12Rβ1 and IL-12Rβ2. IL-12 is produced by macrophages and B cells and has been shown to have multiple effects on T cells and natural killer (NK) cells. While mouse IL-12 is active on both human and mouse cells, human IL-12 is not active on mouse cells.

Specification

Item Number P162016S / P162016E
Specification 48 T / 96 T
Detection Range 31.25-2000 pg/mL
Detection Method Sandwich ELISA
Detection Species Mouse
Detection Time 4.5 hours
Sensitivity 7.19 pg/mL
Dilution Linearity 96 - 124%
Recovery Rate 82 - 122%
Intra-assay Variability 3.2%

Components

Component Number Component Name Storage Temperature P162016S P162016E
P162016-A Plate 2~8℃ 48 T 96 T
P162016-B Standard 2~8℃ 1 tube 2 tubes
P162016-C Detection Antibody 2~8℃ 120 μL 240 μL
P162016-D Enzyme Conjugate 2~8℃(Avoid Light) 30 μL 60 μL
P162016-E 5× Dilution Buffer 2~8℃ 8 mL 15 mL
P162016-F 20× Wash Buffer 2~8℃ 15 mL 30 mL
P162016-G Substrate Solution 2~8℃(Avoid Light) 8 mL 15 mL
P162016-H Stop Solution Room Temperature 5 mL 10 mL
P162016-I Plate Sealers Room Temperature 3 pieces 5 pieces

Storage

The assay kit can be stored at 2~8℃. Alternatively, the reagents can be stored according to the storage conditions provided in the component information to avoid contamination and repeated freeze-thaw cycles. Diluted working solutions should be used immediately and not reused. The shelf life is 1 year.

Table 1 Reagent Storage Table After First Use

     Material Name Storage Conditions
    Plate   Unused strips can be returned to the aluminum foil bag, tightly sealed, and stored at 2~8°C to avoid moisture absorption.
    Standard Use within 48 hours after dissolution, store at 2~8°C to avoid contamination.
    Detection Antibody Use within 48 hours after dilution, store at 2~8°C to avoid contamination.
    Enzyme conjugate Use within 48 hours after dilution, store at 2~8°C to avoid contamination.
    5×Dilution Buffer Store at 2~8°C for 1 month, avoiding contamination.
    20×Wash Buffer Store at 2~8°C for 1 month, avoiding contamination.
    Substrate solution Store at 2~8°C for 1 month, avoiding light exposure.
    Stop Solution Can be stored at room temperature.
    Plate Sealers Can be stored at room temperature.

                                      

    Documents:

    Mouse IL-12 p70 ELISA Kit_P162016

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    SKU: P162016E

    $900.00$375.00

        • Tell a unique detail about this product

        Description

        Mouse IL-12p70 ELISA (Enzyme-Linked Immunosorbent Assay) Kit is an in vitro enzyme-linked immunosorbent assay kit used for quantitative determination of IL-12p70 in serum, plasma, and cell culture supernatants. Specific anti-IL-12p70 antibodies are pre-coated onto high-affinity enzyme plates. Standards and samples are added to the wells of the enzyme plate, and after incubation, IL-12p70 present in the sample binds to the solid-phase antibody. After washing to remove unbound substances, a detection antibody is added for binding and incubated, followed by washing, and then the enzyme complex (Streptavidin-HRP) is added for binding and incubated. After washing, a colorimetric substrateTMB is added for color development, avoiding light. The intensity of the color reaction is proportional to the concentration of IL-12p70 in the sample. The reaction is terminated by adding a stop solution, and the absorbance is measured at 450 nm wavelength (with a reference wavelength of 570 - 630 nm).

        Interleukin-12 (IL-12) is a heterodimeric pro-inflammatory cytokine best known for inducing IFN-γ production in T cells and NK cells. IL-12 consists of two disulfide-linked subunits (p35 and p40) that regulate the biosynthesis of IL-12p70. The effects of IL-12 are mediated through its high-affinity receptor that contains two subunits: IL-12Rβ1 and IL-12Rβ2. IL-12 is produced by macrophages and B cells and has been shown to have multiple effects on T cells and natural killer (NK) cells. While mouse IL-12 is active on both human and mouse cells, human IL-12 is not active on mouse cells.

        Specification

        Item Number P162016S / P162016E
        Specification 48 T / 96 T
        Detection Range 31.25-2000 pg/mL
        Detection Method Sandwich ELISA
        Detection Species Mouse
        Detection Time 4.5 hours
        Sensitivity 7.19 pg/mL
        Dilution Linearity 96 - 124%
        Recovery Rate 82 - 122%
        Intra-assay Variability 3.2%

        Components

        Component Number Component Name Storage Temperature P162016S P162016E
        P162016-A Plate 2~8℃ 48 T 96 T
        P162016-B Standard 2~8℃ 1 tube 2 tubes
        P162016-C Detection Antibody 2~8℃ 120 μL 240 μL
        P162016-D Enzyme Conjugate 2~8℃(Avoid Light) 30 μL 60 μL
        P162016-E 5× Dilution Buffer 2~8℃ 8 mL 15 mL
        P162016-F 20× Wash Buffer 2~8℃ 15 mL 30 mL
        P162016-G Substrate Solution 2~8℃(Avoid Light) 8 mL 15 mL
        P162016-H Stop Solution Room Temperature 5 mL 10 mL
        P162016-I Plate Sealers Room Temperature 3 pieces 5 pieces

        Storage

        The assay kit can be stored at 2~8℃. Alternatively, the reagents can be stored according to the storage conditions provided in the component information to avoid contamination and repeated freeze-thaw cycles. Diluted working solutions should be used immediately and not reused. The shelf life is 1 year.

        Table 1 Reagent Storage Table After First Use

           Material Name Storage Conditions
          Plate   Unused strips can be returned to the aluminum foil bag, tightly sealed, and stored at 2~8°C to avoid moisture absorption.
          Standard Use within 48 hours after dissolution, store at 2~8°C to avoid contamination.
          Detection Antibody Use within 48 hours after dilution, store at 2~8°C to avoid contamination.
          Enzyme conjugate Use within 48 hours after dilution, store at 2~8°C to avoid contamination.
          5×Dilution Buffer Store at 2~8°C for 1 month, avoiding contamination.
          20×Wash Buffer Store at 2~8°C for 1 month, avoiding contamination.
          Substrate solution Store at 2~8°C for 1 month, avoiding light exposure.
          Stop Solution Can be stored at room temperature.
          Plate Sealers Can be stored at room temperature.

                                            

          Documents:

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