Recombinant Mouse Acidic Fibroblast Growth Factor (Mouse aFGF)_C230521

Description

Mouse aFGF, encoded by the FGF1 gene, is a member of the fibroblast growth factor (FGF) family. Fibroblast growth factor was found in pituitary extracts in 1973 and then tested in a bioassay that caused fibroblasts to proliferate. After further fractionating the
extract using acidic and basic pH, two different forms have isolated that named "acidic fibroblast growth factor" (FGF-1) and "basic fibroblast growth factor" (FGF-2). Murine aFGF shares 52 % amino acid sequence identity with bFGF. Murine aFGF shares 96 %
amino acid sequence identity with human aFGF, so it exhibits considerable species crossreactivity between murine and human aFGF. In mammalian FGF receptor family has 4 members, FGFR1, FGFR2, FGFR3, and FGFR4, and 1, 2, 3 have 2 sub-types b, c
aFGF can bind and activate all 7 different FGFRs. Affinity between aFGF and its receptors can be increased by heparin or heparan sulfate proteoglycan. aFGF plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration.

 

Product Properties

Synonyms

Fibroblast growth factor 1FGF-1Acidic fibroblast growth factorHBGF-1
Accession
P61148
GeneID
14164

Source

E.coli-derived mouse Acidic Fibroblast Growth Factor protein, Phe16-Asp155

Molecular Weight

Approximately 15.8 kDa.
AA Sequence

FNLPLGNYKK PKLLYCSNGG HFLRILPDGT VDGTRDRSDQ HIQLQLSAES AGEVYIKGTE
TGQYLAMDTE GLLYGSQTPN EECLFLERLE ENHYNTYTSK KHAEKNWFVG LKKNGSCKRG
PRTHYGQKAI LFLPLPVSSD

Tag
None
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.

Purity

> 96 % by SDS-PAGE and HPLC analyses.
Biological Activity
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay
using murine balb/c 3T3 cells is less than 0.2 ng/ml, corresponding to a specific activity of > 5.0 ×
106 IU/mg in the presence of 10 μg/ml of heparin.

Endotoxin

< 1.0 EU per 1μg of the protein by the LAL method.

Formulation

Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom.
Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of
0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

 

Shipping and Storage

The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

Cautions

1. Avoid repeated freeze-thaw cycles.
2. For your safety and health, please wear lab coats and disposable gloves for operation.
3. For research use only!

Recombinant Mouse Acidic Fibroblast Growth Factor (Mouse aFGF)_C230521

Product form

SKU: C230521E

$74.00

      • Tell a unique detail about this product

      Description

      Mouse aFGF, encoded by the FGF1 gene, is a member of the fibroblast growth factor (FGF) family. Fibroblast growth factor was found in pituitary extracts in 1973 and then tested in a bioassay that caused fibroblasts to proliferate. After further fractionating the
      extract using acidic and basic pH, two different forms have isolated that named "acidic fibroblast growth factor" (FGF-1) and "basic fibroblast growth factor" (FGF-2). Murine aFGF shares 52 % amino acid sequence identity with bFGF. Murine aFGF shares 96 %
      amino acid sequence identity with human aFGF, so it exhibits considerable species crossreactivity between murine and human aFGF. In mammalian FGF receptor family has 4 members, FGFR1, FGFR2, FGFR3, and FGFR4, and 1, 2, 3 have 2 sub-types b, c
      aFGF can bind and activate all 7 different FGFRs. Affinity between aFGF and its receptors can be increased by heparin or heparan sulfate proteoglycan. aFGF plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration.

       

      Product Properties

      Synonyms

      Fibroblast growth factor 1FGF-1Acidic fibroblast growth factorHBGF-1
      Accession
      P61148
      GeneID
      14164

      Source

      E.coli-derived mouse Acidic Fibroblast Growth Factor protein, Phe16-Asp155

      Molecular Weight

      Approximately 15.8 kDa.
      AA Sequence

      FNLPLGNYKK PKLLYCSNGG HFLRILPDGT VDGTRDRSDQ HIQLQLSAES AGEVYIKGTE
      TGQYLAMDTE GLLYGSQTPN EECLFLERLE ENHYNTYTSK KHAEKNWFVG LKKNGSCKRG
      PRTHYGQKAI LFLPLPVSSD

      Tag
      None
      Physical Appearance
      Sterile Filtered White lyophilized (freeze-dried) powder.

      Purity

      > 96 % by SDS-PAGE and HPLC analyses.
      Biological Activity
      Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay
      using murine balb/c 3T3 cells is less than 0.2 ng/ml, corresponding to a specific activity of > 5.0 ×
      106 IU/mg in the presence of 10 μg/ml of heparin.

      Endotoxin

      < 1.0 EU per 1μg of the protein by the LAL method.

      Formulation

      Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4.

      Reconstitution

      We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom.
      Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of
      0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

       

      Shipping and Storage

      The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.
      Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

       

      Cautions

      1. Avoid repeated freeze-thaw cycles.
      2. For your safety and health, please wear lab coats and disposable gloves for operation.
      3. For research use only!

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