Complete Adapter

The Complete Adapter Kit for Illumina is a specialized kit designed for DNA library construction on the IlluminaTM high-throughput sequencing platform. The kit consists of 2 Sets, each containing 48 different single-indexed DNA Adapters, totaling 96 unique single-indexed DNA Adapters across both sets. Set 1 includes DNA Adapter 49-96, providing 48 unique single-index adapters. All reagents included in the kit have undergone rigorous quality control and functional validation to ensure maximum stability and reproducibility in library construction. Specifications Cat.No. N210707S / N210707M Size 48×1 T / 48×4 T Components Name N210707S N210707M DNA Adapter 49-96 5 μL each 14 μL each Storage Store at -25 °C to -15 °C.Valid for 18 months. Notes 1. For your safety and health, wear a lab coat and disposable gloves while handling the reagents. 2. The DNA Adapter concentration is 15 μM. The required volume per library may vary depending on the library prep kit and the amount of starting template used. 3. Each DNA Adapter contains a Universal Adapter and provides one index sequence tag for sample discrimination during high-throughput sequencing. 4. The kit is divided into two Sets, with each set containing 48 different single-indexed DNA Adapters. Together, the two sets can construct libraries with 96 unique single-indexed tags. 5. Do not heat the adapters; allow them to dissolve slowly at room temperature. Optimal lab temperature is 20–25 °C. Avoid repeated freeze-thaw cycles. We recommend aliquoting and storing at short-term 4 °C. 6. The structure of the sequencing library constructed using the Hieff NGSTM Complete Adapter Kit for IlluminaTM is illustrated below: 7. This product is intended for research use only. Sequence Information DNA Adapter for IlluminaTM: Universal Adapter: 5’-AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT-3’ Index Adapter: 5’-GATCGGAAGAGCACACGTCTGAACTCCAGTCACXXXXXXXX1ATCTCGTATGCCGTCTTCTGCTTG-3’ XXXXXXXX1 represents an 8-bp index sequence. Each adapter contains a unique index sequence as shown in the table below. When preparing the Sample Sheet for sequencing, only the 8-bp index sequence needs to be entered. DNA Adapter Sequence DNA Adapter Sequence DNA Adapter Sequence DNA Adapter Sequence DNA Adapter 01 AACGTGAT DNA Adapter 25 AGATCGCA DNA Adapter 49 GATAGACA DNA Adapter 73 AATGTTGC DNA Adapter 02 AAACATCG DNA Adapter 26 AGCAGGAA DNA Adapter 50 GCCACATA DNA Adapter 74 ACACGACC DNA Adapter 03 ATGCCTAA DNA Adapter 27 AGTCACTA DNA Adapter 51 GCGAGTAA DNA Adapter 75 ACAGATTC DNA Adapter 04 AGTGGTCA DNA Adapter 28 ATCCTGTA DNA Adapter 52 GCTAACGA DNA Adapter 76 AGATGTAC DNA Adapter 05 ACCACTGT DNA Adapter 29 ATTGAGGA DNA Adapter 53 GCTCGGTA DNA Adapter 77 AGCACCTC DNA Adapter 06 ACATTGGC DNA Adapter 30 CAACCACA DNA Adapter 54 GGAGAACA DNA Adapter 78 AGCCATGC DNA Adapter 07 CAGATCTG DNA Adapter 31 GACTAGTA DNA Adapter 55 GGTGCGAA DNA Adapter 79 AGGCTAAC DNA Adapter 08 CATCAAGT DNA Adapter 32 CAATGGAA DNA Adapter 56 GTACGCAA DNA Adapter 80 ATAGCGAC DNA Adapter 09 CGCTGATC DNA Adapter 33 CACTTCGA DNA