Recombinant Human Sonic Hedgehog N-Terminus (Human SHH)_C230493

Description

Shh (Sonic Hedgehog protein) is a signaling molecule and a member of the Hedgehog (Hh) family. It plays a crucial role in physiological processes such as embryonic development, tissue regeneration, and cell proliferation. The Shh protein activates the Hh signaling pathway by binding to its receptor Patched (Ptch), which in turn activates the G protein-coupled receptor Smoothened (Smo). This signaling pathway is involved in the directed development of various organs and tissues during embryogenesis, including the central nervous system, limbs, and cardiovascular system. Due to the importance of Shh protein in development and tissue repair, it is also widely studied for its applications in stem cell culture, tissue engineering, and drug development.

This recombinant human Shh is provided in the form of lyophilized powder, with high activity, high purity, low endotoxin, and without any additional tags.

Product Properties

 

Synonyms
HHG1, HHG-1, HLP3, HPE3, MCOPCB5
Accession
Q15465

Source

E.coli-derived Human shh, Cly25-Gly197, with an N-terminal Met.
Molecular Weight
Approximately 19.8kDa.
AA Sequence
IVIGPGRGFG KRRHPKKLTP LAYKQFIPNV AEKTLGASGR YEGKISRNSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDKLNALAI SVMNQWPGVK LRVTEGWDED GHHSEE
SLHY EGRAVDITTS DRDRSKYGML ARLAVEAGFD WVYYESKAHI HCSVKAENSVAAKSGG
Tag
None
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
> 98 % by SDS-PAGE and HPLC analyses.
Biological Activity

The ED50 as determined by inducing alkaline phosphatase production of murine C3H/10T1/2 cells is less than 1 μg/mL, corresponding to a specific activity of > 1.0 × 103 IU/mg. Fully biologically active when compared to standard.
Endotoxin
Less than 1.0 EU/μg of the protein as determined by LAL method.
Formulation
Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom.Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C.
Further dilutions should be made in appropriate buffered solutions.

 

Storage

 

The products are shipped with ice pack and can be stored at-25~-15℃  for 1 year.
7 days, 2 to 8 °C under sterile conditions after reconstitution. 
3 months,-85~-65℃ under sterile conditions after reconstitution.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

Caution

 

1. Avoid repeated freeze-thaw cycles.
2. For your safety and health, please wear lab coats and disposable gloves for operation.
3. For research use only.

Recombinant Human Sonic Hedgehog N-Terminus (Human SHH)_C230493

Product form

SKU: C230493E

$74.00

    • Shipped today? Order within: Feb 17, 2025 17:00:00 -0500
    • Tell a unique detail about this product

    Description

    Shh (Sonic Hedgehog protein) is a signaling molecule and a member of the Hedgehog (Hh) family. It plays a crucial role in physiological processes such as embryonic development, tissue regeneration, and cell proliferation. The Shh protein activates the Hh signaling pathway by binding to its receptor Patched (Ptch), which in turn activates the G protein-coupled receptor Smoothened (Smo). This signaling pathway is involved in the directed development of various organs and tissues during embryogenesis, including the central nervous system, limbs, and cardiovascular system. Due to the importance of Shh protein in development and tissue repair, it is also widely studied for its applications in stem cell culture, tissue engineering, and drug development.

    This recombinant human Shh is provided in the form of lyophilized powder, with high activity, high purity, low endotoxin, and without any additional tags.

    Product Properties

     

    Synonyms
    HHG1, HHG-1, HLP3, HPE3, MCOPCB5
    Accession
    Q15465

    Source

    E.coli-derived Human shh, Cly25-Gly197, with an N-terminal Met.
    Molecular Weight
    Approximately 19.8kDa.
    AA Sequence
    IVIGPGRGFG KRRHPKKLTP LAYKQFIPNV AEKTLGASGR YEGKISRNSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDKLNALAI SVMNQWPGVK LRVTEGWDED GHHSEE
    SLHY EGRAVDITTS DRDRSKYGML ARLAVEAGFD WVYYESKAHI HCSVKAENSVAAKSGG
    Tag
    None
    Physical Appearance
    Sterile Filtered White lyophilized (freeze-dried) powder.
    Purity
    > 98 % by SDS-PAGE and HPLC analyses.
    Biological Activity

    The ED50 as determined by inducing alkaline phosphatase production of murine C3H/10T1/2 cells is less than 1 μg/mL, corresponding to a specific activity of > 1.0 × 103 IU/mg. Fully biologically active when compared to standard.
    Endotoxin
    Less than 1.0 EU/μg of the protein as determined by LAL method.
    Formulation
    Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.
    Reconstitution
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom.Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C.
    Further dilutions should be made in appropriate buffered solutions.

     

    Storage

     

    The products are shipped with ice pack and can be stored at-25~-15℃  for 1 year.
    7 days, 2 to 8 °C under sterile conditions after reconstitution. 
    3 months,-85~-65℃ under sterile conditions after reconstitution.
    Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

     

    Caution

     

    1. Avoid repeated freeze-thaw cycles.
    2. For your safety and health, please wear lab coats and disposable gloves for operation.
    3. For research use only.

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