Description
The NGS DNA Library Prep Kit (Enzymatic) is a next-generation enzymatic library preparation kit compatible with both IlluminaTM and MGITM high-throughput sequencing platforms. Compared to traditional methods, this kit eliminates the need for cumbersome sonication by using high-quality fragmentation enzymes. The fragmentation and end-repair with dA-tailing modules are combined into a single step, significantly reducing the time and cost of library preparation. It is suitable for input amounts ranging from 1 ng to 1 μg of standard plant and animal genomic DNA, microbial genomic DNA, etc. The enzyme produces uniform fragmentation across different species with minimal species-specific variation. DNA fragmentation, end-repair and dA-tailing are all performed in a single tube. The kit is compatible with both IlluminaTM and MGITM adapters and primers for sequencing on both platforms.
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Applicable to genomic DNA and full-length cDNA input amounts of 1 ng to 1 μg
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High-quality fragmentation enzyme enables random dsDNA cleavage with minimal bias
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Fragmentation, end repair, and dA-tailing in a single step
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High-fidelity enzyme with strong amplification efficiency significantly improves library quality and yield
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Suitable for a wide range of species
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Strict batch performance and stability control
Specifications
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Cat.No.
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N210001E / N210001S / N210001M /N210001L
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Size
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8 T / 24 T / 96 T /96 T(Plate-Packaging）
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Components
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Components No.
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Name
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N210001E
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N210001S
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N210001M
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N210001L*
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N210001-A
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SmearaseTM Buffer 4.0
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80 μL
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240 μL
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960 μL
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8×150 μL
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N210001-B
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SmearaseTM Enzyme 4.0
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80 μL
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240 μL
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960 μL
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8×150 μL
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N210001-C
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Ligation Enhancer 4.0
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240 μL
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720 μL
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3×960 μL
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8×420 μL
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N210001-D
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Rapid DNA Ligase 4.0
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80 μL
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240 μL
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2×480 μL
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8×140 μL
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N210001-E
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2×Ultima HF Amplification Mix
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200 μL
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600 μL
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3×800 μL
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8×340 μL
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【Note】: When using incomplete adapters, no primer mix is required. However, for full-length adapters, a primer mix is essential and must be purchased separately. This kit is compatible with both Illumina™ and MGI™ platforms, but requires platform-specific primer mixes: Cat# N210701 for Illumina™; Cat# N210781 for MGI™.
*For plate format (automated 96T), refer to the layout diagram below:
Storage
This product should be stored at -25~-15℃ for 1 year.
Workflow
Documents：
Related Blog:
Enzymatic Fragmentation DNA Library Prep Kits: Empowering Multi-Disciplinary Research Applications
