Synonyms |
TNFRSF6, CD95, Apo I, Fas Antigen
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Accession
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Source
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E.coli-derived Human sFasR/TNFRSF6 protein,Arg17-Asn173.
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Molecular Weight
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RLSSKSVNAQ VTDINSKGLE LRKTVTTVET QNLEGLHHDG QFCHKPCPPG ERKARDCTVN GDEPDCVPCQ EGKEYTDKAH FSSKCRRCRL CDEGHGLEVE INCTRTQNTK CRCKPNFFCN
STVCEHCDPC TKCEHGIIKE CTLTSNTKCK EEGSRSN
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Tag
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None
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Sterile Filtered White lyophilized (freeze-dried) powder.
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Purity |
> 95 % by SDS-PAGE and HPLC analyses.
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Biological Activity
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Fully biologically active when compared to standard. The ED50 as determined by its ability to inhibit the cytotoxicity of Jurkat cells is between 10-15 µg/mL in the presence of 2 ng/mLof rHuFas Ligand.
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Endotoxin
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Less than 1.0 EU/μg of the protein as determined by LAL method.
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Formulation
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Lyophilized from a 0.2 mm filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.
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We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom.Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C.
Further dilutions should be made in appropriate buffered solutions.
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