Description
IL- 36 alpha, previously called IL- 1F6 and FIL1 epsilon (family of IL- 1 member epsilon), is a member of the IL- 1 family which includes IL- 1 beta, IL- 1 alpha, IL- 1ra, IL- 18, and novel family members IL- 36 Ra (IL- 1F5), IL- 36 beta (IL- 1F8), IL- 36 gamma (IL- 1F9), IL- 37 (IL- 1F7) and IL- 1F10. All family members show a 12 beta - strand, beta - trefoil configuration, and are believed to have arisen from a common ancestral gene. IL- 36 alpha is an 18 kDa, 160 amino acid (aa) intracellular and secreted protein that contains no signal sequence, no prosegment and no potential N- linked glycosylation sites. It can be externalized non- specifically in response to LPS and ATP- induced activation of the P2X7 receptor. Full- length recombinant IL- 36 alpha is less active than endogenous IL- 36 alpha, but trimming of the N- termini enhances its activity. Mouse IL- 36 alpha shares 83% aa sequence identity with rat IL- 36 alpha, 54- 60% with human, rabbit, equine and bovine IL- 36 alpha, and 27- 57% aa sequence identity with other novel IL- 1 family members. IL- 36 alpha is mainly found in skin and lymphoid tissues, but also in fetal brain, trachea, stomach and intestine. It is expressed by monocytes, B and T cells. The receptor for IL- 36 alpha is a combination of IL- 1 Rrp2 (also called IL- 1 RL2 or IL- 1 R6), mainly found in epithelia and keratinocytes, and the widely expressed IL- 1 RAcP. IL- 36 alpha, beta and gamma all activate NF- kappa B and MAPK pathways in an IL- 1 Rrp2 dependent manner, and induce production of inflammatory cytokines and chemokines such as CXCL8/IL- 8. IL- 36 alpha and other family members are overexpressed in psoriatic skin lesions, and transgenic overexpression of IL- 36 alpha in skin keratinocytes produces epidermal hyperplasia. IL- 36 alpha is present in kidney tubule epithelia; it is highly overexpressed in tubulointerstitial lesions in mouse models of chronic glomerulonephritis, lupus nephritis and diabetic nephritis. IL- 36 alpha is induced by inflammation in adipose tissue- resident alternately activated (M2) macrophages, and reduces adipocyte differentiation.
Product Properties
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Synonyms
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FIL1 epsilon, IL-1 epsilon, IL-1F6, IL-1H1
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Accession
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Q9JLA2
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GeneID
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54448
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Source
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E.coli-derived Mouse IL-36α,160aa, Met1-His160, with an N-terminal Met.
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Molecular Weight
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Approximately 18.0 kDa.
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AA Sequence
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MNKEKELRAA SPSLRHVQDL SSRVWILQNN ILTAVPRKEQ TVPVTITLLP CQYLDTLETN RGDPTYMGVQ RPMSCLFCTK DGEQPVLQLG EGNIMEMYNK KEPVKASLFY HKKSGTTSTF ESAAFPGWFI AVCSKGSCPL ILTQELGEIF ITDFEMIVVH
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Tag
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None
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Physical Appearance
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Sterile Filtered White lyophilized (freeze-dried) powder.
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Purity
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> 95% by SDS-PAGE and HPLC analyses.
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Biological Activity
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The specific activity determined by its ability in a functional ELISA. Immobilized rMuIL-36α at 1 µg/mL can bind recombinant murine IL-1 Rrp2 with a range of 0.15-5 µg/mL. Fully biologically active when compared to standard.
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Endotoxin
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< 1.0 EU per 1μg of the protein by the LAL method.
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Formulation
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Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4, 5 % trehalose.
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Reconstitution
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We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions.
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Shipping and Storage
The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
Cautions
1. Avoid repeated freeze-thaw cycles.
2. For your safety and health, please wear lab coats and disposable gloves for operation.
3. For research use only!
