Recombinant Human PDGF-AA Protein _ C230477

Description

PDGF is involved in a number of biological processes, including hyperplasia, embryonic neuron development, chemotaxis, and respiratory tubule epithelial cell development. The A-chain homodimers of the platelet-derived growth factor (PDGF AA) are widely
expressed in normal and transformed cells. The mitogenic properties of PDGF AA are well established. FGF-2 and platelet-derived growth factor-A (PDGF-AA) are potent modulators of oligodendrocytes, the main responsible cells for myelin

 

Product Properties

 

Synonyms

PDGF1, PDGF-A, PDGFAA, PDGF-AA

Accession
P04085
GeneID
5154

Source

E.coli-derived human PDGF-AA, Ser87-His211, with an N-terminal Met.
Molecular Weight
Approximately 28.8 kDa.
AA Sequence

MSIEEAVPAV CKTRTVIYEI PRSQVDPTSA NFLIWPPCVE VKRCTGCCNT SSVKCQPSRV HHRSVKVAKV EYVRKKPKLK EVQVRLEEHL ECACATTSLN PDYREEDTGR PRESGKKRKR
KRLKPT

Tag
None
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
>95% by SDS-PAGE analyses.
Biological Activity
The ED50 for this effect is 50-200 ng/mL in a fluorometric assay using the redox sensitive dye. Measured in a cell proliferation assay using NR6R-3T3 mouse fibroblast cells.
Endotoxin
< 0.1 EU per 1μg of the protein by the LAL method.
Formulation
Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, 300 mM NaCl, pH 6.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

 

Storage

 

The products are shipped with ice pack and can be stored at -20℃ for 1 year.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 °C under sterile conditions after reconstitution.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

Caution

 

1. Avoid repeated freeze-thaw cycles.
2. For your safety and health, please wear lab coats and disposable gloves for operation.
3. For research use only.

Recombinant Human PDGF-AA Protein _ C230477

Product form

SKU: C230477E

$179.00

      • Tell a unique detail about this product

      Description

      PDGF is involved in a number of biological processes, including hyperplasia, embryonic neuron development, chemotaxis, and respiratory tubule epithelial cell development. The A-chain homodimers of the platelet-derived growth factor (PDGF AA) are widely
      expressed in normal and transformed cells. The mitogenic properties of PDGF AA are well established. FGF-2 and platelet-derived growth factor-A (PDGF-AA) are potent modulators of oligodendrocytes, the main responsible cells for myelin

       

      Product Properties

       

      Synonyms

      PDGF1, PDGF-A, PDGFAA, PDGF-AA

      Accession
      P04085
      GeneID
      5154

      Source

      E.coli-derived human PDGF-AA, Ser87-His211, with an N-terminal Met.
      Molecular Weight
      Approximately 28.8 kDa.
      AA Sequence

      MSIEEAVPAV CKTRTVIYEI PRSQVDPTSA NFLIWPPCVE VKRCTGCCNT SSVKCQPSRV HHRSVKVAKV EYVRKKPKLK EVQVRLEEHL ECACATTSLN PDYREEDTGR PRESGKKRKR
      KRLKPT

      Tag
      None
      Physical Appearance
      Sterile Filtered White lyophilized (freeze-dried) powder.
      Purity
      >95% by SDS-PAGE analyses.
      Biological Activity
      The ED50 for this effect is 50-200 ng/mL in a fluorometric assay using the redox sensitive dye. Measured in a cell proliferation assay using NR6R-3T3 mouse fibroblast cells.
      Endotoxin
      < 0.1 EU per 1μg of the protein by the LAL method.
      Formulation
      Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, 300 mM NaCl, pH 6.0.
      Reconstitution
      We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

       

      Storage

       

      The products are shipped with ice pack and can be stored at -20℃ for 1 year.
      1 month, 2 to 8 °C under sterile conditions after reconstitution.
      3 months, -20 °C under sterile conditions after reconstitution.
      Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

       

      Caution

       

      1. Avoid repeated freeze-thaw cycles.
      2. For your safety and health, please wear lab coats and disposable gloves for operation.
      3. For research use only.

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