Adapter 57 GTCGTAGA DNA Adapter 81 ATCATTCC DNA Adapter 10 ACAAGCTA DNA Adapter 34 CAGCGTTA DNA Adapter 58 GTCTGTCA DNA Adapter 82 ATTGGCTC DNA Adapter 11 CTGTAGCC DNA Adapter 35 CATACCAA DNA Adapter 59 GTGTTCTA DNA Adapter 83 CAAGGAGC DNA Adapter 12 AGTACAAG DNA Adapter 36 CCAGTTCA DNA Adapter 60 TAGGATGA DNA Adapter 84 CACCTTAC DNA Adapter 13 AACAACCA DNA Adapter 37 CCGAAGTA DNA Adapter 61 TATCAGCA DNA Adapter 85 CCATCCTC DNA Adapter 14 AACCGAGA DNA Adapter 38 CCGTGAGA DNA Adapter 62 TCCGTCTA DNA Adapter 86 CCGACAAC DNA Adapter 15 AACGCTTA DNA Adapter 39 CCTCCTGA DNA Adapter 63 TCTTCACA DNA Adapter 87 CCTAATCC DNA Adapter 16 AAGACGGA DNA Adapter 40 CGAACTTA DNA Adapter 64 TGAAGAGA DNA Adapter 88 CCTCTATC DNA Adapter 17 AAGGTACA DNA Adapter 41 CGACTGGA DNA Adapter 65 TGGAACAA DNA Adapter 89 CGACACAC DNA Adapter 18 ACACAGAA DNA Adapter 42 CGCATACA DNA Adapter 66 TGGCTTCA DNA Adapter 90 CGGATTGC DNA Adapter 19 ACAGCAGA DNA Adapter 43 CTCAATGA DNA Adapter 67 TGGTGGTA DNA Adapter 91 CTAAGGTC DNA Adapter 20 ACCTCCAA DNA Adapter 44 CTGAGCCA DNA Adapter 68 TTCACGCA DNA Adapter 92 GAACAGGC DNA Adapter 21 ACGCTCGA DNA Adapter 45 CTGGCATA DNA Adapter 69 AACTCACC DNA Adapter 93 GACAGTGC DNA Adapter 22 ACGTATCA DNA Adapter 46 GAATCTGA DNA Adapter 70 AAGAGATC DNA Adapter 94 GAGTTAGC DNA Adapter 23 ACTATGCA DNA Adapter 47 CAAGACTA DNA Adapter 71 AAGGACAC DNA Adapter 95 GATGAATC DNA Adapter 24 AGAGTCAA DNA Adapter 48 GAGCTGAA DNA Adapter 72 AATCCGTC DNA Adapter 96 GCCAAGAC Documents: Manuals

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The Complete Adapter Kit for Illumina is a specialized kit designed for DNA library construction on the IlluminaTM high-throughput sequencing platform. The kit consists of 2 Sets, each containing 48 different single-indexed DNA Adapters, totaling 96 unique single-indexed DNA Adapters across both sets. Set 1 includes DNA Adapter 01–48, providing 48 unique single-index adapters. All reagents included in the kit have undergone rigorous quality control and functional validation to ensure maximum stability and reproducibility in library construction. Specifications Cat.No. N210706S / N210706M Size 48×1 T / 48×4 T Components Name N210706S N210706M DNA Adapter 01-48 5 μL each 14 μL each Storage Store at -25 °C ~ -15 °C.Valid for 18 months. Notes 1. For your safety and health, wear a lab coat and disposable gloves while handling the reagents. 2. The DNA Adapter concentration is 15 μM. The required volume per library may vary depending on the library prep kit and the amount of starting template used. 3. Each DNA Adapter contains a Universal Adapter and provides one index sequence tag for sample discrimination during high-throughput sequencing. 