Description
Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells.Human Vimentin shares 97-98% aa identity with mouse, rat, ovine, bovine and canine Vimentin. Vimentin is involved in positioning autophagosomes, lysosomes and the Golgi complex within the cell. Vimentin helps maintain the lipid composition of cellular membranes, and caspase cleavage of Vimentin is a key event in apoptosis.
Product Properties
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Synonyms
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Accession
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Source
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E.coli-derived Human Vimentin, Ser2-Glu466.
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Molecular Weight
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AA Sequence
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STRSVSSSSY RRMFGGPGTA SRPSSSRSYV TTSTRTYSLG SALRPSTSRS LYASSPGGVY ATRSSAVRLR SSVPGVRLLQ DSVDFSLADA INTEFKNTRT NEKVELQELN DRFANYIDKV RFLEQQNKIL LAELEQLKGQ GKSRLGDLYE EEMRELRRQV DQLTNDKARV EVERDNLAED IMRLREKLQE EMLQREEAEN TLQSFRQDVD NASLARLDLE RKVESLQEEI AFLKKLHEEE
IQELQAQIQE QHVQIDVDVS KPDLTAALRD VRQQYESVAA KNLQEAEEWY KSKFADLSEA
ANRNNDALRQ AKQESTEYRR QVQSLTCEVD ALKGTNESLE RQMREMEENF AVEAANYQDT
IGRLQDEIQN MKEEMARHLR EYQDLLNVKM ALDIEIATYR KLLEGEESRI SLPLPNFSSL NLRETNLDSL PLVDTHSKRT LLIKTVETRD GQVINETSQH HDDLE
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Sterile Filtered White lyophilized (freeze-dried) powder.
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Purity
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> 95% by SDS-PAGE and HPLC analyses.
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Endotoxin
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< 0.1 EU per 1μg of the protein by the LAL method.
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Formulation
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Lyophilized from a 0.2 µm filtered solution in 30 % Acetonitrile and 0.1 % TFA.
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Reconstitution
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We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom.
Reconstitute in 4 mM HCl to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.
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Shipping and Storage
The products are shipped with ice pack and can be stored at -20℃ for 1 year.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 °C under sterile conditions after reconstitution.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
Cautions
1. Avoid repeated freeze-thaw cycles.
2. For your safety and health, please wear lab coats and disposable gloves for operation.
3. For research use only.
