Recombinant Mouse IL-2 Protein_C230569

Description

Interleukin-2(IL-2) is a powerful immunoregulatory lymphokine produced by T-cells in response to antigenic or mitogenic stimulation. It is expressed by CD4+ and CD8+ T cells, γδ T cells, B cells, dendritic cells, and eosinophils. IL-2/IL-2R signaling is required for T- cell proliferation and other fundamental functions which are essential for the immune response. The receptor for IL-2 consists of three subunits (55 kDa IL-2Rα, 75 kDa IL-2Rβ, and 64 kDa common gamma chain γc/IL-2Rγ) that are present on the cell surface in varying preformed complexes. Mature human IL-2 shares 56% and 66% amino acid sequence identity with mouse and rat IL-2, respectively. Human and mouse IL-2 exhibit cross-species activity.

 

Product Properties

Synonyms

Interleukin-2,IL-2,T-cell Growth Factor, TCGF, Aldesleukin

Accession

P04351

GeneID

16183

Source

E.coli-derived mouse IL-2 protein, Ala21-Gln169

Molecular Weight

Approximately 17.2 kDa.

AA Sequence

APTSSSTSSS TAEAQQQQQQ QQQQQQHLEQ LLMDLQELLS RMENYRNLKL PRMLTFKFYL PKQATELKDL QCLEDELGPL RHVLDLTQSK SFQLEDAENF ISNIRVTVVK LKGSDNTFEC QFDDESATVV DFLRRWIAFC QSIISTSPQ

Tag

None

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Purity

> 95% by SDS-PAGE and HPLC analyses.

Biological Activity

The ED50 as determined by a cell proliferation assay using murine CTLL-2 cells is less than 0.2 ng/mL, corresponding to a specific activity of > 5.0 × 106 IU/mg. Fully biologically active when compared to standard.

Endotoxin

1.0 EU per 1μg of the protein by the LAL method.

Formulation

Lyophilized from a 0.2 μm filtered concentrated solution in 1 × PBS, pH 7.4.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions.

 

Shipping and Storage

The products are shipped with ice pack and can be stored at -20 ℃ for 1 year. 1 month, 2 to 8 °C under sterile conditions after reconstitution.

3 months, -20 °C under sterile conditions after reconstitution.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

Cautions

1. Avoid repeated freeze-thaw cycles.

2. For your safety and health, please wear lab coats and disposable gloves for operation.

3. For research use only!

Recombinant Mouse IL-2 Protein_C230569

Product form

SKU: C230569E

$74.00

    • Shipped today? Order within: Nov 05, 2024 17:00:00 -0500
    • Tell a unique detail about this product

    Description

    Interleukin-2(IL-2) is a powerful immunoregulatory lymphokine produced by T-cells in response to antigenic or mitogenic stimulation. It is expressed by CD4+ and CD8+ T cells, γδ T cells, B cells, dendritic cells, and eosinophils. IL-2/IL-2R signaling is required for T- cell proliferation and other fundamental functions which are essential for the immune response. The receptor for IL-2 consists of three subunits (55 kDa IL-2Rα, 75 kDa IL-2Rβ, and 64 kDa common gamma chain γc/IL-2Rγ) that are present on the cell surface in varying preformed complexes. Mature human IL-2 shares 56% and 66% amino acid sequence identity with mouse and rat IL-2, respectively. Human and mouse IL-2 exhibit cross-species activity.

     

    Product Properties

    Synonyms

    Interleukin-2,IL-2,T-cell Growth Factor, TCGF, Aldesleukin

    Accession

    P04351

    GeneID

    16183

    Source

    E.coli-derived mouse IL-2 protein, Ala21-Gln169

    Molecular Weight

    Approximately 17.2 kDa.

    AA Sequence

    APTSSSTSSS TAEAQQQQQQ QQQQQQHLEQ LLMDLQELLS RMENYRNLKL PRMLTFKFYL PKQATELKDL QCLEDELGPL RHVLDLTQSK SFQLEDAENF ISNIRVTVVK LKGSDNTFEC QFDDESATVV DFLRRWIAFC QSIISTSPQ

    Tag

    None

    Physical Appearance

    Sterile Filtered White lyophilized (freeze-dried) powder.

    Purity

    > 95% by SDS-PAGE and HPLC analyses.

    Biological Activity

    The ED50 as determined by a cell proliferation assay using murine CTLL-2 cells is less than 0.2 ng/mL, corresponding to a specific activity of > 5.0 × 106 IU/mg. Fully biologically active when compared to standard.

    Endotoxin

    1.0 EU per 1μg of the protein by the LAL method.

    Formulation

    Lyophilized from a 0.2 μm filtered concentrated solution in 1 × PBS, pH 7.4.

    Reconstitution

    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions.

     

    Shipping and Storage

    The products are shipped with ice pack and can be stored at -20 ℃ for 1 year. 1 month, 2 to 8 °C under sterile conditions after reconstitution.

    3 months, -20 °C under sterile conditions after reconstitution.

    Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

     

    Cautions

    1. Avoid repeated freeze-thaw cycles.

    2. For your safety and health, please wear lab coats and disposable gloves for operation.

    3. For research use only!

    © 2024 Arcegen, Powered by Shopify

    • American Express
    • Apple Pay
    • Diners Club
    • Discover
    • Meta Pay
    • Google Pay
    • Mastercard
    • Shop Pay
    • Visa

    Login

    Forgot your password?

    Don't have an account yet?
    Create account