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Placenta Growth Factor-2, PGFL
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Source
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E.coli-derived Human PlGF-2 protein,Leu19-Arg131.
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Molecular Weight
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LPAVPPQQWA LSAGNGSSEV EVVPFQEVWG RSYCRALERL VDVVSEYPSE VEHMFSPSCV
SLLRCTGCCG DENLHCVPVE TANVTMQLLK IRSGDRPSYV ELTFSQHVRC ECRPLREKMK
PERRRPKGRG KRRREKQRPT DCHLCGDAVP RR
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Sterile Filtered White lyophilized (freeze-dried) powder.
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Purity |
> 97% by SDS-PAGE and HPLC analyses.
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Biological Activity
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Fully biologically active when compared to standard. The biologically active as determined by its ability
to chemoattract human monocytes using a concentration range of 5.0-50 ng/mL.
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Endotoxin
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< 0.1 EU per 1μg of the protein by the LAL method.
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Formulation
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Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4, with 0.02 % Tween-20.
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We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom.
Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further
dilutions should be made in appropriate buffered solutions.
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