Description
This gene encodes insulin, a peptide hormone that plays a vital role in the regulation of carbohydrate and lipid metabolism. After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region. Alternative splicing results in multiple transcript variants.
Product Properties
| Synonyms |
Human Proinsulin C-Peptide Analogue, IDDM Protein, Human; IDDM1 Protein, Human
|
|
Accession
|
P01308
|
|
|
3630
|
|
Source
|
E.coli-derived human Proinsulin C-Peptide Analogue protein,Arg55-Arg89
|
|
Molecular Weight
|
|
|
|
RREAEDLQVG QVELGGGPGA GSLQPLALEG SLQKR
|
|
Tag
|
None
|
|
|
Sterile Filtered White lyophilized (freeze-dried) powder.
|
| Purity |
> 95 % by SDS-PAGE and HPLC analyses.
|
|
Biological Activity
|
|
|
Endotoxin
|
Less than 0.1 EU/μg of the protein as determined by LAL method.
|
|
Formulation
|
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
|
|
|
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom.Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C.
Further dilutions should be made in appropriate buffered solutions.
|
Storage
The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
Caution
1. Avoid repeated freeze-thaw cycles.
2. For your safety and health, please wear lab coats and disposable gloves for operation.
3. For research use only.
