Description
The Complete Adapter Kit for Illumina is a specialized kit designed for DNA library construction on the IlluminaTM high-throughput sequencing platform. The kit consists of 2 Sets, each containing 48 different single-indexed DNA Adapters, totaling 96 unique single-indexed DNA Adapters across both sets. Set 1 includes DNA Adapter 49-96, providing 48 unique single-index adapters. All reagents included in the kit have undergone rigorous quality control and functional validation to ensure maximum stability and reproducibility in library construction.
Specifications
|
Cat.No.
|
N210707S / N210707M
|
|
Size
|
48×1 T / 48×4 T
|
Components
|
Name
|
N210707S
|
N210707M
|
|
DNA Adapter 49-96
|
5 μL each
|
14 μL each
|
Storage
Store at -25 °C to -15 °C.Valid for 18 months.
Notes
1. For your safety and health, wear a lab coat and disposable gloves while handling the reagents.
2. The DNA Adapter concentration is 15 μM. The required volume per library may vary depending on the library prep kit and the amount of starting template used.
3. Each DNA Adapter contains a Universal Adapter and provides one index sequence tag for sample discrimination during high-throughput sequencing.
4. The kit is divided into two Sets, with each set containing 48 different single-indexed DNA Adapters. Together, the two sets can construct libraries with 96 unique single-indexed tags.
5. Do not heat the adapters; allow them to dissolve slowly at room temperature. Optimal lab temperature is 20–25 °C. Avoid repeated freeze-thaw cycles. We recommend aliquoting and storing at short-term 4 °C.
6. The structure of the sequencing library constructed using the Hieff NGSTM Complete Adapter Kit for IlluminaTM is illustrated below:
7. This product is intended for research use only.
Sequence Information
DNA Adapter for IlluminaTM:
Universal Adapter:
5’-AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT-3’
Index Adapter:
5’-GATCGGAAGAGCACACGTCTGAACTCCAGTCACXXXXXXXX1ATCTCGTATGCCGTCTTCTGCTTG-3’
XXXXXXXX1 represents an 8-bp index sequence. Each adapter contains a unique index sequence as shown in the table below. When preparing the Sample Sheet for sequencing, only the 8-bp index sequence needs to be entered.
|
DNA Adapter
|
Sequence
|
DNA Adapter
|
Sequence
|
DNA Adapter
|
Sequence
|
DNA Adapter
|
Sequence
|
|
DNA Adapter 01
|
AACGTGAT
|
DNA Adapter 25
|
AGATCGCA
|
DNA Adapter 49
|
GATAGACA
|
DNA Adapter 73
|
AATGTTGC
|
|
DNA Adapter 02
|
AAACATCG
|
DNA Adapter 26
|
AGCAGGAA
|
DNA Adapter 50
|
GCCACATA
|
DNA Adapter 74
|
ACACGACC
|
|
DNA Adapter 03
