mRNA

Product description Firefly luciferase facilitates the conversion of luciferin to oxyluciferin in conditions where ATP, magnesium ions, and oxygen are present, resulting in the emission of bioluminescence at a wavelength of about 560 nm, detectable with a luminometer. CleanRNA™ Firefly Luciferase-mRNA (low dsRNA) is an mRNA is an mRNA that has been modified with a 5' cap1, a 3' poly(A) tail, and N1-Methyl-Pseudouridine Triphosphate (N1-Me-Pseudo UTP). The product is created through an in vitro transcription process using CleaScrip™ T7 RNA polymerase, which generates ultralow dsRNA content in the mRNA solution, thereby improving the in vivo performance of the Firefly Luciferase-mRNA.   Specifications Catalog Number N120011S/N120011M Specifications 0.5 mg/1 mg mRNA length 2078 nt Concentration 1 mg/mL Capping efficiency 100% dsRNA ＜0.1 ng/μg Storage buffer 1 mM sodium citrate   Components Component Number Component Name Storage Temperature N120011S N120011M N120011 CleanRNA™ Firefly Luciferase-mRNA (low dsRNA) -80°C 0.5 mg 1 mg Integrity tested by CE (capillary electrophoresis) Capping efficiency tested by MS Shipping and Storage The products are shipped with dry ice and can be stored at -80℃ for two year.   Notes 1. Upon arrival, immediately store the samples at -80°C and avoid repeated freeze-thaw. 2. For the first use, be sure to wear disposable gloves, dissolve the samples on ice, and use RNase-free reagents and consumables to avoid contamination. 3. After the initial dissolution of the samples, they can be gently centrifuged and then aliquoted, avoiding repeated freezing and thawing. EN-N120011-Manual

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Product description eGFP is frequently utilized as a fluorescent tag for the observation and monitoring of protein expression and location within living cells. When eGFP-mRNA is transfected into cells, the enhanced green fluorescent protein (eGFP) is produced within the cellular environment. CleanRNA™ eGFP-mRNA (low dsRNA) is an mRNA that has been modified with a 5' cap1, a 3' poly(A) tail, and N1-Methyl-Pseudouridine Triphosphate (N1-Me-Pseudo UTP). The product is created through an in vitro transcription process using CleaScrip™ T7 RNA polymerase, which generates ultralow sRNA content in the mRNA solution, thus improving the in vivo performance of eGFP-mRNA.  Specifications Catalog Number N120012S/N120012M Specifications 0.5 mg/1 mg mRNA length 995 nt Concentration 1 mg/mL Capping efficiency 100% dsRNA ＜0.1 ng/μg Storage buffer 1 mM sodium citrate   Components Component Number Component Name Storage Temperature N120011S N120011M N120012 CleanRNA™ eGFP-mRNA (low dsRNA) -80°C 0.5 mg 1 mg Integrity tested by CE (capillary electrophoresis) Capping efficiency tested by MS Shipping and Storage The products are shipped with dry ice and can be stored at -80℃ for two year. Notes 1. Upon arrival, immediately store the samples at -80°C and avoid repeated freeze-thaw. 2. For the first use, be sure to wear disposable gloves, dissolve the samples on ice, and use RNase-free reagents and consumables to avoid contamination. 3. After the initial dissolution of the samples, they can be gently centrifuged and then aliquoted, avoiding repeated freezing and thawing. EN-N120012-Manual

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