Recombinant Human Persephin (Human Persephin) _ C230473

Description

Persephin is a secreted protein belonging to the glial cell line-derived neurotrophic factor (GDNF) family of the TGF-beta superfamily. It shares 38 - 46% amino acid (aa) identity with family members GDNF, neurturin and artemin. Persephin is expressed
at very low levels in most tissues. The 156 aa, 10 - 12 kDa mature protein contains a signal sequence, a pro-domain and a 96 aa mature sequence with several cysteines that are conserved among family members. It circulates as an unglycosylated disulfide-linked
homodimer. Mature human Persephin shares 81% and 80%, 89% and 87% amino acid sequence identity with mouse, rat, bovine and canine Persephin, respectively. Like other GDNF family members, Persephin acts through engagement of GRF alpha 4, a
glycosylphosphatidylinositol (GPI)-linked GDNF receptor family (GRF) member that signals through the receptor tyrosine kinase RET. Persephin is reported to promote both the survival and growth of central dopaminergic and motor neurons, and kidney
development. These effects are correlated with the expression patterns of GFR alpha 4, and RET. Functional GFR alpha 4 isoforms are found only in thyroid, adrenal medulla and portions of the central nervous system, and include GPI-linked, transmembrane and
soluble forms. In vitro, Persephin promotes survival only in neurons which coexpress GPI-linked GFR alpha 4 with RET. This effect does not show a strong correlation to the recruitment of RET in lipid rafts seen with other GDNF family members.

 

Product Properties

 

Synonyms

Persephin; PSP; PSPN

Accession
P06734
GeneID
2208

Source

E.coli-derived Human Persephin, Ala61-Gly156.
Molecular Weight
Approximately 20.5 kDa.
AA Sequence

ALSGPCQLWS LTLSVAELGL GYASEEKVIF RYCAGSCPRG ARTQHGLALA RLQGQGRAHG GPCCRPTRYT DVAFLDDRHR WQRLPQLSAA ACGCGG

Tag
None
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
> 97 % by SDS-PAGE and HPLC analyses.
Biological Activity
The ED50 as determined by a cell proliferation assay using human TT medullary thyroid cancer cells is less
than 10 ng/mL, corresponding to a specific activity of > 1.0 × 10 5 IU/mg. Fully biologically active when
compared to standard.
Endotoxin
< 0.1 EU per 1μg of the protein by the LAL method.
Formulation
Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom.
Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further
dilutions should be made in appropriate buffered solutions.

 

Storage

 

The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

Caution

 

1. Avoid repeated freeze-thaw cycles.
2. For your safety and health, please wear lab coats and disposable gloves for operation.
3. For research use only!

Recombinant Human Persephin (Human Persephin) _ C230473

Product form

SKU: C230473E

$82.00

      • Tell a unique detail about this product

      Description

      Persephin is a secreted protein belonging to the glial cell line-derived neurotrophic factor (GDNF) family of the TGF-beta superfamily. It shares 38 - 46% amino acid (aa) identity with family members GDNF, neurturin and artemin. Persephin is expressed
      at very low levels in most tissues. The 156 aa, 10 - 12 kDa mature protein contains a signal sequence, a pro-domain and a 96 aa mature sequence with several cysteines that are conserved among family members. It circulates as an unglycosylated disulfide-linked
      homodimer. Mature human Persephin shares 81% and 80%, 89% and 87% amino acid sequence identity with mouse, rat, bovine and canine Persephin, respectively. Like other GDNF family members, Persephin acts through engagement of GRF alpha 4, a
      glycosylphosphatidylinositol (GPI)-linked GDNF receptor family (GRF) member that signals through the receptor tyrosine kinase RET. Persephin is reported to promote both the survival and growth of central dopaminergic and motor neurons, and kidney
      development. These effects are correlated with the expression patterns of GFR alpha 4, and RET. Functional GFR alpha 4 isoforms are found only in thyroid, adrenal medulla and portions of the central nervous system, and include GPI-linked, transmembrane and
      soluble forms. In vitro, Persephin promotes survival only in neurons which coexpress GPI-linked GFR alpha 4 with RET. This effect does not show a strong correlation to the recruitment of RET in lipid rafts seen with other GDNF family members.

       

      Product Properties

       

      Synonyms

      Persephin; PSP; PSPN

      Accession
      P06734
      GeneID
      2208

      Source

      E.coli-derived Human Persephin, Ala61-Gly156.
      Molecular Weight
      Approximately 20.5 kDa.
      AA Sequence

      ALSGPCQLWS LTLSVAELGL GYASEEKVIF RYCAGSCPRG ARTQHGLALA RLQGQGRAHG GPCCRPTRYT DVAFLDDRHR WQRLPQLSAA ACGCGG

      Tag
      None
      Physical Appearance
      Sterile Filtered White lyophilized (freeze-dried) powder.
      Purity
      > 97 % by SDS-PAGE and HPLC analyses.
      Biological Activity
      The ED50 as determined by a cell proliferation assay using human TT medullary thyroid cancer cells is less
      than 10 ng/mL, corresponding to a specific activity of > 1.0 × 10 5 IU/mg. Fully biologically active when
      compared to standard.
      Endotoxin
      < 0.1 EU per 1μg of the protein by the LAL method.
      Formulation
      Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4.
      Reconstitution
      We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom.
      Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further
      dilutions should be made in appropriate buffered solutions.

       

      Storage

       

      The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.
      Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

       

      Caution

       

      1. Avoid repeated freeze-thaw cycles.
      2. For your safety and health, please wear lab coats and disposable gloves for operation.
      3. For research use only!

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