Recombinant Mouse Insulin-like Growth factor-1 (Mouse IGF-1)_C230557

Description

Insulin-like Growth Factor I (IGF-I), is the dominant effector of Growth Hormone (GH) and is structurally homologous to Proinsulin. The 7.6 kDa mature IGF-I is identical between isoforms and is generated by proteolytic removal of the N- and C-terminal regions. Mature human IGF-I shares 94% and 96% amino acid (aa) sequence identity with the mouse and rat orthologs, respectively. The body growth of animals is regulated by growth hormone and IGF-I. The classical theory of this regulation is that most IGF-I in the blood originates in the liver and that body growth is controlled by the concentration of IGF-I in the blood. Meanwhile, IGF-I has been shown to enhance neuronal survival and inhibit apoptosis. IGF-1 promote the proliferation of multiple myeloma (MM) cells and protect them against dexamethasone (Dex)-induced apoptosis.

 

Product Properties

Synonyms

Somatomedin C, IGF-I, IGF-IA, Mechano growth factor, MGF
Accession
P05017
GeneID
16000

Source

E.coli-derived mouse IGF-1 protein, Gly49-Ala118.

Molecular Weight

Approximately 7.7 kDa.
AA Sequence
GPETLCGAEL VDALQFVCGP RGFYFNKPTG YGSSIRRAPQ TGIVDECCFR SCDLRRLEMY
CAPLKPTKAA
Tag
None
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.

Purity

>97% by SDS-PAGE and HPLC analyses.
Biological Activity

The ED50 as determined by a cell proliferation assay using serum free human MCF-7 cells is less than 2 ng/mL, corresponding to a specific activity of > 5.0×105 IU/mg. Fully biologically active when comparedto standard.

Endotoxin

< 0.1 EU per 1μg of the protein by the LAL method.

Formulation

Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at -20℃. Further dilutions should be made in appropriate buffered solutions.

 

Shipping and Storage

The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

Cautions

1. Avoid repeated freeze-thaw cycles.
2. For your safety and health, please wear lab coats and disposable gloves for operation.
3. For research use only!

Recombinant Mouse Insulin-like Growth factor-1 (Mouse IGF-1)_C230557

Product form

SKU: C230557E

$74.00

      • Tell a unique detail about this product

      Description

      Insulin-like Growth Factor I (IGF-I), is the dominant effector of Growth Hormone (GH) and is structurally homologous to Proinsulin. The 7.6 kDa mature IGF-I is identical between isoforms and is generated by proteolytic removal of the N- and C-terminal regions. Mature human IGF-I shares 94% and 96% amino acid (aa) sequence identity with the mouse and rat orthologs, respectively. The body growth of animals is regulated by growth hormone and IGF-I. The classical theory of this regulation is that most IGF-I in the blood originates in the liver and that body growth is controlled by the concentration of IGF-I in the blood. Meanwhile, IGF-I has been shown to enhance neuronal survival and inhibit apoptosis. IGF-1 promote the proliferation of multiple myeloma (MM) cells and protect them against dexamethasone (Dex)-induced apoptosis.

       

      Product Properties

      Synonyms

      Somatomedin C, IGF-I, IGF-IA, Mechano growth factor, MGF
      Accession
      P05017
      GeneID
      16000

      Source

      E.coli-derived mouse IGF-1 protein, Gly49-Ala118.

      Molecular Weight

      Approximately 7.7 kDa.
      AA Sequence
      GPETLCGAEL VDALQFVCGP RGFYFNKPTG YGSSIRRAPQ TGIVDECCFR SCDLRRLEMY
      CAPLKPTKAA
      Tag
      None
      Physical Appearance
      Sterile Filtered White lyophilized (freeze-dried) powder.

      Purity

      >97% by SDS-PAGE and HPLC analyses.
      Biological Activity

      The ED50 as determined by a cell proliferation assay using serum free human MCF-7 cells is less than 2 ng/mL, corresponding to a specific activity of > 5.0×105 IU/mg. Fully biologically active when comparedto standard.

      Endotoxin

      < 0.1 EU per 1μg of the protein by the LAL method.

      Formulation

      Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.

      Reconstitution

      We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at -20℃. Further dilutions should be made in appropriate buffered solutions.

       

      Shipping and Storage

      The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.
      Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

      Cautions

      1. Avoid repeated freeze-thaw cycles.
      2. For your safety and health, please wear lab coats and disposable gloves for operation.
      3. For research use only!

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