|
ATGCCTAA
|
DNA Adapter 27
|
AGTCACTA
|
DNA Adapter 51
|
GCGAGTAA
|
DNA Adapter 75
|
ACAGATTC
|
|
DNA Adapter 04
|
AGTGGTCA
|
DNA Adapter 28
|
ATCCTGTA
|
DNA Adapter 52
|
GCTAACGA
|
DNA Adapter 76
|
AGATGTAC
|
|
DNA Adapter 05
|
ACCACTGT
|
DNA Adapter 29
|
ATTGAGGA
|
DNA Adapter 53
|
GCTCGGTA
|
DNA Adapter 77
|
AGCACCTC
|
|
DNA Adapter 06
|
ACATTGGC
|
DNA Adapter 30
|
CAACCACA
|
DNA Adapter 54
|
GGAGAACA
|
DNA Adapter 78
|
AGCCATGC
|
|
DNA Adapter 07
|
CAGATCTG
|
DNA Adapter 31
|
GACTAGTA
|
DNA Adapter 55
|
GGTGCGAA
|
DNA Adapter 79
|
AGGCTAAC
|
|
DNA Adapter 08
|
CATCAAGT
|
DNA Adapter 32
|
CAATGGAA
|
DNA Adapter 56
|
GTACGCAA
|
DNA Adapter 80
|
ATAGCGAC
|
|
DNA Adapter 09
|
CGCTGATC
|
DNA Adapter 33
|
CACTTCGA
|
DNA Adapter 57
|
GTCGTAGA
|
DNA Adapter 81
|
ATCATTCC
|
|
DNA Adapter 10
|
ACAAGCTA
|
DNA Adapter 34
|
CAGCGTTA
|
DNA Adapter 58
|
GTCTGTCA
|
DNA Adapter 82
|
ATTGGCTC
|
|
DNA Adapter 11
|
CTGTAGCC
|
DNA Adapter 35
|
CATACCAA
|
DNA Adapter 59
|
GTGTTCTA
|
DNA Adapter 83
|
CAAGGAGC
|
|
DNA Adapter 12
|
AGTACAAG
|
DNA Adapter 36
|
CCAGTTCA
|
DNA Adapter 60
|
TAGGATGA
|
DNA Adapter 84
|
CACCTTAC
|
|
DNA Adapter 13
|
AACAACCA
|
DNA Adapter 37
|
CCGAAGTA
|
DNA Adapter 61
|
TATCAGCA
|
DNA Adapter 85
|
CCATCCTC
|
|
DNA Adapter 14
|
AACCGAGA
|
DNA Adapter 38
|
CCGTGAGA
|
DNA Adapter 62
|
TCCGTCTA
|
DNA Adapter 86
|
CCGACAAC
|
|
DNA Adapter 15
|
AACGCTTA
|
DNA Adapter 39
|
CCTCCTGA
|
DNA Adapter 63
|
TCTTCACA
|
DNA Adapter 87
|
CCTAATCC
|
|
DNA Adapter 16
|
AAGACGGA
|
DNA Adapter 40
|
CGAACTTA
|
DNA Adapter 64
|
TGAAGAGA
|
DNA Adapter 88
|
CCTCTATC
|
|
DNA Adapter 17
|
AAGGTACA
|
DNA Adapter 41
|
CGACTGGA
|
DNA Adapter 65
|
TGGAACAA
|
DNA Adapter 89
|
CGACACAC
|
|
DNA Adapter 18
|
ACACAGAA
|
DNA Adapter 42
|
CGCATACA
|
DNA Adapter 66
|
TGGCTTCA
|
DNA Adapter 90
|
CGGATTGC
|
|
DNA Adapter 19
|
ACAGCAGA
|
DNA Adapter 43
|
CTCAATGA
|
DNA Adapter 67
|
TGGTGGTA
|
DNA Adapter 91
|
CTAAGGTC
|
|
DNA Adapter 20
|
ACCTCCAA
|
DNA Adapter 44
|
CTGAGCCA
|
DNA Adapter 68
|
TTCACGCA
|
DNA Adapter 92
|
GAACAGGC
|
|
DNA Adapter 21
|
ACGCTCGA
|
DNA Adapter 45
|
CTGGCATA
|
DNA Adapter 69
|
AACTCACC
|
DNA Adapter 93
|
GACAGTGC
|
|
DNA Adapter 22
|
ACGTATCA
|
DNA Adapter 46
|
GAATCTGA
|
DNA Adapter 70
|
AAGAGATC
|
DNA Adapter 94
|
GAGTTAGC
|
|
DNA Adapter 23
|
ACTATGCA
|
DNA Adapter 47
|
CAAGACTA
|
DNA Adapter 71
|
AAGGACAC
|
DNA Adapter 95
|
GATGAATC
|
|
DNA Adapter 24
|
AGAGTCAA
|
DNA Adapter 48
|
GAGCTGAA
|
DNA Adapter 72
|
AATCCGTC
|
DNA Adapter 96
|
GCCAAGAC
|
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