Recombinant Human Placenta Growth Factor-2 (Human PlGF-2) _ C230475

Description

PlGF-2 is an angiogenic factor that belongs to the cysteine-knot superfamily of growth factors. PlGF-2 is expressed in umbilical vein endothelial cells and placenta. It signals through the VEGFR-1/FLT1 receptor, and stimulates endothelial cell proliferation and
migration. PlGF-2 also signals through Neuropilin (NP-1), and can bind with high affinity to heparin. Recombinant Human PlGF-2 is a 34.6 kDa disulfide-linked homodimeric protein of two 152 amino acid polypeptide chains.

 

Product Properties

 

Synonyms

Placenta Growth Factor-2, PGFL

Accession
P49763-3
GeneID
5228

Source

E.coli-derived Human PlGF-2 protein,Leu19-Arg131.
Molecular Weight
Approximately 34.6 kDa.
AA Sequence

LPAVPPQQWA LSAGNGSSEV EVVPFQEVWG RSYCRALERL VDVVSEYPSE VEHMFSPSCV
SLLRCTGCCG DENLHCVPVE TANVTMQLLK IRSGDRPSYV ELTFSQHVRC ECRPLREKMK
PERRRPKGRG KRRREKQRPT DCHLCGDAVP RR

Tag
None
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
> 97% by SDS-PAGE and HPLC analyses.
Biological Activity
Fully biologically active when compared to standard. The biologically active as determined by its ability
to chemoattract human monocytes using a concentration range of 5.0-50 ng/mL.
Endotoxin
< 0.1 EU per 1μg of the protein by the LAL method.
Formulation
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4, with 0.02 % Tween-20.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom.
Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further
dilutions should be made in appropriate buffered solutions.

 

Storage

 

The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

Caution

 

1. Avoid repeated freeze-thaw cycles.
2. For your safety and health, please wear lab coats and disposable gloves for operation.
3. For research use only.

Recombinant Human Placenta Growth Factor-2 (Human PlGF-2) _ C230475

Product form

SKU: C230475E

$74.00

    • Shipped today? Order within: Jan 17, 2025 17:00:00 -0500
    • Tell a unique detail about this product

    Description

    PlGF-2 is an angiogenic factor that belongs to the cysteine-knot superfamily of growth factors. PlGF-2 is expressed in umbilical vein endothelial cells and placenta. It signals through the VEGFR-1/FLT1 receptor, and stimulates endothelial cell proliferation and
    migration. PlGF-2 also signals through Neuropilin (NP-1), and can bind with high affinity to heparin. Recombinant Human PlGF-2 is a 34.6 kDa disulfide-linked homodimeric protein of two 152 amino acid polypeptide chains.

     

    Product Properties

     

    Synonyms

    Placenta Growth Factor-2, PGFL

    Accession
    P49763-3
    GeneID
    5228

    Source

    E.coli-derived Human PlGF-2 protein,Leu19-Arg131.
    Molecular Weight
    Approximately 34.6 kDa.
    AA Sequence

    LPAVPPQQWA LSAGNGSSEV EVVPFQEVWG RSYCRALERL VDVVSEYPSE VEHMFSPSCV
    SLLRCTGCCG DENLHCVPVE TANVTMQLLK IRSGDRPSYV ELTFSQHVRC ECRPLREKMK
    PERRRPKGRG KRRREKQRPT DCHLCGDAVP RR

    Tag
    None
    Physical Appearance
    Sterile Filtered White lyophilized (freeze-dried) powder.
    Purity
    > 97% by SDS-PAGE and HPLC analyses.
    Biological Activity
    Fully biologically active when compared to standard. The biologically active as determined by its ability
    to chemoattract human monocytes using a concentration range of 5.0-50 ng/mL.
    Endotoxin
    < 0.1 EU per 1μg of the protein by the LAL method.
    Formulation
    Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4, with 0.02 % Tween-20.
    Reconstitution
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom.
    Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further
    dilutions should be made in appropriate buffered solutions.

     

    Storage

     

    The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.
    Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

     

    Caution

     

    1. Avoid repeated freeze-thaw cycles.
    2. For your safety and health, please wear lab coats and disposable gloves for operation.
    3. For research use only.

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