Recombinant Human Neuron-specific Enolase (Human NSE) _ C230457

Description

Neuron specific enolase (NSE), also known as ENO2 or gamma-enolase, is a dimeric, Mg 2+ -dependent enzyme that catalyzes the dehydration of 2-phospho-D glycate (PGA) to phosphoenolpyruvate (PEP) in the glycolytic pathway and catalyzes the reverse
reaction in gluconeogenesis. NSE is a 47.1 kDa member of the Enolase family of enzymes. It is expressed in developing neurons and glia, is known to catalyze the generation of phosphoenolpyruvate, and is suggested to possess neurotrophic activity for neurons, likely through an extracellular mechanism. Human Neuron-specific Enolase is 433 amino acids (aa) in length. The enzymatic site spans most of the length of the molecule. Neuron-specific Enolase exists as both a noncovalently-linked homodimer, or heterodimer with alpha-enolase. Full-length human Enolase 2 shares 99% aa identity with both murine and canine Enolase 2. It shares 83% aa identity with human enolases 1 and 3.

 

Product Properties

 

Synonyms
2-phospho-D-glycerate hydrolyase, ENO2, Enolase 2, gamma-Enolase, Neural enolase, neurone-specific
enolase, Neuron-specific enolase, NSE
Accession
P09104.3
GeneID
2026

Source

E.coli-derived human NSE protein, Ser2-Leu434.
Molecular Weight
Approximately 47.1 kDa.
AA Sequence
SIEKIWAREI LDSRGNPTVE VDLYTAKGLF RAAVPSGAST GIYEALELRD GDKQRYLGKG VLKAVDHINS TIAPALISSG LSVVEQEKLD NLMLELDGTE NKSKFGANAI LGVSLAVCKA
GAAERELPLY RHIAQLAGNS DLILPVPAFN VINGGSHAGN KLAMQEFMIL PVGAESFRDA MRLGAEVYHT LKGVIKDKYG KDATNVGDEG GFAPNILENS EALELVKEAI DKAGYTEKIV
IGMDVAASEF YRDGKYDLDF KSPTDPSRYI TGDQLGALYQ DFVRDYPVVS IEDPFDQDDW AAWSKFTANV GIQIVGDDLT VTNPKRIERA VEEKACNCLL LKVNQIGSVT EAIQACKLAQ
ENGWGVMVSH RSGETEDTFI ADLVVGLCTG QIKTGAPCRS ERLAKYNQLM RIEEELGDEA RFAGHNFRNP SVL
Tag
None
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
>96% by SDS-PAGE and HPLC analyses.
Biological Activity
Testing in progress.
Endotoxin
< 0.1 EU per 1μg of the protein by the LAL method.
Formulation
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4, with 5% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

 

Storage

 

The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

Caution

 

1. Avoid repeated freeze-thaw cycles.
2. For your safety and health, please wear lab coats and disposable gloves for operation.
3. For research use only.

Recombinant Human Neuron-specific Enolase (Human NSE) _ C230457

Product form

SKU: C230457E

$82.00

      • Tell a unique detail about this product

      Description

      Neuron specific enolase (NSE), also known as ENO2 or gamma-enolase, is a dimeric, Mg 2+ -dependent enzyme that catalyzes the dehydration of 2-phospho-D glycate (PGA) to phosphoenolpyruvate (PEP) in the glycolytic pathway and catalyzes the reverse
      reaction in gluconeogenesis. NSE is a 47.1 kDa member of the Enolase family of enzymes. It is expressed in developing neurons and glia, is known to catalyze the generation of phosphoenolpyruvate, and is suggested to possess neurotrophic activity for neurons, likely through an extracellular mechanism. Human Neuron-specific Enolase is 433 amino acids (aa) in length. The enzymatic site spans most of the length of the molecule. Neuron-specific Enolase exists as both a noncovalently-linked homodimer, or heterodimer with alpha-enolase. Full-length human Enolase 2 shares 99% aa identity with both murine and canine Enolase 2. It shares 83% aa identity with human enolases 1 and 3.

       

      Product Properties

       

      Synonyms
      2-phospho-D-glycerate hydrolyase, ENO2, Enolase 2, gamma-Enolase, Neural enolase, neurone-specific
      enolase, Neuron-specific enolase, NSE
      Accession
      P09104.3
      GeneID
      2026

      Source

      E.coli-derived human NSE protein, Ser2-Leu434.
      Molecular Weight
      Approximately 47.1 kDa.
      AA Sequence
      SIEKIWAREI LDSRGNPTVE VDLYTAKGLF RAAVPSGAST GIYEALELRD GDKQRYLGKG VLKAVDHINS TIAPALISSG LSVVEQEKLD NLMLELDGTE NKSKFGANAI LGVSLAVCKA
      GAAERELPLY RHIAQLAGNS DLILPVPAFN VINGGSHAGN KLAMQEFMIL PVGAESFRDA MRLGAEVYHT LKGVIKDKYG KDATNVGDEG GFAPNILENS EALELVKEAI DKAGYTEKIV
      IGMDVAASEF YRDGKYDLDF KSPTDPSRYI TGDQLGALYQ DFVRDYPVVS IEDPFDQDDW AAWSKFTANV GIQIVGDDLT VTNPKRIERA VEEKACNCLL LKVNQIGSVT EAIQACKLAQ
      ENGWGVMVSH RSGETEDTFI ADLVVGLCTG QIKTGAPCRS ERLAKYNQLM RIEEELGDEA RFAGHNFRNP SVL
      Tag
      None
      Physical Appearance
      Sterile Filtered White lyophilized (freeze-dried) powder.
      Purity
      >96% by SDS-PAGE and HPLC analyses.
      Biological Activity
      Testing in progress.
      Endotoxin
      < 0.1 EU per 1μg of the protein by the LAL method.
      Formulation
      Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4, with 5% Trehalose.
      Reconstitution
      We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

       

      Storage

       

      The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.
      Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

       

      Caution

       

      1. Avoid repeated freeze-thaw cycles.
      2. For your safety and health, please wear lab coats and disposable gloves for operation.
      3. For research use only.

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