Synonyms
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Source
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E.coli-derived Mouse DHH C23II, Cys23-Gly198
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Molecular Weight
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AA Sequence
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IIGPGRGPVG RRRYVRKQLV PLLYKQFVPS MPERTLGASGPAEGRVTRGSERFRDLVPNY NPDIIFKDEE NSGADRLMTE RCKERVNALA IAVMNMWPGV RLRVTEGWDEDGHHAQDSLH YEGRALDITT SDRDRNKYGL LARLAVEAGF DWVYYESRNH IHVSVKADNS LAVRAGG
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Sterile Filtered White lyophilized (freeze-dried) powder.
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Purity
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>98% by SDS-PAGE and HPLC analyses.
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Biological Activity
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The ED50 as determined by its ability to induce alkaline phosphatase production by murine MC3T3-E1 cells is 5-20 μg/ml. Fully biologically active when compared to standard.
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Endotoxin
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< 0.1 EU per 1μg of the protein by the LAL method.
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Formulation
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Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4, 1 mM DTT, 0.05% Tween-80.
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Reconstitution
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We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.
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