Synonyms
|
C-X3-C motif chemokine 1, CX3C membrane-anchored chemokine, Neurotactin, Small-inducible cytokine D1
|
|
|
|
|
Source
|
E.coli-derived mouse Fractalkine/CX3CL1, Gln25-Gly100.
|
Molecular Weight
|
|
AA Sequence
|
QHLGMTKCEI MCGKMTSRIP VALLIRYQLN QESCGKRAIV LETTQHRRFC ADPKEKWVQD
AMKHLDHQAA ALTKNG
|
|
|
|
Sterile Filtered White lyophilized (freeze-dried) powder.
|
Purity
|
>97% by SDS-PAGE and HPLC analyses.
|
Biological Activity
|
The ED50 as determined by a cell proliferation assay using human peripheral blood lymphocytes (PBL) is less than 0.5 μg/mL, corresponding to a specific activity of > 2000 IU/mg. Fully biologically active when compared to standard.
|
Endotoxin
|
< 0.1 EU per 1μg of the protein by the LAL method.
|
Formulation
|
Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4.
|
Reconstitution
|
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.
|