4. The kit is divided into two Sets, with each set containing 48 different single-indexed DNA Adapters. Together, the two sets can construct libraries with 96 unique single-indexed tags. 5. Do not heat the adapters; allow them to dissolve slowly at room temperature. Optimal lab temperature is 20–25 °C. Avoid repeated freeze-thaw cycles. We recommend aliquoting and storing at short-term 4 °C. 6. The structure of the sequencing library constructed using the Hieff NGSTM Complete Adapter Kit for IlluminaTM is illustrated below: 7. This product is intended for research use only. Sequence Information DNA Adapter for IlluminaTM: Universal Adapter: 5’-AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT-3’ Index Adapter: 5’-GATCGGAAGAGCACACGTCTGAACTCCAGTCACXXXXXXXX1ATCTCGTATGCCGTCTTCTGCTTG-3’ XXXXXXXX1 represents an 8-bp index sequence. Each adapter contains a unique index sequence as shown in the table below. When preparing the Sample Sheet for sequencing, only the 8-bp index sequence needs to be entered. DNA Adapter Sequence DNA Adapter Sequence DNA Adapter Sequence DNA Adapter Sequence DNA Adapter 01 AACGTGAT DNA Adapter 25 AGATCGCA DNA Adapter 49 GATAGACA DNA Adapter 73 AATGTTGC DNA Adapter 02 AAACATCG DNA Adapter 26 AGCAGGAA DNA Adapter 50 GCCACATA DNA Adapter 74 ACACGACC DNA Adapter 03 ATGCCTAA DNA Adapter 27 AGTCACTA DNA Adapter 51 GCGAGTAA DNA Adapter 75 ACAGATTC DNA Adapter 04 AGTGGTCA DNA Adapter 28 ATCCTGTA DNA Adapter 52 GCTAACGA DNA Adapter 76 AGATGTAC DNA Adapter 05 ACCACTGT DNA Adapter 29 ATTGAGGA DNA Adapter 53 GCTCGGTA DNA Adapter 77 AGCACCTC DNA Adapter 06 ACATTGGC DNA Adapter 30 CAACCACA DNA Adapter 54 GGAGAACA DNA Adapter 78 AGCCATGC DNA Adapter 07 CAGATCTG DNA Adapter 31 GACTAGTA DNA Adapter 55 GGTGCGAA DNA Adapter 79 AGGCTAAC DNA Adapter 08 CATCAAGT DNA Adapter 32 CAATGGAA DNA Adapter 56 GTACGCAA DNA Adapter 80 ATAGCGAC DNA Adapter 09 CGCTGATC DNA Adapter 33 CACTTCGA DNA Adapter 57 GTCGTAGA DNA Adapter 81 ATCATTCC DNA Adapter 10 ACAAGCTA DNA Adapter 34 CAGCGTTA DNA Adapter 58 GTCTGTCA DNA Adapter 82 ATTGGCTC DNA Adapter 11 CTGTAGCC DNA Adapter 35 CATACCAA DNA Adapter 59 GTGTTCTA DNA Adapter 83 CAAGGAGC DNA Adapter 12 AGTACAAG DNA Adapter 36 CCAGTTCA DNA Adapter 60 TAGGATGA DNA Adapter 84 CACCTTAC DNA Adapter 13 AACAACCA DNA Adapter 37 CCGAAGTA DNA Adapter 61 TATCAGCA DNA Adapter 85 CCATCCTC DNA Adapter 14 AACCGAGA DNA Adapter 38 CCGTGAGA DNA Adapter 62 TCCGTCTA DNA Adapter 86 CCGACAAC DNA Adapter 15 AACGCTTA DNA Adapter 39 CCTCCTGA DNA Adapter 63 TCTTCACA DNA Adapter 87 CCTAATCC DNA Adapter 16 AAGACGGA DNA Adapter 40 CGAACTTA DNA Adapter 64 TGAAGAGA DNA Adapter 88 CCTCTATC DNA Adapter 17 AAGGTACA DNA Adapter 41 CGACTGGA DNA Adapter 65 TGGAACAA DNA Adapter 89 CGACACAC DNA Adapter 18 ACACAGAA DNA Adapter 42 CGCATACA DNA Adapter 66 TGGCTTCA DNA Adapter 90 CGGATTGC DNA Adapter 19 ACAGCAGA DNA Adapter 43 CTCAATGA DNA Adapter 67 TGGTGGTA DNA Adapter 91 CTAAGGTC DNA Adapter 20 ACCTCCAA DNA Adapter 44 CTGAGCCA DNA Adapter 68 TTCACGCA DNA Adapter 92 GAACAGGC DNA Adapter 21 ACGCTCGA DNA Adapter 45 CTGGCATA DNA Adapter 69 AACTCACC DNA Adapter 93 GACAGTGC DNA Adapter 22 ACGTATCA DNA Adapter 46 GAATCTGA DNA Adapter 70 AAGAGATC DNA Adapter 94 GAGTTAGC DNA Adapter 23 ACTATGCA DNA Adapter 47 CAAGACTA DNA Adapter 71 AAGGACAC DNA Adapter 95 GATGAATC DNA Adapter 24 AGAGTCAA DNA Adapter 48 GAGCTGAA DNA Adapter 72 AATCCGTC DNA Adapter 96 GCCAAGAC Documents: Manuals

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