Recombinant Mouse IFN-gamma Protein_C230558

Description

Interferon-gamma (IFN-γ), also known as Type II interferon or immune interferon, is a pleiotropic cytokine which is now recognised as an important modulator of the immune response. Mature human IFN-γ exists as a non-covalently linked homodimer of 20-25 kDa variably glycosylated subunits. 

 

Product Properties

Synonyms

Interferon-gamma, IFN-γ, IFG, IFI, IFNG, IFNgamma, IFN-gamma.
Accession
P01580
GeneID
15978

Source

E.coli-derived mouse IFN-γ protein, His23-Cys155.

Molecular Weight

Approximately 15.5 kDa.
AA Sequence
HGTVIESLES LNNYFNSSGI DVEEKSLFLD IWRNWQKDGD MKILQSQIIS FYLRLFEVLK DNQAISNNIS VIESHLITTF FSNSKAKKDA FMSIAKFEVN NPQVQRQAFN ELIRVVHQLL PESSLRKRKR SRC
Tag
None
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.

Purity

>96% by SDS-PAGE  analyses.
Biological Activity

The ED50 as determined by an anti-viral assay using murine L929 cells infected with encephalomyocarditis (EMC) virus is less than 0.8 ng/ml, corresponding to a specific activity of > 1.3×106 IU/mg. Fully biologically active when compared tostandard.

Endotoxin

< 1.0 EU per 1μg of the protein by the LAL method.

Formulation

Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at -20℃. Further dilutions should be made in appropriate buffered solutions.

 

Shipping and Storage

The products are shipped with ice pack and can be stored at -25~-15℃ for 1 year.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -25~-15℃ under sterile conditions after reconstitution.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

Cautions

1. Avoid repeated freeze-thaw cycles.
2. For your safety and health, please wear lab coats and disposable gloves for operation.
3. For research use only!

Recombinant Mouse IFN-gamma Protein_C230558

Product form

SKU: C230558E

$200.00

    • Shipped today? Order within: Feb 17, 2025 17:00:00 -0500
    • Tell a unique detail about this product

    Description

    Interferon-gamma (IFN-γ), also known as Type II interferon or immune interferon, is a pleiotropic cytokine which is now recognised as an important modulator of the immune response. Mature human IFN-γ exists as a non-covalently linked homodimer of 20-25 kDa variably glycosylated subunits. 

     

    Product Properties

    Synonyms

    Interferon-gamma, IFN-γ, IFG, IFI, IFNG, IFNgamma, IFN-gamma.
    Accession
    P01580
    GeneID
    15978

    Source

    E.coli-derived mouse IFN-γ protein, His23-Cys155.

    Molecular Weight

    Approximately 15.5 kDa.
    AA Sequence
    HGTVIESLES LNNYFNSSGI DVEEKSLFLD IWRNWQKDGD MKILQSQIIS FYLRLFEVLK DNQAISNNIS VIESHLITTF FSNSKAKKDA FMSIAKFEVN NPQVQRQAFN ELIRVVHQLL PESSLRKRKR SRC
    Tag
    None
    Physical Appearance
    Sterile Filtered White lyophilized (freeze-dried) powder.

    Purity

    >96% by SDS-PAGE  analyses.
    Biological Activity

    The ED50 as determined by an anti-viral assay using murine L929 cells infected with encephalomyocarditis (EMC) virus is less than 0.8 ng/ml, corresponding to a specific activity of > 1.3×106 IU/mg. Fully biologically active when compared tostandard.

    Endotoxin

    < 1.0 EU per 1μg of the protein by the LAL method.

    Formulation

    Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.

    Reconstitution

    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at -20℃. Further dilutions should be made in appropriate buffered solutions.

     

    Shipping and Storage

    The products are shipped with ice pack and can be stored at -25~-15℃ for 1 year.
    1 month, 2 to 8 °C under sterile conditions after reconstitution.
    3 months, -25~-15℃ under sterile conditions after reconstitution.
    Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

    Cautions

    1. Avoid repeated freeze-thaw cycles.
    2. For your safety and health, please wear lab coats and disposable gloves for operation.
    3. For research use only!

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